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PCR/qPCR
MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more. If you are looking for any of these things, we can match almost all needs to all cyclers. If you don’t find it easily contact us at tech@midsci.com and we will find it for you.
96 or 384 we have it. Non-Skirted, semi-skirted or full skirted, we have it. 0.1, 0.2, or 0.5 mL we have it. A1, A12, H12, A24, P24 cuts, we have them. Sybr© Green or probe alternative, we have it.
Pro Tip: Colored plastics do not have any impact on DNA amplification. But when setting up qPCR it is often recommended to use white plastics. This will limit or prevent fluorescence refraction. Light refraction can minimize sensitivity and consistency.
Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips. This will help optimize and reduce variance in the reactions. Simple test, see the residual liquid left in tips? This means some of the taq, dNTPs, sample, etc is left in the tip. PR1MA tips help reduce that significantly and improve your consistency.
Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate? Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler. Call us we have solutions.
This Item has been discontinued.
See Azure Biosystems Cielo qPCR System instead!
MyGo Mini S
- Small format 16 x 0.1 mL QPCR System with Taqman Multiplexing at an amazing price.
- Amazing accuracy and easy to use software that allows you to control 100s of systems with one PC and analyze the data at the same time. Windows, Linux and Mac OSX
- Solid state reliability with up to 5-year warranty
Precision and Performance
The MyGo Mini demonstrates superior intra-run and inter-run analytical precision in DNA quantification and melting point analysis. Users can now address biological phenomena with subtle effects on gene expression, or pathogen levels, for example discriminating 10% differences in transcript concentrations. For users performing HRM-based analysis of genetic variants, the combination of thermal control, optical data quality and HRM data analysis of the MyGo Mini S system provide compelling functionality. The system can discriminate all classes of SNP, including Class 4 SNPs via HRM.
Users can now address biological phenomena with subtle effects on gene expression, or pathogen levels, for example discriminating 10% differences in transcript concentrations.
For users performing HRM-based. analysis of genetic variants, the combination of thermal control, optical data quality and HRM data analysis of the MyGo Mini system provide compelling functionality. The system can discriminate all classes of SNP, including Class 4 SNPs via HRM.
Easy Analysis
The MyGo Mini system comes with easy-to-use software, and robust, high performance algorithms which provide accurate results from complex data. Automated analysis of complex HRM data makes this powerful technique more accessible to non-experts, and minimizes operator-dependent variability in data analysis. Automated report generation makes life easier for service providers, and users operating within GLP quality systems.
Automated report generation makes life easier for service providers, and users operating within GLP quality systems.
This Item has been discontinued.
See the Azure Biosystems Cielo qPCR System instead!
MyGo Pro ESR
The MyGo Pro real-time PCR instrument provides unmatched performance in a convenient format. Novel Full Spectrum Optics deliver 120 optical channels of fluorescence data from every tube in parallel, with no moving parts, for reliable multiplex PCR.
Precision And Performance
The MyGo Pro demonstrates superior intra-run and inter-run analytical precision in DNA quantification and melting point analysis.
Users can now address biological phenomena with subtle effects on gene expression, or pathogen levels, for example discriminating 10% differences in transcript concentrations.
For users performing HRM-based analysis of genetic variants, the combination of thermal control, optical data quality, and HRM data analysis of the MyGo Pro system provides compelling functionality. The system can discriminate all classes of SNP, including Class 4 SNPs via HRM.
Multiplex With Full Spectrum Optics
Novel Full Spectrum Optics provide 120 channels of wavelength-dependent optical data from every well every time. No other qPCR system offers this number of optical channels.
Full Spectrum Optics, combined with advanced matrix deconvolution technology, enable the simultaneous analysis of at least 7 different targets in a single reaction, each monitored with a different color of fluorescent label.
The MyGo Pro comes pre-calibrated for 20 different fluorescent labels, and the software enables users to use any dye with an emission maximum between 510nm and 750nm. No other qPCR system provides the user with this level of flexibility.
Easy Analysis
The MyGo Pro system comes with easy-to-use software, and robust, high-performance algorithms provide accurate results from complex data.
