Electrophoresis Buffers
QuickSilver - TAE (Tris-acetate-EDTA)

TAE is ideal for separation of large DNA fragments in agarose gels and is used as running buffer and in gel preparation.

TAE is the buffer of choice when recovering DNA from gels and when downstream processing involves enzymatic reactions. Final concentration of a 1X working solution is 40mM Tris, 20mM acetic acid, 1mM EDTA.

QS1000 QuickSilver TAE Buffer, 1X, 100 packs

QuickSilver - TBE (Tris-borate-EDTA)

TBE is used in gel preparation and as a running buffer for electrophoresis of nucleic acids. TBE works best for resolution of smaller fragments (<2kb). Its higher buffering capacity makes it useful for longer runs. The final concentration of a 1X working solution is 89mM Tris, 89mM boric acid, 2mM EDTA.

QS1001 QuickSilver TBE Buffer, 1X, 50 packs

QuickSilver - Fast Running Buffer

QuickSilver Fast Running Buffer is a proprietary blend formulated for agarose gel electrophoresis at high voltage (300-350V). Its strong buffering capacity minimizes heat generation all allows for extremely fast separation of fragments smaller than 5kb. A typical 30-minute run can be performed in just 10 minutes using this buffer.

QS1002 QuickSilver Fast Running Buffer, 1X, 100 packs
 
 

Protein Electrophoresis Buffers

QuickSilver™ TGS (Tris-Glycine-SDS)

Tris-Glycine-SDS is one of the most common running buffers used for SDS-PAGE. A 1X solution contains 25mM Tris, 192mM Glycine and 0.1% SDS.

QS1100 QuickSilver Tris Glycine-SDS Buffer, 1X, 50 packs

QuickSilver™ Tris-MES-SDS

Small to medium molecular weight proteins can be resolved on SDS-PAGE gels using Tris-MES-SDS as a running buffer. Proteins resolve faster in gels using Tris-MES-SDS as a running buffer than those using Tris-MOPS-SDS. A 1X solution has a final concentration of 50mM Tris, 50mM MES, 0.1% SDS and 1mM EDTA.

QS1101 QuickSilver Tris-MES-SDS Buffer, 1X, 50 packs

QuickSilver™ Tris-MOPS-SDS

Tris-MOPS-SDS is ideal for use as a running buffer when resolving medium to large proteins with SDS-PAGE. Gels run with Tris-MOPS-SDS buffer run slower than those using Tris-MES-SDS buffer. The final concentration of a 1X working solution is 50mM Tris, 50mM MOPS, 0.1% SDS and 1mM EDTA.

QS1102 QuickSilver Tris-MOPS-SDS, 1X, 50 packs

Western Blotting, ELISA, Cell Culture, Chromatography

QuickSilver™ PBS and PBST

Because its osmolality and ion concentration closely mimic that of the human body, PBS is commonly used for cell culture applications, as well as HPLC and MS. PBST is a wash buffer and diluent for ELISA. The 1X PBS solution contains 137mN NaCl, 2.7mM KCl and 10mM phosphate. PBST is supplemented with 0.05% Tween-20.

QS1200 QuickSilver PBS Buffer, 1X, 100 packs

QS1201 QuickSilver PBST Buffer, 1X, 100 packs

QuickSilver™ TBS and TBST

TBS and TBST are used to maintain pH within a very narrow range for a variety of applications including western blotting and immunoassays. The final concentration of a 1X working solution is 137mM NaCl, 2.7mM KCl, 50mM Tris. In addition, 1X TBST contains 0.1% Tween-20.

QS1202 QuickSilver TBS buffer, 1X, 100 packs

QS1203 QuickSilver TBST buffer, 1X, 100 packs

Sodium Phosphate Monobasic, Monohydrate

• Appearance (Color): White to Off-White
• Appearance (Form): Crystalline Powder
• Purity: ≥ 99.0%
• pH (5% Aqueous): 8.7 - 9.3
• Identity (FTIR): Conforms to Structure
• Loss on Drying (105°C/4h): 10.0 - 15.0%
• Insoluble Matter: ≤ 0.2%
• Lead: ≤ 4 ppm
• Arsenic: ≤ 3 ppm
• Phosphorous: 20.0 - 24.0%
• Chloride: ≤ 140 ppm
• Fluoride: ≤ 50 ppm
• Sulfate: ≤ 150 ppm
• RNase: None Detected
• DNase: None Detected 

*Custom sizes available upon request.

DOT Information: Non-regulated.   
 

PR1MA™ LB Agar, Low Salt Formula, Granulated [Lennox L Agar]

Common Name: Lennox L Agar
CAS Number: 91079-40-2, 8013-01-2, 7647-14-5, 9002-18-0
Solubility: Water with Heat
Storage Temp: Room Temperature

For propagation and maintenance of E. Coli.

