Hoefer® Standard and Preparative Combs for Mini Vertical Systems

 

Compatible with SE300 MiniVE, SE260, and the SE250 Series Vertical Electrophoresis Systems.

 

Precision machined from Teflon®, which is highly resistant to acids, bases, and all commonly used buffer systems. An important benefit of Teflon® combs, compared to other types, is that they are easily removed while maintaining gel integrity. They are lightweight and virtually unbreakable when used with reasonable care. Some combs have microtiter spacing for use with multi-channel pipettes. (See Specifications.)

 

Standard Combs come attached to a standard back, which produces maximum well depths of 25 mm, except for the 28-well combs, which produce well depths of 15 mm.

• 9- and 18-well Standard Combs have microtiter spacing for use with multichannel pipettes.
• Preparative Combs have adjustable comb backs (10, 15, and 25 mm depth) and form 1 large preparative well plus 1 or 2 small reference wells.

Specifications
 

Item #	Type	Wells	Teeth	Thickness	Width	Depth
SE211A-5-75	Standard Comb	5 Wells	5 Teeth	0.75 mm	13 mm	9.8 mm
SE211A-5-10	Standard Comb	5 Wells	5 Teeth	1 mm	13 mm	13 mm
SE211A-5-15	Standard Comb	5 Wells	5 Teeth	1.5 mm	13 mm	19.5 mm
SE211A-9-10	Standard Comb, w/ Microtiter Spacing	9 Wells	9 Teeth	1 mm	5.8 mm	5.8 mm
SE211A-10-75	Standard Comb	10 Wells	10 Teeth	0.75 mm	4.8 mm	3.6 mm
SE211A-10-10	Standard Comb	10 Wells	10 Teeth	1 mm	4.8 mm	4.8 mm
SE211A-10-15	Standard Comb	10 Wells	10 Teeth	1.5 mm	4.8 mm	7.2 mm
SE211A-12-10	Standard Comb	12 Wells	12 Teeth	1 mm	4.75 mm	4.75 mm
SE211A-15-75	Standard Comb	15 Wells	15 Teeth	0.75 mm	2.9 mm	2.2 mm
SE211A-15-10	Standard Comb	15 Wells	15 Teeth	1 mm	2.9 mm	2.9 mm
SE211A-15-15	Standard Comb	15 Wells	15 Teeth	1.5 mm	2.9 mm	4.4 mm
SE211A-18-10	Standard Comb, w/ Microtiter Spacing	18 Wells	18 Teeth	1 mm	2.9 mm	2.9 mm
SE211A-R-75	Preparative Comb	1/1 Wells	1/1 Teeth	0.75 mm	68/5 mm	51/3.8 mm
SE211A-R-10	Preparative Comb	1/1 Wells	1/1 Teeth	1 mm	68/5 mm	68/5 mm
SE211A-R-15	Preparative Comb	1/1 Wells	1/1 Teeth	1.5 mm	68/5 mm	120/7.5 mm

Hoefer® Combs for SUB25 Submarine Gel Electrophoresis Systems

 

Available in multiple tooth configurations and thicknesses to suit various sample volumes and well sizes. Hoefer® combs ensure sharp, consistent, well formation for accurate, reproducible results.

• MC = Microtiter spacing for use with a multichannel pipette
• Sample volume listed is for a 5 mm deep well
• For use with SUB25 Submarine Gel Electrophoresis Systems

Specifications

Item #	Description	Wells	Teeth	Thickness	Width	Sample Vol.
SUB25-C1-26MC	SUB25 Comb	26 Wells	26 Teeth	1 mm	7 mm	30 uL
SUB25-C15-26MC	SUB25 Comb	26 Wells	26 Teeth	1.5 mm	7 mm	45 uL
SUB25-C2-26MC	SUB25 Comb	26 Wells	26 Teeth	2 mm	7 mm	60 uL
SUB25-C1-52MC	SUB25 Comb	52 Wells	52 Teeth	1 mm	3 mm	13 uL
SUB25-C15-52MC	SUB25 Comb	52 Wells	52 Teeth	1.5 mm	3 mm	20 uL
SUB25-C2-52MC	SUB25 Comb	52 Wells	52 Teeth	2 mm	3 mm	25 uL

 

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Hoefer® Standard Format Glass Plates for Vertical Systems

 

Standard format glass plates are for SE660, SE600X Chroma, SE600, SE640, SE410, and SE400 Vertical Gel Electrophoresis Units.

 

Run as many as four 0.75, 1.0, or 1.5 mm thick gels on SE600 Series units and two gels on the SE400 Series at one time under identical conditions using the double gel capacity Divider Plates. Insert a divider plate between the glass plates that make up a single gel sandwich. Place spacers on each side of the divider plate to form two gel cavities for pouring gels. This two gel “club sandwich” fits into the same casting stand and seals perfectly.

