Incu-Shaker™ - Stackable Shaking Incubator

Innovative features and outstanding cell growth properties make the TPP Tissue Culture Flasks the ideal choice for adherent cell culture in academia and industry.
 

• Cell growth surface

• Uniform flat growth surface promotes cell growth without clumping

• Growth area is opto-mechanically treated to promote cell adhesion and growth

• Lifted base
• Stable, slip-free stacking of multiple TPP flasks
• Allows for air flow between flasks to prevent condensation buildup
• Resulting in uniform temperature and CO2 distribution

• Crystal clear transparency for great microscopy images!

• Geometry of the neck reduces risk of media slop over

• Geometry of the neck guarantees total access of TPP pipettes, TPP scrappers and the TPP spatula. No dead corners = 100% cell retrieval.

• White and accurate Vol graduations for optic control of the filling Vol.

• Highly visible bilateral marking area

• Vented cap settings for "on" at 12 o'clock position and "off" at 3 o'clock position.

Additional Advantages

Flasks are available in either filter cap or vented cap:

The TPP Click

1. A click and the <> screw cap is in the <> aeration-position.
Visual control: the slightly raised rectangle is at 12 o'clock.
2. Turn <> screw cap clockwise 90° to close the flask gas-tight closed.
Visual control: the slightly raised rectangle is at 3 o'clock.

Filter Membrane in Filter Screw Cap
The filter screw caps feature a hydrophobic PTFE membrane with a pore size of 0.22 µm. This offers protection - as the hydrophobic characteristics repel any fluid - with optimal gas exchange at the same time.

Quality Assurance:

• Made from optically clear polystyrene for excellent viewing
• Sterile, packaged in gamma irradiated, sterile sleeves
• Certified DNAse/RNAse, nucleic acid, and pyrogen-free

Key Features:

• Vents provide airflow between stacked dishes for consistent growth conditions and prevention of Condensation
• Surface treatment of the growth area developed by TPP optimally enhances the proliferation of the cells
• Serrated grip ring on lid prevents accidental drops or lid lift offs in unsterile conditions.
• Double marking areas - yellow and frosted marking spots for easy labeling.
• Stacking ring on the lid and corresponding ridge on the base provide an extremely secure stacking feature
• Numeric "clock" markings (12, 3, 6, 9) for quick orientation during microscopy.
• Crystal clear clarity for great microscopy images.

Consistent Growth Conditions

• Air vents in the dish base allow airflow between stacked dishes for consistent air flow, temperature distribution, and prevention of condensation.
• Both features provide consistent growth conditions even when dishes are stacked.
• Outstanding flatness of surface promotes uniform cell growth without clumping.

Notable extra features

• Tissue culture dishes are designed for manual handling.
• The yellow inscription field on the lid and the frosted inscription field on the base enable a defined positioning of the lid.
• The side walls of the dish are not treated for tissue growth

Quality Assurance:

• Made from optically clear polystyrene for excellent viewing
• Sterile, packaged peel-off sleeves
• Certified DNAse/RNAse, nucleic acid, and pyrogen-free

Please call us for sample!

TPP TubeSpin® Bioreactors

Suspension cells ranging from immune cells to insect cells and microorganisms like E.coli to yeast can be cultured and centrifuged in TubeSpin® Bioreactors, eliminating the need for transferring before spinning down.
 
Features:

• working Vol 1-35mL
• easy to start small suspension cell cultures from frozen
• ergonomic use of bacterial and yeast cultures -- static and shaking
• conical bottom
• 0.2µM filter membrane
• Sterile gas exchange
• 5 openings
  • (A, B, C, D, E) of different size above the gas permeable, sterile PTFE filter of the screw cap
• Openings can be sealed to adjust gas exchange according to need
• Fits in standard 15ml centrifuge rotors
• Saves costs of culture start-ups in erlenmyer flasks and then transfer to conical tubes
• Culture and spin in a single tube

Advantages:

• Great gas exchange throughout the culture
• Defined ventilation
• Flexible working Vol with one type of tube
• No transfer stage - Spin down in the same tube
• Large-scale parallel experiments
• Rapid sampling following centrifugation, and the tube is disposable
• No internal stirring mechanism to damage cells or produce foam
• Less incubator or hood space needed; when compared to a T75
• Less plastic usage and less plastic waste
• Easier withdrawal from tube than a flask

Bacterial and Microbiological Cultures
TPP TubeSpin Bioreactors are ideally suited for the culture of bacteria, yeast, and other microbiological organisms in suspension.  The simple, disposable format offers convenience and time-savings over traditional glass vessels such as test tubes which must be washed and autoclaved before each use.  Both aerobic and anaerobic cultures can be grown by selectively covering the holes in the 0.22um filter cap.  1-35mL of culture can be grown in each TubeSpin bioreactor, offering the flexibility to perform mini plasmid preps, small-scale candidate screens, or other downstream applications.

