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For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.
VitroINK® 10:1 Mixing Component Pack
Single-use components for 10:1 mixing ratio for VitroINK®
Mixing Kit. DISPENSER NOT INCLUDED
The VitroINK® 10:1 Mixing Component Kit includes a 1 mL syringe, connector and mixing head to replace the one-time-use components in the full VitroINK® Mixing Kit – Complete Pack (Item # IMK00-1) for 10:1 VitroINK® and cells mixing.
This package is sterilized and disposable for one-time use.
Kit Contents and Specifications
- Disposable mixing kit components for VitroINK® kit:
- (1) 1 mL syringe
- (1) connector and tubing
- (1) mixing head
- Sterile
- Use for 10:1 mixing ratio
- Ships room temperature. Store at room temperature.
TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.
TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.
To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.
For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.
VitroINK® 3:1 Mixing Component Pack
Kit Contents and Specifications
- Disposable mixing kit components for VitroINK kit:
- (1) 3 mL syringe
- (1) connector and tubing
- (1) mixing head
- Sterile
- Use for 3:1 mixing ratio
- Ships room temperature. Store at room temperature.
TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.
TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.
To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.
For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.
VitroINK® Mixing Kit – Complete Pack
Mix VitroINK® bioink with cells at 3:1 and 10:1 ratios. Full
mixing kit with Dispenser and single-use components.
Mixing cells with the bioink is a critical step for 3D bioprinting. Because of the differences in viscosity of the cell suspension and bioink, the traditional manual mixing methods create a lot of air bubbles and non-uniform mixture which leads to the unstable printed structure, difficulty for cell observation and affect cell viability.
The VitroINK® Mixing Kit was designed to provide a robust mixing of the bioink and cells. Cells can be prepared with VitroINK® for a 3:1 mixing ratio using the 3 mL syringe or a 10:1 mixing ratio using the 1 mL syringe. To prepare the bioprinter cartridge, the VitroINK® and cell suspension are placed in the mixer and is dispensed through the connector and mixing head. Wait 10-20 minutes for the mixture to stabilized and the cartridge is ready for printing. There is no UV, no temperature/pH curing, or chemical cross-linking for the VitroINK® system. Adding cell culture medium to cover the printed structure can further stabilize and support cell growth. The bioprinted cells are ready for incubation.
The syringe, connector and mixing head are disposable for
one-time use. Order the sterilized replacement mixing kit components to ensure
the robust results for every single mixing.
Kit Contents and Specifications
- Disposable mixing kit components for VitroINK bioink:
- (1) Dispenser
- (1) 1 mL syringe
- (1) 3 mL syringe
- (1) connector and tubing
- (1) mixing head
- Sterile
- Use for 3:1 or 10:1 mixing ratio
- Ships room temperature. Store at room temperature.
TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.
TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.
To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.
VitroINK® Bioinks
Ready-to-use bioinks for 3D bioprinting
VitroINK® are ready-to-use, xeno-free (animal origin-free) bioink system either unmodified or modified with either RGD, Collagen-memetic, IKVAV, or YIGSR peptides or with Matrix Metalloproteinases (MMP) for biodegradable bioink.
VitroINK® is a family of the ready-to-use, xeno-free tunable bioink system that requires no UV, no temperature/pH curing, or chemical cross-linking. The system is ready-to-use at room temperature, neutral in pH, transparent, and has excellent visibility after printing and cell culture. Due to the unique shear-thinning and rapid recovery mechanical property, VitroINK® can maintain the printed structure without UV or other special curing methods. Adding cell culture medium after printing can further stabilize the printed structure and support cell growth. Cells can be pre-mix with VitroINK® using our VitroINK® Mixing Kit for mixing ratios at 3:1 or 10:1. Different versions of VitroINK® may incorporate multiple biological functional ligands to promote cell attachment, cell-matrix interactions, cell proliferation, motility/migration and differentiation for many different applications.
