PR1MA™ MS2 Phage

PR1MA™ MS2 is an E. coli bacteriophage with a single-stranded RNA genome of 3569 nucleotides protected from nuclease degradation by a capsid of 180 coat protein monomers. This virus is a Biosafety Level 1 organism, not pathogenic to humans. These properties make MS2 phage useful as a process control in nucleic acid-based amplification techniques like RT-PCR, RT-LAMP, particularly those that involve viral RNA extraction.

• Biosafety level 1
• Lysis: 65°C for 20 minutes or by standard RNA extraction
• Storage temperature: 4°C 

Buffer composition

• 10 mM Tris-HCl
• 0.1  mM EDTA
• pH = 8.0 

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.
 

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PR1MA™ RNA Controls

Looking for safe assay controls for highly infectious viruses or foreign animal diseases?

MIDSCI™ offers a selection of PR1MA™ RNAs – nuclease-resistant, single-stranded RNAs, suitable as process controls for RNA extraction from various sample matrices. These specially engineered, non-infectious, MS2 phage-like particles protect their contents from degradation by nucleases and can package sequences of up to 1.5 kb from viruses such as SARS-CoV-2, foot-and-mouth disease virus, and human immunodeficiency virus.
 

Amount: Between 1E6 or 1E10 copies (cp)
Concentration: Between 1E7 or 1E10 cp / mL, respectively
Volume: 0.1 and 1 mL respectively
Lysis: 65° C for 5 minutes in the RT step or by standard RNA extraction
Storage temperature: 4°C

Buffer composition

• 10 mM Tris HCl
• 100 mM NaCl
• 1 mM MgCl2
• 0.1% gelatin
• pH = 7.0 

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.

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PR1MA™ RNase Inhibitor

PR1MA™ RNase Inhibitor (RI) is a 50 kDa protein that specifically inhibits RNases A, B, and C by binding noncovalently in a 1:1 ratio at high affinity. It can be used in isothermal amplification and molecular diagnostic assays, cDNA synthesis, and other applications where RNA stability is important. It is ineffective against RNase1, T1, S1 Nuclease, or RNase H. It has no inhibition of polymerase activity when used with Taq DNA polymerase, AMV, M-MuLV, HIV reverse transcriptases, or phage RNA polymerases.

Storage Buffer

• 50% glycerol
• 10 mM Tris-HCl
• 50 mM KCl
• 8 mM DTT
• pH = 7.5 

*To prevent the release of ribonuclease from RNase Inhibitor, temperatures greater than 50°C and high concentrations of denaturing reagents such as urea should be avoided.

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.

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PR1MA™ Taq DNA Polymerase 1X Master Mix

PR1MA™ DNA polymerase 1X Master Mix saves time and cost by enabling direct PCR amplification of unpurified templates. It contains a recombinant, truncated (lacks 5’ to 3’ exonuclease activity), highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme is thermostable up to 98°C for polymerase chain reaction assays and is provided as a complete reaction master mix consisting of reaction buffer, dNTPs, MgCl2, and loading dye and only requires the addition of primers and DNA template. Once PCR is complete, the reaction products can be loaded directly into an agarose gel for analysis.

• Lacks exonuclease activity
• Thermotolerant up to 98°C
• Inhibitor Resistant
• Ideal for colony PCR, genotyping, and GC-rich templates
• Storage temperature: -20°C 

Important note: Please be sure to use the buffer provided with this product to ensure optimal results.

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.

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PR1MA™ β - Agarase

PR1MA™ β - Agarase has a high tolerance to inhibitors in electrophoretic buffers such as TBE and TAE, eliminating the need to perform a buffer exchange step before digestion. PR1MA™ β - Agarase has higher thermal stability than other commercially available β - agarases, with a broad range of activity between 42°C and 50°C. Higher temperature digestion reduces the chances of residual agarose gelling at the end of the reaction, resulting in higher recovery of DNA or RNA.

Benefits

• Complete digestion of agarose, with no agarose fragments, left after the digestion
• Obtain DNA or RNA faster - no buffer exchange needed, with direct digestion in TAE or TBE
• Concentration: 1000 u / mL
• Operating temperature: 42°C to 50°C
• Recommended temperature: 50°C
• Storage temperature: -20°C 

Storage Buffer

• 50% glycerol
• 50 mM Tris-HCI
• 50 mM KCI
• 1 mM DTT
• 0.1  mM EDTA
• pH = 7.5 

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.

