TPP Filtermax Filtration Units

Rapid Filtermax in bottle-filter combo or filter alone beats the competition in speed of filtration and ergonomic design. The overall quadratic shape not only provides a sturdier design and saves refrigerator space with its square bottom, but also houses the premium 0.2µm PES membrane in a larger size.

Key Features

• Large quadratic filter surface of 49 cm2 (250mL filter) and 69 cm2 (500mL filter) is 20% larger than similar Vol units with circular membranes
• Premium PES (Polyether sulfone) filter membrane, pore size of 0.22 µm
• High flow rate
• Low protein binding, excellent chemical resistance
• Secure footprint: unlike round filters, TPP square filters are more stable when filtering and less likely to tip over
• 45mm neck thread on the filter
• Filter tops can fit bottles with 45 mm neck thread
• Quadratic form stores media more efficiently with less wasted space than circular bottles

Additional User-Friendly Advantages

• The tapered form at the neck enables large and small hands a firm and slip-free grip of the rapid-Filtermax system - even when wearing rubber gloves
• One separate packed screw cap and adapter for the vacuum connection hose with an inside diameter of Ø 6 - 10 mm (2.36 - 3.94 inch) is supplied with each system
• Full buyer’s flexibility: choice of a filter set, top only, or flask only, all provided with a 45mm screw thread.

Quality Assurance

• Gamma-sterilized, DNAse/RNAse-Free, and pyrogen-Free
• Individually wrapped in a peel-off plastic wrapper
• Screw cap & vacuum accessories included in each sterile package 

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Specifications

Clipmax Chamber Slide System 10cm2

Grow cells in Clipmax, a combination of a tissue culture flask 10 cm2 with the removable microscope slide (25 x 75mm).

Remove the chamber, fix and stain the cells directly on the microscope slide.  Clipmax is especially designed for cell culture as the slide can be used directly for immunocytochemistry and various cell analyses.

Features of Clipmax:

Optimal OPTICAL CLARITY:

• Crystal clear plastic microscope slide
• Suitable for stainings and especially for immunofluorescence measurements
• Resistant to solvents (eg acetone, ethanol, xylene)

Great for many CELL types

• Activated growth surface for delicate cells
• Removable medium chamber with a âclick❠without the need of a special removeable tool

EASY to remove

• Removable medium chamber with a âclick❠and without the need of a removing tool

Flask for reduced contamination threats

• Filter screw cap for optimal gas exchange with minimal evaporation
• Stackable

Uses only 3.5 ml of media for a media saving, easy to use solution to treating and viewing your cells!

TPP Syringe Filters

Key Features:

• Designed for high flow rate of media or aqueous solutions
• 3 to 5 times faster than standard syringe filters
• Typical starting flow rate at 25°C for water at 2bar (~29psi) is 175mL/min
• 0.22µm or µm PES membrane
• Low protein binding
• Larger filter surface
• Low dead Vol Additional specifications
• Higher operating pressure
• Burst pressure 10bar (~145psi)
• Bubble point 3.7bar (~53psi)

• Standard luer-lock connection

• Housing diameter size 33 mm, Syringe filter rests perfectly on a 50mL centrifuge tube

• Gamma-sterilized, DNAse/RNAse-Free, and pyrogen-Free
• Individually wrapped in peel-off plastic wrapper in convenient card box dispenser

Typical applications:

Sterile filtration of protein solutions, tissue culture media, additives, buffers, and water.

Solutions can be filtered fast due to:

• Large filter surface area, that is increasing the flow rate and throuput
• High operating pressure: a maximum housing pressure of 10 bar (~145 psi).

Bi-directional
The syringe filter units bi-directional support of the filter membrane enables users to filter aqueous solutions in either direction (only ONE direction per use):

• Forward (from the syringe into the container)
• Backward (from the container into the syringe)

The filter unit removes micro organisms, particles, precipitates, and undissolved powders larger than 0.22 micron or 0.45 micron, depending on filter used.
 
Specifications: 
 

TPP Cell Scrapers

TPP Cell Scrapers have a pivoting head that enables the blade angle to be changed effortlessly with a slight twist of the fingers while still in contact with the surface being scraped. This allows for easy and thorough cell retrieval from vessels of all shapes and sizes without having to constantly re-position the scraper or culture vessel.  The TPP exclusive raised knobs on the handle allow for a slip free and secure grip when wearing gloves.

Key Features

• Scraper blade
• Pivots for easy maneuvering without constant lifting and re-positioning

• Made from a especially soft and flexible polyethylene material
• Maintains great contact but won't damage cells

• Small raised knobs on handle for a secure grip

• Scraper sizes provide superior access to all edges of culture vessels of all shapes and sizes
• Allows for 100% cell retrieval

• Sterile, individually packaged in a paper/plastic peel-off wrapper
• Packed in stackable and user friendly TPP boxes

• Lint-free blistering packaging

Specifications
 

Item #	Description	Head Width	Quantity
TP99002	Scraper (24 cm)	1.3 cm	150/cs
TP99003	Scraper (30 cm)	2.0 cm	150/cs
TP99004	Scraper (38 cm)	2.5 cm	100/cs
TP99010	Spatula (20 cm)	1.4 cm	150/cs

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We are sorry some of these products have been discontinued.
GREAT NEWS, we have replacements & TPP is working on new pipettes. 

