PR1MA™ Protein Gel-Loading Dye for SDS-PAGE

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Gel Crusher™ Spin Column

Rapid Gel Fragmentation for DNA Recovery.

Gel Crusher™ Spin Columns provide a fast, efficient, and consistent method for gel fragmentation, replacing traditional crush-and-soak or manual gel breaker steps. Designed with 600 uM filtration pores, these columns enable quick and uniform fragmentation of agarose or acrylamide gel slices, saving time and increasing DNA recovery yields.

Ideal for workflows in molecular cloning, PCR cleanup, restriction digests, DNA sequencing, and labeling protocols, the Gel Crusher™ simplifies the preparation of gel fragments for downstream purification and elution steps.

Simply insert the Gel Crusher™ column into a 2 mL microcentrifuge tube, add your pre-cut gel slice (prepared using a gel cutter or razor blade), and centrifuge at max speed (~15,000 x g) for 5 minutes. The gel will be forced through the 600 uM pores, producing small, uniform fragments that can be easily pipetted using wide-bore tips into NaCl or other solutions.

This process creates a fine slurry that enhances elution efficiency and improves access to the target nucleic acids, significantly reducing incubation and processing time.

Key Features

• Efficiently breaks gel slices into uniform fragments
• Eliminates manual crush-and-soak steps
• Increases DNA yield and recovery efficiency
• Fits standard 2 mL microcentrifuge tubes
• Compatible with standard benchtop centrifuges (up to 15,000 x g)
• 600 uM pore size ideal for agarose or acrylamide gels
• Simplifies downstream processes: elution, filtration, incubation
• For use in PCR, cloning, digestion, sequencing, and more 

Reversible Racks

• Choose 60 well (5 x 12) or 96 well (8 x 12) formats
• Reversible racks, accept 0.5 and 1.5 to 2.0 mL microtubes
• Alphanumeric marking on each well
• Made of polypropylene

Azure ChemiWriter ECL Pen

Chemiluminescent pen for blot annotation

The Azure ChemiWriter ECL Western blot annotation pen is a chemiluminescent marker that enables you to write or draw on your transfer membranes, resulting in a chemiluminescent signal on your markings once substrate is added. Contains a reagent that reacts with HRP substrates to produce chemiluminescence and also a blue dye to allow you to see markings as you write on the Western blot.

Use for tracing visible protein ladders or making identifying marks on your Western blots. Pen has two tips on each end: one fine-tipped for thin markings and one thicker for depositing more of the reagent.

Quantity: 1 pen

Features

Enables you to write or draw on your transfer membranes. A chemiluminescent signal will be detectable from your markings once chemiluminescent substrate is added.

• Use for tracing visible protein ladders or making identifying marks on your blots. Pen has two tips on each end: one fine-tipped for thin markings and one thicker for depositing more of the reagent.
• There are two heads to the pen, a fine point tip and a thicker chisel point tip.
• We recommend using the fine point tip to annotate the blot and mark protein ladders for detection.
• The chisel point of the pen can be used to deposit an increased amount of reagent on the blot for more intense signal.
• For best results we recommend annotating your blot after transfer and before starting your Western blotting protocol.
• Once annotated, the blot may be stored dry or wet, refrigerated or at room temperature.
• Storage: pen should be stored tightly closed at room temperature. ChemiWriter ECL reagent and blue ink markings on the blot are stable for at least three months.

Azure Western Blot Quenching Sheets

Background quenching sheets for use with fluorescent or chemiluminescent samples

Use these quenching sheets under your blot when imaging fluorescent or chemiluminescent Western blots. Sheets reduce background autofluorescence or background signal from contamination in shared imagers, improving the quality of your image.

• For use with fluorescent or chemiluminescent samples.
• Size: 9 x 12.5 cm
• Quantity: 10 Sheets

Features

Use these quenching sheets under your blot when imaging fluorescent or chemiluminescent Western blots.

The sheets reduce background autofluorescence or background signal from contamination in shared imagers, improving the overall quality of your image.

Hoefer® Gradient Makers

Ideal for generating gradients of polyacrylamide, sucrose, and cesium chloride.

• Made of heavy acrylic plastic with high-quality, leak-free Teflon® valves
• Flat base provides stability on the magnetic stir. Comes with a support rod for attaching to a ring stand
• Takes Luer or 4 mm ID tubing. With included adapters, it can also take 2 mm ID or 22-gauge tubing

SG15, SG30, SG50 and SG100 include

• Gradient Maker with Teflon® Valves, 1/ea
• Adapter Barbed Fitting, 1/ea
• Luer Outlet Fitting, 1/ea
• 22-gauge Needle, 1/ea
• Support Rod, 1/ea 

The 500 mL SG500 Gradient Maker is made of two cylindrical acrylic chambers joined and mounted on a flat acrylic base.

