Notice for U.S. Customers – RWD Consumables
Due to changes in U.S.–China tariffs, we will pause sales of these items after Tuesday, April 22, until further notice.
This is in anticipation of the expected De Minimis rule change on May 2.

For more information or alternative product recommendations, please contact us.

RWD Cell Separation Assembly Kit

Applications include immunology research, oncology research, neuroscience, stem cell research, and cell therapy.

RWD magnetic microbead cell separation products consist of Nano-Bead Cell Separation Platform and Nano-Beads (Column Based and Column free). Nano-sized magnetic beads do not need elution, and the separated cells can be directly used in downstream experiments such as flow cytometry, cell culture, single-cell sequencing, and more.

• High purity, high viability, and high recovery rate
• Obtained cells are compatible with downstream applications
• Mild, non-toxic, biodegradable

Kit Includes

• 1 box of LarSep Columns (25/pk)
• 1 LSC Separator (single channel)
• 1 Separation Stand

Work Flow

• Antibody incubation: Incubate the antibody with cell suspension at 4ºC for 10-15 mins.
• Microbead incubation: Incubate the magnetic microbeads with cell suspension at 4ºC for 10-15 mins.
• Magnetic separation: Put the separation column into the magnetic field and add the cell suspension into the column.
• Only cells labeled with magnetic beads will be captured in the column.
• Elution: Remove the column from the magnetic field and add a buffer to elute the positive cells.

Notice for U.S. Customers – RWD Consumables
Due to changes in U.S.–China tariffs, we will pause sales of these items after Tuesday, April 22, until further notice.
This is in anticipation of the expected De Minimis rule change on May 2.

For more information or alternative product recommendations, please contact us.

RWD Human Cell Separation Kits

CD3+ Cell Separation Kit - K1201-10

For positive selection or depletion of CD3+ T cells from peripheral blood, thymus, lymphoid, and tumor tissue.

CD4+ Cell Separation Kit - K1202-10

For positive selection or depletion of T helper cells (Th) from human peripheral blood, umbilical cord blood, lymph nodes, thymus, spleen, skin, and other samples.

CD8+ Cell Separation Kit - K1203-10

For positive selection or depletion of cytotoxic T cells (Tc) from human peripheral blood, umbilical cord blood, lymph nodes, thymus, spleen, skin, and other samples.

CD14+ Cell Separation Kit - K1204-10

For positive selection or depletion of monocytes from human peripheral blood, umbilical cord blood, spleen, lymph nodes, and other samples.

CD45+ Cell Separation Kit - K1205-10

For positive selection or depletion of white blood cells from human peripheral blood and lymphoid tissue samples.

CD19+ Cell Separation Kit - K1206-10

For positive selection or depletion of B cells from human peripheral blood, lymph nodes, bone marrow, and other single-cell suspensions.

Human NK Cell Isolation Kit - K1207-10

For negative selection or depletion of NK cells from single cell suspension of human peripheral blood. Non-NK cells, i.e., T cells, B cells, stem cells, dendritic cells, monocytes, granulocytes, and red blood cells are labeled by biotin antibodies coupled to magnetic beads. High purity NK cells are obtained by isolation of magnetic bead labeled cells.

*All kits are for research use only.

Specifications
 

Item #	Cell Type	Size	Capacity
K1201-10	T Cells	2 x 1 mL	For 1 x 10^9 Total Cells
K1202-10	T Helper Cells	2 x 1 mL
K1203-10	Cytotoxic T Cells	2 x 1 mL
K1204-10	Monocytes	2 x 1 mL
K1205-10	Leukocytes	2 x 1 mL
K1206-10	B Cells	2 x 1 mL
K1207-10	NK Cells	2 x 2 mL

 

Notice for U.S. Customers – RWD Consumables
Due to changes in U.S.–China tariffs, we will pause sales of these items after Tuesday, April 22, until further notice.
This is in anticipation of the expected De Minimis rule change on May 2.

For more information or alternative product recommendations, please contact us.

RWD High Activity Enzymatic Digestion Kits

Optimized enzymatic hydrolysis formulation for efficient and gentle digestion. Preserves the integrity of surface antigenic epitopes. Store in refrigerators at 4°C or -20°C.

