3D Cell Culture

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroGel® Angiogenesis Assay Kit
 
ready-to-use, hydrogel system for 2D gel coating and 3D culture of angiogenesis tube formation, invasion, and animal injection.

• Hydrogel - Hydrogel Only: VitroGel® AAK (2 mL)
• Type 1 - Full Kit (Hydrogel+Supplements): VitroGel AAK (2 mL), AAK Supplement 1 (500 µL), AAK Supplement 2 (500 µL)
• Type 2 - Full Kit (Hydrogel+Supplements): VitroGel AAK (2 mL), AAK Supplement 1 (2 x 500 µL)
• Type 3 - Full Kit (Hydrogel+Supplements): VitroGel AAK (2 mL), AAK Supplement 2 (2 x 500 µL)

• Xeno-free hydrogel system supporting angiogenesis process
• Multiple applications in one kit:  Tube formation, invasion, and animal injection
• Control the hydrogel properties:  Add your own growth factors and compare with positive control
• Easy, efficient, and enzyme-free cell harvesting in 20 minutes

VitroGel® Angiogenesis Assay Kit is a revolutionary tool for researchers to study the effect of both hydrogel properties and culture medium on angiogenesis process. The kit can be used to study the angiogenesis tube formation and invasion on both 2D hydrogel coating method and 3D cell culture method. The VitroGel® system is also good for animal injection for in vivo study.

Angiogenesis is a highly regulated process that involves the growth of new blood vessels from the existing vasculature. This process plays an important role in both normal developmental processes and numerous pathologies, including wound healing, tumor growth, and metastasis to inflammation and ocular disease.

Traditional angiogenesis assay highly relies on natural extracellular matrix (natural ECM), which has non-adjustable hydrogel compositions and properties.  Therefore, our understanding of the angiogenesis process is limited by studying the molecular cues such as growth factors and inhibitors in culture medium only. There is a lack of knowledge on how the properties of hydrogel affect the angiogenesis process.

There are two versions of VitroGel® Angiogenesis Assay Kits:

• VitroGel® Angiogenesis Assay Kit (Ready-To-Use):  Ready-to-use with a fixed hydrogel mechanical strength to support the angiogenesis assay with adjustable supplements
• VitroGel® Angiogenesis Assay HC Kit (Cat No. TWG011-K):  Assay kit with a tunable high concentration hydrogel to allow full control of the hydrogel’s mechanical strength with adjustable supplements.

Depending on the kit type, the ready-to-use VitroGel® Angiogenesis Assay Kits can contain the following components:

• VitroGel® AAK, a xeno-free ready-to-use hydrogel.
• AAK Supplement 1, a hydrogel supplement without vascular endothelial growth factors (VEGFs) for cell attachment and growth.
• AAK Supplement 2, a hydrogel supplement with VEGFs to promote tube formation and as a positive control.

The VitroGel® AAK hydrogel is room temperature stable and can be directly mixed with each supplement at 2:1 (v/v) ratio for hydrogel formation.  Researchers can adjust the hydrogel’s molecular cues by adding the growth factors/inhibitors directly to the supplements before mixing with the VitroGel® AAK hydrogel.  Cells cultured in this system can be harvested further easily with the VitroGel® Cell Recovery Solution.

Contents and Specifications

TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.   

TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.

To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored. 

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroGel® ORGANOID
 
ready-to-use, xeno-free (animal origin-free) hydrogel system for organoid culture

• Xeno-free hydrogel for organoid formation
• Supports a wide range of organoids from patient-derived samples, stem cells, tissues, co-culture and PDX resources
• Scalable protocol for lab automation
• Easy 20-minute cell harvesting (enzyme-free)
• VitroGel® ORGANOID (1-4) are xeno-free (animal origin-free) hydrogels that support the growth of patient-derived organoids or organoids developed from pluripotent stem cells (PSCs), co-culture, and PDX model.

