PR1MA™ Guanidine Thiocyanate

CAS Number: 593-84-0
Molecular Weight: 118.2
Chemical Formula: CH₅N₃ • HSCN
Solubility: Water
Storage Temp: Room Temperature

Protein denaturant used for purifying proteins and nucleic acids.

Toxicity: LD50

Research or further manufacturing use only, not for food or drug use.

• Appearance: White to slightly off white crystalline powder
• Assay (Argentometric Titration): ≥ 99.0 %
• Assay (Ion Chromatography): ≥ 99.5 %
• Chloride Content: ≤ 1.0 %
• Cobalt (Co) : ≤ 5 ppm
• FTIR: Conforms to reference
• Iron: ≤ 5 ppm
• pH: 5.0 - 7.0
• DNase/ RNase: None Detected
• Melting Point: 118 - 121 ^oC
• Solubility: Pass
• Sulfate Ash: ≤ 0.5 %
• Moisture : ≤ 0.5 %
• Grade: Ultra Pure
• Country of Origin: Report

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PR1MA™ Guanidine Hydrochloride

CAS Number: 50-01-1
Molecular Weight: 95.5
Chemical Formula: CH₅N₃-HCl
Solubility: Water
Storage Temp: Room Temperature

Protein denaturant used for purifying proteins and nucleic acids.

Research or further manufacturing use only, not for food or drug use.

• Appearance: White Crystalline Powder
• Assay (Titration, dried basis): ≥ 99.5 %
• Copper (Cu): ≤ 1 ppm
• DNase, RNase, protease: Not Detected
• Free Acid: ≤ 0.01 %
• Heavy Metals (as Pb): ≤ 0.5 ppm
• Identification (IR): Conforms to Known Structure
• Iron (Fe): ≤ 1 ppm
• Loss on Drying: ≤ 1 %
• Melting Point: 185 - 188 ^oC
• pH (10% solution in H₂O @ 25^oC): 5.0 - 7.0
• Residue on Ignition: ≤ 0.05 %
• Solubility (6% Solution in H₂O): Clear
• UVA (230nm, 6M H₂O solution): ≤ 0.14
• UVA (240nm, 6M H₂O solution): ≤ 0.05
• UVA (260nm, 6M H₂O solution): ≤ 0.03
• Zinc (Zn): ≤ 1 ppm

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PR1MA™ Proteinase K

CAS Number: 39450-01-6
Solubility: Water
Storage Temp: -20°C

From Tritirachium album. Serine protease for proteolytic inactivation of nucleases during isolation of DNA and RNA.

Shipping Weight: 0 lbs
Shipping Dimensions: 1.25 x 1.75 x 1.25

Specifications

Appearance: White to Off White Lyophilized Solid
Solubility: 50mM Tris-HCl (pH 7.5), 3mM CaCl2, 50% Glycerol
Purity: ≥ 98.0 %
DNase/ RNase: Not Detected
Activity: ≥ 30.0 U/mg
Unit Definition: 1 unit is defined as the amount of enzyme that will liberate 1.0 µmol of tyrosine from casein/min @37°C, pH 7.5
Country of Origin: China

Research or further manufacturing use only, not for food or drug use.

Proudly made in the USA! Click here for more made in America products!
  

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PR1MA™ Formamide, Super Pure

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PR1MA™ Sodium Acetate, Anhydrous

CAS Number: 127-09-3
Molecular Weight: 82.0
Chemical Formula: C₂H₃O₂Na
Solubility: Water
Storage Temp: Room Temperature

Research or further manufacturing use only, not for food or drug use.

• Appearance: White Powder
• Assay: ≥ 99.0 %
• pH (5% Solution): 7.0 - 9.2
• Solubility: Soluble in Water
• Loss on Drying (120 ^oC): ≤ 1.0 %
• Calcium (Ca): ≤ 0.005 %
• Chloride (Cl): ≤ 0.002 %
• Heavy Metals (as Pb): ≤ 0.001 %
• Insoluble Matter: ≤ 0.01 %
• Iron (Fe): ≤ 0.001 %
• Magnesium (Mg): ≤ 0.002 %
• Phosphate: ≤ 0.001 %
• Sulfate (SO₄): ≤ 0.003 %
• Grade: Meet USP/ACS Specifications

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PR1MA™ Imidazole [Glyoxaline] [1,3-Diaza-2-,4-cyclopentadiene]

Common Name: [Glyoxaline] [1,3-Diaza-2,4-cyclopentadiene]
CAS Number: 288-32-4
Molecular Weight: 68.1
Chemical Formula: C₃H₄N₂
Solubility: Water
Storage Temp: Room Temperature

pKa (25°C): 6.9-7.0
pH range: 6.2-7.8

Research or further manufacturing use only, not for food or drug use.

