PR1MA™ Guanidine Thiocyanate

CAS Number: 593-84-0
Molecular Weight: 118.2
Chemical Formula: CH₅N₃ • HSCN
Solubility: Water
Storage Temp: Room Temperature

Protein denaturant used for purifying proteins and nucleic acids.

Toxicity: LD50

Research or further manufacturing use only, not for food or drug use.

• Appearance: White to slightly off white crystalline powder
• Assay (Argentometric Titration): ≥ 99.0 %
• Assay (Ion Chromatography): ≥ 99.5 %
• Chloride Content: ≤ 1.0 %
• Cobalt (Co) : ≤ 5 ppm
• FTIR: Conforms to reference
• Iron: ≤ 5 ppm
• pH: 5.0 - 7.0
• DNase/ RNase: None Detected
• Melting Point: 118 - 121 ^oC
• Solubility: Pass
• Sulfate Ash: ≤ 0.5 %
• Moisture : ≤ 0.5 %
• Grade: Ultra Pure
• Country of Origin: Report

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PR1MA™ Guanidine Hydrochloride

CAS Number: 50-01-1
Molecular Weight: 95.5
Chemical Formula: CH₅N₃-HCl
Solubility: Water
Storage Temp: Room Temperature

Protein denaturant used for purifying proteins and nucleic acids.

Research or further manufacturing use only, not for food or drug use.

• Appearance: White Crystalline Powder
• Assay (Titration, dried basis): ≥ 99.5 %
• Copper (Cu): ≤ 1 ppm
• DNase, RNase, protease: Not Detected
• Free Acid: ≤ 0.01 %
• Heavy Metals (as Pb): ≤ 0.5 ppm
• Identification (IR): Conforms to Known Structure
• Iron (Fe): ≤ 1 ppm
• Loss on Drying: ≤ 1 %
• Melting Point: 185 - 188 ^oC
• pH (10% solution in H₂O @ 25^oC): 5.0 - 7.0
• Residue on Ignition: ≤ 0.05 %
• Solubility (6% Solution in H₂O): Clear
• UVA (230nm, 6M H₂O solution): ≤ 0.14
• UVA (240nm, 6M H₂O solution): ≤ 0.05
• UVA (260nm, 6M H₂O solution): ≤ 0.03
• Zinc (Zn): ≤ 1 ppm

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PR1MA™ qMAX Green qPCR Mix with Blue Tracking Dye

• Superior sensitivity and fast cycling with exceptional results
• Ideal for low copy number templates
• Early Ct values and detection across a broad dynamic range
• Ready to use 2X Master Mix
• Includes PR1MA™ Hot Start Taq Polymerase for greater specificity and accuracy 

Supplied as a ready-to-use 2X master mix, PR1MA™ qMax Green has been engineered for high sensitivity, fast cycling, and excellent reproducibility. PR1MA™ Hot Start Polymerase provides accurate PCR of a variety of templates including low copy number and difficult sequences, while the proprietary qMax Green intercalating dye exhibits higher fluorescence and lower PCR inhibition than other popular green dyes.

These two components are supported by a specially formulated buffer with an exacting combination of salts, PCR enhancers, stabilizers, and pH that results in earlier Ct values and a high specificity across a broad dynamic range.

*Please note these products ship on dry ice. Appropriate shipping charges apply unless otherwise noted on a quote.

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PR1MA™ Proteinase K

CAS Number: 39450-01-6
Solubility: Water
Storage Temp: -20°C

From Tritirachium album. Serine protease for proteolytic inactivation of nucleases during isolation of DNA and RNA.

Shipping Weight: 0 lbs
Shipping Dimensions: 1.25 x 1.75 x 1.25

Specifications

Appearance: White to Off White Lyophilized Solid
Solubility: 50mM Tris-HCl (pH 7.5), 3mM CaCl2, 50% Glycerol
Purity: ≥ 98.0 %
DNase/ RNase: Not Detected
Activity: ≥ 30.0 U/mg
Unit Definition: 1 unit is defined as the amount of enzyme that will liberate 1.0 µmol of tyrosine from casein/min @37°C, pH 7.5
Country of Origin: China

Research or further manufacturing use only, not for food or drug use.

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PR1MA™ Formamide, Super Pure

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PR1MA™ Sodium Acetate, Anhydrous

CAS Number: 127-09-3
Molecular Weight: 82.0
Chemical Formula: C₂H₃O₂Na
Solubility: Water
Storage Temp: Room Temperature

Research or further manufacturing use only, not for food or drug use.

• Appearance: White Powder
• Assay: ≥ 99.0 %
• pH (5% Solution): 7.0 - 9.2
• Solubility: Soluble in Water
• Loss on Drying (120 ^oC): ≤ 1.0 %
• Calcium (Ca): ≤ 0.005 %
• Chloride (Cl): ≤ 0.002 %
• Heavy Metals (as Pb): ≤ 0.001 %
• Insoluble Matter: ≤ 0.01 %
• Iron (Fe): ≤ 0.001 %
• Magnesium (Mg): ≤ 0.002 %
• Phosphate: ≤ 0.001 %
• Sulfate (SO₄): ≤ 0.003 %
• Grade: Meet USP/ACS Specifications

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PR1MA™ Imidazole [Glyoxaline] [1,3-Diaza-2-,4-cyclopentadiene]

Common Name: [Glyoxaline] [1,3-Diaza-2,4-cyclopentadiene]
CAS Number: 288-32-4
Molecular Weight: 68.1
Chemical Formula: C₃H₄N₂
Solubility: Water
Storage Temp: Room Temperature

pKa (25°C): 6.9-7.0
pH range: 6.2-7.8

Research or further manufacturing use only, not for food or drug use.

