This brand is being discontinued and will only be available while supplies last.

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Bullseye Pre-stained Protein Ladder, 10-180 kDa

• Ready to use - no boiling necessary
• Stable at ambient temperature
• 2 reference bands - red at ~75kDa and green at ~25kDa
• Use 5-15µl, depending on the size of the wells
• No freeze-thaw involved
• Broad range ladder

Application: The Bullseye Pre-stained Protein Ladder is suitable for visualizing proteins during electrophoresis without staining and for monitoring transfer onto membrane.

The Bullseye Pre-stained Protein Ladder contains 10 prestained proteins, covering a wide range of molecular weights from 10 to 180kDa. It is designed for monitoring protein separated during polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes and for sizing of proteins. The ladder is supplied in protein gel loading buffer and is ready-to-use. No need to heat, dilute, or add reducing agent before loading. One prominent feature of this protein ladder, is that it remains in the liquid state at the storage temperature; therefore, there is not a freeze-thaw cycle involved, making the ladder very stable.

Contents:
0.1~0.4 mg/ml of each protein in 20 mM Trisphosphate (pH 7.5 at 25°C), 2% SDS, 1 mM reducing reagent, 3.6 M Urea, and stabilizer.

Usage:
For each application, 5-15µl is needed, depending on the size of the wells.

Quality Control:
Tested in polyacrylamide gel electrophoresis and Western Blotting.

Storage: -20 °C.

Note for image:

Western Lightning Plus (NEL103):
·  Twice the sensitivity of standard products 

·  Direct protocol transfer 

·  ~ 1 picogram sensitivity

·  > 2 hr signal duration for re-exposing film

·  Working solution stability 8 hr at room temperature 

·  PVDF or Nitrocellulose membranes 

·  Non-toxic (no msds required)

Western Lightning Pro (NEL120)
·  Mid-range sensitivity, with broad dynamic range

·  Detection of less than 1 picogram amount of protein

·  Long, stable signal > 12 hr for re-exposing film 

·  Stable at room temperature >1 year 

·  Working solution stable for > 24 hr at room temperature

·  Non-toxic (no msds required) 

Western Blot 2.0 with PURITY™

The PURITY™ detection system revolutionizes Western Blotting in three steps.
 

Blocking buffer and secondary antibody are already components of the PURITY™ Reagent. And the washing buffer is also included. The only thing to add is your specific primary antibody.

Benefits:

• 1 simple protocol which makes almost all optimization unnecessary.
• Increased sensitivity and reproducibility of blots.
• Dramatically reduces the number of steps and time required for Western blots.
• Drastically decreases background phenomena.
• Highly reliable.

• Used for agarose electrophoresis of DNA, RNA or nucleic acids
• Contains 3 tracking dyes and 15% Ficoll in a special Tris dye

• Light blue - around 4000bp in 1% agarose
• Indigo - around 600bp in 1% agarose
• Magenta - around 150bp in 1% agarose

• DNase/RNase/Protease free

IB01015
5X RNA Gel Loading Kit

• Reagents for denaturing and loading RNA samples onto a formaldehyde gel, using MOPS as a buffer
• RNA sample is dissolved in 10µl of DEPC water and mixed with 35µl of denaturing solution. Heat the sample to 65°C for 5 min. Once the solution has cooled, add 5µl of loading dye. The sample is now ready to load into the gel.
• DNase/RNase/Protease free

IB01190
2X Protein Loading Dye

• Tracks the migration progression of your sample during polyacrylamide electrophoresis
• Loading dye migrates independently of the samples, making it easier to estimate the migration of proteins
• DNase/RNase/Protease free

IB72120
Xylene Cyanol FF

• Used as a tracking dye at 5kb to monitor the progress of electrophoresis separation
• CAS Number: 2650-17-1
• DNase/RNase/Protease free

IB74040
Bromophenol Blue

• Tracking dye in electrophoretic separations
• Migrates around 0.5kb
• CAS Number: 115-39-9
• Purity: >98.0%

RunBlue SDS Running Buffer (NXB50500) a TEO-Tricine buffer system which provides a separation similar to the MOPS buffer used with BIS-TRIS precast gels. It provides enhanced separation of higher molecular weight proteins. The buffer can be for reduced and non-reduced samples.

RunBlue TGS Blot Buffer(NXB82600) has been specially formulated to provide optimal blot transfer of proteins from RunBlue Precast Gels. Supplied as a concentrate should be diluted by a factor of 10 for use with the RunBlue Dual Run and Blot system or a factor of 20 for use with other blotting methods.