IsoFreeze PCR and IsoFreeze PCR SBS are innovative, high performance, personal chiller racks. Using proven IsoFreeze temperature maintenance technology, the filled racks maintain a cool(refrigerator) environment on the bench or on the workstation platform. IsoFreeze gives a chilled and stable environment for PCR reagents, cells, enzymes or other temperature sensitive reagents.

Industry Standard format tubes, strips and 96 well plates, in both standard and low profile well shape, are accommodated and are efficiently cooled in PCR, QPCR and many other applications.

IsoFreeze PCR Racks are manufactured using polypropylene containing novel, temperature dependent color-change dye giving simple, at-a-glance, temperature indication for each well. There is a dramatic change to a contrasting color at 7C. IsoFreeze gel technology means the wells will stay cool for up to 4 hours (see charts below), depending on ambient temperature and other factors.

IsoFreeze PCR can keep your sample cool for over 4 hours!

IsoFreeze PCR SBS high performance is ideal for your automated systems!

IsoFreeze PCR and IsoFreeze PCR SBS available in 2 color change formats!

Need an order expedited? Contact your sales rep or email Tech@midsci.com

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Deep Well Plates and Combs for KingFisher Systems 

FEATURING:

Ultra-flat surfaces
Alphanumeric grid reference
Raised Well Design - improved heat sealing and reduced cross contamination
Storage down to -80°C
Automation compliant footprint (SBS/ ANSI/SLAS specification)
Made from Virgin Polypropylene; no mold release agents or regrind
RNase, DNase, DNA, and Pyrogen Free Certification

MID-KF05US-9-N

Our 250 ul 96-well elution plate is ideal for magnetic bead-based nucleic acid and protein affinity purification performed on automated separation systems.  The unique square top with round bottom design allows for optimum mixing and enhanced recovery of magnetic beads.  Wells also have a conical V-shaped bottom to help concentrate precipitates.

Maximum Volume:         350 ul
Working Volume:            250 ul
Volume with Mat:          250 ul
Matching silicone cap mats: MID-MATS-9-PRT, MID-MATS-9-BULK, MID-SQ-9-PRT

MID-KF10US-24-N

Our 5.0 mL 24-well plate is ideal for magnetic bead-based nucleic acid and protein affinity purification (of larger sample sizes, 5 mL) performed on automated separation systems.  The unique square top with round bottom design allows for optimum mixing and enhanced recovery of magnetic beads.  Wells also have a conical V-shaped bottom to help concentrate precipitates.

Maximum Volume:         10.0 mL
Working Volume:            5.0 mL
Volume with Mat:          5.0 mL
Matching silicone cap mats: MID-MATS-24-N

MID-KF22US-9-N

Our 2.0 mL 96-well plate is ideal for magnetic bead-based nucleic acid and protein affinity purification performed on automated separation systems.  The unique square top with round bottom design allows for optimum mixing and enhanced recovery of magnetic beads.  Wells also have a conical V-shaped bottom to help concentrate precipitates.

Maximum Volume:         2.0mL
Working Volume:            1.0mL
Volume with Mat:          1.0mL

 
PR1MA™ Taq Plus

• Higher fidelity for long PCR amplicons, up to 35 kb
• Ideal for problematic templates
• Better sensitivity and higher activity for low-copy and long PCR
• Enzyme of choice for TA cloning

PR1MA™ Taq Plus, an optimized blend of our Hot Start Taq and a proofreading polymerase, provides 5X better fidelity than wild-type Taq and increased enzyme activity. It is the perfect choice for GC and AT rich templates, low copy number, and long PCR.

PR1MA™ Taq Start Taq DNA Polymerase 

PR1MA™ Taq Plus Master Mix, 2X Concentration

TAKE ADVANTAGE OF THIS SPECIAL PRICING! 

Azure Cielo 6 qPCR (AIQ060) PROMOTIONAL PRICE: $16,895
 

*Includes: Azure Cielo 6, analysis software, and 18 months of warranty.

 

Promo is extended! Now valid 04/01/24 - 06/30/24. Not to be combined with any offers or discounts. No institutional pricing. All terms apply.

Azure Cielo 3 & 6

The Azure Cielo Real-Time PCR system provide accuracy and sensitivity needed for research, with intuitive touch screen software.

Product Features

• Flexibility:  Engineered for a wide variety of qPCR applications, with high sensitivity and wide dynamic range.

• Intelligent workflow: The user interface is a simple touch screen assay setup.  Protocols can be saved to make assay set up easy and reproducible.