 

Specifications
 

Item #	Description	Plate Size	Qty
SE6402	Regular Glass Plates	7.09 x 3.15 In. | 18 x 8 cm	2/pk
SE6402LF	Low Fluorescence Glass Plates	7.09 x 3.15 In. | 18 x 8 cm	2/pk
SE6402D	Notched Glass Divider Plate	7.09 x 3.15 In. | 18 x 8 cm	1/ea
SE6102	Regular Glass Plates	7.09 x 6.30 In. | 18 x 16 cm	2/pk
SE6102LF	Low Fluorescence Glass Plates	7.09 x 6.30 In. | 18 x 16 cm	2/pk
SE6102D	Notched Glass Divider Plate	7.09 x 6.30 In. | 18 x 16 cm	1/ea
SE6602	Regular Glass Plates	7.09 x 9.45 In. | 18 x 24 cm	2/pk
SE6602LF	Low Fluorescence Glass Plates	7.09 x 9.45 In. | 18 x 24 cm	2/pk
SE6602D	Notched Glass Divider Plate	7.09 x 9.45 In. | 18 x 24 cm	1/ea

Hoefer® Glass and Alumina Plates for Mini Vertical Systems

 

Small Glass and Alumina Plates for Mini Vertical Units.

Compatible with SE300 MiniVE, SE260, and the SE250 Series Vertical Electrophoresis Systems. (see notes below). 

This set includes precision-crafted glass and alumina plates for electrophoresis applications. Includes a variety of notched and rectangular plates in 10 x 8 cm and 10 x 10.5 cm sizes, ideal for customizing your vertical gel setup.

*Alumina plates are not recommended for use with the Hoefer® miniVE™ Vertical Protein Electrophoresis and Blotting System. 
 

Specifications
 

Item#	Description	Plate Size	Weight	Qty
SE202P-10	Rectangular Glass Plates	3.94 x 3.15 In. | 10 x 8 cm	0.66 lb | 0.3 kg	10/pk
SE202GN-5	Notched Glass Plates	3.94 x 3.15 In. | 10 x 8 cm	0.44 lb | 0.2 kg	5/pk
SE262P-5	Rectangular Glass Plates	3.94 x 4.13 In. | 10 x 10.5 cm	0.66 lb | 0.3 kg	5/pk
SE262GN-5	Notched Glass Plates	3.94 x 4.13 In. | 10 x 10.5 cm	0.66 lb | 0.3 kg	5/pk
SE202N	Notched Alumina Plate	3.94 x 3.15 In. | 10 x 8 cm	0.22 lb | 0.1 kg	1/ea
SE202N-10	Notched Alumina Plates	3.94 x 3.15 In. | 10 x 8 cm	0.44 lb | 0.2 kg	10/pk
SE262N-5	Notched Alumina Plates	3.94 x 4.13 In. | 10 x 10.5 cm	0.44 lb | 0.2 kg	5/pk

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Hoefer® Spacers for Dual and Air Cooled Vertical Systems

 

Gel Spacers for SE400 and SE600 Series Vertical Electrophoresis Systems.

 

Engineered for consistent gel casting, these precision-machined spacers are made from high-strength, chemically resistant plastics. Available in three lengths (8, 16, and 24 cm) and multiple thicknesses (0.75 mm, 1.0 mm, 1.5 mm) to accommodate various gel formats.

All 1.0 mm spacers are white for quick identification during setup.

 

Specifications
 

Item #	Thickness	Length	Width
SE6419-2-75	0.030 In. | 0.75 mm	3.15 In. | 8 cm	0.79 In. | 2 cm
SE6419-2-10	0.039 In. | 1 mm	3.15 In. | 8 cm	0.79 In. | 2 cm
SE6419-2-15	0.059 In. | 1.5 mm	3.15 In. | 8 cm	0.79 In. | 2 cm
SE6119-2-75	0.030 In. | 0.75 mm	6.30 In. | 16 cm	0.79 In. | 2 cm
SE6119-2-10	0.039 In. | 1 mm	6.30 In. | 16 cm	0.79 In. | 2 cm
SE6119-2-15	0.059 In. | 1.5 mm	6.30 In. | 16 cm	0.79 In. | 2 cm
SE6619-2-75	0.030 In. | 0.75 mm	9.45 In. | 24 cm	0.79 In. | 2 cm
SE6619-2-10	0.039 In. | 1 mm	9.45 In. | 24 cm	0.79 In. | 2 cm
SE6619-2-15	0.059 In. | 1.5 mm	9.45 In. | 24 cm	0.79 In. | 2 cm
SE6118-2-10	0.039 In. | 1 mm	6.30 In. | 16 cm	0.39 In. | 1 cm
SE6118-2-15	0.059 In. | 1.5 mm	6.30 In. | 16 cm	0.39 In. | 1 cm

Hoefer® T-Spacers for Mini Vertical Systems

 

Compatible with SE300 MiniVE, SE260, and the SE250 Series Vertical Electrophoresis Systems.

