PR1MA™ T4 Gene 32 Protein

PR1MA™ T4 Gene 32 Protein (T4 gp32) is a single-stranded DNA binding (ssDNA) protein required for E. coli bacteriophage T4 replication. It binds and stabilizes ssDNA structures which facilitates electron microscopic examination, and has also been shown to improve restriction digests, improve T4 DNA polymerase activity, and increase the yield of PCR reactions, including those with long amplicons. 

• Optimal temperature: 37°C
• Heat inactivation: 65°C for 20 minutes
• Storage temperature: -20°C
• 10X T4 gp32 reaction buffer included

Buffer composition

• 50% glycerol
• 50 mM Tris-HCl
• 50 mM KCl
• 1 mM DTT
• 0.1  mM EDTA
• 0.1% Tween-20
• pH = 7.5 

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established. 
 

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PR1MA™ MS2 Phage

PR1MA™ MS2 is an E. coli bacteriophage with a single-stranded RNA genome of 3569 nucleotides protected from nuclease degradation by a capsid of 180 coat protein monomers. This virus is a Biosafety Level 1 organism, not pathogenic to humans. These properties make MS2 phage useful as a process control in nucleic acid-based amplification techniques like RT-PCR, RT-LAMP, particularly those that involve viral RNA extraction.

• Biosafety level 1
• Lysis: 65°C for 20 minutes or by standard RNA extraction
• Storage temperature: 4°C 

Buffer composition

• 10 mM Tris-HCl
• 0.1  mM EDTA
• pH = 8.0 

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.
 

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PR1MA™ RNase Inhibitor

PR1MA™ RNase Inhibitor (RI) is a 50 kDa protein that specifically inhibits RNases A, B, and C by binding noncovalently in a 1:1 ratio at high affinity. It can be used in isothermal amplification and molecular diagnostic assays, cDNA synthesis, and other applications where RNA stability is important. It is ineffective against RNase1, T1, S1 Nuclease, or RNase H. It has no inhibition of polymerase activity when used with Taq DNA polymerase, AMV, M-MuLV, HIV reverse transcriptases, or phage RNA polymerases.

Storage Buffer

• 50% glycerol
• 10 mM Tris-HCl
• 50 mM KCl
• 8 mM DTT
• pH = 7.5 

*To prevent the release of ribonuclease from RNase Inhibitor, temperatures greater than 50°C and high concentrations of denaturing reagents such as urea should be avoided.

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.

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PR1MA™ β - Agarase

PR1MA™ β - Agarase has a high tolerance to inhibitors in electrophoretic buffers such as TBE and TAE, eliminating the need to perform a buffer exchange step before digestion. PR1MA™ β - Agarase has higher thermal stability than other commercially available β - agarases, with a broad range of activity between 42°C and 50°C. Higher temperature digestion reduces the chances of residual agarose gelling at the end of the reaction, resulting in higher recovery of DNA or RNA.

Benefits

• Complete digestion of agarose, with no agarose fragments, left after the digestion
• Obtain DNA or RNA faster - no buffer exchange needed, with direct digestion in TAE or TBE
• Concentration: 1000 u / mL
• Operating temperature: 42°C to 50°C
• Recommended temperature: 50°C
• Storage temperature: -20°C 

Storage Buffer

• 50% glycerol
• 50 mM Tris-HCI
• 50 mM KCI
• 1 mM DTT
• 0.1  mM EDTA
• pH = 7.5 

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.

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Accuris Fast Extraction PCR Kit

The Accuris Fast Extraction PCR Kit features an exclusive single-tube solution that extracts genomic DNA in just 8 minutes. This is swiftly followed by PCR amplification utilizing our High Fidelity Hot Start Red Dye Master Mix, designed for superior endpoint PCR performance. High Fidelity Hot Start Red Dye Master Mix is a 2X formula that incorporates a non-reactive red dye, enabling direct gel loading.

This kit offers versatility to suit different workflows: the PCR-prepared genomic DNA can be directly used in endpoint PCR or can be applied in real-time PCR using either SYBR Green or TaqMan Probe chemistries.