Dissolve 32 grams in 1 Liter of distilled water and adjust the pH to 7.2. Sterilize by autoclaving for 15 minutes.

Ingredients:

• Casein Peptone: 10 g/L
• Sodium Chloride: 5 g/L
• Yeast Extract: 5 g/L
• Agar: 12 g/L

• DCM Appearance: Homogeneous Free Flowing ,Light Beige
• PPM Appearance: Clear, no to light ppt, light yellow
• pH (2.0% Solution @ 25 ^oC): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent
• Sodium Chloride: 5 g/l
• Yeast Extract: 5 g/L
• Agar: 12 g/l
• Casein Peptone: 10 g/L

PR1MA™ LB Broth, Granulated [Lennox LB Broth]

Common Name: Lennox L Broth
Solubility: Water
Storage Temp: Room Temperature

For propagation and maintenance of E. Coli. Low Salt Formula. Molecular Biology Grade.

Dissolve 20 grams in 1 liter distilled water. Then adjust the pH to 7.2. Heat and frequently agitate to completely dissolve. Material is specially granulated to reduce dust and increase solubility rate. Sterilize by autoclaving for 15 minutes.

Ingredients:

• Tryptone:10 g/L
• Sodium Chloride: 5 g/L
• Yeast Extract: 5 g/L

Research or further manufacturing use only, not for food or drug use.

• Appearance: Light beige color, average particle size between 1 to 2mm
• DCM Appearance: Homogeneous, free flowing light beige
• PPM Appearance: Clear, no to light ppt, light yellow
• Appearance of Solution: Prepared medium is yellow to gold and clear to moderately hazy
• pH (2.0% Solution @ 25 ^oC): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent
• Casein Peptone: 10 g/L
• Sodium Chloride: 5 g/L
• Yeast Extract: 5 g/L

PR1MA™  SOB Broth, Capsules

CAS Number: 91079-40-2; 8013-01-2; 7647-14-5; 7487-88-9; 7447-40-7
Solubility: Water
Storage Temp: Room Temperature

SOB Broth is recommended for use in cultivation of recombinant strains of E. Coli. It is a nutritionally rich growth medium for use in the preparation and transformation of competent cells.

Add two capsules to one liter of distilled water. Heat and stir gently to completely dissolve medium. Sterilize by autoclaving at 121°C for 15 minutes.

Final Solution contains:

• Casein Enzymic Hydrolysate: 20 g/L
• Yeast Extract: 5 g/L
• Sodium Chloride: 0.5 g/L
• Magnesium Sulfate: 2.4 g/L
• Potassium Chloride: 0.186 g/L

Research or further manufacturing use only, not for food or drug use.

• Appearance: Capsules
• Appearance of Solution: Prepared medium is light to medium amber and clear
• pH @25 °C: 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

PR1MA™ LB Agar, Low Salt Formula, Powder [Lennox L Agar]

Common Name: Lennox L Agar
CAS Number: 91079-40-2, 8013-01-2, 7647-14-5, 9002-18-0
Solubility: Water with Heat
Storage Temp: Room Temperature

Molecular Biology Grade. For propagation and maintenance of E. Coli. Low Salt Formula.

Dissolve 35 grams in 1 Liter of distilled water. Adjust the pH to 7.2 Sterilize by autoclaving for 15 minutes

Ingredients:

• Casein Peptone: 10 g / L
• Sodium Chloride: 5 g / L
• Yeast Extract: 5 g / L
• Agar: 15 g / L

Research or further manufacturing use only, not for food or drug use.

• Appearance: Powder
• pH: 6.8 - 7.2
• Solubility: Clear Solution

PR1MA™ Agar Noble, Powder

CAS Number: 9002-18-0
Solubility: Water with Heat
Storage Temp: Room Temperature

Ultra-high purity Agar recommended for use in applications where maximum clarity and purity are essential. Thorough bleaching and washing procedures are utilized to remove trace amounts of ash and mineral contaminants.

Concentrations of 3-8 g/L obtains a semi-solid gel.

Research or further manufacturing use only, not for food or drug use.

• Appearance: White to pale yellow crystalline powder
• ASH (Residue Ignition): ≤ 1.6 %
• Gelling Point: 32 - 39 °C
• Melting Point: 80 - 95 ^oC
• Moisture : ≤ 12.0 %
• pH (1.5% solution): 5.5 - 7.2

PR1MA™  Luria Agar Granulated [Miller's LB Agar]

Common Name: Miller's LB Agar
CAS Number: 91079-40-2; 8013-01-2; 7647-14-5; 9002-18-0
Solubility: Water with Heat
Storage Temp: Room Temperature

For propagation and maintenance of E. Coli.