VitroGel® ORGANOID hydrogels are ready to use at room temperature and have a neutral pH, transparent, permeable, and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. VitroGel® ORGANOID hydrogels are good for both 3D cell culture and 2D hydrogel coating applications.

The VitroGel® ORGANOID hydrogels can work in conjunction with VitroGel® STEM (a xeno-free hydrogel for 3D static suspension cultures and scale-up of human pluripotent stem cells) by transferring stem cell spheroids cultured in VitroGel® STEM to VitroGel® ORGANOID hydrogels for organoid differentiation. See Xeno-free 3D Organoid Workflow B.  Key growth factors and molecules can be directly mixed with the hydrogel matrix or by adding on the top of the hydrogel. Organoids cultured in this system can be easily harvested out with our VitroGel® Cell Recovery Solution.  VitroGel® ORGANOID hydrogels provide a well-defined 3D microenvironment for the future of personalized medicine.

Which organoid hydrogel to choose

We still strongly suggest starting with the VitroGel® ORGANOID Discovery Kit (Item # VHM04-K) to make a quick screening to find the hydrogel version that is optimal for the organoid conditions.

The kit includes all four types of organoid hydrogels:

VitroGel® ORGANOID-1, VitroGel® ORGANOID-2, VitroGel® ORGANOID-3, VitroGel® ORGANOID-4)

Each of the four types has various bio-functional ligands, mechanical strengths, and degradability to fulfill the needs of different organoid culture conditions.

Because there are a wide range of organoid cell resources (from stem cells, patient-derived tissue, co-culture, to PDX), it is hard to tell which organoid hydrogel would best perform the researcher’s experiment. Therefore, the Discovery Kit helps researchers to screen the four different formulations to determine the best organoid hydrogel version for the organoid conditions.

From our findings, versions 1, 2, or 3 are suitable for gastric organoids. Versions 1 or 3 are suitable for lung organoids. Versions 2 or 3 are suitable for brain organoids, and versions 3 or 4 are suitable for cancer organoids.

Specifications

TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.   

TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.

To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

AAK Supplement
 
Hydrogel supplements for use with the VitroGel® Angiogenesis Assay Kits

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroGel® MSC
 
Mesenchymal Stem Cell 3D Culture and Scale-up

VitroGel® MSC is a xeno-free (animal origin-free) hydrogel system developed to support three-dimensional (3D) cultures of mesenchymal stem cells (MSCs).  This hydrogel system can be used to make hydrogel cell beads for MSC scale-up.  Microcarriers are not required for MSC scale-up.

VitroGel® MSC is ready-to-use with an optimized formulation that fully supports the rapid expansion of MSCs. Cells directly thawed from liquid nitrogen or passaged from 2D culture vessels can be immediately mixed with the hydrogel solution for 3D culture or hydrogel-cell bead generation. This hydrogel system is compatible with most MSC culture media and tissue culture vessels. By using the VitroGel® Cell Recovery Solution, cell harvesting after 3D culture is simple and efficient.

3D cell culture process in 20 min – “Just add cells”

VitroGel® MSC is ready-to-use. Just mix with your cells.

Specifications

TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.   

TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.

To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroGel® MMP High Concentration
 
Matrix metalloproteinases (MMP) sensitive biodegradable hydrogel – tunable, xeno-free hydrogel, high concentration (3 mL kit)

• Dilution Solution TYPE 1 contains sucrose for best osmolarity
• Dilution Solution TYPE 2 is a sucrose-free formulation for cells sensitive to the sugar level in the medium

VitroGel® MMP High Concentration is a tunable, xeno-free (animal origin-free) hydrogel system modified with matrix metalloproteinase-sensitive peptides. The enzyme-sensitive hydrogel can be degraded by several MMPs (MMP1, MMP2, MMP3, MMP9, and MMP13) and support different biological activities such as cell proliferation, migration (adhesion/dispersion), differentiation, angiogenesis, apoptosis, etc.  VitroGel® MMP High Concentration comes with VitroGel® Dilution Solution to adjust the final hydrogel strength from 10 to 4000 Pa.