Automated analysis of complex HRM data makes this powerful technique more accessible to non-experts and minimizes operator-dependent variability in data analysis.
Automated report generation makes life easier for service providers, and users operating within GLP quality systems.
Reliability
The MyGo Pro has been designed to last, with very few moving parts, made and tested to exacting standards. The result is a highly reliable instrument, which comes with the option of a 5-year extended warranty.
Speed
With heating rates of 5°C per second and cooling rates of 4°C per second the MyGo Pro is one of the fastest systems available. No other system matches this speed with convenient-to-use disposables. The system demonstrates excellent quantitative precision and speed, with 45 cycles of PCR in 33 minutes. Typical qPCR systems take much longer than this to deliver results.
Compatibility
The MyGo Pro software can be installed on Mac OS X, Windows, and Linux operating systems. Use your computer and your choice of operating system.
Connectivity
The MyGo Pro software does not require a dedicated computer, so users do not need to find the space or money, for an additional computer to run the system. In addition, the software enables the user to control multiple instruments, and simultaneously analyze multiple runs, with one computer.
This reversible rack comes with a simple-to-use removable hinged lid that snaps in place on either side of the rack. Rack and lid are molded out of autoclavable polypropylene. PCR® side of the rack has 168 wells that hold 0.2mL tubes. The wells will accommodate 8 or 12 tube strips or individual tubes. The opposite side of the rack has 40 wells that hold 0.5mL tubes. We have also placed 12 wells for 1.5mL tubes on both sides of the rack for your convenience. Available in natural or assorted colors (blue, green, purple, yellow, and orange). Packs of 5. PCR® is a registered trademark of Hoffman-LaRoche Inc.
Our rack is perfect for pre- or post-PCR® sampling applications. Sturdy polypropylene rack accommodates individual 0.2mL tubes or strips of 8 or 12 tubes. Tube wells are easily identified with imprinted numbers and letters. Features a removable hinged lid. Available in natural, blue, green, pink, yellow, or assorted colors (one of each). Packs of 5. PCR® is a registered trademark of Hoffman-LaRoche Inc.
PR1MA™ LD032 Mini Thermal Cycler
- "Mini" sized, 9 x 7 inch footprint
- Capacity for 32 x 0.2 mL or 4 PCR Strips
- Intuitive touchscreen control
"Mini-sized" doesn't have to mean a sacrifice on features and performance. PR1MA™'s LD032 Mini Thermal Cycler offers advanced programming features, easy-to-use software, and fast, reliable cycling but with a smaller block and lower price point.
Designed for lower throughput needs, the PR1MA™ LD032 Mini Thermal Cycler has a capacity for 32 x 0.2 mL tubes. The wells are arranged in a 4 x 8 format, allowing the block to accept strips of 8 as well as individual tubes. The block heats and cools very quickly - at rates as fast as 5°C/second. A proprietary algorithm controls block temperature, bringing samples quickly to the target temperature without any over- or under-shoot. Excellent block uniformity ensures consistency from sample to sample.
The operation of the thermal cycler is controlled from the large, full-color, touch screen. Icon-driven menus allow users to get running quickly. Programming is extremely simple and includes options for time and temperature increments/decrements, adjustable ramp rates, and auto pause and restart functions. More than 100 typical programs can be stored in memory and easily recalled. After a run, samples can be held at 4°C. An on-screen report is also available at the completion of a run.
The heated lid is self-adjusting - the spring-loaded platen in the lid comes firmly into contact with the tops of the tubes to prevent sample evaporation and condensation. There are no knobs to turn or levers to adjust. The temperature of the heated lid can be changed, or the heating function of the lid may be turned off.
With a footprint less than that of the average laboratory notebook, the PR1MA™ LD032 Mini Thermal Cycler will fit almost anywhere. Its reduced capacity block and economical price make the PR1MA™ LD032 Mini Thermal Cycler the perfect choice for lower throughput laboratories and educational facilities.