• Reliability: Cielo's optics and thermal block are designed to deliver the same uniformity and reproducibility over at least 1,000 qPCR experiments, giving you confidence in the reliability of the instrument.

• Connectivity: An external PC is not required to run the instrument.

• Data Transfer: This can be performed via Ethernet, Wi-Fi or USB.

Operation - A standalone qPCR system with 10.3” touchscreen interface
Optics - 3 or 6 dye channel filters with LED based excitation for multiplexing
Speed - Fiber optic detection system scans 16 wells simultaneously
Durability - Tested reliability for over 1000 continuous runs
Connectivity - Easily export data via Wi-Fi, ethernet, USB or LAN
Capacity - 96-well format for semi-skirted or un-skirted plates and tubes
Thermal Performance - Marlow Peltier based block with 12 temperature gradient zones, ramp rates up to 6.0°C/sec
Chemistry - Supports reaction volumes between 10 µl–150 µl, open source chemistry, including FAST ~20 minute run-times
Sensitivity - Detect 2-fold differences in target quantities in singleplex reactions and up to 1 copy of amplified DNA, 10 logs of dynamic range

Competitor Q uses a single light source to excite all samples.

Differences in light path length lead to differences in excitation, which requires normalization. Though Competitor Q has said that they do not require normalization dyes, this is not reflected in the data as the data CV values for replicates is significantly higher.

Why doesn’t the Cielo require ROX dye?

 The Cielo does not require ROX dye because it uses in-well detection technology. The light path for excitation and reading emission are collimated directly into the well. As such, the light path length is always the same for each well, so there is no need to normalize for varying light path length. The data from the Cielo is consistently uniform and reproducible.

 

 
PR1MA™ qMAX™ Probe qPCR Mix

• Same high efficiency for multiplex and singleplex reactions
• Includes PR1MA Hot Start Taq Polymerase for greater specificity and accuracy
• Compatible with popular hydrolysis and beacon probes
• Ready to use 2X mastermix
• Early Ct values and detection across a broad dynamic range

Optimized for use with TaqMan™, Scorpions^® and molecular beacon probes, qMax Probe qPCR Mix is a ready-to-use formulation for real-time quantitative assays. qMax Probe utilizes PR1MA™ Hot Start Taq Polymerase or robust PCR with a variety of templates. A specially formulated buffer provides optimal conditions for both superior polymerase function and probe detection, resulting in earlier Ct values and a broad detection range. Complicated, multiplex reactions can be performed without any loss in performance or decrease in detection. The 2X mix requires little, if any optimization and can be used with both fast and standard protocols.

PR1MA™ qMax Probe qPCR Mix can be used to detect any DNA template, including genomic DNA and cDNA. Available in high, low and no ROX formulations, qMax Probe is compatible with most qPCR instruments.

*Please note these products ship on dry ice.  Appropriate shipping charges apply unless otherwise noted on a quote.

• Designed for convenience & versatility, this rack can be used while you are working or as a storage rack.
• It includes a lid and can accomodate 32 x 0.2 ml PCR tubes, 24 x 1.5/2.0 ml & 16 x 0.5 ml microtubes.
• Tube wells are alpha-numerically matrixed with raised bold type for quick identification.
  
• If you use cycle plates, 8 or 12 tube strips, or lage quantities of 0.2 ml PCR tubes, the WRI-4A is for you.
• Four retainer clips hold the tray firmly in place, yet allow fast and convenient removal when necessary.
• Available with a clear lid and five flourescent colors: blue, green, orange, pink, yellow, or assorted.

MyGo Mini S

• Small format 16 x 0.1 mL QPCR System with Taqman Multiplexing at an amazing price.
• Amazing accuracy and easy to use software that allows you to control 100s of systems with one PC and analyze the data at the same time.  Windows, Linux and Mac OSX
• Solid state reliability with up to 5-year warranty

Precision and Performance
The MyGo Mini demonstrates superior intra-run and inter-run analytical precision in DNA quantification and melting point analysis. Users can now address biological phenomena with subtle effects on gene expression, or pathogen levels, for example discriminating 10% differences in transcript concentrations. For users performing HRM-based analysis of genetic variants, the combination of thermal control, optical data quality and HRM data analysis of the MyGo Mini S system provide compelling functionality. The system can discriminate all classes of SNP, including Class 4 SNPs via HRM.

Users can now address biological phenomena with subtle effects on gene expression, or pathogen levels, for example discriminating 10% differences in transcript concentrations.

For users performing HRM-based. analysis of genetic variants, the combination of thermal control, optical data quality and HRM data analysis of the MyGo Mini system provide compelling functionality. The system can discriminate all classes of SNP, including Class 4 SNPs via HRM.
 

