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PR1MA™ MS2 Phage

 

PR1MA™ MS2 is an E. coli bacteriophage with a single-stranded RNA genome of 3569 nucleotides protected from nuclease degradation by a capsid of 180 coat protein monomers. This virus is a Biosafety Level 1 organism, not pathogenic to humans. These properties make MS2 phage useful as a process control in nucleic acid-based amplification techniques like RT-PCR, RT-LAMP, particularly those that involve viral RNA extraction.

  • Biosafety level 1
  • Lysis: 65°C for 20 minutes or by standard RNA extraction
  • Storage temperature: 4°C 

Buffer composition

  • 10 mM Tris-HCl
  • 0.1  mM EDTA
  • pH = 8.0 

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.
 

Proudly made in the USA! Click here for more made in America products!
 

Item#:
ASPRIMAMS2PHAGE
 
Safety Sheet for PR1MA MS2 Phage
Specs for for PR1MA MS2 Phage


PR1MA™ RNase Inhibitor

 

PR1MA™ RNase Inhibitor (RI) is a 50 kDa protein that specifically inhibits RNases A, B, and C by binding noncovalently in a 1:1 ratio at high affinity. It can be used in isothermal amplification and molecular diagnostic assays, cDNA synthesis, and other applications where RNA stability is important. It is ineffective against RNase1, T1, S1 Nuclease, or RNase H. It has no inhibition of polymerase activity when used with Taq DNA polymerase, AMV, M-MuLV, HIV reverse transcriptases, or phage RNA polymerases.

 

Storage Buffer

  • 50% glycerol
  • 10 mM Tris-HCl
  • 50 mM KCl
  • 8 mM DTT
  • pH = 7.5 

*To prevent the release of ribonuclease from RNase Inhibitor, temperatures greater than 50°C and high concentrations of denaturing reagents such as urea should be avoided.

 

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.

 

Proudly made in the USA! Click here for more made in America products!
 

Item#:
ASPRIMARNASEI
 
Specs for for PR1MA RNAse Inhibitor
Safety Sheet for PR1MA RNAse Inhibitor


PR1MA™ β - Agarase

 

PR1MA™ β - Agarase has a high tolerance to inhibitors in electrophoretic buffers such as TBE and TAE, eliminating the need to perform a buffer exchange step before digestion. PR1MA™ β - Agarase has higher thermal stability than other commercially available β - agarases, with a broad range of activity between 42°C and 50°C. Higher temperature digestion reduces the chances of residual agarose gelling at the end of the reaction, resulting in higher recovery of DNA or RNA.


Benefits

  • Complete digestion of agarose, with no agarose fragments, left after the digestion
  • Obtain DNA or RNA faster - no buffer exchange needed, with direct digestion in TAE or TBE
  • Concentration: 1000 u / mL
  • Operating temperature: 42°C to 50°C
  • Recommended temperature: 50°C
  • Storage temperature: -20°C 

Storage Buffer

  • 50% glycerol
  • 50 mM Tris-HCI
  • 50 mM KCI
  • 1 mM DTT
  • 0.1  mM EDTA
  • pH = 7.5 

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.


Proudly made in the USA! Click here for more made in America products!
 

Item#:
ASAGARASE
 
Specs for for PR1MA Agarase
Safety Sheet for PR1MA Agarase
Kit, 2 Preps

The I-Blue MINI Plasmid Kit was designed for rapid isolation of plasmid DNA from 1-7 mL of cultured bacterial cells. I-Blue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. Typical yields are 20-35 µg for high-copy number plasmid or 3-10 µg for low-copy number plasmid from 4 mL of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 15 minutes. The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions.

