Molecular Biology Reagents/Chemicals

MIDSCI™ gives you ultra-pure, cost-competitive molecular biology reagents and lab chemicals for life science research; often with significant cost savings over Sigma Aldrich and other lab reagent providers.

PR1MA™ Chemicals can help with a wide variety of research-based requirements.
 
Use our glycerol for polyacrylamide gel electrophoresis. Eliminate Agrobacterium after inoculation with the carbenicillin we carry. Or detect β-Galactosidase activity with X-GAL which, when combined with the IPTG we stock, can be used to detect lac gene expression in cloning applications.
 
We also carry Luria Broth (Lennox), for the propagation and maintenance of E. coli; DTT, in order to maintain –SH groups in a reduced state; and high gel strength agar for tissue culture and microbiological work.

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PR1MA  Sodium Phosphate Dibasic, Anhydrous, ACS Grade

CAS Number: 7558-79-4
Molecular Weight: 142.0
Chemical Formula: Na2HPO4
Solubility: Water
Storage Temp: Room Temperature

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White crystals
  • Assay:  99.0 %
  • Chloride:  0.002 %
  • Heavy Metals :  10 ppm
  • Insoluble Matter:  0.01 %
  • Iron:  0.002 %
  • Loss on Drying:  0.2 %
  • pH (5% Solution): 8.7 - 9.3
  • Sulfate:  0.005 %
  • Yeast and Mold Count:  100 CFU/g
  • Aerobic Plate Count:  1000 CFU/g

Proudly made in the USA! Click here for more made in America products!
 
 

PR1MA  Paromomycin Sulfate

CAS Number: 1263-89-4
Molecular Weight: 615.6
Chemical Formula: C23H45N5O14  H2SO4
Solubility: Water
Storage Temp: Room Temperature

Aminoglycoside antibiotic with mode of action similar to Kanamycin and Neomycin. Used as a selective agent for the incorporation of the NPT II (APH3') gene in plant tissue. Plant culture tested.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White to Off White Powder
  • Assay: ≥ 675 U/mg
  • Identification: Conforms
  • Loss on Drying:  5.0 %
  • pH 3.0% Solution @25oC: 5.0 -7.5
  • Specific Optical Rotation: +50.0 - +55.0°

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PR1MA Acrylamide/bis-Acrylamide, Premixed Powder 29:1 Ratio

Storage Temp: Room Temperature

Pre-measured powder blend of acrylamide and bis-acrylamide for simple stock solution preparation.

WARNING!
This product can expose you to Acrylamide, a chemical which is known to the State of California to cause cancer, developmental and reproductive harm. For more information go to www.P65Warnings.ca.gov.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White Powder
  • Conductivity of Solution: ≤ 10.0 µmhos
  • Electrophoresis: Pass Test
  • Solubility: Pass Test

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[NTC] [clonNAT] [Streptothricin Sulfate]

PR1MA Nourseothricin Sulfate [NTC] [clonNAT] [Streptothricin Sulfate]

Common Name: [NTC] [clonNAT] [Streptothricin Sulfate]
CAS Number: 96736-11-7
Solubility: Water
Storage Temp: 2-8°C

Dihydrogen sulfate form of the weekly basic antibiotic Nourseothricin consisting of components F, E and D. Effective against gram negative and gram positive bacteria. Also effective against mycobacteria, mycoplasms, protozoa, yeasts, viruses and plants. Commonly used as a selection agent for recombinant yeast strains.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White Powder
  • Carbon (C): Report %
  • Hydrogen (H): Report %
  • Nitrogen (N): Report %
  • Purity (HPLC):  85 %
  • Sulfur (S): Report %

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PR1MA  Bacitracin

CAS Number: 1405-87-4
Molecular Weight: 1422.69
Chemical Formula: C66H103N17O16 S
Solubility: Water, Ethanol, and Methanol
Storage Temp: 2-8°C

Interferes with bacterial cell wall synthesis. Active against gram positive bacteria. Toxic to plant cells.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White to pale buff powder
  • Identification: Pass test
  • pH: 5.5 - 7.5
  • Loss on Drying:  5.0 %
  • Residue on Ignition:  0.5 %
  • Bacitracin A:  40.0 %
  • SUM of bacitracin A, B1, B2 and B3:  70.0 %
  • Limit of early eluting peptides:  20.0 %
  • Limit of Bacitracin F:  6.0 %
  • Microbial Assay (Dried Basis):  65.0 IU / mg
  • Heavy Metals :  30 ppm
  • Total Viable Aerobic Count:  100 CFU/g
  • Particle Size (< 20 µm by volume):  99.0 %
  • Crystallinity : Pass
  • Appearance of Solution: Clear, colorless to slight hazy yellow
  • Source: Bacillus Licheniformis
  • Non Animal Origin: Non Animal Origin
  • Grade: USP
  • Country of Origin : Report

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PR1MA bis-TRIS [bis-(2-hydroxyethyl)aminotris(hydroxymethyl)methane]

Common Name: bis(2-Hydroxyethyl)aminotris (hydroxymethyl)methane
CAS Number: 6976-37-0
Molecular Weight: 209.2
Chemical Formula: C8H19NO5
Solubility: Water
Storage Temp: Room Temperature

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White crystalline powder
  • Assay:  99.0 %
  • Identification (IR): Matches reference
  • Melting Point: Report oC
  • pH, 1.0M Aqueous solution @25ºC: 9.5 - 11.0
  • pKa @ 20°C: 6.0 - 7.0
  • Solubility: Complete and clear
  • UV Absorbance @280nm, 1.0 M in H2O:  0.40
 

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PR1MA Citric Acid Anhydrous

CAS Number: 77-92-9
Molecular Weight: 192.1
Chemical Formula: C6H8O7
Solubility: Water
Storage Temp: Room Temperature

Research or further manufacturing use only, not for food or drug use.

