PR1MA™ X-GAL [5-Bromo-4-chloro-3-indolyl-β-D-galactoside]

Common Name: 5-Bromo-4-chloro-3-indolyl-β-D-galactoside
CAS Number: 7240-90-6
Molecular Weight: 408.6
Chemical Formula: C₁₄H₁₅BrCINO₆
Solubility: DMSO and DMF
Storage Temp: -20°C

Chromogenic substrate of β-Galactosidase used in combination with IPTG for detection of β-Galactosidase activity in bacterial colonies. X-GAL is cleaved at the B1-4 bond between galactose and the 5-Bromo-4-chloro-3-indolyl portion of X-GAL by β-Galactosidase via hydrolysis. The cleavage of X-GAL results in the production of a water insoluble blue dichloro-dibromo-indigo precipitate. In cloning strategies with vectors such as Lambda-11, M13mp18 and 19, pUC18 and 19, pUR222 the E. coli lacZ gene is transformed to lac+ cells. After transformation the cells show β-Galactosidase activity in the presence of media containing X-GAL and IPTG. The insertion of a DNA fragment into the cloning sites of the lacZ gene results in the disruption of β-Galactosidase activity leading to the appearance of white colonies. Non-recombinant cells produce a blue indigo dye.

Water Content: 0.05% (K. Fisher)
Protect from light and moisture

Research or further manufacturing use only, not for food or drug use.

• Appearance: White Powder
• Identity: Conforms to structure
• Purity (HPLC): ≥ 98.0 %
• Solubility (2%, DMF): Clear and Colorless Solution
• Specific Rotation: -63.0 - -60.0 °
• Water Content (K.F): ≤ 0.5 %

PR1MA™ IPTG [Isopropyl-β-D-thiogalactopyranoside], Dioxane Free

Common Name: Isopropyl-β-D-thiogalactopyranoside
CAS Number: 367-93-1
Molecular Weight: 238.3
Chemical Formula: C₉H₁₈O₅S
Solubility: Water, ethanol
Storage Temp: 2-8°C

IPTG is combined with X-GAL to detect lac+ colonies or cells in a colorimetric assay. Distinguishes recombinants (white) from non- recombinants (blue) in cloning strategies using vectors such as Lambda-11, M13mp 18 and 19, pUC18 and 19, pUR222 containing the lacZ gene.

Dioxane-Free
Water Content: <0.49%

Research or further manufacturing use only, not for food or drug use.

• Appearance: White Crystalline Powder
• Assay (HPLC): 98.0 - 101.0 %
• Purity (HPLC): ≥ 99.0 %
• 1,4-Dioxane: Not Detected
• Absorbance @300nm (5% w/v sol in H₂O): ≤ 0.13
• Absorbance @400nm (5% w/v solution in H₂O): ≤ 0.06
• Identification: IR Concordant with Standard
• Melting Range: 110 - 114 °C
• Moisture Content (KF): ≤ 1.0 %
• pH (5% in water): 5.0 - 7.0
• Solubility: Soluble in Water and Methanol
• Specific Optical Rotation: -34.5 - -28.5 ^o
• BSE/TSE Free: BSE/TSE Free
• Non Animal Origin: Non Animal Origin
• Country of Origin: Report