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Actions- Standard plate for the cultivation of adherent cell lines
- Hydrophilic surface with freely accessible carboxyl and hydroxyl groups
- Serum components are easily bound, allowing cells to directly attche and spread out quickly with a better rate of yield
- Transparent bottom and low-volume versions available
- DNase/RNase, pyrogen-free
- Sterile
At a glance: BRANDplates® for cell culture:
cellGrade |
cellGrade plus |
cellGrade premium |
|
Feature: |
Allows cell adhesion |
Enhances cell adhesion |
Poly-D-Lysine |
Application: |
For standard applications in cell culture |
Serum-reduced cultivation for fastidious cell cultures |
Cultivation of cells (including normally adherent types) in suspension |
lipoGrade™ - Hydrophobic Surface
lipoGrade™ plates feature a strongly hydrophobic/lipophilic surface, for the immobilization of biomolecules with strongly hydrophobic regions. They are an alternative to the immunoGrade™ plates for solid phase assays, and are a choice for homogeneous assays with hydrophilic molecules that remain in solution.
- lipoGrade™ assay plates are offered in 96 and 384 well formats; 96-well plates are available in different well geometries
- Treated to bind biomolecules with predominantly hydrophobic regions
- Surface choice to bind lipoproteins, peptides, and other hydrophobic molecules, or for homogeneous assays with hydrophilic molecules that remain in solution
- Non-sterile, polystyrene
- Colors available include transparent, white and black, along with colored plates with transparent bottoms
- Blue alphanumeric coding on 96 well plates
- Free from endotoxins, DNase, DNA, RNase
- Packaged in 10 stacks of 5 plates
- Lids available separately
CellGrade™ for the cultivation of adherent cell lines
- Standard plate for the cultivation of adherent cell lines.
- PS-surface with different chemical groups, such as carboxyl and hydroxyl groups, that are freely accessible.
- Surface is hydrophilic compared with non-treated PS.
- Serum components are easily bound onto the freely accessible chemical groups, allowing an indirect adhesion of cells.
- Clearly distinguishable through the color code: orange, embossed alphanumeric coding for 96-well standard plates.
- Free from endotoxins, DNase, DNA, RNase, non-cytotoxic.
- PS, sterile, with lids.
- BIO-CERT® CELL CULTURE QUALITY: Free of human DNA, RNase, DNase, Pyrogens and non-cytotoxic
Specifications:
cellGrade™ | cellGrade™ Plus | cellGrade™ Premium | |
Feature | Allows cell adhesion (via serum components in media) | Enhances cell adhesion (via protein-like constitution of the surface) | Poly-D-Lysine equivalent surface |
Application | Standard surface for the cultivation of adherent cell lines. | For reduced-serum media cultivation of cells | Cultivation of cells (including normally adherent types) in suspension |
PS-surface with different chemical groups, such as carboxyl and hydroxyl groups, that are freely accessible. | For cultivation of fastidious cell lines. | Poly-D-Lysine equivalent surface, with analogous results regarding growth performance and cell morphology | |
Surface is hydrophilic compared with non-treated PS. | In addition to carboxyl and hydroxyl chemical groups, free amino groups are present on the surface. | Optimal adhesion of cells to the surface reduces cell damage when washing frequently. | |
Serum components are easily bound onto the freely accessible chemical groups, allowing an indirect adhesion of cells. | The surface has a protein-like composition, so cells can directly attach and spread out. | Cultivation of cell lines with the highest demands on their environment. | |
Cells adhere faster, better rate of yield. | Surface suited for serum-free and serum-reduced cultivation of cells. | ||
Sensitive cell lines can be cultivated. | The alternative option to biologically coated surfaces. |
Well-Type | Description | ||
F-Bottom | Flat well bottom. Designed for precise optical measurements, colorimetric determinations, and microscopy applications. | ||
F-Trans Bottom | Transparent flat well bottom. | ||
U-Bottom | Round shaped well bottom. Well suited for stirring and washing samples. | ||
V-Bottom | Conical well bottom. For maximized sample recovery. | ||
C-Bottom | Flat well bottom with curve. |
Poly-D-Lysine equivalent surface
- Poly-D-Lysine equivalent surface, with analogous results regarding growth performance and cell morphology
- Optimal adhesion of cells to the surface reduces cell damage when washing frequently
- Cultivation of cell lines with the highest demands on their environment
- Surface suited for serum-free and serum-reduced cell cultivation
- Alternative option to biologically coated surfaces
- Longer shelf life at room temp than biologically coated surfaces
- DNase/RNase, pyrogen-free
- Sterile
At a glance: BRANDplates® for cell culture:
cellGrade |
cellGrade plus |
cellGrade premium |
|
Feature: |
Allows cell adhesion |
Enhances cell adhesion |
Poly-D-Lysine |
Application: |
For standard applications in cell culture |
Serum-reduced cultivation for fastidious cell cultures |
Plates with surface characteristics for the most demanding cell lines |
All BRANDplates cell culture plates are sterilized by Ã-radiation to ISO 11137 and AAMI guidelines for a SAL of 10-6, meeting the requirements of Ph. Eur. and the USP 29. Plates are DNA-, DNase-, RNase-free, as well as meeting cytotoxic substance levels per DIN EN ISO 10993. Endotoxins are <0.01 EU/mL. All sterile plates are individually wrapped.