• Targeted optimization of enzymatic hydrolysis formula can ensure high cell viability.
• Preserving cell surface epitopes leads to no effect on downstream experiments.

Notice for U.S. Customers – RWD Consumables
Due to changes in U.S.–China tariffs, we will pause sales of these items after Tuesday, April 22, until further notice.
This is in anticipation of the expected De Minimis rule change on May 2.

For more information or alternative product recommendations, please contact us.   

RWD Mouse and Microglia Cell Separation Kits

Mouse CD3+ Cell Separation Kit - K1301-10

For positive selection or depletion of mature T lymphocytes from mouse lymph nodes, thymus, tumors, and other samples.

Mouse CD4+ Cell Separation Kit - K1302-10

For positive selection or depletion of T helper cells (Th) from mouse lymph nodes, thymus, spleen, skin, and other samples.

Mouse CD8+ Cell Separation Kit - K1303-10

For positive selection or depletion of cytotoxic T cells (Tc) from mouse thymus, lymph nodes, spleen, and other samples.

Mouse CD45+ Cell Separation Kit - K1304-10

For positive selection or depletion of white blood cells from mouse lymphoid tissue or non-lymphoid tissue.

Mouse CD19+ Cell Separation Kit - K1305-10

For positive selection or depletion of B cells from mouse spleen and other samples.

CD11b+ (Microglia) cell separation Kit - K1306-10

For positive selection or depletion of microglia from human, adult mice, or rats brain tissue.

Specifications
 

Item #	Cell Type	Size	Capacity
K1301-10	T Lymphocytes	2 x 1 mL	For 1*10^9 Total Cells
K1302-10	T Helper Cells		For 1*10^9 Total Cells
K1303-10	Cytotoxic T Cells		For 1*10^9 Total Cells
K1304-10	Leukocytes		For 1*10^9 Total Cells
K1305-20	B Cells		For 2*10^9 Total Cells
K1306-10	Myeloid Cells		For 1*10^9 Total Cells

 

This brand is being discontinued and will only be available while supplies last.

Need a great DNA Safe Stain at a great price?
Try PR1MA! Click here to order.

Bullseye DNA SafeStain,10,000x 

• Safest DNA stain by far
• Replaces EthBr in agarose gel electrophoresis
• Detection of DNA and RNA
• Excitation at 290 nm & 490 nm
• Emission at 530 nm

DNA SafeStain is a new and safe nucleic acid stain for visualization of double-stranded DNA, single-stranded DNA, and RNA in agarose gels. The dyes are developed to replace toxic ethidium bromide (EthBr, a potent mutagen), commonly used in gel electrophoresis for visualization of nucleic acids in agarose gels. DNA SafeStain is non-carcinogenic by the Ames-test. The results are negative in both the mouse marrow chromophilous erthrocyte micronucleus and mouse spermary spermatocyte chromosomal aberration tests.

DNA SafeStain emits green fluorescence when bound to DNA. It has two excitation wavelength peaks when bound to nucleic acid, at 290nm and 490nm and the emission can be detected at 530nm.

Storage:
Store under dark at 4°C or room temperature.

User Instruction: 
DNA SafeStain can be used in the exact same way as EtBr in agarose gel electrophoresis. For example, add 100µl to 1 liter of 1X running buffer (1xTAE or 1x TBE), and use this DNA SafeStain containing buffer to make the agarose gel and run the gel.

Special Notes: 
1. For best results, dilute the DNA SafeStain 1:10,000 in your gel running buffer and use this dye-containing running buffer to make your agarose gel and to run the gel.

2. As is the case with EtBr; add more DNA SafeStain, if brighter DNA bands are desired. To view the results, any conventional DNA gel viewing light box or gel documentation system should work well with the DNA SafeStain; although, using a LED light instead of a UV light source is preferred due to the harmful nature of UV light.

This Product is For Research Use Only

*For the proper disposal of this product, follow University or Company Guidelines.

Bullseye RT 2X Master Mix

• Up to 9kb cDNA synthesis
• Ensures sample to sample consistency
• Large RNA sample volume capacity
• Ready to use

Size: 100 rxns

RT 2X Master Mix is a proprietary, ready-to-use master mix for first-strand cDNA synthesis in a 2X concentration. This optimized reaction mix contains ribonuclease inhibitor, dNTPs, and a balanced concentration for oligo(dT) and random primers. The ribonuclease inhibitor effectively protects RNA template from degradation. The oligo(dT) anneals selectively to the poly(A) tail of mRNAs and the random primers do not require the presence of poly(A) and they are utilized for the transcription of mRNA 5-end regions. The resultant cDNA can be directly used as template in different PCR experiments.