Specifications
- Xeno-free tunable modified bioink.
- Ready-to-use at room temperature
- No UV, temperature/pH curing, or chemical cross-linking required
- Neutral pH
- Transparent. Excellent visibility after printing and cell culture
- Pre-mix with cells using the included VitroINK® Mixing Kit
- Ships room temperature. Store at 2-8°C
- Size: 3 mL
TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.
TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.
To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.
For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.
VitroINK® – Bioink Starter Kits
VitroINK® (3 mL) + Full Mixing Kit with Dispenser
VitroINK® Bioink Starter Kit contains both bioink and the
complete mixing kit to prepare the cell bioink mixture for 3D bioprinting. The
kit includes choice of bioink + the mixing kit:
- VitroINK 3D (3 mL) + VitroINK® Mixing Kit with Dispenser
- VitroINK RGD (3 mL) + VitroINK® Mixing Kit with Dispenser
- VitroINK COL (3 mL) + VitroINK® Mixing Kit with Dispenser
- VitroINK MMP (3 mL) + VitroINK® Mixing Kit with Dispenser
- VitroINK IKVAV (3 mL) + VitroINK® Mixing Kit with Dispenser
- VitroINK YIGSR (3 mL) + VitroINK® Mixing Kit with Dispenser
The VitroINK® Mixing Kit includes a dispenser, 1 mL syringe, 3 mL syringe, connectors and mixing head to get you started.
VitroINK® is a ready-to-use tunable bioink modified with functional ligands, and cell adhesive peptide, promoting cell attachment and cell-matrix interactions. This RGD modified bioink can enhance cell adhesion, proliferation, motility/migration, and differentiation in different applications.
VitroINK® Mixing Kit was designed to provide a robust mixing of bioink and cells. Cells can be prepared with VitroINK® for a 3:1 mixing ratio using the 3 mL syringe or a 10:1 mixing ratio using the 1 mL syringe. Put the VitroINK® and cell suspension in the mixer and gently press the mixture through the connector and mixing head to your bioprinter cartridge. Wait 10-20 minutes for the mixture to stabilize before printing. There is no UV, no temperature/pH curing, or chemical cross-linking for the VitroINK® system. Simply adding cell culture medium to cover the printed structure can further stabilize and support cell growth. The bioprinted cells are ready for incubation.
Contents and Specifications
VitroINK®
- Xeno-free tunable modified bioink.
- Ready-to-use at room temperature
- No UV, temperature/pH curing, or chemical cross-linking required
- Neutral pH
- Transparent. Excellent visibility after printing and cell culture
- Pre-mix with cells using the included VitroINK® Mixing Kit
- Ships room temperature. Store at 2-8°C
- Size: 3 mL
VitroINK® Mixing Kit
- Complete mixing kit. Includes:
- (1) 1 mL syringe
- (1) 3 mL syringe
- (1) connector and tubing
- (1) mixing head
- Sterile
- Use for 3:1 or 10:1 mixing ratio
- Size: 3 mL
TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.
TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.
To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.
For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.
VitroGel® Dilution Solution (50 mL)
For hydrogel concentration adjustment with the high
concentration hydrogels.
- Type 1 - Contains sucrose to maintain the best osmolarity.
- Type 2 - Sucrose-free formulation for cells sensitive to the sugar level in the medium.
VitroGel® Dilution Solution is a ready-to-use solution to be used with the VitroGel® High Concentration hydrogels to adjust the hydrogel concentration for different hydrogel strength.
The solution is room temperature stable with a neutral pH,
which maintains a good physiological condition for hydrogel formation and can
accelerate the hydrogel formation in combination with the cell medium. The
Dilution Solution can be used to prepare a thin gel coating plate or injectable
hydrogel with the high concentrations hydrogels.