 

Ensure accurate gel thickness and leak-free casting with precision-milled T-spacers. All spacers are precision-machined from durable plastics for high chemical resistance and optimum tensile strength. These spacers provide consistent alignment and long-term durability for mini vertical systems.

 

Available in multiple lengths (8 cm and 10.5 cm) and thicknesses (0.75 mm, 1.0 mm, and 1.5 mm) to suit your specific gel requirements.

 

Specifications
 

Item #	Thickness	Length	Width
SE2119T-2-75	0.030 In. | 0.75 mm	3.15 In. | 8 cm	0.39 In. | 1 cm
SE2119T-2-10	0.039 In. | 1 mm	3.15 In. | 8 cm	0.39 In. | 1 cm
SE2119T-2-15	0.059 In. | 1.5 mm	3.15 In. | 8 cm	0.39 In. | 1 cm
SE2619T-2-75	0.03 In. | 0.75 mm	4.13 In. | 10.5 cm	0.39 In. | 1 cm
SE2619T-2-10	0.039 In. | 1 mm	4.13 In. | 10.5 cm	0.39 In. | 1 cm
SE2619T-2-15	0.059 In. | 1.5 mm	4.13 In. | 10.5 cm	0.39 In. | 1 cm

Multi-colored 96 Well Orienter

 

For easy identification of samples in microplates, vertically or horizontally oriented bright colors make it easy to distinguish between columns. Fits most standard flat and round bottom 96-well plates. Available in horizontal and vertical orientations.
 

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Glow-Writer Pen

Glow Writeris a fine-point phosphorescent ink marking pen for making experimental notations and other markings on chemiluminescent and radioisotope-labeled substrate materials (e.g., transfer membranes, dried gels, genomic and proteomic matrices, etc.) being analyzed by X-ray film autography or electronic imaging. The unique marker delivers a phosphor-containing ink through a fine point porous nib. Before exposure to X-ray film, the phosphorescent writing is excited by room light. A phosphor ink having an optimum concentration of phosphor and a relatively short afterglow halflife (approximately 2-3 minutes) has been selected so that upon exposure to X-ray film,autographic exposures of almost any duration will be easily readable on the film. The fine point nib is easily replaceable if it dries out.

Fine-Point Phosphorescent Marking Pen for Laboratory Autography

• Fine Point Nib- Provides fine writing and greater information density
• Specially Designed- Ideal glow intensity and afterglow duration helps prevent over-andunder exposure
• No Ink Clogs- Low ink viscosity and porous nib prevents ink from clogging

Aluminum Autoradiography Cassette 5 x 7

Classic Push-Button Cassettes

• Constructed From Lightweight Aluminum & Nylon
• Special Curved Door Gives Exceptional Screen-Film Contact

RunBlue gels are more rigid and up to 10 times stronger than conventional precast gels resulting in more robust gels that can be handled with confidence. No tearing - an 8% gel can be thrown into the air and caught without breaking!

• RunBlue gels are manufactured using a unique method of polymerisation that results in more uniform and more complete polymerisation of the Acrylamide when compared to conventional, chemical based methods. This results in gels that have superior batch to batch reproducibility and practically eliminates exposure to un-polymerised Acrylamide.
• RunBlue gels incorporate a stacking gel that has been dyed (red for SDS) for the simplest ever sample loading. In addition, there is no comb to remove as the top of the teeth are fixed in place. This not only prevents the teeth from being accidentally torn off during comb removal but that all of the wells are uniform, with the teeth remaining vertical. This prevents sample cross contamination / inability to load desired Vol. The teeth also protrude above the lower front plate of the gel cassette preventing sample flow over into adjacent wells.
• Runblue gels are available in two different cassette sizes providing compatibility with the most common gel tanks and apparatus.  10x10 cm cassettes are compatible with Invitrogen/Life Technologies equipment and any other equipment designed for this format, such as the Hoeffer and Owl tanks. Our 8x10 cm cassette is designed to fit the Biorad equipment (Mini Protean I, II and II, Tetra etc).
• The resolving gel on RunBlue gels extends to the very bottom of the cassette which not only provides improved resolution but also there is no protruding gel that requires trimming prior to blotting. There is also no additional tape / covers to remove - simply take the gel from the pack and use.
• RunBlue gels are supplied in boxes of 10 gels. They are available in a 12 well format, covering a range of concentrations and gradients.