The simple 50mL tube design contains no internal mixing or stirring mechanism which minimizes cell shearing and foam formation, and agitation is provided by shaking in a CO2 shaking incubator. In this manner, hundreds of cell cultivations can be performed quickly and efficiently in parallel.

Water loss
If small culture Vols are incubated over a longer period of time, the loss of water influences the growth of cells.

By the closure of one or several openings above the gas permeable and sterile PTFE membrane, this water loss can be minimized.

Sterile gas exchange is guaranteed through the 0.22 µm pore size PTFE filter membrane.

Please call us for sample!

Literature and comprehensive information about shaking technology can be found in the "Forum Shaking Technology", a cooperation of TPP with competent partner companies.

Features of TPP Tissue Culture Flask with re-closable Lid 115 / 150 cm2:

• Multi-use re-closable liquid-tight lid
• Complete access to flask chamber from above
• Safe, stable, slip-free stacking of multiple flasks
• Optionally available with barriers with additional safety zone

It is important that only filter screw caps are employed. An internal pressure of more than 0.03 bar (0,00043 psi) in the flask without pressure equalization might raise the lid and result in leakage.

Features of TPP Tissue Culture Flask with peel-off Foil 25 - 300 cm2:

• Available in all sizes: 25 - 300 cm2
• Large pinched flap for a firm grip
• Foil is peeled off before access to the cultivation chamber
• Strong welding of peel-off foil
• Multi-layer foil material
• Stacking ring offers stable and slip-free stapling of several flasks
• Optionally available with barrier

This flask cannot be re-closed after the foil has been peeled-off.
The foil is welded securely to the polystyrene carrier.
It is important to use only filter screw caps. An internal pressure of more than 0.03 bar (0,00043 psi) in the flask without pressure equalization might raise the foil and result in leakage.
 
Quality Assurance:

• Made from optically clear polystyrene for excellent viewing
• Sterile, packaged in gamma irradiated, sterile sleeves
• Certified DNAse/RNAse, nucleic acid, and pyrogen-free

TPP Tissue Culture Tubes

Features of the unique tissue culture flat tube 10:

• Multifunctional: cultivation, incubation, examination under microscopy, centrifugation...
• Conical shape of the tube eases the removal of the pellet
• 10 cm² surface activated growth area with new surface quality resulting in crystal clear transparency
• Large opening for complete access of pipettes and scrappers
• Great clarity for visual control with inverse microscopes
• Sloped and flattened upper side results in reduced refraction
• Centrifugation in a 50 mL standard adapter at 1,200 g
• Segmental packaging of 4 protects the individual tubes of scratches during transportation and handling
• Bag slits with laser perforation for easy opening mechanism

The corresponding TPP tissue culture rack fits 8 x tubes in a horizontal position for storage in a CO₂ incubator.

Features of the tissue culture tube 20:

• 20 cm² surface activated growth area
• Growth area over a length of 50 mm
• Volume graduations up to the tip
• Specially formed 10-sided comb from «VENT» screw cap with “Click” avoids inadvertent rolling and enables regaining previous positions
• A click to the lock position and the «VENT» screw cap is in the aeration position, which guarantees constant airflow even when the tube is in a vertical position.
• Fits a 15 mL standard tube adapter, and can be spun up to 1,200 g
• Convenient re-closable zipper bag with laser perforation

Please call us for a sample!

PR1MA™ Plates-Permeable Cell Culture Inserts

• Permeable Cell Culture Inserts mimic in vivo conditions
• Inserts center in wells, preventing membrane from touching sides and wicking between insert and side wall
• Membrane remains suspended above well bottom, allowing co-culture: grow one cell line inside insert, and another below in the well
• Ideal for drug transfer assays, transport studies, migration assays, and cell-cell interaction studies
• Transfer cultures to fresh media by moving the insert to a new plate
• 0.4 µm pore size is ideal for co-culture and drug transport studies
• 3.0 µm and 8.0 µm pore size are ideal for angiogenesis and chemotaxis experiments
• For more help with membrane selection, please contact CELLTREAT Scientific Products
• Non-Treated Plates sold separately; carrier plate is Tissue Culture Treated which encourages cell adhesion

Specifications 

Glass Bottom Plates

• Convenient live cell viewing without needing to transfer
• Optically clear borosilicate glass for exceptional viewing
• Applications include: cell culture and imaging, live cell observation and specialty microscopy such as confocal high resolution and contrast
• Constructed with noncytotoxic medical adhesive
• Round cover glass also available for use with glass bottom plates