Specifications
- Ready-to-use to adjust the concentration of VitroGel® High Concentration hydrogels
- Neutral pH
- Room temperature stable
- Transparent
- Compatible with VitroGel® system for 2D & 3D cell culture and hydrogel injection
- Ships and store at room temperature
- Size: 50 mL
TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.
For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.
VitroGel® Angiogenesis Assay HC Kit
Tunable hydrogel system for 2D gel coating and 3D culture of
angiogenesis tube formation, invasion, and animal injection.
- Hydrogel Kit Only: VitroGel® AAK-HC hydrogel ( 1 mL), AAK Dilution Solution (10 mL)
- Type 1 Full Kit (Hydrogel+Supplements): VitroGel® AAK-HC (1 mL), AAK Dilution Solution (10 mL), AAK Supplement 1 (3 x 500 µL), AAK Supplement 2 (3 x 500 µL)
- Type 2 Full Kit (Hydrogel+Supplements): VitroGel® AAK-HC (1 mL), AAK Dilution Solution (10 mL), AAK Supplement 1 (6 x 500 µL)
- Type 3 Full Kit (Hydrogel+Supplements): VitroGel® AAK-HC (1 mL), AAK Dilution Solution (10 mL), AAK Supplement 2 (6 x 500 µL)
VitroGel® Angiogenesis Assay HC Kit
- Xeno-free functional hydrogel system supporting angiogenesis process
- Control and study the effect of the hydrogel mechanical properties on the angiogenesis process
- Multiple applications in one kit: Tube formation, invasion, and animal injection
- Control the hydrogel properties: Add your own growth factors and compare with positive control
- Harvest cells within 20 minutes at 37 °C without enzyme solution
VitroGel® Angiogenesis Assay Kit is a revolutionary tool for researchers to study the effect of both hydrogel properties and culture medium on angiogenesis process. The kit can be used to study the angiogenesis tube formation and invasion on both 2D hydrogel coating and 3D cell culture methods. The VitroGel® system is also good for animal injection for in vivo studies.
Angiogenesis is a highly regulated process that involves the growth of new blood vessels from the existing vasculature. This process plays an important role in both normal developmental processes and numerous pathologies, including wound healing, tumor growth, and metastasis to inflammation and ocular disease.
Traditional angiogenesis assay highly relies on natural extracellular matrix (natural ECM), which has non-adjustable hydrogel compositions and properties. Therefore, our understanding of the angiogenesis process is limited by studying the molecular cues such as growth factors and inhibitors in culture medium only. There is a lack of knowledge on how the properties of hydrogel affect the angiogenesis process.
There are two versions of VitroGel® Angiogenesis Assay Kits:
- VitroGel® Angiogenesis Assay Kit (Cat No. VHM06-K): Ready-to-use with a fixed hydrogel mechanical strength to support the angiogenesis assay with adjustable supplements.
- VitroGel® Angiogenesis Assay HC Kit (Cat No. TWG011-K): Assay kit with a tunable high concentration hydrogel to allow full control of the hydrogel’s mechanical strength with adjustable supplements.
Depending on the kit type, the tunable VitroGel Angiogenesis
Assay HC Kit can contain the following components:
- VitroGel® AAK-HC, a tunable, xeno-free high concentration hydrogel.
- AAK Dilution Solution, for adjusting the hydrogel concentration
- AAK Supplement 1, a hydrogel supplement without vascular endothelial growth factors (VEGFs) for promoting cell attachment and growth.
- AAK Supplement 2, a hydrogel supplement with VEGFs to promote tube formation and as a positive control.
- All the components can be purchased separately.
Besides molecular cues, the VitroGel® Angiogenesis Assay HC
Kit allows researchers to explore the effects of hydrogel mechanical properties
on angiogenesis. The high concentration
VitroGel® AAK-HC hydrogel is room temperature stable and can be adjusted by simply
mixing the hydrogel solution and dilution solution at different rations
(recommend 1:1 to 1:5 v/v) to achieve different mechanical strengths. The
diluted hydrogel solution can be directly mixed with supplement at 2:1 (v/v)
ratio for hydrogel formation. Researchers can adjust the molecular cues of the
hydrogel by adding the growth actors/inhibitors directly to the supplement
before mixing with VitroGel® AAK-HC.