Advantages

  • Sample: 1-7 mL of cultured bacterial cells
  • Yield: Up to 50 µg of pure plasmid DNA
  • Format: Plasmid spin column
  • Operation Time: Within 15 minutes
  • Elution Volume: 30-100 µL
  • Kit Storage: Dry at room temperature (15-25°C) for up to 1 year; PD1 and RNase A mixture should be stored at 2-8°C for up to 6 months

Quality Control
The quality of the I-Blue MINI Plasmid Kit is tested on a lot-to-lot basis by isolating plasmid DNA from a 4 mL overnight E. coli (DH5α) culture containing plasmid pBluescript (A600 > 2 U/mL). Following the purification process, a yield of more than 20 µg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid DNA (1 µg) is used in EcoRI digestion, and analyzed by electrophoresis.

Item#:
IB47180
Your Price:
43.96
Each
25 Preps

The I-Blue MINI Plasmid Kit was designed for rapid isolation of plasmid DNA from 1-7 mL of cultured bacterial cells. I-Blue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. Typical yields are 20-35 µg for high-copy number plasmid or 3-10 µg for low-copy number plasmid from 4 mL of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 15 minutes. The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions.

Advantages

  • Sample: 1-7 mL of cultured bacterial cells
  • Yield: Up to 50 µg of pure plasmid DNA
  • Format: Plasmid spin column
  • Operation Time: Within 15 minutes
  • Elution Volume: 30-100 µL
  • Kit Storage: Dry at room temperature (15-25°C) for up to 1 year; PD1 and RNase A mixture should be stored at 2-8°C for up to 6 months

Quality Control
The quality of the I-Blue MINI Plasmid Kit is tested on a lot-to-lot basis by isolating plasmid DNA from a 4 mL overnight E. coli (DH5α) culture containing plasmid pBluescript (A600 > 2 U/mL). Following the purification process, a yield of more than 20 µg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid DNA (1 µg) is used in EcoRI digestion, and analyzed by electrophoresis.

Item#:
IB47181
Your Price:
336.37
Each

PCR DNA Clean-up Kit


The 96-well PCR Clean-Up/DNA Extraction Kits provide a high-throughput, rapid and economical method to purify DNA fragments. Chaotropic salt is used to denature enzymes and in this condition, DNA fragments are bound by the glass fiber matrix in each well of the plate. Once the contaminants have been removed, the purified DNA is eluted by a low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides are effectively removed from reaction mixtures without toxic phenol extraction or alcohol precipitation. This entire protocol can be completed in 30-40 minutes, and the eluted DNA is ready to use in restricted digestion, ligation, PCR, and sequencing reactions.
Item#:
ASPCRCLEANKITB

PCR DNA Clean-up Kit


The 96-well PCR Clean-Up/DNA Extraction Kits provide a high-throughput, rapid and economical method to purify DNA fragments. Chaotropic salt is used to denature enzymes and in this condition, DNA fragments are bound by the glass fiber matrix in each well of the plate. Once the contaminants have been removed, the purified DNA is eluted by a low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides are effectively removed from reaction mixtures without toxic phenol extraction or alcohol precipitation. This entire protocol can be completed in 30-40 minutes, and the eluted DNA is ready to use in restricted digestion, ligation, PCR, and sequencing reactions.
Item#:
ASPCRCLEANKITA
  • Optimized for use with all of IBI 96-well kits
  • The thin upper portion of the manifold is designed to reduce cross contamination
  • Allows for the most effective extraction and purification of plasmid DNA, genomic DNA, viral DNA & RNA, total RNA and PCR products
  • Materials: Manifold - made of anodized aluminum, Gasket - Ethylene propylene, O-Ring - Silicone
  • Dimensions: 17x12x8cm
  • Maximum vacuum: Approx. 71cm Hg (28 in Hg) (-13.7psi)
Item#:
IB47500
Your Price:
2411.10
Each

IBI MIDI I-Blue Plasmid Sample 

The I-Blue MINI Plasmid Kit was designed for rapid isolation of plasmid DNA from 1-7 mL of cultured bacterial cells. I-Blue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. Typical yields are 20-35 µg for high-copy number plasmid or 3-10 µg for low-copy number plasmid from 4 mL of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 15 minutes. The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions.