VitroGel® High Concentration hydrogels are our xeno-free, tunable hydrogels for researchers wanting full control to manipulate the biophysical and biological properties of the cell culture environment. The tunability of the hydrogel gives the ability to create an optimized environment for cell growth. The hydrogel system has a neutral pH, transparent, permeable, and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. No cross-linking agent is required. Cells cultured in this system can be easily harvested with our VitroGel® Cell Recovery Solution. The hydrogel can also be tuned to be injectable for in vivo studies.

From 3D cell culture, 2D cell coating to animal injection, VitroGel® makes it possible to bridge the in vitro and in vivo studies with the same platform system.

Specifications 

 TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.   

TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.

To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.

TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.   

TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.

To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroGel® STEM
 
ready-to-use, xeno-free (animal origin-free) hydrogel system for hPSCs 3D static suspension culture and scale-up

• Establish stem cell spheroids directly from liquid nitrogen (5 min protocol)
• High-quality 3D stem cells for differentiation and organoid formation
• Easy scale-up (from laboratory scale to industrial scale)
• Efficient cell harvesting or subculturing

VitroGel® STEM is a xeno-free (animal origin-free) hydrogel system developed to improve the performance of three-dimensional (3D) static suspension cultures and scale-up of human pluripotent stem cells (hPSCs) to create a high-throughput system to model various tissue and disease states.

This hydrogel system is ready to use with an optimized formulation that fully supports the rapid expansion of high-quality 3D stem cell spheroids with pluripotent properties. hPSCs directly thawed from liquid nitrogen or passaged from 2D matrix coated culture vessels can be immediately mixed with the hydrogel solution for static suspension cultures. Moreover, the optimization protocol is ideal for time-sensitive experiments, as it does not require excessive medium exchanges, which can ultimately save on time and materials. This hydrogel system is compatible with most hPSC culture media and tissue culture vessels. Due to the unique static suspension culture procedure, the requirement for microcarriers for large-scale bioreactors is eliminated, making cell harvesting simple and effective. The 3D stem cell spheroids that are developed using this system can be used for further sub-cultures, patterned differentiations, organoid development, or re-establishing 2D culture morphologies.

“Just add cells” 5 min protocol. No matrix coating required.

VitroGel® STEM is ready-to-use. Just mix with your hPSCs. There is no laborious matrix coating required to maintain and expand your stem cells.

Specifications

TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.   

TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.

To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroGel® Cell Recovery Solution (100 mL)
 
Enzyme-free cell harvesting solution to recover cells from the hydrogel in 20 min.

VitroGel® Cell Recovery Solution is an enzyme-free, ready-to-use solution to harvest 2D, 3D, and organoids cultured cells efficiently and safely. The solution can recover cells from VitroGel® hydrogels in 20 minutes.

VitroGel® Cell Recovery Solution is room temperature stable, has a neutral pH, and works at 37°C operating temperature. The solution can maintain high cell viability during the recovery process. Harvested cells can be sub-culture in both 2D and 3D cultures.

The VitroGel® Cell Recovery Solution can be used before or after the fixation and stained preparation of hydrogel specimens to ensure high-quality downstream data analysis.

Specifications 

TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.    

TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.
 

To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroGel® IKVAV High Concentration
 
Laminin-derived functional peptide (IKVAV) modified – tunable, xeno-free hydrogel, high concentration (3 mL kit)

• Dilution Solution TYPE 1 contains sucrose for best osmolarity 
• Dilution Solution TYPE 2 is a sucrose-free formulation for cells sensitive to the sugar level in the medium

VitroGel® IKVAV High Concentration is a tunable, xeno-free (animal origin-free) hydrogel system modified with laminin-derived functional peptide (IKVAV). IKVAV is the bioactive sequence located on the C-terminal end of the long arm of the α-1 chain of laminin, which is actively involved in different biological activities such as neuronal progenitor cell differentiation, promoting cell adhesion, neurite outgrowth, angiogenesis, and tumor growth.  VitroGel® IKVAV High Concentration comes with VitroGel® Dilution Solution to adjust the final hydrogel strength from 10 to 4000 Pa.