Cells cultured in this system can be further harvested easily with the
VitroGel® Cell Recovery Solution.
|
TYPE
1 Kit Contents |
VitroGel®
AAK-HC (1 mL) |
|
AAK
Dilution Solution (10 mL) |
|
|
AAK
Supplement 1 (3 x 500 µL) |
|
|
AAK
Supplement 2 (3 x 500 µL) |
|
|
TYPE
2 Kit Contents |
VitroGel®
AAK-HC (1 mL) |
|
AAK Dilution
Solution (10 mL) |
|
|
AAK
Supplement 1 (6 x 500 µL) |
|
|
TYPE
3 Kit Contents |
VitroGel®
AAK-HC (1 mL) |
|
AAK
Dilution Solution (10 mL) |
|
|
AAK
Supplement 2 (6 x 500 µL) |
|
|
Formulation |
Tunable,
xeno-free functional hydrogel |
|
AAK Supplement
1: Without VEGFs |
|
|
AAK
Supplement 2: With VEGFs |
|
|
Use |
Angiogenesis
Assay, tube formation, invasion, animal injection |
|
Biocompatibility |
Biocompatible,
safe for animal studies |
|
Injection |
Injectable
hydrogel for in vivo studies and lab automation |
|
Cell
Harvesting |
Easy
cell recovery using VitroGel® Cell Recovery Solution |
|
pH |
Neutral |
|
Shipping |
Supplements
require dry ice shipment. |
|
Storage |
VitroGel®
AAK-HC hydrogel: store at 2-8°C |
|
AAK
Supplement 1 and AAK Supplement 2: store at -20°C |
|
|
Number
of Uses |
30-180
tests per kit |
TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.
TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.
To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.
For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.
Cyto3D™ Live-Dead Assay Kit
Live dead cell viability analysis for 3D and 2D cell
culture.
The Cyto3D™ Live-Dead Assay Kit is used to determine the live/dead nucleated cells by using a fast one-step staining procedure for analysis on a dual-fluorescence system. This kit is recommended for viability analysis of cells cultured in 3D, 2D coating and on monolayer.
- Ready-to-use
- Fast
- Sensitive
- Excellent for 3D cell cultures
- Cost-effective
Acridine orange (AO) and propidium iodide (PI), both nuclear staining (nucleic acid binding) dyes, are used in this kit. AO is permeable to both live and dead cells and stains all nucleated cells to generate green fluorescence. PI only penetrates the membranes of nucleated cells with compromised membranes and stains the dead cells to generate red fluorescence. Due to the quenching, when cells are stained with both AO and PI, all live nucleated cells fluoresce green and all dead nucleated cells fluoresce red (the PI reduces the fluorescence intensity of the AO by fluorescence resonance energy transfer (FRET)). Non-nucleated materials such as red blood cells, platelets and debris do not fluorescence and are ignored by fluorescence microscopes.
Dual-Fluorescence Viability, using AO and PI, is the
recommended viability analysis method for cell lines, primary cells, and stem
cells.
Specifications
|
Formulation |
Premixed
acridine orange (AO) and propidium iodide (PI), nuclear staining dyes |
|
Use |
Live
dead cell viability analysis for 3D and 2D cell culture |
|
Detection
Method |
Fluorescent |
|
Excitation/Emission: |
AO
(494/517nm), |
|
PI
(535/617nm) |
|
|
Standard
filters |
AO
(GFP), PI (Texas Red) |
|
For
use with (Equipment): |
Fluorescence
microscope, flow cytometer, microplate reader, fluorescence cell counter. |
|
Storage |
2 to
8°C (Protect from light) |
|
Shipping
Conditions: |
Ships
at ambient temperature |
|
Sizes |
1 mL |
|
Number
of reactions |
500
(at 2 µL per 100 µL) |
TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.
TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.
To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.
For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.
VitroGel® RGD High Concentration
RGD modified – tunable, xeno-free hydrogel – high concentration (3 mL kit)
- Dilution Solution TYPE 1 contains sucrose for best osmolarity
- Dilution Solution TYPE 2 is a sucrose-free formulation for cells sensitive to the sugar level in the medium
VitroGel® RGD High Concentration is a tunable, xeno-free (animal origin-free) hydrogel system modified with cell adhesive peptide RGD to promote cell attachment and cell-matrix interactions during the 3D cell culture. VitroGel® RGD High Concentration comes with VitroGel Dilution Solution to adjust the final hydrogel strength from 10 to 4000 Pa.
VitroGel® High Concentration hydrogels are our xeno-free, tunable hydrogels for researchers wanting full control to manipulate the biophysical and biological properties of the cell culture environment. The tunability of the hydrogel gives the ability to create an optimized environment for cell growth. The hydrogel system has a neutral pH, transparent, permeable and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. No cross-linking agent is required. Cells cultured in this system can be easily harvested with our VitroGel® Cell Recovery Solution. The hydrogel can also be tuned to be injectable for in vivo studies.
From 3D cell culture, 2D cell coating to animal injection,
VitroGel makes it possible to bridge the in vitro and in vivo studies with the
same platform system.
|
Contents |
VitroGel® RGD
High Concentration, 3 mL |
|
VitroGel® Dilution
Solution, 50 mL |
|
|
Hydrogel
Formulation |
Xeno-free
tunable hydrogel modified with RGD peptide. |
|
HIGH
CONCENTRATION |
|
|
Use |
Good
for adhesion cells or cells requiring stronger cell-matrix interactions. |
|
Mix
& Match |
Can be blended with other versions of VitroGel® to create a multi-functional hydrogel |
|
Operation |
Room
temperature |
|
Hydrogel
Strength |
10 to
4,000 Pa of G’ depending on dilution ratio. |
|
Dilute with VitroGel® Dilution Solution (TYPE 1 or TYPE 2) for different concentrations. |
|
|
pH |
Neutral |
|
Color |
Transparent |
|
Cell
Harvesting |
20 min cell recovery using VitroGel® Cell Recovery Solution |
|
Injectable |
Injectable
hydrogel |
|
Storage |
Store
at 2-8°C. Ships at ambient temperature |
|
Number
of Uses |
Dilution
ratio: |
|
1:2 =
225 uses at 50 µL per well |
|
|
1:3 =
300 uses at 50 µL per well |
|
|
1:5 =
450 uses at 50 µL per well |
TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.
TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.
To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.
For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.
VitroGel® Angiogenesis Assay Kit
ready-to-use, hydrogel system for 2D gel coating and 3D
culture of angiogenesis tube formation, invasion, and animal injection.
- Hydrogel - Hydrogel Only: VitroGel® AAK (2 mL)
- Type 1 - Full Kit (Hydrogel+Supplements): VitroGel AAK (2 mL), AAK Supplement 1 (500 µL), AAK Supplement 2 (500 µL)
- Type 2 - Full Kit (Hydrogel+Supplements): VitroGel AAK (2 mL), AAK Supplement 1 (2 x 500 µL)
- Type 3 - Full Kit (Hydrogel+Supplements): VitroGel AAK (2 mL), AAK Supplement 2 (2 x 500 µL)
VitroGel Angiogenesis Assay Kit
- Xeno-free hydrogel system supporting angiogenesis process
- Multiple applications in one kit: Tube formation, invasion, and animal injection
- Control the hydrogel properties: Add your own growth factors and compare with positive control
- Easy, efficient, and enzyme-free cell harvesting in 20 minutes
VitroGel® Angiogenesis Assay Kit is a revolutionary tool for researchers to study the effect of both hydrogel properties and culture medium on angiogenesis process. The kit can be used to study the angiogenesis tube formation and invasion on both 2D hydrogel coating method and 3D cell culture method. The VitroGel® system is also good for animal injection for in vivo study.
