Mystaire® PCR Prep Stations

They are compact laminar flow enclosures that reduce the risk of sample contamination while performing polymerase chain reaction experiments.

Airborne contaminants are prevented from entering into the work zone through constant positive pressure HEPA filtered air. Each PCR prep station is equipped with a built-in 254 nanometer shortwave lamp to effectively decontaminate the work area between amplifications. Safety interlocks are included on all PCR prep stations to prevent operator exposure to potentially harmful UV radiation.

MY-PCR prep stations are bench-top personal clean zones allowing the operator to amplify RNA and DNA while minimizing the potential for background from airborne contaminants.

Note: MY-PCR and FE-PCR are not biological safety cabinets. They are designed to provide process protection only from potential airborne contaminants found in the laboratory space.

MY-PCR WORKSTATIONS FEATURES

1. Class 100 vertical laminar flow air
2. 15-Minute UV light timer
3. Clear polycarbonate construction with chemically impervious polypropylene work surface
4. UV shelf with integrated pipette holder
5. Electrical cord pass-through port

Sash position switch and overlap to prevent UV light exposure

HEPA Filters, Pre-Filters, UV bulbs, additional ports and shelves also available.

PR1MA™ Sealing Mats

PR1MA™ 96-Well PCR Sealing Mats are made of silicone, dimensions are 4.67 in. (L) x 2.90 in. (W).

• Pierceable
• Designed for an evaporation-resistant seal to minimize sample loss
• For use in medical, pharmaceutical, food industries, and molecular biology
• Certified free of detectable RNase, DNase, DNA, and PCR inhibitors

PR1MA™ Taq

• Ideal for routine PCR applications as well as genotyping, colony PCR, and fast PCR
• Improved template affinity and solubility for higher enzyme activity and greater yields
• Proprietary buffer optimized for a variety of assay conditions
• Conveniently supplied as a 2X Master Mix or in a 2-tube format of polymerase and separate 5X buffer

PR1MA™ Taq provides superior results for routine applications. Modified to improve DNA-binding, this polymerase offers higher solubility and greater template affinity, resulting in consistently superior performance. PR1MA™ Taq Polymerase exhibits a 5' to 3' nuclease activity, but no 3' to 5' (proofreading) activity and works well with a wide range of DNA templates including GC-rich sequences.

The polymerase is supplied with a 5X buffer containing MgCl2 and a proprietary mix of enhancers (dNTP's not included). For convenience, PR1MA Taq is also available in a ready-to-use 2X Master Mix - just add primers and template DNA. Our "Red Dye" Master Mix incorporates a red-loading dye that allows amplified samples to be loaded directly on an agarose gel.  The red color aids in visualization during pipetting, and higher density of the buffer ensures that the samples will drop into the gel wells.

LabNet LabValues Promotions 2024!

For a limited time enjoy discounted pricing on the MultiGene™ OptiMax Gradient Thermal Cycler (TC9610)

 See flyer for more details.

Promo is valid 07/1/24 – 12/31/24.

Not to be combined with any offers or discounts. No institutional pricing. All terms apply.   

 

MultiGene™ OptiMax Thermal Cycler

This simple-to-program PCR thermal cycler unit is compact in design and built to perform. Programming is intuitive with the large display and multiple pre-programmed templates supplied with the unit.

The OptiMax comes with standard built-in 2-step, temperature optimization, touchdown, and time increment protocols that are easy to adjust to meet your cycling needs. You can select lid temperature or turn it off depending on your needs. The faster ramp rates allow more work to be done in a workday.
 

Additional Thermal Cycler Product Features:

• Unique Flexible Programming with FlexTemp™ technology
• Fast ramp rates 5°C heating / 3.5°C cooling
• Simple user interface
• No condensation after overnight cooling at 4°C
• 3-Year Warranty

Distinct Heat Optimization

The MultiGene™ OptiMax employs a new protocol optimization process improving on older gradient features. This “Better than Gradient” capability allows users to select up to 6 distinct annealing temperatures to be run simultaneously. This is ideal for method optimization as well as applications like genotyping.

The OptiMax utilizes FlexTemp™ technology that effectively separates the 96-well plate into 6 – (4 x 4) temperature regions that allow you to pick the temperatures you want to test against. These regions are easily identified by the blue and black squares visible on the microplate platen. Instead of having the thermal cycler choose temperatures for you, you can choose any temperatures you want within a 24°C range.

The MultiGene™ OptiMax has a heating rate of 5°C /sec and a cooling rate of 3.5°C/sec. We have minimized overshooting and undershooting so that your reactions only see the temperatures they are supposed to see. This also leads to faster runs time.

Intuitive User Interface

The MultiGene™ OptiMax utilizes an intuitive user interface. This user interface is friendly to the laboratory environment and consistent with what our customers previously enjoyed in our earlier models. It can be used with lab gloves even if wet. We have improved the ramping speed and eliminated overshooting and undershooting which contributes to longer run times.