• Offers exceptional versatility and can be used to extract and amplify DNA from a wide range of sample types
• Compatible with animal tissue, plant, saliva, & bacterial samples
• Extraction & Amplification in less than 60 minutes
• Includes High Fidelity Hot Start Red Dye Master Mix to prevent the risk of nonspecific amplification & to visualize bands easily
• Process extracted sample via PCR with included mastermix
• Alternatively, amplify via qPCR (qPCR mastermix not included) 

Each 100 reaction kit includes:

1 tube of High Fidelity Hot Start Red Dye Master Mix (1.25 mL)

1 tube of Fast Extraction Lysis Solution (1.25 mL)

Applications: Extraction & Amplification of Tissue, Plant, Saliva, & Bacteria
 

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PrecisionPak Plant Matter DNA Kit

All-inclusive kit, from lysis to extraction, including protocol, lysis beads, all buffers and reagents, to ensure high quality DNA.

The PrecisionPak Plant Matter DNA extraction kit contains a combination of lysis beads preloaded in sample tubes and lysis buffer for thorough homogenization and high yield. The buffers' formulations are environmentally friendly and do not require ethanol, phenol, chloroform, and isopropanol. The extraction kit is innovatively designed to be faster and more efficient than spin-column methods.

Tough Plant Matter DNA Kit available for: Barley, Beans, Blueberry, Cannabis, Chickpeas, Cloves, Conifer Needles, Corn, Dried Soy Beans, Fungi (M.phaseolina), Ginger Rhizome, Horseradish Root, Leaves, Leek, Malanga (Tuber), Microsporidia, Moss, Mung Beans, Mycelia, N. benthamiana, Pine Nut, Potato, Rice, Soy Bean Plant, Spelt, Sugar Cane Pulp, Sunflower Seeds, Sorghum, Tobacco, Tomato Root, Tomato Seeds, Wheat Berries.

Soft Plant Matter DNA Kit available for: Algae, A thaliana, and Phytoplankton.   
 

PrecisionPak Animal Tissue RNA Kit

All-inclusive kit, from lysis to extraction, including protocol, lysis beads, all buffers and reagents, magnetic extraction beads, and additional reagents to ensure high-quality RNA.

The PrecisionPak Animal Tissue RNA extraction kit contains a combination of lysis beads preloaded in sample tubes and lysis buffer for thorough homogenization and high yield. Magnetic bead technology and additional reagents such as Proteinase K and DNase ensure very high-quality RNA. The magnetic bead technology is innovatively designed to be faster and more efficient than spin-column methods.

Tough Animal Tissue RNA Kits available for: Aorta, Bladder, Cartilage, Cecum, Colon, Diaphragm, Duodenum, Ear, Eye, Feces, Heart, Intestinal Mucosa, Intestine, Jejunum, Larynx, Meconium, Mouse Femur and Tibia, Muscle, Nasal, Olfactory Mucosa, Oropharynx, Placenta, Polyp, Skin, Spinal Cord, Stomach, Tail Snips, Testes, Thalamus, Toe Clip, Tongue, Trachea, Tumor, Umbilical Cord, and Uterus.

Soft Animal Tissue RNA Kit available for: Adipose, Adrenal Gland, Blood, Blood Vessels, Brain, Cerebral Fluid, Gall Bladder, Hypothalamus, Kidney, Liver, Lung, Lymph Node, Marrow, Nerve, Pancreas, Pharynx, Pituitary Gland, Salivary Gland, Spleen, Thymus, and Thyroid.   
 

PrecisionPak Animal Tissue DNA Kit

All-inclusive kit, from lysis to extraction, including protocol, lysis beads, all buffers and reagents, magnetic extraction beads, and additional reagents to ensure high-quality DNA.

The PrecisionPak Animal Tissue DNA extraction kit contains a combination of lysis beads preloaded in sample tubes and lysis buffer for thorough homogenization and high yield. Magnetic bead technology, coupled with additional reagents such as Proteinase K and RNase ensures very high-quality DNA. The magnetic bead technology is innovatively designed to be faster and more efficient than spin-column methods.

Tough Animal Tissue DNA Kit available for: Aorta, Bladder, Cartilage, Cecum, Colon, Diaphragm, Duodenum, Ear, Eye, Feces, Heart, Intestinal Mucosa, Intestine, Jejunum, Larsynx, Meconium, Mouse Femur and Tibia, Muscle, Nasal, Olfactory Mucosa, Oropharynx, Placenta, Polyp, Skin, Spinal Cord, Stomach, Tail Snips, Testes, Thalamus, Toe Clip, Tongue, Trachea, Tumor, Umbilical Cord, and Uterus.