Kit Components

Storage

• Store at -20°C in a frost-free freezer.

Application

• cDNA synthesis
• Construction of cDNA libraries
• Generation of probes for hybridization 

Protocol

1. Thaw RNA templates and all reagents on ice. Mix each solution by vortexing.
2. Assemble the following components in a tube on ice, and mix well:

1. Heat the mixture at 65°C for 5 mins and incubate on ice for at least 1 min.
2. Collect all components by a brief centrifugation and add 1 µl of the EasyScript RTase to the tube.
3. Mix well and collect all the components by a brief centrifugation.
4. Incubate the tube at room temperature for 10 min for annealing.
5. Perform cDNA synthesis by incubating the tube for 50 min at 42°C.
6. Stop the reaction by heating it at 85°C for 5 min.
7. Chill on ice. The newly synthesized first-strand cDNA is ready for immediate downstream applications. 

Bullseye EasyScript™ Reverse Transcriptase

Application

• Synthesis cDNA froma single-stranded RNA or DNA primer extension
• Sequencing dsDNA
• cDNA library
• Template production for use in PCR
• 3'-end labeling of duplex DNA via end-filling reactions

EasyScript™ Reverse Transcriptase is a genetically modified form of Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV). Both the EasyScript™ and EasyScript™ Plus are RNase H deficient (negative). The enzyme is purified from bacteria h o s t as a single holoenzyme of 71 kDa with the capacity of first-strand cDNA synthesis of template up to 9kb and can be used for routine cDNA synthesis.

Kit Components

Storage Buffer
 
50 mM Tris-HCl (pH 8.3), 100 mM NaCl, 0.1 mM EDTA, 5 mM DTT, 0.1% (v/v) Triton X-100, and 50% (v/v) glycerol.

Storage
 
Store at -20°C in a frost-free freezer. Multiple freezing and thawing of RNA should be avoided. Keep RNA on ice all the time. It is recommended that the first strand cDNA synthesis is carried out under conditions where RNase contamination has been eliminated.

General Protocol

RT-PCR reactions should be assembled in a RNA-free environment. The use of "clean", automatic pipettes designated for PCR and aerosol resistant barrier tips are recommended.

1. Thaw template RNA and all reagents on ice. Mix each solution by vortexing, and centrifuge briefly to collect residual liquid from the sides of the tubes. 
2. Prepare the following reaction mixture in a PCR tube on ice:

3. Incubate at 25°C for 10 minutes if random primer is used. Omit this step if Oligo(dT) primer or sequence specific primer are used.
4. Incubate the mixture at 42°C for 60 minutes.
5. Stop the reaction by heating at 85°C for 5 minutes. 
6. Chill on ice. The newly synthesized first-strand cDNA now can be used directly for PCR amplification.

Notes:
1. Isolation of poly(A)+ RNA from total RNA is not mandatory; however, doing so may improve the yield and purity of the final product. 
2. RNA sample must be free of contaminating genomic DNA.
3. Unlike the oligo(dT) priming, which usually requires no optimization, the ratio of a randomprimer to RNA is critical in terms of the average length of cDNA synthesized in the reaction. Increasing the ratio of random primer/RNA will result in higher yield of shorter (~500 bp) cDNA, whereas decreasing this ratio will produce longer products.

4. The synthesized cDNA should be stored at -20°C.

 Bullseye Individual dNTP's

• Ready-to-use molecular grade dNTP solution for use in DNA polymerization, DNA labeling and sequencing processes
• High purity: >98% by HPLC
• Supplied in solution at pH 7.5
• dNTPs are stable at -20°C, avoid multiple freeze/thawing (For long-term usage, aliquoting is recommended)
• Functionally tested with thermostable polymerases

• Mix of dATP, dCTP, dGTP, dTTP
• Each nucleotide is at a concentration of 100 mM
• Ready-to-use molecular grade dNTP solution for use in DNA polymerization, DNA labelling and sequencing processes
• High purity: > 98% by HPLC
• Supplied in solution at pH 7.5
• dNTPs are stable at - 20°C, avoid multiple freeze/thawing (For long-term usage, aliquoting is recommended)
• Functionally tested with thermostable polymerases 

dNTP SET

(dATP, dCTP, dGTP, dTTP)

100 mM

This brand is being discontinued and will only be available while supplies last.
 