Fully Adjustable Heated Lid

The OptiMax’s heated lid is fully adjustable to provide optimum pressure for use with a broad range of tubes and microplates. For optimum performance, the lid can also be programmed to hold different temperatures between 60° to 65°C and 100° to 115°C. The OptiMax lid prevents condensation and slides away and back from the user to provide full access to samples and reduce the risk of coming in contact with a hot surface.

Specifications 

96-Well PCR Rack

• 96-well, 8 x 12 format
• Holds 0.2ml PCR tubes, strip tubes, and plates (does not fit full skirted plates)
• Supplied with clear lift-off lids

PR1MA™ Untreated Multi-Well Plates

• Available in 6-, 12-, 24-, 48-, and 96-well format

• Polystyrene, NOT Tissue Culture Treated (suspension culture)

• Sterile (gamma irradiated)

• Plates are labeled with alpha-numeric markings for easy identification of individual wells

• Each well has a raised rim that nests into the rings on the single-direction lid to reduce evaporation and limit the risk of cross-contamination

• Lids have a stacking feature for added stability when handling tall stacks of plates 

IsoFreeze® PCR Color-Change Racks

IsoFreeze PCR and IsoFreeze PCR SBS are innovative, high-performance, personal chiller racks. Using proven IsoFreeze temperature maintenance technology, the filled racks maintain a cool (refrigerator) environment on the bench or the workstation platform. This helps maintain a stable environment for PCR reagents, cells, enzymes, or other temperature-sensitive reagents. Industry Standard format tubes, strips, and 96 well plates, in both standard and low profile well shapes, are accommodated and are efficiently cooled in PCR, QPCR, and many other applications.

IsoFreeze PCR Racks are manufactured using polypropylene containing temperature-dependent, color-change dye giving at-a-glance temperature indication for each well. There is a dramatic change to a contrasting color at 7ºC. IsoFreeze gel technology means the wells will stay cool for up to 4 hours, depending on ambient temperature and other factors.

• Available in 2 color changing formats
• Material: Rugged polypropylene and inert gel
• Holds 0.1 or 0.2 mL tubes, strip tubes, and plates up to 96 - well
• Maintains temperature of less than 4°C for up to 2.5 or 3 hours depending on rack 

Specifications

Need an order expedited? Contact your sales rep or email Tech@midsci.com

Looking for mats? Click here! Looking for sealing film? Click here!

Deep Well Plates and Combs for KingFisher Systems 

FEATURING:

Ultra-flat surfaces
Alphanumeric grid reference
Raised Well Design - improved heat sealing and reduced cross contamination
Storage down to -80°C
Automation compliant footprint (SBS/ ANSI/SLAS specification)
Made from Virgin Polypropylene; no mold release agents or regrind
RNase, DNase, DNA, and Pyrogen Free Certification

MID-KF05US-9-N

Our 250 ul 96-well elution plate is ideal for magnetic bead-based nucleic acid and protein affinity purification performed on automated separation systems.  The unique square top with round bottom design allows for optimum mixing and enhanced recovery of magnetic beads.  Wells also have a conical V-shaped bottom to help concentrate precipitates.

Maximum Volume:         350 ul
Working Volume:            250 ul
Volume with Mat:          250 ul
Matching silicone cap mats: MID-MATS-9-PRT, MID-MATS-9-BULK, MID-SQ-9-PRT

MID-KF10US-24-N

Our 5.0 mL 24-well plate is ideal for magnetic bead-based nucleic acid and protein affinity purification (of larger sample sizes, 5 mL) performed on automated separation systems.  The unique square top with round bottom design allows for optimum mixing and enhanced recovery of magnetic beads.  Wells also have a conical V-shaped bottom to help concentrate precipitates.

Maximum Volume:         10.0 mL
Working Volume:            5.0 mL
Volume with Mat:          5.0 mL
Matching silicone cap mats: MID-MATS-24-N

MID-KF22US-9-N

Our 2.0 mL 96-well plate is ideal for magnetic bead-based nucleic acid and protein affinity purification performed on automated separation systems.  The unique square top with round bottom design allows for optimum mixing and enhanced recovery of magnetic beads.  Wells also have a conical V-shaped bottom to help concentrate precipitates.

Maximum Volume:         2.0mL
Working Volume:            1.0mL
Volume with Mat:          1.0mL

Research Essentials 2024 Promo!

For a limited time, buy 2 get 1 free OptiWell™ Deep Well Plates.

Must be the same items only. Enter Item # plus “-VF” for free product.

 

See flyer for details.

 

All promotions are valid while supplies last. Prices are subject to change without notice.