• Appearance: White to off white crystals
• Assay: ≥ 99.0 %
• DNase: Not Detected
• Identification: Pass Test
• Loss on Drying: ≤ 0.5 %
• Melting Point: 88 - 92 ^oC
• OPTICAL @ 260 NM: ≤ 0.2
• PKa @ 25°C: 6.79 - 7.19
• Protease: Not Detected
• RNase: Not Detected

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PR1MA™ Glycerol

CAS Number: 56-81-5
Molecular Weight: 92.1
Chemical Formula: C₃H₈O₃
Solubility: Water
Storage Temp: Room Temperature

Used in sample preparation and gel formation for polyacrylamide gel electrophoresis. Also used in casting gradient gels, as a protein stabilizer and storage buffer component.

Research or further manufacturing use only, not for food or drug use.

• Appearance: Clear and Colorless Liquid
• Chloride (Cl): ≤ 0.001 %
• Chlorinated Compounds: ≤ 0.003 %
• Color, APHA: ≤ 10.0
• Fatty Acids & Esters (USP): ≤ 1.0 (ml 0.5 N NaOH) %
• Glycerine: ≥ 99.7 %
• Heavy Metals: ≤ 5.0 ppm
• Identification C (GC): Pass
• Limit of Diethylene Glycol & RC: Pass
• Purity (USP): 99.0 - 101.0 %
• Residue on Ignition: ≤ 0.01 %
• Specific Gravity: ≥ 1.259
• Water: ≤ 5.0 %
• Date of Manufacture: Report

Radiance ECL Chemiluminescent Substrate

Chemiluminescent HRP substrate for chemiluminescent Western blots

Radiance ECL is an HRP substrate with long-lasting signal and sensitivity down to low picograms. Use up to 10 times less antibody than other substrates used in life science.

Size: 500 mL

Features

• Performance — compare performance to SuperSignal West Pico and Amersham ECL
• Sensitivity — high sensitivity in low picograms
• Duration — Signal duration 6-8 hours
• Chemiluminescent Western Blotting
• The most common method for Western blot detection is chemiluminescence. Detection by chemiluminescence is relatively sensitive and works well for detecting individual or low abundance proteins.   

Radiance Plus Chemiluminescent Substrate

The most sensitive HRP substrate available for chemiluminescent Western blotting.

With attomole sensitivity and a long-lasting signal, Radiance Plus allows you to detect bands not visualized with other substrates. High signal-to-noise and a large dynamic range make it ideal for quantifying low-intensity bands.

Size: 150 mL

Features

• Low-abundance protein detection
• Compare performance to competitor products, like SuperSignal West Femto and Amersham ECL Select
• Sensitivity in low femtograms
• Signal duration 6-8 hours   

Radiance Q Chemiluminescent Substrate

Radiance Q chemiluminescent substrate is specially formulated for use with Azure Imaging Systems, or other CCD imagers. This novel horseradish peroxidase (HRP) substrate provides a strong, long-lasting signal, the broadest useful linear range, and high sensitivity for the most quantitative chemiluminescent Western assays.

Size: 150 mL

Features
 

Increase the sensitivity and quantitative accuracy of your chemiluminescent Western blots. Specially developed for CCD imaging, Radiance Q produces a strong, long-lasting signal with extremely low background, perfect for detecting low abundance proteins. Because the substrate is not depleted at high protein loads, Radiance provides the largest dynamic range of any chemiluminescent substrate, improving quantitative accuracy.

• Extended dynamic range for detecting low and high abundance proteins within the same blot
• Compare performance to SuperSignal West Dura and Amersham ECL Prime
• Sensitivity in mid femtograms
• Signal duration 10-24 hours   

Electrophoresis Buffers
QuickSilver - TAE (Tris-acetate-EDTA)

TAE is ideal for separation of large DNA fragments in agarose gels and is used as running buffer and in gel preparation.

