PR1MA™ Taq DNA Polymerase 1X Master Mix

PR1MA™ DNA polymerase 1X Master Mix saves time and cost by enabling direct PCR amplification of unpurified templates. It contains a recombinant, truncated (lacks 5’ to 3’ exonuclease activity), highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme is thermostable up to 98°C for polymerase chain reaction assays and is provided as a complete reaction master mix consisting of reaction buffer, dNTPs, MgCl2, and loading dye and only requires the addition of primers and DNA template. Once PCR is complete, the reaction products can be loaded directly into an agarose gel for analysis.

• Lacks exonuclease activity
• Thermotolerant up to 98°C
• Inhibitor Resistant
• Ideal for colony PCR, genotyping, and GC-rich templates
• Storage temperature: -20°C 

Important note: Please be sure to use the buffer provided with this product to ensure optimal results.

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.

Proudly made in the USA! Click here for more made in America products!
  

PR1MA™ T4 Gene 32 Protein

PR1MA™ T4 Gene 32 Protein (T4 gp32) is a single-stranded DNA binding (ssDNA) protein required for E. coli bacteriophage T4 replication. It binds and stabilizes ssDNA structures which facilitates electron microscopic examination, and has also been shown to improve restriction digests, improve T4 DNA polymerase activity, and increase the yield of PCR reactions, including those with long amplicons. 

• Optimal temperature: 37°C
• Heat inactivation: 65°C for 20 minutes
• Storage temperature: -20°C
• 10X T4 gp32 reaction buffer included

Buffer composition

• 50% glycerol
• 50 mM Tris-HCl
• 50 mM KCl
• 1 mM DTT
• 0.1  mM EDTA
• 0.1% Tween-20
• pH = 7.5 

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established. 
 

Proudly made in the USA! Click here for more made in America products!
  

PR1MA™ MS2 Phage

PR1MA™ MS2 is an E. coli bacteriophage with a single-stranded RNA genome of 3569 nucleotides protected from nuclease degradation by a capsid of 180 coat protein monomers. This virus is a Biosafety Level 1 organism, not pathogenic to humans. These properties make MS2 phage useful as a process control in nucleic acid-based amplification techniques like RT-PCR, RT-LAMP, particularly those that involve viral RNA extraction.

• Biosafety level 1
• Lysis: 65°C for 20 minutes or by standard RNA extraction
• Storage temperature: 4°C 

Buffer composition

• 10 mM Tris-HCl
• 0.1  mM EDTA
• pH = 8.0 

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.
 

Proudly made in the USA! Click here for more made in America products!
  

PR1MA™ RNase Inhibitor

PR1MA™ RNase Inhibitor (RI) is a 50 kDa protein that specifically inhibits RNases A, B, and C by binding noncovalently in a 1:1 ratio at high affinity. It can be used in isothermal amplification and molecular diagnostic assays, cDNA synthesis, and other applications where RNA stability is important. It is ineffective against RNase1, T1, S1 Nuclease, or RNase H. It has no inhibition of polymerase activity when used with Taq DNA polymerase, AMV, M-MuLV, HIV reverse transcriptases, or phage RNA polymerases.

Storage Buffer

• 50% glycerol
• 10 mM Tris-HCl
• 50 mM KCl
• 8 mM DTT
• pH = 7.5 

*To prevent the release of ribonuclease from RNase Inhibitor, temperatures greater than 50°C and high concentrations of denaturing reagents such as urea should be avoided.

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.

Proudly made in the USA! Click here for more made in America products!
  

PR1MA™ β - Agarase

PR1MA™ β - Agarase has a high tolerance to inhibitors in electrophoretic buffers such as TBE and TAE, eliminating the need to perform a buffer exchange step before digestion. PR1MA™ β - Agarase has higher thermal stability than other commercially available β - agarases, with a broad range of activity between 42°C and 50°C. Higher temperature digestion reduces the chances of residual agarose gelling at the end of the reaction, resulting in higher recovery of DNA or RNA.

