Azure PVDF Membranes

Pre-cut PVDF membranes for Western blotting applications

Ideal for chemiluminescent and colorimetric Western blotting. Pre-cut to save you time in the lab. These low-fluorescence PVDF membranes reduce background noise and nonspecific protein binding for improved sensitivity. Available in three different pre-cut sizes.

Quantity: 10 membranes per package

Features

Low-fluorescence PVDF membranes reduce background noise for improved sensitivity. PVDF (polyvinylidene difluoride) membranes are commonly used for immunoblotting techniques, like Western blots. During the blotting process, samples are transferred from the protein gel onto a PVDF membrane by electroblotting. From there they can be used for protein sequencing.

• High binding capacity is great for low-expressing proteins
• Typically used for fluorescence
• High protein retention for stripping and reprobing

Azure Blotting Paper for Western Blotting

Pre-cut blotting paper for Western blot transfer

Forget about measuring and cutting blotting paper to the size of your mini-gels. Use these pre-cut papers to assemble your transfer “sandwiches”. Pre-cut blotting papers save time and reduce waste for the easiest Western blotting workflow.

Qty: 60 sheets

Blotting papers are cut to completely cover the gels and membranes.

For 8x10cm gels: blotting papers available in 9x13cm

For 7x9cm gels: blotting papers available in 8x10cm

Features

Pre-cut blotting papers save time and reduce waste for the easiest Western blotting workflow. Forget about measuring and cutting blotting paper to the size of your mini-gels! Use these pre-cut papers to assemble your transfer “sandwiches”.

Blotting papers come pre-cut to completely cover the gels and membranes.   
 
 
 

Hoefer® Semi-Dry Transfer Units

Hoefer® Semi-Dry Transfer Units are efficiently designed to use minimal buffer to transfer proteins from polyacrylamide gels onto membranes (either nitrocellulose or PVDF) in less than an hour. They support as many as two layers of gels being transferred simultaneously. The TE70XP has a 14 x 16 cm maximum transfer area, which can handle up to four mini gels using the stacked format. The TE77X and TE77XP have a 21 x 26 cm transfer area for a capacity of up to 12 mini gels at one time. 

• Included safety circuit breaker—limits voltage and current from the user's power supply, preventing electrical damage to the transfer unit
• Requires minimal current—does not generate the excessive heat that can dry out the transfer stack and halt transfer or damage transfer units 
• Durable iridium oxide and stainless steel electrodes—allow for contamination free, consistent transfer
• Vented electrodes—prevent buildup of bubbles which may impair transfer
• Minimal buffer requirements—reagent cost and preparation time are reduced
• The TE70XP and TE77XP Semi-Dry Transfer Units have an intelligent built-in power supply that prevents the stack from overheating by monitoring the transfer status 

All units include

• Molded Base with Platinum-Coated Titanium Anode and High Voltage Leads
• Hinged Lid with Stainless Steel Cathode  

TE77X includes

• Blotting Paper (21 x 26 cm), 25/pk
• Porous Cellophane (20 x 35.5 cm), 50/pk
• Mylar® Masks (23 x 27.5 cm), 2/pk
• Hinged Lid with Stainless Steel Cathode, 1/ea

TE70XP includes

• Blotting Paper (14 x 16 cm), 25/pk
• Porous Cellophane (20 x 35.5 cm), 50/pk
• Mylar® Masks (16.5 x 18.5 cm), 2/pk 

TE77XP includes

• Blotting Paper (21 x 26 cm), 25/pk
• Porous Cellophane (20 x 35.5 cm), 50/pk
• Mylar® Masks (23 x 27.5 cm), 2/pk

Specifications
 

Item #	TE77X	TE70XP	TE77XP
Transfer Area	Up to 21 x 26 cm Up to 8.27 x 10.24 In.	Up to 14 x 16 cm Up to 5.51 x 3.60 In.	Up to 21 x 26 cm Up to 8.27 x 10.24 In.
Max. Power	30 V, 500 mA, 15 W
Max. Temp.	113ºF | 45°C
Indoor Use	39.2 to 104ºF | 4 to 40°C
Humidity	Up to 80%
Dims (D x W x H)	3.54 x 14.96 x 18.11 In. | 9 x 38 x 46 cm
Weight	16.31 lb | 7.40 kg

Azure Flash Western Chemiluminescence Kit

Kit includes all reagents necessary for performing ten chemiluminescent Western blots using the Flash Western protocol

Each kit includes:

• 10x PVDF Membranes
• 100mL Chemi Blot Blocking Buffer
• 500μL Flash HRP Conjugate
• 250mL Flash Wash Buffer
• 100mL Radiance ECL Substrate

Features

Time savings using Azure Flash Western Chemiluminescent Kit. Flash Western graph includes use of Azure Transfer Buffer in light blue.

The Azure Flash Western Kit uses a proprietary set of reagents to achieve complete membrane blocking, rapid hybridization of antibodies, and enhancement of chemi signal in less than an hour of blotting time. The included transfer buffer cuts wet transfer time down to 15 to 30 minutes for a total assay time of two hours from gel to imaging.