Soft Animal Tissue DNA Kit available for: Adipose, Adrenal Gland, Blood, Blood Vessels, Brain, Cerebral Fluid, Gall Bladder, Hypothalamus, Kidney, Liver, Lung, Lymph Node, Marrow, Nerve, Pancreas, Pharynx, Pituitary Gland, Salivary Gland, Spleen, Thymus, and Thyroid.   
 

PrecisionPak Small Organism DNA Kit

All-inclusive kit, from lysis to extraction, including protocol, lysis beads, all buffers and reagents, magnetic extraction beads, and additional reagents to ensure high quality DNA.

The PrecisionPak Small Organism DNA extraction kit contains a combination of lysis beads preloaded in sample tubes and lysis buffer for thorough homogenization and high yield. Magnetic bead technology, coupled with additional reagents such as Proteinase K and RNase ensure very high-quality DNA. The magnetic bead technology is innovatively designed to be faster and more efficient than spin-column methods.

Tough Small Organism DNA Kit available for: Caterpillar, Crayfish, Crickets, Fruit Fly (Drosophila), Honeybee, Mosquito, Shrimp, Ticks, Zebrafish (D. rerio), Xenopus Tissue.

Soft Small Organism DNA Kits available for: Hookworm, Nematode (B. xylophilus), Roundworm (C. elegans), and Zooplankton.   
 

PrecisionPak Small Organism RNA Kit

All-inclusive kit, from lysis to extraction, including protocol, lysis beads, all buffers and reagents, magnetic extraction beads, and additional reagents to ensure high-quality RNA.

The PrecisionPak Small Organism RNA extraction kit contains a combination of lysis beads preloaded in sample tubes and lysis buffer for thorough homogenization and high yield. Magnetic bead technology, coupled with additional reagents such as Proteinase K and DNase ensures very high-quality RNA. The magnetic bead technology is innovatively designed to be faster and more efficient than spin-column methods.

Tough Small Organism RNA Kits available for: Caterpillar, Crayfish, Crickets, Fruit Fly (Drosophila), Honeybee, Mosquito, Shrimp, Ticks, Zebrafish (D. rerio), Xenopus Tissue.

Soft Small Organism RNA Kit available for: Hookworm, Nematode (B. xylophilus), Roundworm (C. elegans), and Zooplankton.   
 

This brand is being discontinued and will only be available while supplies last.

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Bullseye DNA SafeStain,10,000x 

• Safest DNA stain by far
• Replaces EthBr in agarose gel electrophoresis
• Detection of DNA and RNA
• Excitation at 290 nm & 490 nm
• Emission at 530 nm

DNA SafeStain is a new and safe nucleic acid stain for visualization of double-stranded DNA, single-stranded DNA, and RNA in agarose gels. The dyes are developed to replace toxic ethidium bromide (EthBr, a potent mutagen), commonly used in gel electrophoresis for visualization of nucleic acids in agarose gels. DNA SafeStain is non-carcinogenic by the Ames-test. The results are negative in both the mouse marrow chromophilous erthrocyte micronucleus and mouse spermary spermatocyte chromosomal aberration tests.

DNA SafeStain emits green fluorescence when bound to DNA. It has two excitation wavelength peaks when bound to nucleic acid, at 290nm and 490nm and the emission can be detected at 530nm.

Storage:
Store under dark at 4°C or room temperature.

User Instruction: 
DNA SafeStain can be used in the exact same way as EtBr in agarose gel electrophoresis. For example, add 100µl to 1 liter of 1X running buffer (1xTAE or 1x TBE), and use this DNA SafeStain containing buffer to make the agarose gel and run the gel.

Special Notes: 
1. For best results, dilute the DNA SafeStain 1:10,000 in your gel running buffer and use this dye-containing running buffer to make your agarose gel and to run the gel.

2. As is the case with EtBr; add more DNA SafeStain, if brighter DNA bands are desired. To view the results, any conventional DNA gel viewing light box or gel documentation system should work well with the DNA SafeStain; although, using a LED light instead of a UV light source is preferred due to the harmful nature of UV light.

This Product is For Research Use Only

*For the proper disposal of this product, follow University or Company Guidelines.