Benefits

• Complete digestion of agarose, with no agarose fragments, left after the digestion
• Obtain DNA or RNA faster - no buffer exchange needed, with direct digestion in TAE or TBE
• Concentration: 1000 u / mL
• Operating temperature: 42°C to 50°C
• Recommended temperature: 50°C
• Storage temperature: -20°C 

Storage Buffer

• 50% glycerol
• 50 mM Tris-HCI
• 50 mM KCI
• 1 mM DTT
• 0.1  mM EDTA
• pH = 7.5 

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.

Proudly made in the USA! Click here for more made in America products!
  

Accuris Fast Extraction PCR Kit

The Accuris Fast Extraction PCR Kit features an exclusive single-tube solution that extracts genomic DNA in just 8 minutes. This is swiftly followed by PCR amplification utilizing our High Fidelity Hot Start Red Dye Master Mix, designed for superior endpoint PCR performance. High Fidelity Hot Start Red Dye Master Mix is a 2X formula that incorporates a non-reactive red dye, enabling direct gel loading.

This kit offers versatility to suit different workflows: the PCR-prepared genomic DNA can be directly used in endpoint PCR or can be applied in real-time PCR using either SYBR Green or TaqMan Probe chemistries.

• Offers exceptional versatility and can be used to extract and amplify DNA from a wide range of sample types
• Compatible with animal tissue, plant, saliva, & bacterial samples
• Extraction & Amplification in less than 60 minutes
• Includes High Fidelity Hot Start Red Dye Master Mix to prevent the risk of nonspecific amplification & to visualize bands easily
• Process extracted sample via PCR with included mastermix
• Alternatively, amplify via qPCR (qPCR mastermix not included) 

Each 100 reaction kit includes:

1 tube of High Fidelity Hot Start Red Dye Master Mix (1.25 mL)

1 tube of Fast Extraction Lysis Solution (1.25 mL)

Applications: Extraction & Amplification of Tissue, Plant, Saliva, & Bacteria
 

Proudly made in the USA! Click here for more made in America products!
  

PrecisionPak Plant Matter DNA Kit

All-inclusive kit, from lysis to extraction, including protocol, lysis beads, all buffers and reagents, to ensure high quality DNA.

The PrecisionPak Plant Matter DNA extraction kit contains a combination of lysis beads preloaded in sample tubes and lysis buffer for thorough homogenization and high yield. The buffers' formulations are environmentally friendly and do not require ethanol, phenol, chloroform, and isopropanol. The extraction kit is innovatively designed to be faster and more efficient than spin-column methods.

Tough Plant Matter DNA Kit available for: Barley, Beans, Blueberry, Cannabis, Chickpeas, Cloves, Conifer Needles, Corn, Dried Soy Beans, Fungi (M.phaseolina), Ginger Rhizome, Horseradish Root, Leaves, Leek, Malanga (Tuber), Microsporidia, Moss, Mung Beans, Mycelia, N. benthamiana, Pine Nut, Potato, Rice, Soy Bean Plant, Spelt, Sugar Cane Pulp, Sunflower Seeds, Sorghum, Tobacco, Tomato Root, Tomato Seeds, Wheat Berries.

Soft Plant Matter DNA Kit available for: Algae, A thaliana, and Phytoplankton.   
 

PrecisionPak Animal Tissue RNA Kit

All-inclusive kit, from lysis to extraction, including protocol, lysis beads, all buffers and reagents, magnetic extraction beads, and additional reagents to ensure high-quality RNA.

The PrecisionPak Animal Tissue RNA extraction kit contains a combination of lysis beads preloaded in sample tubes and lysis buffer for thorough homogenization and high yield. Magnetic bead technology and additional reagents such as Proteinase K and DNase ensure very high-quality RNA. The magnetic bead technology is innovatively designed to be faster and more efficient than spin-column methods.

Tough Animal Tissue RNA Kits available for: Aorta, Bladder, Cartilage, Cecum, Colon, Diaphragm, Duodenum, Ear, Eye, Feces, Heart, Intestinal Mucosa, Intestine, Jejunum, Larynx, Meconium, Mouse Femur and Tibia, Muscle, Nasal, Olfactory Mucosa, Oropharynx, Placenta, Polyp, Skin, Spinal Cord, Stomach, Tail Snips, Testes, Thalamus, Toe Clip, Tongue, Trachea, Tumor, Umbilical Cord, and Uterus.