Angiogenesis is a highly regulated process that involves the growth of new blood vessels from the existing vasculature. This process plays an important role in both normal developmental processes and numerous pathologies, including wound healing, tumor growth, and metastasis to inflammation and ocular disease.
Traditional angiogenesis assay highly relies on natural extracellular matrix (natural ECM), which has non-adjustable hydrogel compositions and properties. Therefore, our understanding of the angiogenesis process is limited by studying the molecular cues such as growth factors and inhibitors in culture medium only. There is a lack of knowledge on how the properties of hydrogel affect the angiogenesis process.
There are two versions of VitroGel® Angiogenesis Assay Kits:
- VitroGel® Angiogenesis Assay Kit (Ready-To-Use): Ready-to-use with a fixed hydrogel mechanical strength to support the angiogenesis assay with adjustable supplements
- VitroGel® Angiogenesis Assay HC Kit (Cat No. TWG011-K): Assay kit with a tunable high concentration hydrogel to allow full control of the hydrogel’s mechanical strength with adjustable supplements.
Depending on the kit type, the ready-to-use VitroGel®
Angiogenesis Assay Kits can contain the following components:
- VitroGel® AAK, a xeno-free ready-to-use hydrogel.
- AAK Supplement 1, a hydrogel supplement without vascular endothelial growth factors (VEGFs) for cell attachment and growth.
- AAK Supplement 2, a hydrogel supplement with VEGFs to promote tube formation and as a positive control.
The VitroGel® AAK hydrogel is room temperature stable and
can be directly mixed with each supplement at 2:1 (v/v) ratio for hydrogel
formation. Researchers can adjust the
hydrogel’s molecular cues by adding the growth factors/inhibitors directly to
the supplements before mixing with the VitroGel® AAK hydrogel. Cells cultured in this system can be
harvested further easily with the VitroGel® Cell Recovery Solution.
Contents and Specifications
|
TYPE
1 Kit Contents |
VitroGel® AAK
(2 mL) |
|
AAK
Supplement 1 (1 x 500 µL) |
|
|
AAK
Supplement 2 (1 x 500 µL) |
|
|
TYPE
2 Kit Contents |
VitroGel® AAK
(2 mL) |
|
AAK
Supplement 1 (2 x 500 µL) |
|
|
TYPE
3 Kit Contents |
VitroGel® AAK
(2 mL) |
|
AAK
Supplement 2 (2 x 500 µL) |
|
|
Formulation |
Ready-to-use,
xeno-free functional hydrogel |
|
AAK
Supplement 1: Without VEGFs |
|
|
AAK
Supplement 2: With VEGFs |
|
|
Use |
Angiogenesis
Assay, tube formation, invasion, animal injection |
|
Biocompatibility |
Biocompatible,
safe for animal studies |
|
Injection |
Injectable
hydrogel for in vivo studies and lab automation |
|
Cell
Harvesting |
Easy
cell recovery using VitroGel® Cell Recovery Solution |
|
pH |
Neutral |
|
Shipping |
Supplements
require dry ice shipment. |
|
Storage |
VitroGel® AAK
hydrogel: store at 2-8°C |
|
AAK
Supplement 1 and AAK Supplement 2: store at -20°C |
|
|
Number
of Uses |
60
uses at 50 µL per well |
TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.
TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.
For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.