• Fast protocol with few hands-on steps
• Antibody stabilization for enhanced chemi signal
• Low background and increased signal-to-noise ratios
• Increased detection sensitivity and linear dynamic range

Accuris Fast Extraction PCR Kit

The Accuris Fast Extraction PCR Kit features an exclusive single-tube solution that extracts genomic DNA in just 8 minutes. This is swiftly followed by PCR amplification utilizing our High Fidelity Hot Start Red Dye Master Mix, designed for superior endpoint PCR performance. High Fidelity Hot Start Red Dye Master Mix is a 2X formula that incorporates a non-reactive red dye, enabling direct gel loading.

This kit offers versatility to suit different workflows: the PCR-prepared genomic DNA can be directly used in endpoint PCR or can be applied in real-time PCR using either SYBR Green or TaqMan Probe chemistries.

• Offers exceptional versatility and can be used to extract and amplify DNA from a wide range of sample types
• Compatible with animal tissue, plant, saliva, & bacterial samples
• Extraction & Amplification in less than 60 minutes
• Includes High Fidelity Hot Start Red Dye Master Mix to prevent the risk of nonspecific amplification & to visualize bands easily
• Process extracted sample via PCR with included mastermix
• Alternatively, amplify via qPCR (qPCR mastermix not included) 

Each 100 reaction kit includes:

1 tube of High Fidelity Hot Start Red Dye Master Mix (1.25 mL)

1 tube of Fast Extraction Lysis Solution (1.25 mL)

Applications: Extraction & Amplification of Tissue, Plant, Saliva, & Bacteria
 

Proudly made in the USA! Click here for more made in America products!
 

Accuris SmartBlue™ Transilluminators

Accuris SmartBlue™ Transilluminators have been developed for viewing DNA gels stained with new, safe fluorescent dyes.  These new dyes, such as SmartGlow™, SYBR Green, Gel Green, have an excitation wavelength of 465-470 nm (visible blue light), and are safer to use than Ethidium Bromide.

• Blue light, safe for nucleic acids
• Uniform illumination (UI Technology)
• Optimal wavelength (465nm) for common safe stains
• Adjustable position, amber filter covers
• Optional amber viewing glasses: E4000-VG1

Safer for samples, safer for you: Accuris SmartBlue™ Transilluminators are the perfect partner for the most common safe DNA stains. These instruments emit blue light at a wavelength of 465 nm, perfect for excitation of most green fluorophors, including our Accuris’ SmartGlow™, SYBR Green, SYBR Safe, SYBR Gold, GelStar, GelGreen, to name a few.  SmartBlue™ transilluminators can also work for excitation of EtBr (though fluorescence is not as bright as with UV), see our Technical Bulletin with sample images.   Exposing DNA to blue wavelength does not cause molecular damage, and results in a much higher downstream cloning efficiency as compared to even short UV light exposures.  In addition, the visible blue light is not damaging to the user’s skin and is less harmful to the eyes.*

Adjustable filter cover: Both the E4101 and SB4500 transilluminators come with emission filter covers. These amber covers, which rest above the viewing surface and gel, filter out the blue light, allowing the fluorescing DNA bands to be seen clearly.  The covers can be set to a flat position for general gel viewing or can be set at an angle to provide easy access to the gel for band excision.  The SB4500 has a two-position filter cover, and the E4101 features a hinged filter cover.

Optional amber viewing glasses: Our E4000-VG1 SmartBlue™ Plus Viewing Glasses offer efficient blocking of the blue light wavelength from the transilluminators, allowing clear visualization of the fluorescing DNA bands without the filter cover in place.  These convenient glasses are recommended for users that are frequently excising DNA bands from gels.  SmartBlue™ Plus glasses also block 100% of UV wavelength so they can also be used for viewing gels on a UV transilluminator.

UI technology, bright and clear images: The bright LED light plates and filter layer provides an extremely evenly lit viewing surface. Even in ambient light, bands of DNA can clearly be seen in stained gels. Images obtained using the SmartBlue™ Transilluminator are brighter and more balanced than those obtained using epi-illuminators which light from the sides.

Reliable, durable, and long-lasting: An array of super bright LEDs with a long, 30,000 hour service life provide the light source for the SmartBlue™ Transilluminators. Unlike those in a UV transilluminator, the filters in the SmartBlue™ systems will not solarize and degrade in performance over time. Gel bands can be excised directly on the scratch resistant, glass viewing surfaces. To save energy, the power switch includes an automatic 5 minute shutoff. SmartBlue™ Transilluminators are covered by a 2 year warranty.

* SmartBlue™ products use high intensity LED lights.  Although the visible blue wavelength is significantly safer than UV light, it is recommended to not stare directly at the viewing surface without the amber cover in place. 

Specifications 

Light Source	High Intensity Blue LEDs
Emission Filter Cover	Amber
Wavelength	Peak at 465 nm
E4000 Viewing Surface	17 x 12 cm
E4000 Ext. Dimensions	30.5 x 21.5 x 5 cm
E4101 Viewing Surface	10.5 x 10.5 cm
E4101 Ext. Dimensions	17 x 12 x 5 cm
Electrical	100 to  240V, 50-60Hz
E4000 Weight	1.2kg / 2.6lbs
E4101 Weight	0.34kg / 0.75lbs
Warranty	2 years

 
 Item # SB4500 is proudly made in the USA! Click here for more made in America products!
 