Valid 09/01/24 – 12/31/24. Use Promo Code #RE2024
 

OptiWell™ Deep Well Plates - 384

Available in over 25 different types, The OptiWell™ line of Deep Well Plates is compatible with all popular automated research platforms and robotic equipment. These plates are useful for bacterial cultures, centrifugation (4000xg max RCF), and general sample storage. They can be used with silicone sealing mats and various types of sealing film. All plates are produced in a class 100,000 cleanroom facility and are ANSI / SLAS 1-2004 compliant. Although all OptiWell™ plates are autoclavable, several types are also available presterilized by gamma irradiation in accordance with ISO 13485. All plates are certified RNase/DNase free and non-pyrogenic.

• 24-Well, 48-well, 96-well, and 384-well configurations available
• For automated research platforms / robotic equipment
• Minimal binding/liquid retention
• Black printed alpha-numerics
• Virgin, medical-grade polypropylene
• Conical V-Bottom configurations

 
PR1MA™ Taq Plus

• Higher fidelity for long PCR amplicons, up to 35 kb
• Ideal for problematic templates
• Better sensitivity and higher activity for low-copy and long PCR
• Enzyme of choice for TA cloning

PR1MA™ Taq Plus, an optimized blend of our Hot Start Taq and a proofreading polymerase, provides 5X better fidelity than wild-type Taq and increased enzyme activity. It is the perfect choice for GC and AT rich templates, low copy number, and long PCR.

PR1MA™ Taq Start Taq DNA Polymerase 

PR1MA™ Taq Plus Master Mix, 2X Concentration

TAKE ADVANTAGE OF THIS SPECIAL PRICING! 

Azure Cielo 6 qPCR (AIQ060) PROMOTIONAL PRICE: $16,895
 

*Includes: Azure Cielo 6, analysis software, and 18 months of warranty.

See flyer for more details. 

 

Promo is extended! Now valid 12/31/24. Not to be combined with any offers or discounts. No institutional pricing. All terms apply.

Azure Cielo 3 & 6

The Azure Cielo Real-Time PCR system provide accuracy and sensitivity needed for research, with intuitive touch screen software.

Product Features

• Flexibility:  Engineered for a wide variety of qPCR applications, with high sensitivity and wide dynamic range.

• Intelligent workflow: The user interface is a simple touch screen assay setup.  Protocols can be saved to make assay set up easy and reproducible.

• Reliability: Cielo's optics and thermal block are designed to deliver the same uniformity and reproducibility over at least 1,000 qPCR experiments, giving you confidence in the reliability of the instrument.

• Connectivity: An external PC is not required to run the instrument.

• Data Transfer: This can be performed via Ethernet, Wi-Fi or USB.

Operation - A standalone qPCR system with 10.3” touchscreen interface
Optics - 3 or 6 dye channel filters with LED based excitation for multiplexing
Speed - Fiber optic detection system scans 16 wells simultaneously
Durability - Tested reliability for over 1000 continuous runs
Connectivity - Easily export data via Wi-Fi, ethernet, USB or LAN
Capacity - 96-well format for semi-skirted or un-skirted plates and tubes
Thermal Performance - Marlow Peltier based block with 12 temperature gradient zones, ramp rates up to 6.0°C/sec
Chemistry - Supports reaction volumes between 10 µl–150 µl, open source chemistry, including FAST ~20 minute run-times
Sensitivity - Detect 2-fold differences in target quantities in singleplex reactions and up to 1 copy of amplified DNA, 10 logs of dynamic range

Competitor Q uses a single light source to excite all samples.

Differences in light path length lead to differences in excitation, which requires normalization. Though Competitor Q has said that they do not require normalization dyes, this is not reflected in the data as the data CV values for replicates is significantly higher.

Why doesn’t the Cielo require ROX dye?

 The Cielo does not require ROX dye because it uses in-well detection technology. The light path for excitation and reading emission are collimated directly into the well. As such, the light path length is always the same for each well, so there is no need to normalize for varying light path length. The data from the Cielo is consistently uniform and reproducible.

 

 
PR1MA™ qMAX™ Probe qPCR Mix

• Same high efficiency for multiplex and singleplex reactions
• Includes PR1MA Hot Start Taq Polymerase for greater specificity and accuracy
• Compatible with popular hydrolysis and beacon probes
• Ready to use 2X mastermix
• Early Ct values and detection across a broad dynamic range

Optimized for use with TaqMan™, Scorpions^® and molecular beacon probes, qMax Probe qPCR Mix is a ready-to-use formulation for real-time quantitative assays. qMax Probe utilizes PR1MA™ Hot Start Taq Polymerase or robust PCR with a variety of templates. A specially formulated buffer provides optimal conditions for both superior polymerase function and probe detection, resulting in earlier Ct values and a broad detection range. Complicated, multiplex reactions can be performed without any loss in performance or decrease in detection. The 2X mix requires little, if any optimization and can be used with both fast and standard protocols.

PR1MA™ qMax Probe qPCR Mix can be used to detect any DNA template, including genomic DNA and cDNA. Available in high, low and no ROX formulations, qMax Probe is compatible with most qPCR instruments.

*Please note these products ship on dry ice.  Appropriate shipping charges apply unless otherwise noted on a quote.