• Lyophilized components to ship without cold chain
• Highly specific detection profile and high priming efficiency
• Rapid detection of COVID-19 and does not detect other related coronavirus strains

Design of our COVID-19 was an intricate process using a dedicated team of expert bioinformaticians. Finding the right sequence is critical for not only assay specificity, but also sensitivity which can be inhibited by primer-dimer formation. These ultimately reduce the assay's ability to detect SARS-CoV-2 accurately. However, the right design will mitigate these risks and ensure a specific, sensitive and accurate test, as we have done.

Our surveillance program is in place to track the mutation of the virus. Our expert bioinformaticians analyze daily published virus sequences to ensure our test maintains a 100% detection profile. Each new mutation presents a risk to any COVID-19 kit detection and false negatives without surveillance programs in place. As of the 17th July 2020 our COVID-19 test has a 100% detection profile with the 42,655 full length, good quality SARS-CoV-2 sequences published on the GISAID-EpiCoV database.

Ensuring your COVID-19 test is stable during transit and storage is vital to prevent reagent degradation. Within our COVID-19 genesig® Real Time PCR assay we provide oasig components that are provided freeze dried which stabilizes the active components. This enables the reagents to be shipped at ambient temperature. This simplifies the logistics of shipping and is cost effective. If you're in a sophisticated laboratory or a mobile field hospital we can supply complete qPCR packages to your door quickly and cheaply via standard shipping methods without the need for dry ice or a cold chain of any sort.

The COVID-19 genesig® Real Time PCR assay was granted Emergency Use Authorization Only by the United States Food and Drug Administration (FDA).

• Reduces formation of non-specific products
• Improves results from multiplex reactions
• Essential for low copy number
• Buffer II: potassium/ammonium buffer for multiplex reactions
• Activated at elevated temperatures
• Gives higher specificity and greater yields than standard Taq
• Chemical moiety is attached to the enzyme at the active site and renders it inactive at room temperature; is cleaved during a 15 min. heat activation step
• Prevents mispriming during setup and the first ramp of thermal cycling

This brand is being discontinued.

Looking for other alternatives?

Visit our PR1MA Polymerase page for more options!

Bullseye PREMIUM PR DNA Polymerase

• Provides higher fidelity than standard Taq DNA Polymerase
• Produces blunt-ended fragments
• Processes <3 kb with extremely high fidelity

Store at -20°C. For in-vitro laboratory use only

Bullseye PREMIUM PR DNA Polymerase is a thermostable enzyme with proofreading ability, which can be used in primer extension reactions and other molecular biology applications. PR Polymerase exhibits both 5'-3' DNA polymerase activity and 3'-5' proofreading exonuclease activity. It is recommended for applications, which require extremely high fidelity or blunt ending.

Optimal reaction conditions are achieved by using the 10x Ammonium buffer containing MgCl2 provided with the enzyme. 25 mM MgCl2 is also included separately, in case a higher MgCl2 concentration is required for a specific reaction.

Unit Definition
One unit is defined as the amount that incorporates 10 nmoles of dNTPs into acid-precipitable form in 30 minutes at 72°C under standard assay conditions.

10X Ammonium Reaction Buffer
Tris-HCl pH 8.5, (NH4)2SO4, 1% Tween20,
15mM MgCl2

PR Storage Buffer
50 mM Tris-HCl (pH 8.0), 50 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol, 0.1% NP40, 0.1% Tween-20.

Quality Control
Endonuclease, exonuclease and priming activities are not detected after 3 hours incubation of 1 mg of pUC19 plasmid DNA and 0.5 µg EcoR I digested lambda phage DNA at 72°C in the presence of 40 units of PR DNA Polymerase.

Suggested Protocol using PR DNA Polymerase
 
This protocol serves as a guideline. Optimal reaction conditions must be individually determined.

1. Thaw 10X Ammonium Buffer, dNTP mix, and primer solutions. It is important to mix the solutions completely before use to avoid localized concentrations of salts.

2. Prepare a master mix according to Table 1. The master mix typically contains all the components needed for extension except the template DNA.
The optimal MgCl2 concentration should be determined empirically but in most cases a concentration of 1.5 mM, as provided in the 1X Ammonium Buffer, will produce satisfactory results. Table 2 provides the volume of 25 mM MgCl2 to add to the master mix if a higher MgCl2 concentration is required.

Table 1. Reaction components (master mix and template DNA)

Table 2. MgCl2 concentration in a 50 uL reaction

3. Mix the master mix thoroughly and dispense appropriate volumes into reaction tubes. Mix gently, e.g., by pipetting the master mix up and down a few times.