• An easy-to-use push-pull valve opens and closes the passage between the reservoir and mixing chambers
• Suitable for forming polyacrylamide gradients or buffer gradients used with medium to small chromatography columns
• Ideal for use with the SE615 Multiple Gel Caster  

SG500 includes

• Gradient Maker with Push-Pull Valve, 1/ea
• Hose Clamps, 4/pk

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Hoefer® Gel Casters

Enable several gradient or homogeneous gels to be cast simultaneously. Choice of multiple-gel casters or dual-gel casters.

Gel Casters for Small Format Vertical Electrophoresis Systems (SE250, SE260, SE300)

Multiple-Gel Casters

• Pour uniform gels from the top; pump gradient gels into an inlet port at the bottom of the unit
• Sandwiches fill at the same time and at the same rate from the same solution
• The result: identical gels
• Glass plate sandwiches seal leak-free without the use of messy grease or tape
• Once cast, gels can be wrapped in plastic and refrigerated for several weeks

Dual-Gel Caster (SE245)

• Cast one or two gels
• Accommodates both 10 x 8 cm and 10 x 10.5 cm plates

*For use with the SE250 and SE260 Series only.
 

Gel Casters for SE600 and SE400 Series Vertical Units   

Multiple-Gel Casters

• SE675 casts up to four 16 cm long gels
• SE615 casts up to ten 16 cm long gels
• Easy to use and leak-free
• Cast homogeneous or gradient gels. Pour homogeneous gels directly into the top of the casting chamber: pump gradient gels through an inlet port at the bottom

Dual-Gel Caster (SE6015)

SE6015 Dual Gel Caster is included when you order an SE600 series unit. It can also be ordered separately.

• Casts two 8, 16, or 24 cm long gels at a time
• Double the capacity with optional divider plates
• Easy to use and leak-free
• Glass Sandwichers seal into the casting 

*For use with the SE600 Series only.

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Hoefer® Combs for SUB15 and SUBHT Submarine Gel Electrophoresis Systems

Available in multiple tooth configurations and thicknesses to suit various sample volumes and well sizes. Hoefer® combs ensure sharp, consistent, well formation for accurate, reproducible results.

MC = Microtiter spacing for use with a multichannel pipette
Sample volume listed is for a 5 mm deep well

For use with SUB15 and SUBHT Submarine Gel Electrophoresis Systems

Specifications
 

Item #	Description	Wells	Teeth	Thickness	Width	Sample Vol.
SUB15-C1-10	SUB15 and SUBHT Comb	10 Wells	10 Teeth	1 mm	12.8 mm	64 uL
SUB15-C15-10	SUB15 and SUBHT Comb	10 Wells	10 Teeth	1.5 mm	12.8 mm	96 uL
SUB15-C2-10	SUB15 and SUBHT Comb	10 Wells	10 Teeth	2 mm	12.8 mm	128 uL
SUB15-C1-16MC	SUB15 and SUBHT Comb	16 Wells	16 Teeth	1 mm	6.4 mm	32 uL
SUB15-C15-16MC	SUB15 and SUBHT Comb	16 Wells	16 Teeth	1.5 mm	6.4 mm	48 uL
SUB15-C2-16MC	SUB15 and SUBHT Comb	16 Wells	16 Teeth	2 mm	6.4 mm	64 uL
SUB15-C1-20	SUB15 and SUBHT Comb	20 Wells	20 Teeth	1 mm	5.9 mm	29 uL
SUB15-C15-20	SUB15 and SUBHT Comb	20 Wells	20 Teeth	1.5 mm	5.9 mm	43 uL
SUB15-C2-20	SUB15 and SUBHT Comb	20 Wells	20 Teeth	2 mm	5.9 mm	58 uL
SUB15-C1-25	SUB15 and SUBHT Comb	25 Wells	25 Teeth	1 mm	3.5 mm	17 uL
SUB15-C15-25	SUB15 and SUBHT Comb	25 Wells	25 Teeth	1.5 mm	3.5 mm	25 uL
SUB15-C2-25	SUB15 and SUBHT Comb	25 Wells	25 Teeth	2 mm	3.5 mm	34 uL
SUB15-C1-30MC	SUB15 and SUBHT Comb	30 Wells	30 Teeth	1 mm	2.5 mm	12 uL
SUB15-C15-30MC	SUB15 and SUBHT Comb	30 Wells	30 Teeth	1.5 mm	2.5 mm	18 uL
SUB15-C2-30MC	SUB15 and SUBHT Comb	30 Wells	30 Teeth	2 mm	2.5 mm	24 uL

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Hoefer® Combs for SUB20 and SUB20C Submarine Gel Electrophoresis Systems

Available in multiple tooth configurations and thicknesses to suit various sample volumes and well sizes. Hoefer® combs ensure sharp, consistent, well formation for accurate, reproducible results.

