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PR1MA™ MS2 Phage
PR1MA™ MS2 is an E. coli bacteriophage with a single-stranded RNA genome of 3569 nucleotides protected from nuclease degradation by a capsid of 180 coat protein monomers. This virus is a Biosafety Level 1 organism, not pathogenic to humans. These properties make MS2 phage useful as a process control in nucleic acid-based amplification techniques like RT-PCR, RT-LAMP, particularly those that involve viral RNA extraction.
- Biosafety level 1
- Lysis: 65°C for 20 minutes or by standard RNA extraction
- Storage temperature: 4°C
Buffer composition
- 10 mM Tris-HCl
- 0.1 mM EDTA
- pH = 8.0
*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.
Proudly made in the USA! Click here for more made in America products!
PR1MA™ RNase Inhibitor
PR1MA™ RNase Inhibitor (RI) is a 50 kDa protein that specifically inhibits RNases A, B, and C by binding noncovalently in a 1:1 ratio at high affinity. It can be used in isothermal amplification and molecular diagnostic assays, cDNA synthesis, and other applications where RNA stability is important. It is ineffective against RNase1, T1, S1 Nuclease, or RNase H. It has no inhibition of polymerase activity when used with Taq DNA polymerase, AMV, M-MuLV, HIV reverse transcriptases, or phage RNA polymerases.
Storage Buffer
- 50% glycerol
- 10 mM Tris-HCl
- 50 mM KCl
- 8 mM DTT
- pH = 7.5
*To prevent the release of ribonuclease from RNase Inhibitor, temperatures greater than 50°C and high concentrations of denaturing reagents such as urea should be avoided.
*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.
Proudly made in the USA! Click here for more made in America products!
PR1MA™ β - Agarase
PR1MA™ β - Agarase has a high tolerance to inhibitors in electrophoretic buffers such as TBE and TAE, eliminating the need to perform a buffer exchange step before digestion. PR1MA™ β - Agarase has higher thermal stability than other commercially available β - agarases, with a broad range of activity between 42°C and 50°C. Higher temperature digestion reduces the chances of residual agarose gelling at the end of the reaction, resulting in higher recovery of DNA or RNA.
Benefits
- Complete digestion of agarose, with no agarose fragments, left after the digestion
- Obtain DNA or RNA faster - no buffer exchange needed, with direct digestion in TAE or TBE
- Concentration: 1000 u / mL
- Operating temperature: 42°C to 50°C
- Recommended temperature: 50°C
- Storage temperature: -20°C
Storage Buffer
- 50% glycerol
- 50 mM Tris-HCI
- 50 mM KCI
- 1 mM DTT
- 0.1 mM EDTA
- pH = 7.5
*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.
Proudly made in the USA! Click here for more made in America products!
PrecisionPak Plant Matter DNA Kit
All-inclusive kit, from lysis to extraction, including protocol, lysis beads, all buffers and reagents, to ensure high quality DNA.
The PrecisionPak Plant Matter DNA extraction kit contains a combination of lysis beads preloaded in sample tubes and lysis buffer for thorough homogenization and high yield. The buffers' formulations are environmentally friendly and do not require ethanol, phenol, chloroform, and isopropanol. The extraction kit is innovatively designed to be faster and more efficient than spin-column methods.
Tough Plant Matter DNA Kit available for: Barley, Beans, Blueberry, Cannabis, Chickpeas, Cloves, Conifer Needles, Corn, Dried Soy Beans, Fungi (M.phaseolina), Ginger Rhizome, Horseradish Root, Leaves, Leek, Malanga (Tuber), Microsporidia, Moss, Mung Beans, Mycelia, N. benthamiana, Pine Nut, Potato, Rice, Soy Bean Plant, Spelt, Sugar Cane Pulp, Sunflower Seeds, Sorghum, Tobacco, Tomato Root, Tomato Seeds, Wheat Berries.
Soft Plant Matter DNA Kit available for: Algae, A thaliana, and Phytoplankton.
PrecisionPak Animal Tissue RNA Kit
All-inclusive kit, from lysis to extraction, including protocol, lysis beads, all buffers and reagents, magnetic extraction beads, and additional reagents to ensure high-quality RNA.
