PR1MA™ Guanidine Thiocyanate

CAS Number: 593-84-0
Molecular Weight: 118.2
Chemical Formula: CH₅N₃ • HSCN
Solubility: Water
Storage Temp: Room Temperature

Protein denaturant used for purifying proteins and nucleic acids.

Toxicity: LD50

Research or further manufacturing use only, not for food or drug use.

• Appearance: White to slightly off white crystalline powder
• Assay (Argentometric Titration): ≥ 99.0 %
• Assay (Ion Chromatography): ≥ 99.5 %
• Chloride Content: ≤ 1.0 %
• Cobalt (Co) : ≤ 5 ppm
• FTIR: Conforms to reference
• Iron: ≤ 5 ppm
• pH: 5.0 - 7.0
• DNase/ RNase: None Detected
• Melting Point: 118 - 121 ^oC
• Solubility: Pass
• Sulfate Ash: ≤ 0.5 %
• Moisture : ≤ 0.5 %
• Grade: Ultra Pure
• Country of Origin: Report

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PR1MA™ Guanidine Hydrochloride

CAS Number: 50-01-1
Molecular Weight: 95.5
Chemical Formula: CH₅N₃-HCl
Solubility: Water
Storage Temp: Room Temperature

Protein denaturant used for purifying proteins and nucleic acids.

Research or further manufacturing use only, not for food or drug use.

• Appearance: White Crystalline Powder
• Assay (Titration, dried basis): ≥ 99.5 %
• Copper (Cu): ≤ 1 ppm
• DNase, RNase, protease: Not Detected
• Free Acid: ≤ 0.01 %
• Heavy Metals (as Pb): ≤ 0.5 ppm
• Identification (IR): Conforms to Known Structure
• Iron (Fe): ≤ 1 ppm
• Loss on Drying: ≤ 1 %
• Melting Point: 185 - 188 ^oC
• pH (10% solution in H₂O @ 25^oC): 5.0 - 7.0
• Residue on Ignition: ≤ 0.05 %
• Solubility (6% Solution in H₂O): Clear
• UVA (230nm, 6M H₂O solution): ≤ 0.14
• UVA (240nm, 6M H₂O solution): ≤ 0.05
• UVA (260nm, 6M H₂O solution): ≤ 0.03
• Zinc (Zn): ≤ 1 ppm

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PR1MA™ qMAX Green qPCR Mix with Blue Tracking Dye

• Superior sensitivity and fast cycling with exceptional results
• Ideal for low copy number templates
• Early Ct values and detection across a broad dynamic range
• Ready to use 2X Master Mix
• Includes PR1MA™ Hot Start Taq Polymerase for greater specificity and accuracy 

Supplied as a ready-to-use 2X master mix, PR1MA™ qMax Green has been engineered for high sensitivity, fast cycling, and excellent reproducibility. PR1MA™ Hot Start Polymerase provides accurate PCR of a variety of templates including low copy number and difficult sequences, while the proprietary qMax Green intercalating dye exhibits higher fluorescence and lower PCR inhibition than other popular green dyes.

These two components are supported by a specially formulated buffer with an exacting combination of salts, PCR enhancers, stabilizers, and pH that results in earlier Ct values and a high specificity across a broad dynamic range.

*Please note these products ship on dry ice. Appropriate shipping charges apply unless otherwise noted on a quote.

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PR1MA™ Proteinase K

CAS Number: 39450-01-6
Solubility: Water
Storage Temp: -20°C

From Tritirachium album. Serine protease for proteolytic inactivation of nucleases during isolation of DNA and RNA.

Shipping Weight: 0 lbs
Shipping Dimensions: 1.25 x 1.75 x 1.25

Specifications

Appearance: White to Off White Lyophilized Solid
Solubility: 50mM Tris-HCl (pH 7.5), 3mM CaCl2, 50% Glycerol
Purity: ≥ 98.0 %
DNase/ RNase: Not Detected
Activity: ≥ 30.0 U/mg
Unit Definition: 1 unit is defined as the amount of enzyme that will liberate 1.0 µmol of tyrosine from casein/min @37°C, pH 7.5
Country of Origin: China

Research or further manufacturing use only, not for food or drug use.

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PR1MA™ Formamide, Super Pure

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PR1MA™ Sodium Acetate, Anhydrous

CAS Number: 127-09-3
Molecular Weight: 82.0
Chemical Formula: C₂H₃O₂Na
Solubility: Water
Storage Temp: Room Temperature

Research or further manufacturing use only, not for food or drug use.

• Appearance: White Powder
• Assay: ≥ 99.0 %
• pH (5% Solution): 7.0 - 9.2
• Solubility: Soluble in Water
• Loss on Drying (120 ^oC): ≤ 1.0 %
• Calcium (Ca): ≤ 0.005 %
• Chloride (Cl): ≤ 0.002 %
• Heavy Metals (as Pb): ≤ 0.001 %
• Insoluble Matter: ≤ 0.01 %
• Iron (Fe): ≤ 0.001 %
• Magnesium (Mg): ≤ 0.002 %
• Phosphate: ≤ 0.001 %
• Sulfate (SO₄): ≤ 0.003 %
• Grade: Meet USP/ACS Specifications

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PR1MA™ Imidazole [Glyoxaline] [1,3-Diaza-2-,4-cyclopentadiene]

Common Name: [Glyoxaline] [1,3-Diaza-2,4-cyclopentadiene]
CAS Number: 288-32-4
Molecular Weight: 68.1
Chemical Formula: C₃H₄N₂
Solubility: Water
Storage Temp: Room Temperature

pKa (25°C): 6.9-7.0
pH range: 6.2-7.8

Research or further manufacturing use only, not for food or drug use.