• Appearance: White to off white crystals
• Assay: ≥ 99.0 %
• DNase: Not Detected
• Identification: Pass Test
• Loss on Drying: ≤ 0.5 %
• Melting Point: 88 - 92 ^oC
• OPTICAL @ 260 NM: ≤ 0.2
• PKa @ 25°C: 6.79 - 7.19
• Protease: Not Detected
• RNase: Not Detected

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PR1MA™ Glycerol

CAS Number: 56-81-5
Molecular Weight: 92.1
Chemical Formula: C₃H₈O₃
Solubility: Water
Storage Temp: Room Temperature

Used in sample preparation and gel formation for polyacrylamide gel electrophoresis. Also used in casting gradient gels, as a protein stabilizer and storage buffer component.

Research or further manufacturing use only, not for food or drug use.

• Appearance: Clear and Colorless Liquid
• Chloride (Cl): ≤ 0.001 %
• Chlorinated Compounds: ≤ 0.003 %
• Color, APHA: ≤ 10.0
• Fatty Acids & Esters (USP): ≤ 1.0 (ml 0.5 N NaOH) %
• Glycerine: ≥ 99.7 %
• Heavy Metals: ≤ 5.0 ppm
• Identification C (GC): Pass
• Limit of Diethylene Glycol & RC: Pass
• Purity (USP): 99.0 - 101.0 %
• Residue on Ignition: ≤ 0.01 %
• Specific Gravity: ≥ 1.259
• Water: ≤ 5.0 %
• Date of Manufacture: Report

PR1MA™ Taq

• Ideal for routine PCR applications as well as genotyping, colony PCR, and fast PCR
• Improved template affinity and solubility for higher enzyme activity and greater yields
• Proprietary buffer optimized for a variety of assay conditions
• Conveniently supplied as a 2X Master Mix or in a 2-tube format of polymerase and separate 5X buffer

PR1MA™ Taq provides superior results for routine applications. Modified to improve DNA-binding, this polymerase offers higher solubility and greater template affinity, resulting in consistently superior performance. PR1MA™ Taq Polymerase exhibits a 5' to 3' nuclease activity, but no 3' to 5' (proofreading) activity and works well with a wide range of DNA templates including GC-rich sequences.

The polymerase is supplied with a 5X buffer containing MgCl2 and a proprietary mix of enhancers (dNTP's not included). For convenience, PR1MA Taq is also available in a ready-to-use 2X Master Mix - just add primers and template DNA. Our "Red Dye" Master Mix incorporates a red-loading dye that allows amplified samples to be loaded directly on an agarose gel.  The red color aids in visualization during pipetting, and higher density of the buffer ensures that the samples will drop into the gel wells.

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PR1MA™ Taq Plus

• Higher fidelity for long PCR amplicons, up to 35 kb
• Ideal for problematic templates
• Better sensitivity and higher activity for low-copy and long PCR
• Enzyme of choice for TA cloning

PR1MA™ Taq Plus, an optimized blend of our Hot Start Taq and a proofreading polymerase, provides 5X better fidelity than wild-type Taq and increased enzyme activity. It is the perfect choice for GC and AT rich templates, low copy number, and long PCR.

PR1MA™ Taq Start Taq DNA Polymerase 

PR1MA™ Taq Plus Master Mix, 2X Concentration

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PR1MA™ qMAX™ Probe qPCR Mix

• Same high efficiency for multiplex and singleplex reactions
• Includes PR1MA™ Hot Start Taq Polymerase for greater specificity and accuracy
• Compatible with popular hydrolysis and beacon probes
• Ready to use 2X mastermix
• Early Ct values and detection across a broad dynamic range 

Optimized for use with TaqMan™, Scorpions^® and molecular beacon probes, qMax Probe qPCR Mix is a ready-to-use formulation for real-time quantitative assays. qMax Probe utilizes PR1MA™ Hot Start Taq Polymerase or robust PCR with a variety of templates. A specially formulated buffer provides optimal conditions for both superior polymerase function and probe detection, resulting in earlier Ct values and a broad detection range. Complicated, multiplex reactions can be performed without any loss in performance or decrease in detection. The 2X mix requires little, if any optimization and can be used with both fast and standard protocols.

PR1MA™ qMax Probe qPCR Mix can be used to detect any DNA template, including genomic DNA and cDNA. Available in high, low and no ROX formulations, qMax Probe is compatible with most qPCR instruments.

*Please note these products ship on dry ice. Appropriate shipping charges apply unless otherwise noted on a quote.

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 PR1MA™ Hot Start Taq

• Exceptional sensitivity for low-copy PCR
• Ideal for multiplex PCR and amplification of GC-rich DNA
• Same enhanced features as PR1MA Taq Polymerase
• Buffer optimized for fast cycling and reproducibility 

Engineered for controlled polymerase activity, PR1MA™ Hot Start Taq is bound with a monoclonal antibody that blocks enzyme activity. This allows reactions to be set up at room temperature without the risk of non-specific amplification.

When samples are ready, the reaction mixture is heated to 95°C to denature the antibody and initiate the reaction. Similar to the standard PR1MA™ Taq, Hot Start Taq is provided with a 5X buffer, or in a ready-to-use 2X Master Mix. The Master Mix can be ordered with or without an incorporated red gel loading dye. The Red Dye Master Mix incorporates a red-loading dye that allows amplified samples to be loaded directly on an agarose gel.  The red color aids in visualization during pipetting, and higher density of the buffer ensures that the samples will drop into the gel wells.

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