PR1MA™ DTT [DL-Dithiothreitol] (Cleland's Reagent)]

Common Name: [DL-Dithiothreitol] (Cleland's Reagent)]
CAS Number: 3483-12-3
Molecular Weight: 154.25
Chemical Formula: C₄H₁₀O₂S₂
Solubility: Water
Storage Temp: 2-8°C

Protective reagent for maintaining -SH groups in reduced state. DTT permeates the cell membrane, protecting protein sulfhydryls, thereby restoring enzyme activity lost by oxidation of the groups.

Research or further manufacturing use only, not for food or drug use.

• Appearance: A white free flowing powder, hygroscopic
• Solubility: Freely soluble in methanol, methylene chloride
• Clarity in water: 5% (w/v) solution in water is clear and colorless
• Identification by TLC: Rf value of the sample is comparable with that of the standard
• Melting Range: 37.0 - 44.0 °C
• Related Substance (UV Spectroscopy- Content of Oxidised DTT): 0.4 %
• Assay (By Iodimetry): ≥ 98.0 %
• Country of Origin: Report

PR1MA™  HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)]

Common Name: [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)]
CAS Number: 7365-45-9
Molecular Weight: 238.3
Chemical Formula: C₈H₁₈N₂O₄S
Solubility: Water
Storage Temp: Room Temperature

Zwitterionic N-substituted aminosulfonic acid buffer.

• pka (25°C): 7.5
• pH Range (Working): 6.8 - 8.2
• Heavy Metals: <0.001% Max

• Appearance: White Crystalline Powder
• Assay (Titration, dried basis): ≥ 99.5 %
• DNase, RNase, protease: Not Detected
• Endotoxin (0.2% Solution): ≤ 0.25 EU/ml
• Heavy Metals (as Pb): ≤ 0.001 %
• Identification (IR): Pass
• Iron (Fe): ≤ 0.0005 %
• Loss on Drying: ≤ 1.0 %
• pH (5% in water): 5.0 - 6.5
• pKa @ 20°C: 7.35 - 7.70
• Residue on Ignition: ≤ 0.1 %
• Solubility (1M Solution in Water ): Clear and Colorless Solution
• Sulfate: ≤ 0.05 %
• UV Absorbance (1M@260nm): ≤ 0.05
• UV Absorbance (1M@280nm): ≤ 0.05

PR1MA™ Acrylamide/bis-Acrylamide, Premixed Powder 19:1 Ratio

Storage Temp: Room Temperature

Pre-measured powder blend of acrylamide and bis-acrylamide for simple stock solution preparation.

WARNING! This product can expose you to Acrylamide, a chemical which is known to the State of California to cause cancer, developmental and reproductive harm. For more information go to www.P65Warnings.ca.gov.

Research or further manufacturing use only, not for food or drug use.

• Appearance: Powder
• Solubility: Clear and complete
• Conductivity: ≤ 10 µmhos

PR1MA™ Puromycin Dihydrochloride

CAS Number: 58-58-2
Molecular Weight: 544.43
Chemical Formula: C₂₂H₂₉N₇O₅ • 2HCl
Solubility: Water
Storage Temp: -20°C

Amino nucleoside antibiotic allows the selection of cells expressing the pac gene. Inhibits the growth of gram-positive bacteria and various animal and insect cells.

Research or further manufacturing use only, not for food or drug use.

• Appearance: White to Off White Powder
• Identification (TLC): Conforms
• Melting Point: 168 - 182 ^oC
• Purity (HPLC): ≥ 98.0 %
• Solubility: Clear, colorless or light yellow solution
• Water (KF): ≤ 12.0 %

PR1MA™ Peptone, Soy 

CAS Number: 91079-46-8
Solubility: Water
Storage Temp: Room Temperature

From papaic hydrolysis of soybean meal. Low NaCl content.

Research or further manufacturing use only, not for food or drug use.

• Amino Nitrogen: ≥ 2.2 %
• Ash: ≤ 15 %
• Loss on Drying: ≤ 6.0 %
• pH (2% water) @25°C: 6.5 - 7.5
• Total Nitrogen: ≥ 7.0 %
• Coliform: Negative
• E.Coli: Negative
• Molds and Yeasts: ≤ 100 cfu/g
• Salmonella: Negative /25g
• Standard Plate Count : ≤ 5000 cfu/g
• Broth Formulations (Growth Supporting Properties): Satisfactory
• Peptone Agar (Growth Supporting Properties): Satisfactory
• Country of Origin of Raw Material : Report
• Raw Material Species: Plant
• Country of Origin of Enzymes : Report
• Enzyme Species: Porcine
• Country Of Manufacture: Report

PR1MA™ LB Broth Powder [Lennox L Broth]

Common Name: Lennox L Broth
Solubility: Water
Storage Temp: Room Temperature

For propagation and maintenance of E. Coli. Low Salt Formula. Molecular Biology Grade.

Dissolve 20 grams in 1 liter distilled water. Then adjust pH to 7.2. Heat and frequently agitate to completely dissolve. Sterilize by autoclaving for 15 minutes.

Ingredients:

• Tryptone: 10 g/L
• Yeast Extract: 5 g/L
• Sodium Chloride: 5 g/L

Research or further manufacturing use only, not for food or drug use.