4. Add template DNA (0.1-0.5 mg/reaction) to the individual tubes containing the master mix.

5. Program the thermal cycler according to the manufacturer's instructions. PR is a proofreading enzyme and requires an extension time of 1-2 min/kb. For maximum yield and specificity, temperatures and cycling times should be optimized for each new template target or primer pair.

Bullseye PREMIUM HS-Taq DNA Polymerase

Store at -20°C. For in-vitro laboratory use only

General Description

Bullseye HS Taq DNA Polymerase is a modified form of Bullseye Taq DNA Polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during setup and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. The result is higher specificity and greater yields when compared to standard DNA polymerases.

Once the reaction reaches optimal activating temperature, the chemical moiety is cleaved during a 15 minute heat activation step, releasing the active HS Taq DNA Polymerase into the reaction.

10X HS Buffer I

Tris-HCl, pH 8.5 (NH4)2S04, 15 mM MgCl2, 1% Tween 20.

10X HS Buffer II

An optimized buffer with a balanced ammonium/potassium concentration. May improve results with more complicated PCR reactions such as multiplex PCR.

Tris-HCl pH 8.7, Balanced KCl/(NH4)2S04, 15 mM MgCl2, 1% Tween 20.

HS Taq Storage Buffer

Enzyme is supplied in 20 mM Tris-HCl pH 8.3, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Tween 20®, 0.5% NP40, 50% glycerol.

Unit Definition

One unit is defined as the amount that incorporates 10 nmoles of dNTPs into acid-precipitable form in 30 minutes at 72°C under standard assay conditions.

Quality Control

Endonuclease, exonuclease and priming activities are not detected after 3 hours incubation of 1 mg of pUC19 plasmid DNA and 0.5 µg EcoR I digested lambda phage DNA at 72°C in the presence of 40 units of TEMPase DNA Polymerase.

Suggested Protocol using HS Taq DNA Polymerase

This protocol serves as a guideline. Optimal reaction conditions must be individually determined.

• 15 mM MgCl2 is present in the 10X HS Buffer I and II. However, in some applications, more than 1.5mM MgCl2 is needed. For this reason, 25mM MgCl2 is included with the kit. Table 2 provides the volume of 25mM MgCl2 to add to the master mix if a higher MgCl2 concentration is required.

  1. Thaw 10X HS Buffer I or/and 10X HS Buffer II, dNTP mix, primer solutions. It is important to mix the solutions completely before use to avoid localized concentrations of salts.

  2. Prepare a master mix according to Table 1. The master mix typically contains all the components needed for extension except the template DNA.

Table 1. Reaction components (master mix & template DNA) 

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Bullseye PREMIUM GC Rich Optimized DNA Polymerases and Kits

Key Features

• Amplification of multiple DNA targets with high GC content
• High specificity, sensitivity and product yield
• Diminished formation of non-specific product
• Detection of low copy number targets

Kit Components

• HS DNA Polymerase in Storage Buffer
• 5 U/ml Hot Start DNA Polymerase, 20 mM Tris-HCl pH 8.9, 100
• mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Tween 20,
• 0.5% NP40, 50% glycerol.
• 4x GC Buffer I
• Optimized buffer components, 6 mM MgCl
• 4x GC Buffer II
• Optimized buffer components, 6 mM MgCl
• MgCl
• 25 mM MgCl in PCR grade water

Bullseye PREMIUM HS-Taq DNA Polymerase Master Mix with HS Buffer I

Store at -20°C. For in-vitro laboratory use only

Bullseye HS Taq Polymerase, 2X Mix is a ready-to-use 2.0X master mix. Simply add primers, template, and water to successfully carry out primer extensions and other molecular biology applications.

Bullseye HS Taq Polymerase Mix, the NH4+ buffer system, dNTPs and magnesium chloride are present in HS Taq Pol Mix with HS Buffer I. Each reaction requires 25 µL of the 2.0X reaction mix. Simply add primers, template and water to a total reaction volume of 50 uL.

Bullseye HS Taq Polymerase Mix is a modified form of Bullseye Taq DNA Polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during setup and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. The result is higher specificity and greater yields when compared to standard DNA polymerases.

Bullseye" HS Taq Polymerase Mix offers several advantages. Set up time is significantly reduced. The chance of contaminating component stocks is eliminated. Reduction of reagent handling steps leads to better reproducibility. Standard tests can be set up with the confidence that results will be consistent every time.

Composition of HS Taq Pol, 2x Mix

Tris-HCl pH 8.5, (NH4)2S04, 3.0mM MgCl2, 0.2% Tween 20Ã, 0.4 mM dNTPs, 0.2 units/uL HS Hot Start DNA Polymerase Stabilizer

Suggested Protocol using HS Taq Pol, 2x Mix

This protocol serves as a guideline only. Optimal reaction conditions may vary and must be individually determined.