TPP Serologicals were uniquely designed to be shorter and more ergonomic to permit access into vessels of all shapes and sizes, while providing the end user significant advantages in liquid handing and overall usage. The single piece construction of TPP serologicals 1-25mLs eliminate media drip post-aspiration.

Key features:
Standard serological pipettes: TPP 1mL, 2mL, 5mL, 10mL & 25mL Serologicals

• Accurate Vol 1 - 25 ml
• Highly visible, bidirectional (ascending, descending), and over-Vol graduations
• Single piece construction prevents "pipette drip"
• Smooth interiors, prevent cell hang-up or clumping inside the pipette
• Shorter length reduces arm/back strain of end user
• Excellent optical transparency
• Color coded cotton plugs and dispenser boxes for easy identification

• (25mL - Red, 10mL - Orange,  5mL - Blue, 2mL - Green, 1mL - Yellow)

• Convenient, stackable TPP dispenser box
• Single wrapped in lint-free paper/plastic blister

Serological pipettes with reservoir: TPP 25/40mL & 50/70mL Serologicals

• Manufactured with highest grade PS, extremely break resistant and robust
• Reservoir offers large capacity Vols in a compact, ergonomic design. It augments the total Vol capacity without adding proportionate length to the pipette
• Slender and short form enables ergonomic working conditions in the sterile hood
• Small outside diameter of the tube at the tip results in an excellent accessibility of the large pipettes even in small tissue culture flasks

• 40mL and 70mL max Vols enable larger media aspiration

• Optimized form of the mouthpiece preserves the rubber of the pipette aid

The serological pipettes with reservoir from TPP are molded with highest grade polystyrene. They fit in all commercially available pipette aids with rubber inserts.

Convenient Packaging Design

• Individually wrapped in lint free paper / plastic blister
• Color coded, convenient stackable TPP dispenser box
• Single dispense or convenient opening for dispensing larger number of pipettes

Quality Assurance for all TPP Serologicals:

• Manufactured with the highest grade of polystyrene for greater break resistance
• DNAse/RNAse-free, low endotoxin level <0.1EU/ml
• Sterilized by gamma irradiation
• Individually wrapped in peel-off paper/plastic wrapper

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PR1MA™ Cell Strainers

PR1MA™ cell strainers from MIDSCI™ can be used for many applications including isolating cells and removing clumps or debris. They are compatible with standard 50 mL centrifuge tubes. The exclusive Pestle design improves the manipulation of material in the strainer during operation.

• Polypropylene housing with nylon mesh filter eliminates the need to fabricate filters from gauze, paper, nylon mesh, or other materials
• The elongated lip on the housing extends beyond the edge of the centrifuge tube enabling aseptic handling with forceps
• Extended gripping tab on housing for easy handling
• Available in three porosities: 40, 70 & 100 µm
• Color-coded by mesh porosity for easy identification
• Packaged sterile (gamma irradiated) in individually wrapped peel-to-open paper/plastic wrapper or bulk packed in bags

PR1MA™ Tissue Culture Treated Roller Bottles

• Expanded surface roller bottle increases surface area for cell culture

• Large knurled cap for easy grip

• Vented (HRBV) or non-vented cap (HRBNV) for open or closed systems

• Manufactured from medical grade virgin polystyrene

• Tissue Culture Treated Roller Bottles have uniform hydrophilic surface ideal for consistent cell attachment

• Easy-to-read black printed graduations

• Packaged sterile in individual bags

• Vent caps (RBCAP) feature 0.22µm hydrophobic membrane

PR1MA™ Non-Treated Roller Bottles

• Available in two sizes: 1000 mL Vol with 490 cm² of surface area and 2000 mL Vol with 850 cm² of surface area, treated or non-treated versions

• Large, knurled cap for easy grip

• Vented or non-vented cap for open or closed systems

• Manufactured from medical grade virgin polystyrene

• Tissue Culture Treated Roller Bottles have uniform hydrophilic surface ideal for consistent cell attachment

• Easy-to-read black printed graduations

• Packaged sterile (gamma irradiated) in individual bags

Specifications 

Harvest Fetal Bovine Serum (FBS)

Storage: -20° C

TPP's Packed Cell Vol (PCV) tube enables a quick and reproducible method of cell counting based on cell Vol. A quick centrifuge of the cell suspension pipetted into a PCV tube reveals a packed cell pellet in the PCV capillary, which can be easily read with the aid of an Easy read ruler. This non-subjective method is superior to old-fashioned and labor intensive hemacytometer based counting.