• MC = Microtiter spacing for use with a multichannel pipette
• Sample volume listed is for a 5 mm deep well
• For use with SUB20 and SUB20C Submarine Gel Electrophoresis Systems

Specifications
 

Item #	Description	Wells	Teeth	Thickness	Width	Sample Vol.
SUB20-C1-16	SUB20 and SUB20C Comb	16 Wells	16 Teeth	1 mm	8.5 mm	35 uL
SUB20-C15-16	SUB20 and SUB20C Comb	16 Wells	16 Teeth	1.5 mm	8.5 mm	55 uL
SUB20-C2-16	SUB20 and SUB20C Comb	16 Wells	16 Teeth	2 mm	8.5 mm	75 uL
SUB20-C1-20MC	SUB20 and SUB20C Comb	20 Wells	20 Teeth	1 mm	7 mm	30 uL
SUB20-C15-20MC	SUB20 and SUB20C Comb	20 Wells	20 Teeth	1.5 mm	7 mm	45 uL
SUB20-C2-20MC	SUB20 and SUB20C Comb	20 Wells	20 Teeth	2 mm	7 mm	60 uL
SUB20-C1-28	SUB20 and SUB20C Comb	28 Wells	28 Teeth	1 mm	5 mm	20 uL
SUB20-C15-28	SUB20 and SUB20C Comb	28 Wells	28 Teeth	1.5 mm	5 mm	30 uL
SUB20-C2-28	SUB20 and SUB20C Comb	28 Wells	28 Teeth	2 mm	5 mm	40 uL
SUB20-C1-40MC	SUB20 and SUB20C Comb	40 Wells	40 Teeth	1 mm	3 mm	13 uL
SUB20-C15-40MC	SUB20 and SUB20C Comb	40 Wells	40 Teeth	1.5 mm	3 mm	19 uL
SUB20-C2-40MC	SUB20 and SUB20C Comb	40 Wells	40 Teeth	2 mm	3 mm	25 uL

 

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Hoefer® Combs for SUB10 Submarine Gel Electrophoresis Systems

Available in multiple tooth configurations and thicknesses to suit various sample volumes and well sizes. Hoefer® combs ensure sharp, consistent, well formation for accurate, reproducible results.

• MC = Microtiter spacing for use with a multichannel pipette
• Sample volume listed is for a 5 mm deep well
• For use with SUB10 Submarine Gel Electrophoresis Systems

Specifications

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Hoefer® Combs for SUB13 Submarine Gel Electrophoresis Systems

Available in multiple tooth configurations and thicknesses to suit various sample volumes and well sizes. Hoefer® combs ensure sharp, consistent, well formation for accurate, reproducible results.

• MC = Microtiter spacing for use with a multichannel pipette
• Sample volume listed is for a 5 mm deep well
• For use with SUB13 Submarine Gel Electrophoresis Systems 

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Hoefer® Combs for HE99X Max Horizontal Agarose Systems

Available in multiple tooth configurations and thicknesses to suit various sample volumes and well sizes. Hoefer® combs ensure sharp, consistent, well formation for accurate, reproducible results.

• All combs require HE91-BK comb back ordered separately
• Preparative combs have 1 preparative well and 2 reference wells
• Sample volume listed is for a 5 mm deep well
• For use with HE99X Max Horizontal Agarose Systems

Specifications

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Hoefer® Combs for SUB6 Submarine Gel Electrophoresis Systems

Available in multiple tooth configurations and thicknesses to suit various sample volumes and well sizes. Hoefer® combs ensure sharp, consistent, well formation for accurate, reproducible results.

• MC = Microtiter spacing for use with a multichannel pipette
• Sample volume listed is for a 5 mm deep well
• For use with SUB6 Submarine Gel Electrophoresis Systems

Specifications
 

Item #	Description	Wells	Teeth	Thickness	Width	Sample Vol.
SUB6-C1-8	SUB6 Comb	8 Wells	8 Teeth	1 mm	4.5 mm	20 uL
SUB6-C15-8	SUB6 Comb	8 Wells	8 Teeth	1.5 mm	4.5 mm	30 uL
SUB6-C2-8	SUB6 Comb	8 Wells	8 Teeth	2 mm	4.5 mm	40 uL
SUB6-C1-12MC	SUB6 Comb	12 Wells	12 Teeth	1 mm	2.5 mm	11 uL
SUB6-C15-12MC	SUB6 Comb	12 Wells	12 Teeth	1.5 mm	2.5 mm	17 uL
SUB6-C2-12MC	SUB6 Comb	12 Wells	12 Teeth	2 mm	2.5 mm	22 uL
SUB6-C1-16	SUB6 Comb	16 Wells	16 Teeth	1 mm	2.2 mm	10 uL
SUB6-C15-16	SUB6 Comb	16 Wells	16 Teeth	1.5 mm	2.2 mm	15 uL
SUB6-C2-16	SUB6 Comb	16 Wells	16 Teeth	2 mm	2.2 mm	20 uL

 

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