Dissolve 37 grams in 1 Liter distilled water and adjust the pH to 7.2. Sterilize by autoclaving for 15 minutes.

Ingredients:

• Tryptone: 10 g / L
• Sodium Chloride: 10 g / L
• Yeast Extract: 5 g / L
• Agar: 12 g / L

• DCM Appearance: Homogeneous Free Flowing, Light Beige
• PPM Appearance: Clear, none to light ppt, light yellow
• pH (2.5 % Solution @25 ^oC): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

PR1MA™ Peptone, Soy 

CAS Number: 91079-46-8
Solubility: Water
Storage Temp: Room Temperature

From papaic hydrolysis of soybean meal. Low NaCl content.

Research or further manufacturing use only, not for food or drug use.

• Amino Nitrogen: ≥ 2.2 %
• Ash: ≤ 15 %
• Loss on Drying: ≤ 6.0 %
• pH (2% water) @25°C: 6.5 - 7.5
• Total Nitrogen: ≥ 7.0 %
• Coliform: Negative
• E.Coli: Negative
• Molds and Yeasts: ≤ 100 cfu/g
• Salmonella: Negative /25g
• Standard Plate Count : ≤ 5000 cfu/g
• Broth Formulations (Growth Supporting Properties): Satisfactory
• Peptone Agar (Growth Supporting Properties): Satisfactory
• Country of Origin of Raw Material : Report
• Raw Material Species: Plant
• Country of Origin of Enzymes : Report
• Enzyme Species: Porcine
• Country Of Manufacture: Report

PR1MA™ LB Broth Powder [Lennox L Broth]

Common Name: Lennox L Broth
Solubility: Water
Storage Temp: Room Temperature

For propagation and maintenance of E. Coli. Low Salt Formula. Molecular Biology Grade.

Dissolve 20 grams in 1 liter distilled water. Then adjust pH to 7.2. Heat and frequently agitate to completely dissolve. Sterilize by autoclaving for 15 minutes.

Ingredients:

• Tryptone: 10 g/L
• Yeast Extract: 5 g/L
• Sodium Chloride: 5 g/L

Research or further manufacturing use only, not for food or drug use.

• DCM Appearance: Homogeneous free flowing, light beige
• PPM Appearance: Clear, No to light ppt, light yellow
• pH (2% water) @25°C: 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent
• Sodium Chloride: 5 g/L
• Yeast Extract: 5 g/L
• Casein Peptone: 10 g/L

PR1MA™ Terrific Broth, Modified, Granulated

CAS Number: 91079-40-2; 8013-01-2; 7778-77-0; 7758-11-4
Solubility: Water
Storage Temp: Room Temperature

Enriched media for maintenance and propagation of E. Coli and plasmid growth. Produces higher cell densities resulting in greater yields of DNA or recombinant proteins. Glycerol is used as a carbon source with this formulation instead of glucose as it will not ferment to acetic acid.

Ingredients:

• Casein Digest Peptone: 12g/L
• Yeast Extract: 24g/L
• Dipotassium phosphate: 9.5g/L
• Monopotassium phosphate: 2.2g/L

Research or further manufacturing use only, not for food or drug use.

• DCM Appearance: Homogeneous, free flowing beige to yellow-beige
• PPM Appearance: Clear, yellow to amber no precipitate (ppt)
• pH (4.76% solution at 25°C): 6.8 - 7.4
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

PR1MA™ Terrific Broth Modified Capsules

CAS Number: 91079-40-2; 8013-01-2; 7778-77-0; 7758-11-4
Solubility: Water
Storage Temp: Room Temperature

Terrific Broth formulation packaged in easy to use 24 gram capsules. Formulation from powder.

Add 1 capsule per 500 mL of water. Heat and stir until capsules are completely dissolved. Add 2ml of glycerol to mixture. Sterilize by autoclaving for 15 minutes.

Ingredients:

• Casein Digest Peptone: 12 g/L
• Yeast Extract: 24 g/L
• Dipotassium Phosphate: 9.4 g/L
• Potassium Phosphate: 2.2 g/L

Research or further manufacturing use only, not for food or drug use.

• Appearance: Water soluble gelatin capsules containing cream to beige free flowing powder.
• Appearance of Solution: Prepared medium is light to medium amber and clear
• pH (4.76% solution at 25°C): 7.0 - 7.4
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent
• Yeast Extract: 24.0 g/L
• Casein Peptone: 12.0 g/L
• Potassium phosphate dibasic: 9.4 g/L
• Potassium phosphate monobasic: 2.2 g/L
• Direction (Preparation): Add 2 capsules to one liter of purified water until evenly dispersed. Add 4ml of Glycerol to the mixture. Boil for one minute with stirring to completely dissolve. Distribute and autoclave at 121°C for 15 minutes.