Specifications
| Capacity - 0.2 mL tubes | 32 |
| Capacity - 8 x 0.2 mL PCR strips | 4 |
| Temp. Control Range | 0° to 99°C |
| Temp. Control | Simulated sample or block |
| Accuracy/Uniformity | ± 0.25°C | ± 0.25°C |
| Ramp Rate | Heating: 5°C / Sec. (max) Cooling: 5°C / Sec. (max) |
| Lid Temp | 30°C to 112°C, user adjustable |
| Program Memory | Over 100 typical programs |
| Increments/Decrements | Yes, both time and temperature |
| Power Failure Restart | Yes |
| Power Supply | Universal,100 to 240 V, 50 to 60 Hz |
| Dimensions | 7.2 x 9.2 x 6.2 in. | 18.2 x 23.2 x 15.7 cm |
| Weight | 6.4 lbs. | 2.9 kg |
| Warranty | 2 years |
PR1MA™ Mini Gel Electrophoresis System
- Complete system, includes gel box, power supply and casting sets
- Quick and easy gel casting, no tape, clamps or spacers required
- Compact design, footprint: 7 x 5.1 in.
The PR1MA™ Mini Gel electrophoresis system includes all of the equipment that you need to get up and running: gel tank, power supply, and two casting sets. Ideal for personal use, small laboratories, or the classroom, this gel box/power supply combination is compact and easy to use.
The mini gel box attaches directly to the power supply for a secure connection every time. Selectable parameters include 3 preset voltages – 35V, 50V or 100V – and a 99-minute timer. All are set using the membrane keypad.
The safety lid is vented to help dissipate heat generated during electrophoresis and features a viewing area above the gel. Current will only run through the system when the lid is properly in place on top of the gel box.
Trays and casting stands for both 10.5 x 6 cm and 5 x 6 cm gels are included with the PR1MA™ Mini system, as are double-sided combs. The smaller tooth combs are compatible with multi-channel pipettes. Casting is quick and easy – just place the tray in the casting stand and pour. The trays feature a dark contrast strip to help visualize wells while loading and lines that fluoresce under UV and blue light to track the progress of the run.
Specifications
| Output Voltage | 35V, 50V or 100V |
| Timer | 0-99 min. or Continuous |
| Safety | Lid interlock |
| Max. Buffer | 230 mL |
| Gel Capacity | One: 10.5 x 6 cm |
| Two: 5 x 6 cm | |
| Weight | 1 lbs | 0.45 kg |
| Dimensions | 7.5 x 5.1 x 2.1 in |
| Electrical | 115V or 230V, 50-60 Hz |
- Convenient cDNA synthesis and PCR in a single tube from 1 pg total RNA
- Formulated for highly specific and sensitive RT-PCR from any RNA templates
- Incorporates thermostable reverse transcriptase and Hot-Start Taq for preparation at room temperature
The PR1MA One-Step RT-PCR Kit has been formulated for cDNA synthesis and subsequent PCR in a single tube for end-point analysis. This latest generation RT-PCR Kit consists of an MMLV-derived, thermostable Reverse Transcriptase (45°C to 55°C), an advanced RNase Inhibitor and PR1MA Hot Start Taq for ultra-sensitive one-step RT-PCR from as little as 1pg total RNA starting material.
The optimized buffer chemistry allows for efficient reverse transcription and PCR of problematic sequences with significant secondary structure (such as GC-rich targets). The PR1MA One-Step RT-PCR Kit is ideal for determining the presence or absence of RNA templates and quantifying expression through qualitative analysis of RNA transcription levels. The kit also efficiently synthesizes double-stranded cDNA for subsequent gene expression analysis.
PCR Cooler, 96 Well
Ice-Free Cooling System for PCR Preparation and Storage
- Modern design cools faster in freezers and stays cold longer on the bench top
- Maintains the entire 96-well plate at 0C for over an hour
- Maintains sample temperature of less than 5C for up to three hours (with lid off)
- When the temperature ceiling of 6C has been exceeded there is a contrasting color change of the cooler or a single well
- Cools industry standard format PCR tubes, strips and single format as all as most 96-well plates in both standard and low profile well shape
- Patented lid design provides seamless storage and transport by securely covering the PCR samples from the freezer to the bench top
- Innovative lid slides out, rotates 180 degrees, and becomes a convenient stand the cooler sits on
- Well design prohibits the freezing of samples
- Compact space saving design, stackable
- Cooler made from engineered polypropylene and lid is made from polycarbonate
- Two-year warranty
PR1MA™ qMAX™ cDNA Synthesis Kits
PR1MA™ now offers two cDNA Synthesis Kits, to meet a range of requirements.