TAE is the buffer of choice when recovering DNA from gels and when downstream processing involves enzymatic reactions. Final concentration of a 1X working solution is 40mM Tris, 20mM acetic acid, 1mM EDTA.

QS1000 QuickSilver TAE Buffer, 1X, 100 packs

QuickSilver - TBE (Tris-borate-EDTA)

TBE is used in gel preparation and as a running buffer for electrophoresis of nucleic acids. TBE works best for resolution of smaller fragments (<2kb). Its higher buffering capacity makes it useful for longer runs. The final concentration of a 1X working solution is 89mM Tris, 89mM boric acid, 2mM EDTA.

QS1001 QuickSilver TBE Buffer, 1X, 50 packs

QuickSilver - Fast Running Buffer

QuickSilver Fast Running Buffer is a proprietary blend formulated for agarose gel electrophoresis at high voltage (300-350V). Its strong buffering capacity minimizes heat generation all allows for extremely fast separation of fragments smaller than 5kb. A typical 30-minute run can be performed in just 10 minutes using this buffer.

QS1002 QuickSilver Fast Running Buffer, 1X, 100 packs
 
 

Protein Electrophoresis Buffers

QuickSilver™ TGS (Tris-Glycine-SDS)

Tris-Glycine-SDS is one of the most common running buffers used for SDS-PAGE. A 1X solution contains 25mM Tris, 192mM Glycine and 0.1% SDS.

QS1100 QuickSilver Tris Glycine-SDS Buffer, 1X, 50 packs

QuickSilver™ Tris-MES-SDS

Small to medium molecular weight proteins can be resolved on SDS-PAGE gels using Tris-MES-SDS as a running buffer. Proteins resolve faster in gels using Tris-MES-SDS as a running buffer than those using Tris-MOPS-SDS. A 1X solution has a final concentration of 50mM Tris, 50mM MES, 0.1% SDS and 1mM EDTA.

QS1101 QuickSilver Tris-MES-SDS Buffer, 1X, 50 packs

QuickSilver™ Tris-MOPS-SDS

Tris-MOPS-SDS is ideal for use as a running buffer when resolving medium to large proteins with SDS-PAGE. Gels run with Tris-MOPS-SDS buffer run slower than those using Tris-MES-SDS buffer. The final concentration of a 1X working solution is 50mM Tris, 50mM MOPS, 0.1% SDS and 1mM EDTA.

QS1102 QuickSilver Tris-MOPS-SDS, 1X, 50 packs

Western Blotting, ELISA, Cell Culture, Chromatography

QuickSilver™ PBS and PBST

Because its osmolality and ion concentration closely mimic that of the human body, PBS is commonly used for cell culture applications, as well as HPLC and MS. PBST is a wash buffer and diluent for ELISA. The 1X PBS solution contains 137mN NaCl, 2.7mM KCl and 10mM phosphate. PBST is supplemented with 0.05% Tween-20.

QS1200 QuickSilver PBS Buffer, 1X, 100 packs

QS1201 QuickSilver PBST Buffer, 1X, 100 packs

QuickSilver™ TBS and TBST

TBS and TBST are used to maintain pH within a very narrow range for a variety of applications including western blotting and immunoassays. The final concentration of a 1X working solution is 137mM NaCl, 2.7mM KCl, 50mM Tris. In addition, 1X TBST contains 0.1% Tween-20.

QS1202 QuickSilver TBS buffer, 1X, 100 packs

QS1203 QuickSilver TBST buffer, 1X, 100 packs

Guanidine Hydrochloride

• Appearance: White Crystalline Powder
• Purity: ≥ 99.0%
• Solubility (6M Aqueous): Clear and Colorless
• pH: 5.0 - 7.0
• Identity (FTIR): Conforms to Structure
• Water: ≤ 0.3%
• Heavy Metals (as Fe): ≤ 5 ppm
• A260 (6M Aqueous): ≤ 0.10
• A280 (6M Aqueous) ≤ 0.05
• RNase: None Detected
• DNase: None Detected

*Custom sizes available upon request.

DOT Information: Non-regulated.