Advantages

  • Sample: 1-7 mL of cultured bacterial cells
  • Yield: Up to 50 µg of pure plasmid DNA
  • Format: Plasmid spin column
  • Operation Time: Within 15 minutes
  • Elution Volume: 30-100 µL
  • Kit Storage: Dry at room temperature (15-25°C) for up to 1 year; PD1 and RNase A mixture should be stored at 2-8°C for up to 6 months

Quality Control
The quality of the I-Blue MINI Plasmid Kit is tested on a lot-to-lot basis by isolating plasmid DNA from a 4 mL overnight E. coli (DH5α) culture containing plasmid pBluescript (A600 > 2 U/mL). Following the purification process, a yield of more than 20 µg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid DNA (1 µg) is used in EcoRI digestion, and analyzed by electrophoresis.

Item#:
ASIBISAMPLEBLUE

The RNase-Free DNase I set ensures complete DNA removal from RNA extracted using IBI's RNA extraction kits for use in DNA sensitive downstream applications. This is an optional treatment as IBI's spin column technology yields RNA with the majority of DNA removed. Without using DNase treatment the extracted RNA can be used in downstream applications which are not DNA sensitive. This set can be used for both in column DNase digestion and DNase digestion in solution.

Item#:
ASDNARNAKIT2

Sample Types:

Blood, cultured cells, body fluids, and other animal cells

Sample Size:

5 x 10 cultured animal cells, 1 x 10 bacterial cells, or 300µl of blood
Format:
Spin columns
Operation:
Centrifuge/Vacuum

Binding Capacity:
Up to 60µg
Time:
30 minutes
Applications:

RT-PCR, Real-Time PCR, Northern Blotting, Primer Extension, RNAse Protection Assay, mRNA Selection, cDNA Synthesis
Item#:
ASDNARNAKIT1

Advantages

  • Sample: Tissue, rodent tails, ear punches, fresh or frozen blood, serum, plasma, buffy coat, body fluids, cultured cells, amniotic fluid, FFPE, hair, insects
  • Yield: Up to 5 µg of gDNA from fresh whole blood samples
  • Format: genomic DNA spin column
  • Operation Time: Within 20 minutes
  • Elution Volume: 30-100 µL
  • Kit Storage: Dry at room temperature (15-25°C) for up to 1 year

Introduction
The gMax Mini Kit is optimized for genomic, mitochondrial and virus DNA purification from whole blood (fresh blood and frozen blood), tissue, formalin-fixed paraffin-embedded tissue (FFPE), amniotic fluid and insects in one convenient kit. This DNA extraction kit uses Proteinase K and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed within 20 minutes without phenol/chloroform extraction or alcohol precipitation. The purified DNA (approximately 20-30 kb) is suitable for use in PCR or other enzymatic reactions.

Quality Control
The quality of the gMax Mini Kit is tested on a lot-to-lot basis by isolating genomic DNA from 200 μL of whole human blood. The purified DNA (5 μg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.

Kit Components


Component

IB47280

IB47281

IB47282

GST Buffer

3 mL

30 mL

75 mL

GSB Buffer

4 mL

40 mL

75 mL

W1 Buffer

2 mL

45 mL

130 mL

Wash Buffer*
(Add Ethanol)

1 mL
(4 mL)

25 mL
(100 mL)

50 mL
(200 mL)

Proteinase K**
(Add ddH2O)

1 mg
(0.10 mL)

11 mg x 2
(1.10 mL)

65 mg
(6.50 mL)

Elution Buffer

1 mL

30 mL

75 mL

GD Columns

4

100

300

2 mL Collection Tubes

8

200

600

*Add absolute ethanol (see the bottle label for volume) to Wash Buffer then mix by shaking for a few seconds. Check the box on the bottle. Be sure and close the bottle tightly after each use to avoid ethanol evaporation.

**Add ddH2O to Proteinase K (see the bottle label for volume) then vortex to ensure Proteinase K is completely dissolved. Check the box on the bottle. Once it is dissolved completely, centrifuge for a few seconds to spin the mixture down. For extended periods, the ddH2O and Proteinase K mixture should be stored at 4°C.

Item#:
ASDNARNAKIT4
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