For reduced-serum media cultivation of cells
- For the cultivation of fastidious cell lines
- Surface has a protein-like composition so cells can directly attach and spread out
- Cells adhere faster, better rate of yield
- Sensitive cell lines can be cultivated
- DNase/RNase, pyrogen-free
- Sterile
At a glance: BRANDplates® for cell culture:
cellGrade |
cellGrade plus |
cellGrade premium |
|
Feature: |
Allows cell adhesion |
Enhances cell adhesion |
Poly-D-Lysine |
Application: |
For standard applications in cell culture |
Serum-reduced cultivation for fastidious cell cultures |
Cultivation of cells (including normally adherent types) in suspension |
Plate, 96 Round Well V-Bottom 14.40mm Height, 480ul
Specifications:
96-well white frame tissue culture-treated (TC-treated) microplate with clear wells for radiometric and luminescence assays. The clear bottom enables bottom-reading measurements and microscopic visualization of cells.
384, Square, Well
General Features | Product Type: Multi Well Plates |
Number of Wells: 384 | |
Well Shape Top: Square | |
Well Shape Bottom: Pyramid – V | |
Material: Polypropylene | |
Color: Natural | |
Alphanumeric: None | |
Compliance: ANSI/SBS 1-2004 | |
Surface Treatment: None | |
Certified: DNase-/RNase-/Pyrogen-Free | |
Volumes & Dimensions | Overall Height: 14.60mm |
Well Depth: 12.00mm | |
Overall Length: 127.5mm | |
Width: 85.35mm | |
Top Width: 81.20mm | |
Well Diameter Bottom: 2.82mm | |
Well Diameter Top: 3.60mm | |
Top Length: 123.00mm | |
Well Bottom To Deck: 1.7mm | |
Max Volume: 130ul | |
Working Volume: 120ul | |
Volume With Mat Lid: 30ul | |
Performance | Autoclavable: 121C at 15 psi, 15 min. |
Max RCF: 4,000 RCF | |
Max Temperature: 120C | |
Min Temperature: '-80C | |
Shipping & Handling | Quantity: 10 Plates/Pack, 10 Packs/Case |
Case Dimensions (in.): 17.75 x 11 x 7.5 | |
Case Weight (lbs.): 10 |
Protran nitrocellulose membranes are the most frequently specified transfer media in the world for a wide range of applications. Protran membranes are manufactured using 100% pure nitrocellulose to ensure the highest binding capacity possible. Other membranes referred to as "nitrocellulose" may actually contain large amounts of cellulose acetate, which will lower the protein binding capacity. In addition to high binding capacity, Protran nitrocellulose membranes inherently have very low background. The superior surface properties of the membrane guarantees superior signal-to-noise ratios, without the need for stringent washing conditions. Unlike PVDF membranes, Protran nitrocellulose requires no methanol pre-wetting step. This makes it the membrane of choice for proteins that prefer aqueous environments. Prior to transfer, the membrane is simply wet in water and then in the transfer buffer, with no other necessary pre-treatment steps. Protran membranes exhibit the best handling strangth of all pure nitrocellulose membranes.
Features:
- High binding capacity (80-150g/cm2) of proteins and nucleic acids.
- 100% pure nitrocellulose, with no cellulose acetate added, ensures the highest binding capacity possible.
- Compatible with virtually all standard detection methods.
- Low background - NC surface properties guarantee superior signal-to-noise ratios.
- Triton-free matrix with low extractable levels will support the cell growth essential for colony blotting, plaque lifts, and tissue culture applications.
- Available in many sizes and formats to fit different transfer devices and gel chambers.
Grade |
Pore Size |
BA75 |
0.05µm |
BA79 |
0.1µm |
BA83 |
0.2µm |
BA85 |
0.45µm |
The polyester films are a great general purpose seal; ideal for sample protection and elimination of well-to-well sample cross contamination. The acrylic adhesive offers a strong bond that seals tightly on polypropylene and polystyrene plates.
This seal is ideally suited for short term storage, Elisa assays, prevention of sample loss, and general lab usage.
The paper backing of this film is scored on both ends to allow for two handed (precise) placement on the plate.
For PCR applications see our standard PCR film or our Real Time PCR film.
For applications where accessing samples through the seal with pipet tips or a probe is required, see the pierceable aluminum (MID-PCR-200) film.
Certified free of RNase, DNase, Pyrogen, and nucleic-acid contamination.
Length |
146.05 mm |
Length (w/ end tabs removed) |
119.05 mm |
Width |
79.38 mm |
Overall Thickness |
3.1 mil |
Adhesive Thickness |
1.1 mil |
Material |
Polyester |
Adhesive |
Medical Grade Acrylic |
Max. Temperature |
120 °C |
Min. Temperature |
-40 °C |
Primary Usages |
Elisa, Storage or Sample Cover |
AlphaPlates are light gray microplates specially designed to reduce crosstalk in AlphaLISA and AlphaScreen assays. Available in 384- and 1536-well formats and as a shallow-well 384-well plate.