Glass Bottom Dish

• Recessed, consistent borosilicate glass bottom is optically clear, and provides micro well ideal for cell culture and microscopy or imaging
• Non-cytotoxic medical adhesive does not inhibit cell growth
• 30mm Dish lid has molded spacers to allow gas exchange and a stacking ring for increased stability

Cover Glass

• High-quality, optically clear borosilicate glass cover slips are ideal for specialty microscopy and imaging
• Ideal for use with dishes and multiple well plates and anywhere a square cover glass is unsuitable
• Consistent flatness and scratch-free
• Resistant to organic solvents
• Packaged in a hinged plastic storage case
• 15mm Round Cover Glass can be used with a 24 well glass bottom plate (Part No. 229125)
• 20mm Round Cover Glass can be used with a 12 well plate or 30mm glass bottom dish (Part No. 229632)
• 25mm Round Cover Glass can be used with a 6 well glass bottom plate (Part No. 229107)
• Cover Glass is #1.5 thickness

Chambered Cell Culture Slides

• Cells grow directly on the slide without any need for future transfer
• Ideal for growing and viewing cells under a microscope on a standard 25mm x 75mm slide
• Uniform hydrophylic surface is ideal for consistent cell attachment
• Slides are labeled with numeric markings
• Packing trays can be used as incubation racks in CO2 incubators
• Adhesive free

PR1MA™ Centrifuge Tube Filter Systems

• Eliminate a sample transfer step by filtering directly into a 50mL tube
• Polyethersulfone (PES) membrane for high flow and low binding
• One Centrifuge Tube Stand included with each case of 50mL Centrifuge Tube Filter Systems
• Centrifuge tube Stands are manufactured from polypropylene and are autoclavable, reusable and recyclable
• Centrifuge Tube Stand will hold one 50mL tube, three 15mL tubes and three microcentrifuge tubes (1.5mL)

Specifications
 

TPP Filtermax Filtration Units

Rapid Filtermax in bottle-filter combo or filter alone beats the competition in speed of filtration and ergonomic design. The overall quadratic shape not only provides a sturdier design and saves refrigerator space with its square bottom, but also houses the premium 0.2µm PES membrane in a larger size.

Key Features

• Large quadratic filter surface of 49 cm2 (250mL filter) and 69 cm2 (500mL filter) is 20% larger than similar Vol units with circular membranes
• Premium PES (Polyether sulfone) filter membrane, pore size of 0.22 µm
• High flow rate
• Low protein binding, excellent chemical resistance
• Secure footprint: unlike round filters, TPP square filters are more stable when filtering and less likely to tip over
• 45mm neck thread on the filter
• Filter tops can fit bottles with 45 mm neck thread
• Quadratic form stores media more efficiently with less wasted space than circular bottles

Additional User-Friendly Advantages

• The tapered form at the neck enables large and small hands a firm and slip-free grip of the rapid-Filtermax system - even when wearing rubber gloves
• One separate packed screw cap and adapter for the vacuum connection hose with an inside diameter of Ø 6 - 10 mm (2.36 - 3.94 inch) is supplied with each system
• Full buyer’s flexibility: choice of a filter set, top only, or flask only, all provided with a 45mm screw thread.

Quality Assurance

• Gamma-sterilized, DNAse/RNAse-Free, and pyrogen-Free
• Individually wrapped in a peel-off plastic wrapper
• Screw cap & vacuum accessories included in each sterile package 

Please call us for a sample! 

Specifications

Clipmax Chamber Slide System 10cm2

Grow cells in Clipmax, a combination of a tissue culture flask 10 cm2 with the removable microscope slide (25 x 75mm).

Remove the chamber, fix and stain the cells directly on the microscope slide.  Clipmax is especially designed for cell culture as the slide can be used directly for immunocytochemistry and various cell analyses.

Features of Clipmax:

Optimal OPTICAL CLARITY:

• Crystal clear plastic microscope slide
• Suitable for stainings and especially for immunofluorescence measurements
• Resistant to solvents (eg acetone, ethanol, xylene)

Great for many CELL types

• Activated growth surface for delicate cells
• Removable medium chamber with a âclick❠without the need of a special removeable tool

EASY to remove

• Removable medium chamber with a âclick❠and without the need of a removing tool

Flask for reduced contamination threats

• Filter screw cap for optimal gas exchange with minimal evaporation
• Stackable

Uses only 3.5 ml of media for a media saving, easy to use solution to treating and viewing your cells!