The original cDNA Synthesis Kit is a 2-tube format for easy reaction setup and is ideal for 4 pg to 0.5 µg of input RNA. One tube includes our exceptionally stable Reverse Transcriptase combined with a potent RNAse inhibitor, and the other tube contains a 5X reaction buffer with an optimal mixture of anchored oligo (dT) primers and random hexamers to produce a non-biased population of cDNA.
The 1st Strand cDNA Synthesis Flex Kit includes four components: a high-capacity Reverse Transcriptase, an optimized 5X buffer, separate solutions of oligo (dT) primers, and random hexamer primers. This multi-component format is ideal for 10pg to 2.0µg of input RNA and allows for greater flexibility in assay design.
PR1MA™ qMax cDNA Synthesis Kits
| Item | Description | Volume (20 µL) |
| PR2100-C-S | PR1MA™ qMax™ cDNA Synthesis Kit | 10 Reactions (Sample) |
| PR2100-C-25 | PR1MA™ qMax™ cDNA Synthesis Kit | 25 Reactions |
| PR2100-C-100 | PR1MA™ qMax™ cDNA Synthesis Kit | 100 Reactions |
| PR2100-C-250 | PR1MA™ qMax™ cDNA Synthesis Kit | 250 Reactions |
PR1MA™ qMax First Strand cDNA Synthesis Flex Kits
| Item | Description | Volume (20 µL) |
| PR2110-S | PR1MA™ qMax™ First Strand cDNA Synthesis Flex Kit | 10 Reactions (Sample) |
| PR2110-50 | PR1MA™ qMax™ First Strand cDNA Synthesis Flex Kit | 50 Reactions |
| PR2110-200 | PR1MA™ qMax™ First Strand cDNA Synthesis Flex Kit | 200 Reactions |
however, we will be continuing to supply the other quantities.
In place of item PR2110-100, please order items PR2110-50 or PR2110-200
PR1MA™ dNTPs
- Supplied as a ready-to-use 40mM mix or a set of 4 separate 100mM solutions
- Free of impurities and inhibitors that reduce sensitivity and yield
- No nuclease, protease or nickase activity
- >99% pure, purified by HPLC
- 24-month shelf life
PR1MA™ dNTP's are purified by HPLC in a strict process that results in greater than 99% purity. The stringent purification process eliminates PCR inhibitors such as tetraphosphates and pyrophosphates that can interfere with the sensitivity of your PCR and reduce yields.
The 40mM dNTP Mix is a single tube that contains premixed dNTPs at a concentration of 10mM each. The 100mM dNTP Set contains four individual tubes, one each of dATP, dCTP, dGTP and dTTP. The nucleotides are supplied in ultra-pure water as an ammonium salt. Both the set and mix are stable for 24 months when stored at -20ºC.
Extensive quality control testing is performed to ensure the dNTPs are free of nuclease, protease and nickase activity. Each lot is performance tested in standard PCR, long PCR and qPCR reactions to assess reproducibility and sensitivity.
PR1MA™ dNTP Mix
| Item | Description | Volume |
| PR3040-M-1 | PR1MA™ 40 mM dNTP Mix, Ready-to-use | 0.5 mL |
| PR3040-M-2 | PR1MA™ 40 mM dNTP Mix, Ready-to-use | 1 mL |
| PR3040-M-4 | PR1MA™ 40 mM dNTP Mix, Ready-to-use | 2 mL |
| PR3040-M-8 | PR1MA™ 40 mM dNTP Mix, Ready-to-use | 4 mL |
PR1MA™ dNTP Set
| Item | Description | Volume |
| PR3101-S-1 | PR1MA™ 100 mM dNTP Set, dATP, dCTP, dGTP, dTTP | 250 µL/25 µmol each |
| PR3101-S-4 | PR1MA™ 100 mM dNTP Set, dATP, dCTP, dGTP, dTTP | 1 mL/100 µmol each |
| PR3101-S-20 | PR1MA™ 100 mM dNTP Set, dATP, dCTP, dGTP, dTTP | 20 x 250 µL/25 µmol each |
Mystaire® CleanPrep™ dead air boxes are circulation-free enclosures that establish an ideal work area to protect against contamination in sensitive PCR amplification reactions. CleanPrep™ workstations are constructed from thermally-fused polypropylene that offers exceptional chemical resistance, does not adsorb moisture and can be easily cleaned and decontaminated between amplifications using a variety of disinfectant solutions.Seamless polypropylene design for easy decontamination between amplifications.