Need a great DNA Safe Stain at a great price?
Try PR1MA! Click here to order.
 

Bullseye DNA Safe Stain Plus

• Higher sensitivity
• Use in the same way as EtBr in agarose gel electrophoresis
• Detection of DNA and RNA
• Safest DNA stain by far
• Low cost
• 10,000X
• Excitation wavelengths at 290nm and 490nm

DNA SafeStain Plus is a highly sensitive green fluorescent DNA/RNA staining reagent for detecting nucleic acids in agarose and polyacrylamide gels. This unique stain gives high sensitivity for detection of double-stranded or single-stranded DNA and RNA. Gels can be post-stained or the stain can be added to gels during gel casting or to the gel running buffer. DNA SafeStain Plus has two excitation wavelength peaks at about 290nm and 490nm, and an emission wavelength at 530nm, making it compatible with a standard UV light box, a blue-light transilluminator, or a gel reader equipped with visible light excitation; such as, a 488 nm laser-based gel scanner.

DNA SafeStain Plus is in a 10,000X concentrated format that can be easily diluted 10,000 times for use in precast gel staining, or 5,000 times for use in post gel staining.

• Pre-optimized ready-to-use PCR reagents
• Less pipetting to avoid contamination
• Quick and easy to set up
• Direct loading for electrophoresis
• Reproducible results

Users only need to add templates and primers, and water if needed. Extra 25 mM magnesium solution is also provided for additional fine adjustments. The Red Mixes contain a non-hazardous inert red dye. The purple mixes contain a non-hazardous inert purple dye. Completed PCR reactions utilizing the D124-R (red dye) or D124-P (purple dye) can be directly loaded into a well of agarose gel or other gels, for electrophoresis. No extra loading buffer is needed.

Storage: 4°C for up to one month, or -20°C for long term storage.

Magnesium Chloride: In general, 1.5 mM MgCl2 is recommended; this may vary with different conditions and primer sets. Some primers/templates may require adjustments for MgCl2 concentration, which can be achieved as shown below:

Directions for use: For a 50 uL reaction, use 25 uL of the Taq Master Mix, add template, primers, and water to a final volume of 50 uL. Cycling conditions vary for different templates and primers. To start with, try 30 cycles as follows: denature at 94°C for 30 seconds, anneal around 55°C for 30 seconds, and extend at 72°C for 1 minute / kb. After the PCR cycles, extend at 72°C for another five minutes to complete the PCR. Then store the reaction at 4°C.

1x Composition: 10 mM KCl, 20 mM Tris HCl (pH 9.0), 16 mM (NH4)2SO4, 0.1% Triton X-100, 1.5 mM MgCl2, 200 mM dNTPs, 2.5 units / 25 uL of Taq DNA polymerase, (optional: trace amount of red or purple dye) and enzyme stabilizers.
 

2x Master Mix Kit (1.5 mM MgCl2) 

Bullseye Taq DNA Polymerase Mix is a ready-to-use 2x reaction mix. Simply add primers, template, and water to successfully carry out primer extensions and other molecular biology applications.

Bullseye Taq polymerase, the NH4+ buffer system, dNTPs, and magnesium chloride are conveniently present in the Taq DNA Polymerase Mix. (Inert Red Dye is present in BE180303 only)

Bullseye Taq DNA Polymerase Mix offers several advantages. Set up time is significantly reduced. The chance of contaminating component stocks is eliminated. Reduction of reagent handling steps leads to better reproducibility. Standard tests can be set up with the confidence that results will be consistent every time.

Composition of 2x Taq Master Mix

150 mM Tris-HCl pH 8.5, 40 mM (NH4)2S04, 3 mM MgCl2, 0.2% Tween 20Ò

4 mM dNTPs

2 units/µL AS ONE Taq polymerase

Inert Red Dye & Stabilizer (BE180303 only)