Azure HRP Stripping Buffer

Works by removing the primary and secondary antibodies from the membrane

Stripping buffer for stripping and re-probing chemiluminescent Western blots.

• Provided as a 1x ready-to-use solution
• Size: 500mL

Azure Transfer Buffer

This proprietary transfer buffer provides improved detection of low-abundance and post-translationally modified proteins.

High-efficiency protein transfer and increased protein retention on the membrane add up to more sensitive Western blots.

• Provided as a 50x concentrated solution. Sufficient for 50-100 blots
• Size: 500mL

Features

• High performance—increased protein retention on the membrane and fast transfer times result in more sensitive detection of low-abundance and post-translationally modified proteins
• Efficient—enhanced transfer efficiency for proteins of all sizes
• Fast—transfer proteins in less than 20 minutes vs. several hours required with traditional transfer buffers
• Convenient—use your existing wet transfer electrophoresis apparatus

Azure Chemi Blot Blocking Buffer

This Chemi Blot Blocking Buffer was formulated to enhance signal and lower background by improving sensitivity. Enhances specific antibody-antigen interactions specifically for chemiluminescent Western blots. 

• Provided as a 1x ready-to-use solution- no mixing or dilutions required.
• Size: 500mL

Features

Western blot membranes should be incubated in blocking buffer before antibody hybridization to prevent non-specific binding of antibodies. Blocking buffer can also be used for diluting primary and secondary antibodies during hybridization.

Azure Blot Washing Buffer

Wash buffers are an important part of the blotting process for removing unhybridized antibody and preventing background signal.

Blot washing buffer for use with Azure Imaging Systems, or any other Western blotting system.

• Provided as a 10x concentrated solution
• Size: 500mL

NOTE: While this Blot Washing Buffer is compatible with NIR Fluorescent Western blots, for the best results with NIR fluorescence, we recommend the Azure Fluorescent Blot Washing Buffer.

Azure Protein-free Blot Blocking Buffer

Protein-free blocking solution optimized for fluorescent Immunoblots and Western blots

Does not contain proteins. Azure Protein-Free Blot Blocking Buffer can reduce background in Western blots where the primary antibodies tend to cross-react with protein blockers like BSA, casein or milk protein. For low-quality primary antibodies that may require protein-based blocking agents, BSA or non-fat dry milk can be dissolved directly in the Azure Protein-Free Blot Blocking Buffer and then used to dilute the antibody.

Best used with assays where animal-based product usage is prohibited. Serves as an excellent blocking reagent for chemiluminescent Western blotting.

• Provided as a 5x solution
• Size: 200mL