FEATURES:
- Clear polycarbonate access with overlap and safety position switch
- UV shelf with integrated pipette holder - accommodates four pipettors
- High intensity fluorescent lamps and shortwave UV light
- Available in 36 and 48 inch widths with nominal 24 inch depth
- Circulation-free enclosure for PCR applications
Technical Specifications |
|||||
Specifications |
MY-DB24 |
MY-DB36 |
MY-DB48 |
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Physical Dimensions (W x H x D inches) |
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Outside Dimensions |
24 x 30 x 24 |
36 x 30 x 24 |
48 x 30 x 24 |
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Inside Dimensions (usable work area) |
23 x 20 x 23 |
35 x 20 x 23 |
47 x 20 x 23 |
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Weight |
45 lb. |
60 lb. |
80 lb. |
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Airflow Characteristics |
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Low Airflow Alarm |
N/A |
N/A |
N/A |
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Volume of Filtered Air |
N/A |
N/A |
N/A |
||
Linear Face Velocity, Sash Closed |
N/A |
N/A |
N/A |
||
Linear Face Velocity |
N/A |
N/A |
N/A |
||
Electrical |
|||||
Voltage |
110 V or 220 V |
110 V or 220 V |
110 V or 220 V |
||
Current |
1.4 A/0.7 A |
1.4 A/0.7 A |
2.8 A/1.4 A |
||
Power Consumption |
115 W |
115 W |
115 W |
||
Operation |
|||||
Speed Selection |
N/A |
N/A |
N/A |
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Construction |
|||||
Chamber |
Structural Polypropylene |
Structural Polypropylene |
Structural Polypropylene |
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Front Panel |
Polycarbonate |
Polycarbonate |
Polycarbonate |
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Prefilter (On top housing) |
|||||
Type (particulate) |
N/A |
N/A |
N/A |
||
Weight |
N/A |
N/A |
N/A |
||
Dimensions |
N/A |
N/A |
N/A |
||
Requirements |
N/A |
N/A |
N/A |
||
Filter |
|||||
Type (Provides HEPA Clean Work Area) |
N/A |
N/A |
N/A |
||
Weight |
N/A |
N/A |
N/A |
||
Dimensions |
N/A |
N/A |
N/A |
||
Requirements |
N/A |
N/A |
N/A |
||
HEPA Filters, Pre-Filters, UV bulbs, additional ports and shelves also available.
PR1MA™ Hot Start Taq
- Exceptional sensitivity for low-copy PCR
- Ideal for multiplex PCR and amplification of GC-rich DNA
- Same enhanced features as PR1MA Taq Polymerase
- Buffer optimized for fast cycling and reproducibility
Engineered for controlled polymerase activity, PR1MA Hot Start Taq is bound with a monoclonal antibody that blocks enzyme activity. This allows reactions to be set up at room temperature without the risk of non-specific amplification.
When samples are ready, the reaction mixture is heated to 95°C to denature the antibody and initiate the reaction. Similar to the standard PR1MA Taq, Hot Start Taq is provided with a 5X buffer, or in a ready-to-use 2X Master Mix. The Master Mix can be ordered with or without an incorporated red gel loading dye. The Red Dye Master Mix incorporates a red-loading dye that allows amplified samples to be loaded directly on an agarose gel. The red color aids in visualization during pipetting, and higher density of the buffer ensures that the samples will drop into the gel wells.
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