The PrecisionPak Animal Tissue RNA extraction kit contains a combination of lysis beads preloaded in sample tubes and lysis buffer for thorough homogenization and high yield. Magnetic bead technology and additional reagents such as Proteinase K and DNase ensure very high-quality RNA. The magnetic bead technology is innovatively designed to be faster and more efficient than spin-column methods.
Tough Animal Tissue RNA Kits available for: Aorta, Bladder, Cartilage, Cecum, Colon, Diaphragm, Duodenum, Ear, Eye, Feces, Heart, Intestinal Mucosa, Intestine, Jejunum, Larynx, Meconium, Mouse Femur and Tibia, Muscle, Nasal, Olfactory Mucosa, Oropharynx, Placenta, Polyp, Skin, Spinal Cord, Stomach, Tail Snips, Testes, Thalamus, Toe Clip, Tongue, Trachea, Tumor, Umbilical Cord, and Uterus.
Soft Animal Tissue RNA Kit available for: Adipose, Adrenal Gland, Blood, Blood Vessels, Brain, Cerebral Fluid, Gall Bladder, Hypothalamus, Kidney, Liver, Lung, Lymph Node, Marrow, Nerve, Pancreas, Pharynx, Pituitary Gland, Salivary Gland, Spleen, Thymus, and Thyroid.
PrecisionPak Animal Tissue DNA Kit
All-inclusive kit, from lysis to extraction, including protocol, lysis beads, all buffers and reagents, magnetic extraction beads, and additional reagents to ensure high-quality DNA.
The PrecisionPak Animal Tissue DNA extraction kit contains a combination of lysis beads preloaded in sample tubes and lysis buffer for thorough homogenization and high yield. Magnetic bead technology, coupled with additional reagents such as Proteinase K and RNase ensures very high-quality DNA. The magnetic bead technology is innovatively designed to be faster and more efficient than spin-column methods.
Tough Animal Tissue DNA Kit available for: Aorta, Bladder, Cartilage, Cecum, Colon, Diaphragm, Duodenum, Ear, Eye, Feces, Heart, Intestinal Mucosa, Intestine, Jejunum, Larsynx, Meconium, Mouse Femur and Tibia, Muscle, Nasal, Olfactory Mucosa, Oropharynx, Placenta, Polyp, Skin, Spinal Cord, Stomach, Tail Snips, Testes, Thalamus, Toe Clip, Tongue, Trachea, Tumor, Umbilical Cord, and Uterus.
Soft Animal Tissue DNA Kit available for: Adipose, Adrenal Gland, Blood, Blood Vessels, Brain, Cerebral Fluid, Gall Bladder, Hypothalamus, Kidney, Liver, Lung, Lymph Node, Marrow, Nerve, Pancreas, Pharynx, Pituitary Gland, Salivary Gland, Spleen, Thymus, and Thyroid.
PrecisionPak Small Organism DNA Kit
All-inclusive kit, from lysis to extraction, including protocol, lysis beads, all buffers and reagents, magnetic extraction beads, and additional reagents to ensure high quality DNA.
The PrecisionPak Small Organism DNA extraction kit contains a combination of lysis beads preloaded in sample tubes and lysis buffer for thorough homogenization and high yield. Magnetic bead technology, coupled with additional reagents such as Proteinase K and RNase ensure very high-quality DNA. The magnetic bead technology is innovatively designed to be faster and more efficient than spin-column methods.
Tough Small Organism DNA Kit available for: Caterpillar, Crayfish, Crickets, Fruit Fly (Drosophila), Honeybee, Mosquito, Shrimp, Ticks, Zebrafish (D. rerio), Xenopus Tissue.
Soft Small Organism DNA Kits available for: Hookworm, Nematode (B. xylophilus), Roundworm (C. elegans), and Zooplankton.
PrecisionPak Small Organism RNA Kit
All-inclusive kit, from lysis to extraction, including protocol, lysis beads, all buffers and reagents, magnetic extraction beads, and additional reagents to ensure high-quality RNA.
The PrecisionPak Small Organism RNA extraction kit contains a combination of lysis beads preloaded in sample tubes and lysis buffer for thorough homogenization and high yield. Magnetic bead technology, coupled with additional reagents such as Proteinase K and DNase ensures very high-quality RNA. The magnetic bead technology is innovatively designed to be faster and more efficient than spin-column methods.