Benefits of RunBlue Precast Gels

• 2 year shelf life - never throw away another gel
• Up to 10x stronger - no more torn, ruined gels
• Load up to 20µl - see everything in your sample
• Dyed stacking gel - makes gel loading child's play (SDS = Red)
• No comb to remove - no more broken or bent teeth
• Full length resolving gel - improved resolution and better blotting
• 10x10 cm and 8x10 cm cassette - total compatibility with most popular tanks including Invitrogen and Biorad equipment
• Fast delivery with immediate and expert technical support for total peace of mind
• Best value gels available - save money!

MAXI Flex Tube Kit

MAXI Flex Tubes combine two modes of action: electro-elution of nucleic acid molecules from polyacrylamide or agarose gels and dialysis or buffer exchange of protein molecules. Flex Tubes allow rapid, secure, simple loading and recovery, with high performance as the most convenient, user friendly, electro-elution and dialysis system on the market.

KIT CONTENTS

• Flex Tubes 2/10/30/50/ 100 pieces
• Supporting tray (for electro elution protocol) 1ea. (select kits)
• Floating rack (for dialysis protocol) 1ea. (select kits)
• Information and Protocol Manual 1ea.

SPECIFICATIONS

• Membrane cut-off: 3.5K(11bp), 6-8K(18-24bp), 12-14K(36-42bp), 25K(76bp) or 50K(152bp) MWCO
• Tube volume: 3ml
• Dialysis volume: 0.1-3ml
• Min. sample size for extraction: 20µg
• Max. gel slice: 2cm x 1cm
• Membrane ultra-clean, sulfur and heavy metal free. EDTA treated
• Flex Tube MWCO are in kilo Daltons (K) for proteins and corresponding base pairs (bp) for nucleic acids as indicated in the table below:

kilo Daltons	base pairs
1K	3 bp
3.5K	11bp
6-8K	18-24bp
12-14K	36-42bp
25K	76bp
50K	152bp

 

APPLICATIONS

• Dialysis, electro-elution or buffer exchange with volumes between 0.1-3ml
• Preparation of protein samples for MALDI-MS
• Sample concentration
• Large-scale protein dialysis, such as antibodies and recombinant protein purification
• Removal of contaminating micro-molecules
• Tissue culture extraction purification
• Removal of salts, surfactants, solvents, and detergents
• Complex formation studies (protein-protein, protein-DNA, and protein-RNA)
• pH and buffer adjustment of sample solutions, protein extraction or cell extraction
• High throughput dialysis
• Peptide dialysis, as small as 10 amino acids
• Virus-particles purification

MIDI Flex Tube Kit

MIDI Flex Tubes combine two modes of action: electro-elution of nucleic acid molecules from polyacrylamide or agarose gels and dialysis or buffer exchange of protein molecules. Flex Tubes allow rapid, secure, simple loading and recovery, with high performance as the most convenient, user friendly, electro-elution and dialysis system on the market.

KIT CONTENTS

• Flex Tubes 2/10/30/50/ 100 pieces
• Supporting tray (for electro elution protocol) 1ea. (select kits)
• Floating rack (for dialysis protocol) 1ea. (select kits)
• Information and Protocol Manual 1ea.

SPECIFICATIONS

• Membrane cut-off: 1K(3bp), 3.5K(11bp) or 6-8K(18-24bp) MWCO
• Tube volume: 800µl
• Dialysis volume: 50-800µl
• Min. sample size for extraction: 0.5µg
• Max. gel slice: 1cm x 0.5cm
• Membrane ultra-clean, sulfur and heavy metal free. EDTA treated
• Flex Tube MWCO are in kilo Daltons (K) for proteins and corresponding base pairs (bp) for nucleic acids as indicated in the table below:

kilo Daltons	base pairs
1K	3 bp
3.5K	11bp
6-8K	18-24bp
12-14K	36-42bp
25K	76bp
50K	152bp

 

APPLICATIONS

• Dialysis, electro-elution or buffer exchange with volumes between 50-800µl
• Preparation of protein samples for MALDI-MS
• Sample concentration
• Large-scale protein dialysis, such as antibodies and recombinant protein purification
• Removal of contaminating micro-molecules
• Tissue culture extraction purification
• Removal of salts, surfactants, solvents, and detergents
• Complex formation studies (protein-protein, protein-DNA, and protein-RNA)
• pH and buffer adjustment of sample solutions, protein extraction or cell extraction
• High throughput dialysis
• Peptide dialysis, as small as 10 amino acids
• Virus-particles purification.