• Appearance: White to off white crystals
• Assay: ≥ 99.0 %
• DNase: Not Detected
• Identification: Pass Test
• Loss on Drying: ≤ 0.5 %
• Melting Point: 88 - 92 ^oC
• OPTICAL @ 260 NM: ≤ 0.2
• PKa @ 25°C: 6.79 - 7.19
• Protease: Not Detected
• RNase: Not Detected

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PR1MA™ Rapid Coomassie Stain

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PR1MA™ Protein Markers

PR1MA™ All Blue, Regular Range Protein Markers

PR1MA™ All Blue, Regular Range Protein Markers are blue protein standards with 10 or 11 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa in Tris-Glycine buffer (9 to 170 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore, and two reference bands (at 25 kDa and 72 kDa, in the presence of Tris- Glycine buffer) are enhanced in intensity.

Loading Volume

• 3 μL or 5 μL per loading for clear visualization during electrophoresis on a 15-well or 10-well mini-gel
• 1.5~2.5 μL per well for general Western blotting transfer

The markers are supplied at a concentration of approximately 0.1~0.5 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C, 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol)

PR1MA™ Tricolor Protein Markers

PR1MA™ Tricolor Protein Markers are three-color protein standards with 10, 12, or 13 pre-stained proteins covering a wide range of molecular weights from 5 to 245 kDa in Tris-Glycine buffer (3.5 kDa to 235 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands, one green and one red band (at 25 kDa and 75 kDa, respectively, in the presence of Tris-Glycine buffer).

Loading Volume

• 3 μL or 5 μL per loading for clear visualization during electrophoresis on a 15-well or 10-well mini-gel.
• 1.5~2.5 μL per well for general Western blotting transfer.

The markers are supplied at a concentration of approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).

Specifications

Item#	Color	Bands	Range
PR5010-BL	Blue	10	9-180 kDA
PR5011-BL	Blue	11	9-180 kDA
PR5010-TC	Tri-Color	10	9-180 kDA
PR5012-TC	Tri-Color	12	9-245 kDA
PR5013-TC	Tri-Color	13	9-245 kDA

 

PR1MA™ Glycerol

CAS Number: 56-81-5
Molecular Weight: 92.1
Chemical Formula: C₃H₈O₃
Solubility: Water
Storage Temp: Room Temperature

Used in sample preparation and gel formation for polyacrylamide gel electrophoresis. Also used in casting gradient gels, as a protein stabilizer and storage buffer component.

Research or further manufacturing use only, not for food or drug use.

• Appearance: Clear and Colorless Liquid
• Chloride (Cl): ≤ 0.001 %
• Chlorinated Compounds: ≤ 0.003 %
• Color, APHA: ≤ 10.0
• Fatty Acids & Esters (USP): ≤ 1.0 (ml 0.5 N NaOH) %
• Glycerine: ≥ 99.7 %
• Heavy Metals: ≤ 5.0 ppm
• Identification C (GC): Pass
• Limit of Diethylene Glycol & RC: Pass
• Purity (USP): 99.0 - 101.0 %
• Residue on Ignition: ≤ 0.01 %
• Specific Gravity: ≥ 1.259
• Water: ≤ 5.0 %
• Date of Manufacture: Report

PR1MA™ Taq

• Ideal for routine PCR applications as well as genotyping, colony PCR, and fast PCR
• Improved template affinity and solubility for higher enzyme activity and greater yields
• Proprietary buffer optimized for a variety of assay conditions
• Conveniently supplied as a 2X Master Mix or in a 2-tube format of polymerase and separate 5X buffer

PR1MA™ Taq provides superior results for routine applications. Modified to improve DNA-binding, this polymerase offers higher solubility and greater template affinity, resulting in consistently superior performance. PR1MA™ Taq Polymerase exhibits a 5' to 3' nuclease activity, but no 3' to 5' (proofreading) activity and works well with a wide range of DNA templates including GC-rich sequences.

The polymerase is supplied with a 5X buffer containing MgCl2 and a proprietary mix of enhancers (dNTP's not included). For convenience, PR1MA Taq is also available in a ready-to-use 2X Master Mix - just add primers and template DNA. Our "Red Dye" Master Mix incorporates a red-loading dye that allows amplified samples to be loaded directly on an agarose gel.  The red color aids in visualization during pipetting, and higher density of the buffer ensures that the samples will drop into the gel wells.

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PR1MA™ SmartGlow™ DNA Safe Stain

• Replaces hazardous Ethidium Bromide (EtBr)
• Better sensitivity than EtBr
• Detect as little as 0.1ng of DNA
• Two types available
• PS Pre Stain
• LD Loading Dye
• Excitation by UV light or blue light
• Compatible with Accuris SmartBlue™ Transilluminator
• Ships at ambient temperature (stored at 4°C)

PR1MA™ SmartStain™ PS (Pre-Stain) can be used as a direct replacement for Ethidium Bromide in agarose and polyacrylamide gel electrophoresis. The stain emits green fluorescence when bound to dsDNA or ssDNA and emits red fluorescence when bound to RNA. PR1MA™ SmartStain™ PS exhibits excitation peaks at 290 nm and 490 nm, allowing it to be used with UV and blue light.

Protocol:

• Prepare 100 mL of agarose or polyacrylamide solution.
• Add 5 uL of PR1MA™ SmartStain™ to the gel solution before pouring gels.
• For enhanced results, add PR1MA™ SmartStain™ PS to the running buffer at a ratio of 5 uL per 100 mL. Adding PR1MA™ SmartStain™ PS to the running buffer will result in increased sensitivity and better detection of small quantities of nucleic acid.
• After electrophoresis is complete, view the gel using a UV or blue light illuminator