Functional Features:

• Cell suspension sample can be directly quantitated without dilution
• Multiple samples can be assayed simultaneously
• Load and spin as many PCV tubes as your centrifuge rotor holds
• Quick and reproducible
• Cells can be spun down in 1 minute at 2500xg

Ergonomic Features:

• Reproducible measurements
• Graduated capillary model offers immediate quantitation
• For finer quantitation: The Easy Read Ruler  is used with non-graduated PCV tubes
• Eliminates expensive automated counters / fragile slides
• Avoids subjective microscope counting

Quality Assurance:

• Gamma-sterilized, DNAse/RNAse-Free, and pyrogen-Free
• Individually wrapped in peel-off plastic wrapper

Protocol Example:

• Uniformly mix cell suspension collected from TPP  flask/dish/bioreactor
• Pipet 1000µL of cell suspension into a PCV tube
• Spin multiple samples for 1 minute at 2500xg in a standard microcentrifuge
• Record pellet Vol and compare to pre-generated standard curve of cell numbers to packed cell Vol.
• Standard curve needs to be set up only once per cell line
• Pipet pre-determined cell suspension Vols (dilutions) into a hemacytometer
• Calculate and record the number of live cells for each concentration
• Pipet the identical Vols into the PCV tube, centrifuge and record the numeric in the capillary that corresponds to pellet Vol
• Plot the hemacytometer readings vs. the PCV readings for each Vol
• This Standard Curve doesn't need to be redone for the same cell line and moving forward the cell suspension harvested from TPP flasks/dishes/plates only need to be centrifuged in PCV tubes and assayed for cell pellet measurements

High density of cells

• With a very high density of the cells, the samples must not be diluted. You might simply reduce the sample Vol. Samples from the same series should be treated accordingly. 
• For bacteria measurement the parameters have to be adjusted, for example higher g-force, smaller Vol, etc.
• Micro centrifuges with swing-out rotors are preferable.

 

Order#	Description	Quantity
TP87005	PCV Cell Counting Tubes (with capillary graduations only)	50/pk
TP87007	PCV Cell Counting Tubes (no graduations)	50/pk
TP87008	TPP Cap for PCV Tubes	50/pk
TP87010	Easy Read Ruler	1

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• Transparent, nontoxic and inert
• Precise, uniform drop size
• Shatter-proof and unbreakable
• Can be used with LN2 (-196° C)
• Heat Sealable

These unbreakable, one-piece pipettes eliminate contaminated rubber bulbs, broken glass and exposure to infectious materials. They are made of ultra-clear, low density polyethylene (LDPE) and are ideal for microbiology, blood banking, hematology, immunology, and urinalysis. Sterile pipettes are excellent for tissue culture application. Their low-affinity surface reduces sample, cell or protein loss.
 
Whether you make your selection based on length, build draw, pipette capacity or number of drops per ml, one of these ten designs is likely to be suitable for your application. Some have ultra-fine extended tips (P4113, P4117,P4121, P4122, P4133) that allow for extremely precise dispensing and access into the narrowest vessels. They can also be heat sealed for airtight sample storage. Their uniform drop size can be used for reproducible spotting of gels & TLC plates, and for dispensing into test plates, ID strips, PCR tubes or 96 well plates.

CelCulture® CO2 Incubators

• Contamination control with long life ULPA filters and 90°C moist heat decontamination cycle.
• Direct heat & air jacket design with gentle yet effective forced convection.
• Intuitive interfaces and intelligent data and event logger.

The CelCulture® CO2 Incubator is designed and manufactured using the accumulated expertise and knowledge that Esco has developed with over 35 years of world class equipment for laboratory usage. Designed with sample safety, energy efficiency and user-friendliness in mind, the system achieves precise parameter controls, highly effective contamination control and intuitive user interfaces all--supported by Esco's solution-based sales and service representatives worldwide.

Key Benefits

• CelSafe™ Contamination Control System provides unparalleled protection to your precious samples
• SteriSafe™ ULPA filtration enables the chamber air to be maintained at ISO class 5 conditions.
• All input gases are filtered by in-line filters before they enter the chamber to remove impurities and contaminants.
• The incubator's main body is made from electro-galvanized steel with Esco's unique ISOCIDE™ antimicrobial coating.
• SwiftCon™ moist heat 90°C decontamination cycle operates overnight to eradicate contamination in the unlikely event it occurs. At the end of the cycle, the chamber is dry and ready to use.
• CelAlert™ Alarm System reminds users of important tasks needed to protect their cultures
• CO2 tank depletion reminder in addition to CO2 tank-low alarm.
• ULPA-reminder will alert user to replace ULPA filter

VivoCell™ Precise Temperature, CO2 Control and High Humidity

• Best uniformity and control among available CO2 Incubators
• Fast CO2, temperature, and humidity recovery without overshoot.
• Direct heat design for rapid recovery, with 8 heaters grouped into 3 zones.
• Air jacket provides isolation against ambient temperature fluctuations.
• Filtered air circulates across water pan accelerating humidifying process and flows gently around culture plates, causing no disturbance to cell culture.
• Blower automatically stops when door is opened, to minimize mixing of chamber and room air. VentiFlow™ forced convection. accelerates recovery of chamber air to ISO Class 5 Cleanliness after door closing to prevent contamination.

Easy-to-Clean

• Chamber is made of stainless steel chamber with rounded corners enabling efficient and rapid cleaning.
• Ductwork, plenums and shelves are removable without tools for faster and easier operator maintenance.
• Glass door and inner gasket are removable without tools