• DCM Appearance: Homogeneous free flowing, light beige
• PPM Appearance: Clear, No to light ppt, light yellow
• pH (2% water) @25°C: 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent
• Sodium Chloride: 5 g/L
• Yeast Extract: 5 g/L
• Casein Peptone: 10 g/L

PR1MA™  Blasticidin S Hydrochloride Powder

CAS Number: 3513-03-9
Molecular Weight: 458.9
Chemical Formula: C₁₇H₂₆N₈O₅ • HCl
Solubility: Water
Storage Temp: -20°C

• Appearance: White Powder
• Endotoxin : ≤ 1.0 EU / mg
• Identification: Pass Test
• Potency: ≥ 850 ug / mg
• Purity (HPLC): ≥ 98.0 %
• Solubility: Clear, Slightly yellow solution at 5 mg / mL in water

PR1MA™ Terrific Broth, Modified, Granulated

CAS Number: 91079-40-2; 8013-01-2; 7778-77-0; 7758-11-4
Solubility: Water
Storage Temp: Room Temperature

Enriched media for maintenance and propagation of E. Coli and plasmid growth. Produces higher cell densities resulting in greater yields of DNA or recombinant proteins. Glycerol is used as a carbon source with this formulation instead of glucose as it will not ferment to acetic acid.

Ingredients:

• Casein Digest Peptone: 12g/L
• Yeast Extract: 24g/L
• Dipotassium phosphate: 9.5g/L
• Monopotassium phosphate: 2.2g/L

Research or further manufacturing use only, not for food or drug use.

• DCM Appearance: Homogeneous, free flowing beige to yellow-beige
• PPM Appearance: Clear, yellow to amber no precipitate (ppt)
• pH (4.76% solution at 25°C): 6.8 - 7.4
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

PR1MA™  Tris Base Ultra Pure [Tris (Hydroxymethyl) Aminomethane]

Common Name: Tris (Hydroxymethyl) Aminomethane
CAS Number: 77-86-1
Molecular Weight: 121.1
Chemical Formula: C₄H₁₁NO₃
Solubility: Water
Storage Temp: Room Temperature

Highly purified for use in molecular biology applications.

Moisture: <0.2%
Color: <20 APHA (colorless)
Heavy Metals (as Pb): <0.0001%
Insoluble Matter: <0.005%
Heavy Metals: 5 ppm
pKa (20°C): 8.1 ± 0.1

Research or further manufacturing use only, not for food or drug use.

• Appearance: White Crystalline Powder
• Purity (Dried Basis By GC): ≥ 99.9 %
• Related Substances By GC: ≤ 0.1 %
• Water: ≤ 0.2 %
• Loss on Drying @ 105 ^oC, 3hrs: ≤ 1.0 %
• Melting Point: 170 - 172 ^oC
• APHA Color, 20% Aqueous solution: ≤ 20
• Insoluble Matter: ≤ 0.005 %
• Heavy Metals (as Pb): ≤ 5 ppm
• Residue on Ignition: ≤ 0.5 %
• Iron (Fe): ≤ 5 ppm
• UV Absorbance @260nm, 10% Aqueous solution : ≤ 0.03
• UV Absorbance @280nm, 10% Aqueous solution : ≤ 0.02
• UV Absorbance @290nm, 40% solution in H₂O: ≤ 0.20
• UV Absorbance @430nm, 10% Aqueous solution : ≤ 0.004
• Identification A: Pass
• Identification B: Pass
• Identification C: Pass
• pH (5% in water): 10.0 - 11.5
• Arsenic (As): ≤ 1 ppm
• Chloride: ≤ 100 ppm
• Copper (Cu): ≤ 1 ppm
• DNase, RNase, protease: None Detected
• Lead: ≤ 1 ppm
• Magnesium (Mg): ≤ 1 ppm
• pH (1M in H₂O): 10.5 - 11.5
• Residual Solvents: Conforms to USP/EP
• Residue on Ignition (USP): ≤ 0.1 %
• Solubility (2M In H₂O): Pass

PR1MA™  PBS 1X Solution [Phosphate Buffered Saline 1X Sterile Solution]

Common Name: Phosphate Buffered Saline 1X Sterile Solution
CAS Number: 7647-14-5, 7447-40-7, 7558-79-4, 7778-77-0, 7732-18-5
Solubility: Water
Storage Temp: Room Temperature

Phosphate Buffered Saline is a buffer solution commonly used in biological research. PBS has many uses because it is isotonic and non-toxic to most cells. These uses include substance dilution and cell container rinsing.

Ready to use sterile 1X solution.

Ingredients:

• Sodium Chloride: 137 mM
• Potassium Chloride: 2.7 mM
• Phosphate Buffer: 11.9 mM
• pH: 7.4
• Does not contain Calcium or Magnesium

Research or further manufacturing use only, not for food or drug use.

• Appearance: Clear and Colorless Solution
• pH: 7.25 - 7.55

PR1MA™  Doxycycline Hydrochloride 

• Appearance: Yellow crystalline powder
• Absorbance (349nm): 300 - 335
• Ethanol: 4.3 - 6.0 %
• Heavy Metals : ≤ 50 ppm
• Identification (A, B, C): Pass Test
• Light Absorbing Impurities (490nm): ≤ 0.5
• pH: 2.0 - 3.0
• Potency : 800 - 920 µg/mg
• Purity: 95.0 - 102.0 %
• Related Compound (6-Epidoxycyline): ≤ 2.0 %
• Related Substance (Any Other Impurity): ≤ 0.5 %
• Specific Optical Rotation: -120 - -105 ^o
• Sulfated Ash: ≤ 0.4 %
• Water: 1.4 - 2.8 %

PR1MA™  Metronidazole

• Appearance: White or yellowish powder
• Assay (Dry Basis): 99.0 - 101.0 %
• Identification: Conform
• Sulfated Ash: ≤ 0.1 %
• Non Animal Origin: Non Animal Origin
• Country of Origin: Report