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PR1MA™ T4 Gene 32 Protein

PR1MA™ T4 Gene 32 Protein (T4 gp32) is a single-stranded DNA binding (ssDNA) protein required for E. coli bacteriophage T4 replication. It binds and stabilizes ssDNA structures which facilitates electron microscopic examination, and has also been shown to improve restriction digests, improve T4 DNA polymerase activity, and increase the yield of PCR reactions, including those with long amplicons. 

• Optimal temperature: 37°C
• Heat inactivation: 65°C for 20 minutes
• Storage temperature: -20°C
• 10X T4 gp32 reaction buffer included

Buffer composition

• 50% glycerol
• 50 mM Tris-HCl
• 50 mM KCl
• 1 mM DTT
• 0.1  mM EDTA
• 0.1% Tween-20
• pH = 7.5 

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established. 
 

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Freedom Rocker™ BotBlot® 

The Freedom Rocker™ BlotBot® is an automated blot processor. The fully programmable blot processing station automates the tedious incubation and washing steps required to process blots and gels, thus saving time and money through increased productivity. All trays receive the same reagent or wash buffer at the same time, in equal volumes, ensuring consistency.

Simply dispense individual reagents (in volumes as low as 3.5 mL) into appropriate trays and recover them separately. This allows the conservation of primary antibodies. The control panel has a large graphic display and intuitive layout making it easy to program protocols. These protocols can include different rocking speeds and times for each step. 

BlotBot® with a clear window can be employed when using reagents that are not light-sensitive in western blots. BlotBot® with a blackout window is recommended when using fluorescent reagents in western blots to preserve their integrity.

• Automated delivery and removal of buffers/reagents
• Dispense and recover different antibodies or reagents in each tray
• Only 3.5 mL or reagent per blot is needed due to specially designed trays
• Reagent recovery
• Easy to use-just plug in
• Quiet, no air or vacuum connections necessary
• Drip-proof quick-release connections
• 2-year warranty

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Hoefer® miniVE Vertical Protein Electrophoresis and Blotting System

The SE300 minVE is a sturdy, compact vertical protein electrophoresis unit that also performs electrotransfer (blotting). 

• Quick, excellent results using minimal buffer
• Passive cooling produces gels with straight lanes and sharp bands
• Cast your own or use precast gels from a variety of manufacturers
• Transfer up to 4 gels in 45 minutes with only 300 mL buffer using optional SE302 Blot Modules 
• Designed for use with a wide variety of precast gels

Hoefer® miniVE Complete Unit (SE300-10A-10)

• Lower Buffer Chamber (tank), 1/ea
• Gel Modules with Gaskets, 2 assemblies
• Safety Lid with High Voltage Leads, 1/ea
• Glass Plates, Rectangular, 10 x 10.5 cm, 3/pk
• Glass Plates, Notched, 10 x 10.5 cm, 3/pk
• Combs, 10-well, 1.0 mm thick, 2/pk
• T-Spacers, 1.0 mm thick, 4/pk
• Well-Locating Decals, 2/pk
• Wonder Wedge Plate Separation Tool, 1/ea
• Gel Seal, 1/ea

Hoefer® miniVE Basic Unit (SE300)

The same as above without the combs, spacers, and glass plates. For use with precast gels or order two SE211A combs, two sets of 10.5 cm long spacers, and 10 x 10.5 cm rectangular and notched glass plates separately.

Hoefer® miniVE Blot Module (SE302)

• Blot Module, 1/ea
• Blotter Paper, 25 sheets/pk
• Dacron Sponges, 6 mm thick, 4/pk

Specifications
 

Plate Size (W x H)	3.94 x 4.13 In or 3.94 x 3.15 In. | 10 x 10.5 cm or 10 x 8 cm
Spacers	0.75 mm, 1.0 mm, 1.5 mm
Max. Power	1000 V, 500 mA, 50 W
Max. Temp.	113ºF | 45°C
Indoor Use	39.2 to 104ºF | 4 to 40°C
Humidity	Up to 80%
Dims (D x W x H)	5.51 x 12.60 x 14.57 In. | 14 x 32 x 37 cm
Weight	4.85 In. | 2.20 kg

SealPlate® RollMate™ Pre-scored Film

EZcap™FoilStrips

AlumaSeal® CS™