VitroGel® High Concentration hydrogels are our xeno-free, tunable hydrogels for researchers wanting full control to manipulate the biophysical and biological properties of the cell culture environment. The tunability of the hydrogel gives the ability to create an optimized environment for cell growth. The hydrogel system has a neutral pH, transparent, permeable and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. No cross-linking agent is required. Cells cultured in this system can be easily harvested with our VitroGel® Cell Recovery Solution. The hydrogel can also be tuned to be injectable for in vivo studies.

From 3D cell culture, 2D cell coating to animal injection, VitroGel® makes it possible to bridge the in vitro and in vivo studies with the same platform system.

Specifications

TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.

To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroGel® YIGSR High Concentration
 
Laminin-derived functional peptide (YIGSR) modified – tunable, xeno-free hydrogel, high concentration (3 mL kit)

• Dilution Solution TYPE 1 contains sucrose for best osmolarity
• Dilution Solution TYPE 2 is a sucrose-free formulation for cells sensitive to the sugar level in the medium

VitroGel® YIGSR High Concentration is a tunable, xeno-free (animal origin-free) hydrogel system modified with laminin-derived functional peptide (YIGSR). YIGSR sequence resides on the laminin β1 chain, which involves endothelial cell adhesion, cell proliferation and motility/migration. VitroGel® YIGSR High Concentration comes with VitroGel® Dilution Solution to adjust the final hydrogel strength from 10 to 4000 Pa.

VitroGel® High Concentration hydrogels are our xeno-free, tunable hydrogels for researchers wanting full control to manipulate the biophysical and biological properties of the cell culture environment. The tunability of the hydrogel gives the ability to create an optimized environment for cell growth. The hydrogel system has a neutral pH, transparent, permeable and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. No cross-linking agent is required. Cells cultured in this system can be easily harvested with our VitroGel® Cell Recovery Solution. The hydrogel can also be tuned to be injectable for in vivo studies.

From 3D cell culture, 2D cell coating to animal injection, VitroGel® makes it possible to bridge the in vitro and in vivo studies with the same platform system.

Specifications 

TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.   

TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans. To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored. 

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroGel® COL High Concentration
 
collagen-mimetic functional hydrogel – tunable, xeno-free (3 mL)

• Dilution Solution TYPE 1 contains sucrose for best osmolarity
• Dilution Solution TYPE 2 is a sucrose-free formulation for cells sensitive to the sugar level in the medium

VitroGel® COL High Concentration is a tunable, xeno-free hydrogel system that mimics the functions of native collagen. The hydrogel can promote osteoblastic differentiation in vitro and enhancing osteoblastic activity in vivo, which shows great potential for tissue engineering and regeneration medicine application. VitroGel® COL High Concentration comes with VitroGel® Dilution Solution to adjust the final hydrogel strength from 10 to 4000 Pa.

VitroGel® High Concentration hydrogels are our xeno-free, tunable hydrogels for researchers wanting full control to manipulate the biophysical and biological properties of the cell culture environment. The tunability of the hydrogel gives the ability to create an optimized environment for cell growth. The hydrogel system has a neutral pH, transparent, permeable, and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. No cross-linking agent is required. Cells cultured in this system can be easily harvested with our VitroGel® Cell Recovery Solution. The hydrogel can also be tuned to be injectable for in vivo studies.

From 3D cell culture, 2D cell coating to animal injection, VitroGel® makes it possible to bridge the in vitro and in vivo studies with the same platform system.

Specifications 

TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.    

TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.
 

To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.