• Set up reaction mixtures in an area separate from that used for DNA preparation or product analysis.
• The table below shows the reaction set up for a final volume of 50 mL.
• Important: Mix the solutions completely before use to avoid localized concentrations of salts.

  1. Set up each reaction as follows:

• Excellent all-purpose amplification enzyme
• Thermostable recombinant DNA polymerase from Thermus aquaticus
• Exhibits very high activity in primer extension
• Has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity
• Does not have 3' to 5' exonuclease activity-no proofreading ability
• Leaves an A-overhang, which makes the enzyme ideal for TA cloning
• Includes MgCl² in buffer

This brand is being discontinued, please contact us for other options.

Looking for other alternatives?

Visit our PR1MA Polymerase page for more options!

Bullseye PREMIUM Taq DNA Polymerases

Bullseye Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa.

Bullseye Taq DNA Polymerase has both a 5'®3' DNA polymerase and a 5'®3' exonuclease activity. The enzyme lacks a 3'®5' exonuclease activity (no proofreading ability). Taq DNA Polymerase leaves an A¢ overhang, which makes the enzyme ideal for TA cloning.

The 10X Reaction Buffer provided does not contain Mg+2. 25 mM MgCl2 is supplied separately.

10X Mg++ Free Standard Buffer

100 mM Tris-HCl pH 8.3, 500 mM KCl.

Unit Definition

One unit is defined as the amount that incorporates 10 nmoles of dNTPs into acid-precipitable form in 30 minutes at 72°C under standard assay conditions.

Storage and Dilution Buffer

Enzyme is supplied in 20 mM Tris-HCl pH 8.3, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Tween 20, 0.5&#37;NP40, 50% glycerol.

Quality Control

Each lot of Taq DNA Polymerase is tested for contaminating activities, with no trace of endonuclease activity, nicking activity, exonuclease activity or priming activity.

Suggested Protocol using Taq DNA Polymerase

This protocol serves as a guideline only. Optimal reaction conditions may vary and must be individually determined.

Table 1. Reaction components (master mix & template DNA)

Table 2. MgCl2 concentration in a 50 mL reaction

Table 2 provides the volume of 25 mM MgCl2 to add to the master mix if a certain MgCl2 concentration is required.

This brand is being discontinued.

Need a great DNA Ladders at a great price?

Try our PR1MA™ SmartCheck DNA Ladders!

Bullseye 100bp DNA Ladder RTU (Ready-to-Use)

• A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield 11 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis.
• The DNA includes fragments ranging from 100-1,500 base pairs. The 500 and 1,500 base pair bands have increased intensity to serve as reference points.
• The approximate mass of DNA in each band is provided (0.5 ug a load) for approximating the mass of DNA in comparably intense samples of similar size.

Source:

• PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol and equilibrated to 10nM Tris-HCl (pH 8.0) and 10 mM EDTA.

Range: 100-1,500 bp (DL1); 250-10,000 bp (DL5)
Number of bands: 11 (DL1); 13 (DL5)
Concentration: 100 ug / mL
Package: 50 ug / 500 uL
Recommended Load: 5 uL / well
Contains orange G & xylene cyanol FF as tracking dyes. (DL1)
Contains bromophenol blue & xylene cyanol FF as tracking dyes. (DL5)

Storage:

• Store at 25°C for 6 months
• Store at 4°C for 12 months
• Store at -20°C for 24 months

Bullseye 100bp DNA Ladder

• Ready to use
• Contains 11 DNA bands: 100-1500bp.
• Clearly identifiable 500bp band as reference
• 500ng DNA/6 uL/loading
• Easy to load
• Stable at room temperature
• Supplied with 6x sample loading buffer

Bullseye 100bp DNA Ladder consists of 11 DNA fragments ranging in size from 100-1500 base pairs (bp). 6 uL will yield at least 30 ng DNA in any single band. The intensity of the 500bp band has been increased to serve as a reference for easy identification.

Size: 1200 uL
Storage: Store at -20°C.
Concentration: 500 ng/6 uL

Loading Buffer Composition:

• 10mM Tris-HCl
• 1mM EDTA (pH 8.0)
• 0.02% Bromophenol blue
• 0.02% Xylene cyanol
• 5% Glycerol

Usage: Add at least 6 uL Bullseye 100bp DNA Ladder directly to wells designated for markers. You may need more than 6 uL of ladder, depending on well size and level of intensity needed to visualize the bands.

Please give us a call for a sample.