VitroGel® ORGANOID
ready-to-use, xeno-free (animal origin-free) hydrogel system
for organoid culture
- Xeno-free hydrogel for organoid formation
- Supports a wide range of organoids from patient-derived samples, stem cells, tissues, co-culture and PDX resources
- Scalable protocol for lab automation
- Easy 20-minute cell harvesting (enzyme-free)
- VitroGel® ORGANOID (1-4) are xeno-free (animal origin-free) hydrogels that support the growth of patient-derived organoids or organoids developed from pluripotent stem cells (PSCs), co-culture, and PDX model.
VitroGel® ORGANOID hydrogels are ready to use at room temperature and have a neutral pH, transparent, permeable, and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. VitroGel® ORGANOID hydrogels are good for both 3D cell culture and 2D hydrogel coating applications.
The VitroGel® ORGANOID hydrogels can work in conjunction with VitroGel® STEM (a xeno-free hydrogel for 3D static suspension cultures and scale-up of human pluripotent stem cells) by transferring stem cell spheroids cultured in VitroGel® STEM to VitroGel® ORGANOID hydrogels for organoid differentiation. See Xeno-free 3D Organoid Workflow B. Key growth factors and molecules can be directly mixed with the hydrogel matrix or by adding on the top of the hydrogel. Organoids cultured in this system can be easily harvested out with our VitroGel® Cell Recovery Solution. VitroGel® ORGANOID hydrogels provide a well-defined 3D microenvironment for the future of personalized medicine.
Which organoid hydrogel to choose
We still strongly suggest starting with the VitroGel®
ORGANOID Discovery Kit (Item # VHM04-K) to make a quick screening to find the
hydrogel version that is optimal for the organoid conditions.
The kit includes all four types of organoid hydrogels:
VitroGel® ORGANOID-1, VitroGel® ORGANOID-2, VitroGel® ORGANOID-3, VitroGel® ORGANOID-4)
Each of the four types has various bio-functional ligands,
mechanical strengths, and degradability to fulfill the needs of different
organoid culture conditions.
Because there are a wide range of organoid cell resources
(from stem cells, patient-derived tissue, co-culture, to PDX), it is hard to
tell which organoid hydrogel would best perform the researcher’s experiment.
Therefore, the Discovery Kit helps researchers to screen the four different
formulations to determine the best organoid hydrogel version for the organoid
conditions.
From our findings, versions 1, 2, or 3 are suitable for
gastric organoids. Versions 1 or 3 are suitable for lung organoids. Versions 2
or 3 are suitable for brain organoids, and versions 3 or 4 are suitable for
cancer organoids.
Specifications
|
Formulation |
Xeno-free,
functional hydrogel |
|
Use |
Organoid
culture |
|
Operation |
Ready-to-use
at room temperature |
|
Biocompatibility |
Biocompatible,
safe for animal studies |
|
Injection |
Injectable
hydrogel for in vivo studies and lab automation |
|
Cell
Harvesting |
Easy cell recovery using VitroGel® Cell Recovery Solution |
|
pH |
Neutral |
|
Storage |
Store
at 2-8°C. Ships at ambient temperature |
|
Sizes |
Single
Vial: 10 mL |
|
Discovery
Kit: (2 mL) each of ORGANOID-1, ORGANOID-2, ORGANOID-3, ORGANOID-4 |
|
|
Number
of Uses |
(10
mL) 300 uses at 50 µL per well |
|
(2
mL) 60 uses at 50 µL per well |
TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.
TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.
To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.
For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.
AAK Supplement
Hydrogel supplements for use with the VitroGel® Angiogenesis Assay Kits
- AAK Supplement 1 - Without VEGFs (Dry Ice Shipping Required)
- AAK Supplement 2 - With VEGFs (Dry Ice Shipping Required)
Specifications
|
Formulation |
AAK
Supplement 1: |
|
Without
VEGFs |
|
|
AAK
Supplement 2: |
|
|
With
VEGFs |
|
|
Use |
Angiogenesis
Assay, tube formation, invasion, animal injection |
|
Storage |
Store
at -20°C |
|
Shipping |
Requires
dry ice shipment. |
TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.
TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.
To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.