Radiance ECL Chemiluminescent Substrate

Chemiluminescent HRP substrate for chemiluminescent Western blots

Radiance ECL is an HRP substrate with long-lasting signal and sensitivity down to low picograms. Use up to 10 times less antibody than other substrates used in life science.

Size: 500 mL

Features

• Performance — compare performance to SuperSignal West Pico and Amersham ECL
• Sensitivity — high sensitivity in low picograms
• Duration — Signal duration 6-8 hours
• Chemiluminescent Western Blotting
• The most common method for Western blot detection is chemiluminescence. Detection by chemiluminescence is relatively sensitive and works well for detecting individual or low abundance proteins.   

Radiance Plus Chemiluminescent Substrate

The most sensitive HRP substrate available for chemiluminescent Western blotting.

With attomole sensitivity and a long-lasting signal, Radiance Plus allows you to detect bands not visualized with other substrates. High signal-to-noise and a large dynamic range make it ideal for quantifying low-intensity bands.

Size: 150 mL

Features

• Low-abundance protein detection
• Compare performance to competitor products, like SuperSignal West Femto and Amersham ECL Select
• Sensitivity in low femtograms
• Signal duration 6-8 hours   

Radiance Q Chemiluminescent Substrate

Radiance Q chemiluminescent substrate is specially formulated for use with Azure Imaging Systems, or other CCD imagers. This novel horseradish peroxidase (HRP) substrate provides a strong, long-lasting signal, the broadest useful linear range, and high sensitivity for the most quantitative chemiluminescent Western assays.

Size: 150 mL

Features
 

Increase the sensitivity and quantitative accuracy of your chemiluminescent Western blots. Specially developed for CCD imaging, Radiance Q produces a strong, long-lasting signal with extremely low background, perfect for detecting low abundance proteins. Because the substrate is not depleted at high protein loads, Radiance provides the largest dynamic range of any chemiluminescent substrate, improving quantitative accuracy.

• Extended dynamic range for detecting low and high abundance proteins within the same blot
• Compare performance to SuperSignal West Dura and Amersham ECL Prime
• Sensitivity in mid femtograms
• Signal duration 10-24 hours   

PurePlus® Deep Well Plates

Labcon’s PurePlus® Deep Well Plates are rigorously tested to ensure they are free of RNase, DNase, ATP, and endotoxins. With our in-house microbiology lab, consistency is a top priority.

Available in sterile and non-sterile formats, these plates fit most popular instruments and can be sealed with sealing films or reusable silicone mats. Barcoding is available for both 1.2 mL and 2.2 mL plates by special order.

Ultra Clean & Ultra Clear 

PurePlus® plates feature molded-in orientation and permanent printed locating grids for ease of use. They are available in multiple formats: 96 well square hole with conical or flat bottom, 96 well round hole with round bottom, and 384 well square hole.

With volumes ranging from 0.2 mL to 2.5 mL, our plates are offered in non-sterile and validated medical-grade sterile versions, sterilized to an SAL of 10^-6 in compliance with ISO 11137.

The 1.2 mL and 2.2 mL volumes include three-sided alpha-numeric labeling grids, and their smooth interior surfaces ensure efficient pipetting. Designed for manual or robotic handling, these plates are SBS formatted for compatibility with automated systems.

Additional Features

Made from 100% virgin polypropylene, PurePlus® plates are autoclavable and freezable. Unlike "pre-sterile" products from other manufacturers, our sterile plates are fully validated for diagnostic and regulated procedures.

Specifications

ILP Square Well, V Bottom Plates, 120 uL - 384

Our 384-well plates offer quality, performance, and confidence. Ultra-flat surfaces, sturdy design, and rigidity optimize our 384-well plates for consistent handling with a variety of automated platforms for high-throughput workflows. Plates are standardized to comply with ANSI/SBS 6-2012, guaranteeing proper fit.

These plates offer secure storage for assays, compound libraries, or storing samples for any duration. Inert medical-grade virgin polypropylene resins are ideally suited for stable, long-term high-density sample storage at -80 °C. The square well V bottom design helps maximize sample recovery. Raised rims on each well help protect from cross-contamination and make for enhanced sealing with adhesive films, silicone mats, or heat-sealing films.

Made from Virgin Polypropylene (no mold release agents or regrind); RNase, DNase, DNA, and Pyrogen Free Certification.

Specifications
 

Product Type	384 Square Well Plates
Well Shape Bottom	Pyramid – V
Material	Natural Polypropylene
Alphanumeric	Molded in
Compliance	ANSI/SBS 6-2012
Surface Treatment	None
Certified	DNase-/RNase-/Pyrogen-Free
Max Volume	130 uL
Working Volume	120 uL
Volume w/ Mat Lid	30 uL
Autoclavable	121ºC (249.8ºF) at 15 psi, 15 min.
Max RCF	4000
Max Temp.	120ºC (248ºF)
Min Temp.	-80ºC (-112ºF)