Tough Small Organism RNA Kits available for: Caterpillar, Crayfish, Crickets, Fruit Fly (Drosophila), Honeybee, Mosquito, Shrimp, Ticks, Zebrafish (D. rerio), Xenopus Tissue.
Soft Small Organism RNA Kit available for: Hookworm, Nematode (B. xylophilus), Roundworm (C. elegans), and Zooplankton.
MagBio HighPrep™ Viral/Pathogen DNA/RNA Kit
Magnetic beads based kit for rapid isolation of viral, bacterial and fungal nucleic acids from whole blood, serum, plasma, saliva and other body fluids.
- RT-qPCR; RT-PCR, PCR
- One-Step RT-qPCR
- Virus detection, genotyping
- Viral load monitoring,
- OPTIMIZED FOR ISOLATION from FUNGAL, BACTERIAL and VIRAL samples.
- Rapid and reliable purification of nucleic acids
- Adaptable to various automated liquid handling workstations
- No toxic organic solvents
The HighPrep™ Viral/Pathogen DNA/RNA kit is designed for rapid and reliable isolation of viral, bacterial and fungal nucleic acids from whole blood, serum, plasma, saliva and other body fluids as well as nasopharyngeal swabs soaked in virus transport media or other buffers. This kit is highly efficient in vIral nucleic acid isolation and the extracted RNA (and DNA) is suitable for direct use in most downstream applications such as one-step RT-qPCR, RT-PCR, PCR, nucleic acid amplification, cloning, sequencing, and enzymatic reactions. The kit can be used in low throughput manual workflows and is also adaptable to majority of the liquid handling workstations in the market.
, Beads
The I-Blue MINI Plasmid Kit was designed for rapid isolation of plasmid DNA from 1-7 mL of cultured bacterial cells. I-Blue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. Typical yields are 20-35 µg for high-copy number plasmid or 3-10 µg for low-copy number plasmid from 4 mL of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 15 minutes. The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions.
Advantages
- Sample: 1-7 mL of cultured bacterial cells
- Yield: Up to 50 µg of pure plasmid DNA
- Format: Plasmid spin column
- Operation Time: Within 15 minutes
- Elution Volume: 30-100 µL
- Kit Storage: Dry at room temperature (15-25°C) for up to 1 year; PD1 and RNase A mixture should be stored at 2-8°C for up to 6 months
Quality Control
The quality of the I-Blue MINI Plasmid Kit is tested on a lot-to-lot basis by isolating plasmid DNA from a 4 mL overnight E. coli (DH5α) culture containing plasmid pBluescript (A600 > 2 U/mL). Following the purification process, a yield of more than 20 µg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid DNA (1 µg) is used in EcoRI digestion, and analyzed by electrophoresis.
The I-Blue MINI Plasmid Kit was designed for rapid isolation of plasmid DNA from 1-7 mL of cultured bacterial cells. I-Blue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. Typical yields are 20-35 µg for high-copy number plasmid or 3-10 µg for low-copy number plasmid from 4 mL of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 15 minutes. The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions.
Advantages
- Sample: 1-7 mL of cultured bacterial cells
- Yield: Up to 50 µg of pure plasmid DNA
- Format: Plasmid spin column
- Operation Time: Within 15 minutes
- Elution Volume: 30-100 µL
- Kit Storage: Dry at room temperature (15-25°C) for up to 1 year; PD1 and RNase A mixture should be stored at 2-8°C for up to 6 months
Quality Control
The quality of the I-Blue MINI Plasmid Kit is tested on a lot-to-lot basis by isolating plasmid DNA from a 4 mL overnight E. coli (DH5α) culture containing plasmid pBluescript (A600 > 2 U/mL). Following the purification process, a yield of more than 20 µg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid DNA (1 µg) is used in EcoRI digestion, and analyzed by electrophoresis.
PCR DNA Clean-up Kit
The 96-well PCR Clean-Up/DNA Extraction Kits provide a high-throughput, rapid and economical method to purify DNA fragments. Chaotropic salt is used to denature enzymes and in this condition, DNA fragments are bound by the glass fiber matrix in each well of the plate. Once the contaminants have been removed, the purified DNA is eluted by a low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides are effectively removed from reaction mixtures without toxic phenol extraction or alcohol precipitation. This entire protocol can be completed in 30-40 minutes, and the eluted DNA is ready to use in restricted digestion, ligation, PCR, and sequencing reactions.
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