Soft Animal Tissue RNA Kit available for: Adipose, Adrenal Gland, Blood, Blood Vessels, Brain, Cerebral Fluid, Gall Bladder, Hypothalamus, Kidney, Liver, Lung, Lymph Node, Marrow, Nerve, Pancreas, Pharynx, Pituitary Gland, Salivary Gland, Spleen, Thymus, and Thyroid.   
 

PrecisionPak Animal Tissue DNA Kit

All-inclusive kit, from lysis to extraction, including protocol, lysis beads, all buffers and reagents, magnetic extraction beads, and additional reagents to ensure high-quality DNA.

The PrecisionPak Animal Tissue DNA extraction kit contains a combination of lysis beads preloaded in sample tubes and lysis buffer for thorough homogenization and high yield. Magnetic bead technology, coupled with additional reagents such as Proteinase K and RNase ensures very high-quality DNA. The magnetic bead technology is innovatively designed to be faster and more efficient than spin-column methods.

Tough Animal Tissue DNA Kit available for: Aorta, Bladder, Cartilage, Cecum, Colon, Diaphragm, Duodenum, Ear, Eye, Feces, Heart, Intestinal Mucosa, Intestine, Jejunum, Larsynx, Meconium, Mouse Femur and Tibia, Muscle, Nasal, Olfactory Mucosa, Oropharynx, Placenta, Polyp, Skin, Spinal Cord, Stomach, Tail Snips, Testes, Thalamus, Toe Clip, Tongue, Trachea, Tumor, Umbilical Cord, and Uterus.

Soft Animal Tissue DNA Kit available for: Adipose, Adrenal Gland, Blood, Blood Vessels, Brain, Cerebral Fluid, Gall Bladder, Hypothalamus, Kidney, Liver, Lung, Lymph Node, Marrow, Nerve, Pancreas, Pharynx, Pituitary Gland, Salivary Gland, Spleen, Thymus, and Thyroid.   
 

PrecisionPak Small Organism DNA Kit

All-inclusive kit, from lysis to extraction, including protocol, lysis beads, all buffers and reagents, magnetic extraction beads, and additional reagents to ensure high quality DNA.

The PrecisionPak Small Organism DNA extraction kit contains a combination of lysis beads preloaded in sample tubes and lysis buffer for thorough homogenization and high yield. Magnetic bead technology, coupled with additional reagents such as Proteinase K and RNase ensure very high-quality DNA. The magnetic bead technology is innovatively designed to be faster and more efficient than spin-column methods.

Tough Small Organism DNA Kit available for: Caterpillar, Crayfish, Crickets, Fruit Fly (Drosophila), Honeybee, Mosquito, Shrimp, Ticks, Zebrafish (D. rerio), Xenopus Tissue.

Soft Small Organism DNA Kits available for: Hookworm, Nematode (B. xylophilus), Roundworm (C. elegans), and Zooplankton.   
 

PrecisionPak Small Organism RNA Kit

All-inclusive kit, from lysis to extraction, including protocol, lysis beads, all buffers and reagents, magnetic extraction beads, and additional reagents to ensure high-quality RNA.

The PrecisionPak Small Organism RNA extraction kit contains a combination of lysis beads preloaded in sample tubes and lysis buffer for thorough homogenization and high yield. Magnetic bead technology, coupled with additional reagents such as Proteinase K and DNase ensures very high-quality RNA. The magnetic bead technology is innovatively designed to be faster and more efficient than spin-column methods.

Tough Small Organism RNA Kits available for: Caterpillar, Crayfish, Crickets, Fruit Fly (Drosophila), Honeybee, Mosquito, Shrimp, Ticks, Zebrafish (D. rerio), Xenopus Tissue.

Soft Small Organism RNA Kit available for: Hookworm, Nematode (B. xylophilus), Roundworm (C. elegans), and Zooplankton.