Easy Analysis
The MyGo Mini system comes with easy-to-use software, and robust, high performance algorithms which provide accurate results from complex data. Automated analysis of complex HRM data makes this powerful technique more accessible to non-experts, and minimizes operator-dependent variability in data analysis. Automated report generation makes life easier for service providers, and users operating within GLP quality systems.

Automated report generation makes life easier for service providers, and users operating within GLP quality systems.

MyGo Pro ESR

The MyGo Pro real-time PCR instrument provides unmatched performance in a convenient format. Novel Full Spectrum Optics deliver 120 optical channels of fluorescence data from every tube in parallel, with no moving parts, for reliable multiplex PCR.

Precision And Performance

The MyGo Pro demonstrates superior intra-run and inter-run analytical precision in DNA quantification and melting point analysis.

Users can now address biological phenomena with subtle effects on gene expression, or pathogen levels, for example discriminating 10% differences in transcript concentrations.

For users performing HRM-based analysis of genetic variants, the combination of thermal control, optical data quality, and HRM data analysis of the MyGo Pro system provides compelling functionality. The system can discriminate all classes of SNP, including Class 4 SNPs via HRM.

Multiplex With Full Spectrum Optics

Novel Full Spectrum Optics provide 120 channels of wavelength-dependent optical data from every well every time. No other qPCR system offers this number of optical channels.

Full Spectrum Optics, combined with advanced matrix deconvolution technology, enable the simultaneous analysis of at least 7 different targets in a single reaction, each monitored with a different color of fluorescent label.

The MyGo Pro comes pre-calibrated for 20 different fluorescent labels, and the software enables users to use any dye with an emission maximum between 510nm and 750nm. No other qPCR system provides the user with this level of flexibility.

Easy Analysis

The MyGo Pro system comes with easy-to-use software, and robust, high-performance algorithms provide accurate results from complex data.

Automated analysis of complex HRM data makes this powerful technique more accessible to non-experts and minimizes operator-dependent variability in data analysis.

Automated report generation makes life easier for service providers, and users operating within GLP quality systems.

Reliability

The MyGo Pro has been designed to last, with very few moving parts, made and tested to exacting standards. The result is a highly reliable instrument, which comes with the option of a 5-year extended warranty.

Speed

With heating rates of 5°C per second and cooling rates of 4°C per second the MyGo Pro is one of the fastest systems available. No other system matches this speed with convenient-to-use disposables. The system demonstrates excellent quantitative precision and speed, with 45 cycles of PCR in 33 minutes. Typical qPCR systems take much longer than this to deliver results.

Compatibility

The MyGo Pro software can be installed on Mac OS X, Windows, and Linux operating systems. Use your computer and your choice of operating system.

Connectivity

The MyGo Pro software does not require a dedicated computer, so users do not need to find the space or money, for an additional computer to run the system. In addition, the software enables the user to control multiple instruments, and simultaneously analyze multiple runs, with one computer.

Our rack is perfect for pre- or post-PCR® sampling applications. Sturdy polypropylene rack accommodates individual 0.2mL tubes or strips of 8 or 12 tubes. Tube wells are easily identified with imprinted numbers and letters. Features a removable hinged lid. Available in natural, blue, green, pink, yellow, or assorted colors (one of each). Packs of 5. PCR® is a registered trademark of Hoffman-LaRoche Inc.

PR1MA™ Mini Gel Electrophoresis System

• Complete system, includes gel box, power supply and casting sets
• Quick and easy gel casting, no tape, clamps or spacers required
• Compact design, footprint: 7 x 5.1 in.

The PR1MA™ Mini Gel electrophoresis system includes all of the equipment that you need to get up and running: gel tank, power supply, and two casting sets. Ideal for personal use, small laboratories, or the classroom, this gel box/power supply combination is compact and easy to use.

The mini gel box attaches directly to the power supply for a secure connection every time. Selectable parameters include 3 preset voltages – 35V, 50V or 100V – and a 99-minute timer. All are set using the membrane keypad.

The safety lid is vented to help dissipate heat generated during electrophoresis and features a viewing area above the gel. Current will only run through the system when the lid is properly in place on top of the gel box.

Trays and casting stands for both 10.5 x 6 cm and 5 x 6 cm gels are included with the PR1MA™ Mini system, as are double-sided combs. The smaller tooth combs are compatible with multi-channel pipettes. Casting is quick and easy – just place the tray in the casting stand and pour. The trays feature a dark contrast strip to help visualize wells while loading and lines that fluoresce under UV and blue light to track the progress of the run.

Specifications