  • Appearance: Fine white crystalline powder
  • Assay (Titration):  99.5 %
  • Carbonizable Substances (H2SO4): Pass Test
  • Clarity and Color: Pass Test
  • Identification: Pass Test
  • Lead :  0.5 mg/kg
  • Limit of Oxalic Acid:  0.036 %
  • Oxalate Test: Pass Test
  • Residual Solvents: Meet requirements
  • Residue on Ignition:  0.05 %
  • Sulfate:  0.015 %
  • Water:  0.5 %

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PR1MA Acrylamide/bis-Acrylamide, Premixed Powder 37.5:1 Ratio

CAS Number: 79-06-1, 110-26-9
Storage Temp: Room Temperature

Pre-measured powder blend of acrylamide and bis-acrylamide for simple stock solution preparation.

WARNING!
This product can expose you to Acrylamide, a chemical which is known to the State of California to cause cancer, developmental and reproductive harm. For more information go to www.P65Warnings.ca.gov.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White powder
  • Conductivity of Solution:  10 µmhos
  • Electrophoresis: Pass Test
  • Solubility: Pass Test

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PR1MA Yeast Nitrogen Base without Amino Acids

Solubility: Water
Storage Temp: Room Temperature

For classifying yeasts based on amino acid and carbohydrates requirements. Contains all essential vitamins and inorganic salts necessary for the cultivation of yeasts. Excludes histidine methionine, tryptophan and carbon source. Also contains <1 mg/L each of vitamins and trace inorganic salts.

Research or further manufacturing use only, not for food or drug use.

  • DCM Appearance: Free flowing powder, Homogeneous, Off white to cream
  • PPM Appearance: Pale to light yellow (10X Solution)
  • pH (0.67% Solution @25 oC): 5.2 - 5.6
  • Microorganism Koleckera Apiculata ATCC 9774 (With Additives): Good to Excellent
  • Microorganism Saccharomyces Cerevisiae ATCC 9080 (With Additives): Good to Excellent
  • Microorganism Saccharomyces Cerevisiae ATCC 9763 (With Additives): Good to Excellent
  • Microorganisms Candida Albicans ATCC 10231 (With Additives): Good to Excellent

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PR1MA  Lysozyme from Chicken Egg White, Salt Free

CAS Number: 12650-88-3
Solubility: Water
Storage Temp: 2-8°C

Purified from chicken egg white, 2x crystallized, dialyzed and supplied as a salt free lyophilized powder.

Dry powder stable for 3 - 5 years when stored dry at 2°C to 8°C.

Stock solution is stable up to 3 days at 4°C and up to 1 year at -20°C.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: 2X Crystallized; dialyzed, lyophilized powder
  • A 280@ mg/ml: Report
  • Protein: Report %
  • U/mg dw (Unit Per mg Dry Weight):  8000
  • Raw Material Species: Chicken Egg Whites
  • Country of Origin: Report

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PR1MA
  Colistin Sulfate

CAS Number: 1264-72-8
Molecular Weight: 2801.27
Chemical Formula: (C52H98N16O13 )2  5(H2SO4)
Solubility: Water
Storage Temp: Short Term: 2-8°C; Long Term: -20°C

Active against gram negative bacteria. Potency approximately 20,000 units per milligram.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White or almost white powder
  • Arsenic (As):  2 ppm
  • Heavy Metals : ≤ 20 ppm
  • Identification: Conforms
  • Loss on Drying:  3.5 %
  • pH (1% in H2O): 4.0 - 6.0
  • Potency (Dried basis):  19000 µg/mg
  • Solubility (50mg/ml in H2O): Clear and light yellow solution
  • Specific Rotation: -73 - -63 °
  • Sulfate: 16.0 - 18.0 %
  • Sulfated Ash:  1.0 %

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PR1MA  Polymixin B Sulfate

CAS Number: 1405-20-5
Molecular Weight: 1385.61
Chemical Formula: C55H96N16O13  2H2SO4
Solubility: Water
Storage Temp: 2-8°C

Mixture of sulfates of polypeptides from certain strains of Bacillus polymixa. Active against gram negative bacteria.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White to Light Yellow Powder
  • Identification: Pass Test
  • pH (0.5% Solution): 5.0 - 7.5
  • Loss on Drying:  7.0 %
  • Single Impurity:  3.0 %
  • Total impurities:  17.0 %
  • Bacteria Colonies:  10/g
  • Ecoli Colonies: Should not be found
  • Fungi and Yeast colonies:  10/g
  • Assay, HPLC (on dried basis) (Sum of polymxin B1,B2, B3 and B1-1):  80.0 %
  • Assay, HPLC (on dried basis) (Polymyxin B3):  6.0 %
  • Assay, HPLC (on dried basis) (Polymyxin B1-1):  15.0 %
  • Assay (Dried Basis):  6000 U/mg
  • Assay (Wet Basis): Report U/mg
  • Non Animal Origin: Non Animal Origin
  • Grade: USP
  • Country of Origin: Report

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