S2K Commerce - Products Dropdown
Actions
S2K Commerce - Shopping Cart
Actions
S2K Commerce - Order Entry
Actions
Need great (q)PCR Regents at a great price?
Try PR1MA! Click here to order.
Bullseye PREMIUM HS-Taq DNA Polymerase Master Mix with HS Buffer I
| Cat.No. | Size Reactions | HS Taq, 2X Mix (Buffer I) | Final MgCl2 Conc. |
| BE230301 | 100 | 2X HS Buffer I Mix | 1.5 mM |
| BE230303 | 500 | 2X HS Buffer I Mix | 1.5 mM |
| BE230304 | 1,000 | 2X HS Buffer I Mix | 1.5 mM |
| BE230306 | 2,500 | 2X HS Buffer I Mix | 1.5 mM |
Store at -20°C. For in-vitro laboratory use only
Bullseye HS Taq Polymerase, 2X Mix is a ready-to-use 2.0X master mix. Simply add primers, template, and water to successfully carry out primer extensions and other molecular biology applications.
Bullseye HS Taq Polymerase Mix, the NH4+ buffer system, dNTPs and magnesium chloride are present in HS Taq Pol Mix with HS Buffer I. Each reaction requires 25 µL of the 2.0X reaction mix. Simply add primers, template and water to a total reaction volume of 50 uL.
Bullseye HS Taq Polymerase Mix is a modified form of Bullseye Taq DNA Polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during setup and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. The result is higher specificity and greater yields when compared to standard DNA polymerases.
Bullseye" HS Taq Polymerase Mix offers several advantages. Set up time is significantly reduced. The chance of contaminating component stocks is eliminated. Reduction of reagent handling steps leads to better reproducibility. Standard tests can be set up with the confidence that results will be consistent every time.
Composition of HS Taq Pol, 2x Mix
Tris-HCl pH 8.5, (NH4)2S04, 3.0mM MgCl2, 0.2% Tween 20Ã, 0.4 mM dNTPs, 0.2 units/uL HS Hot Start DNA Polymerase Stabilizer
Suggested Protocol using HS Taq Pol, 2x Mix
This protocol serves as a guideline only. Optimal reaction conditions may vary and must be individually determined.
-
Set up reaction mixtures in an area separate from that used for DNA preparation or product analysis.
-
The table below shows the reaction set up for a final volume of 50 mL.
-
Important: Mix the solutions completely before use to avoid localized concentrations of salts.
1. Set up each reaction as follows:
| Component | Vol./Reaction | Final Conc. |
| HS Hot Start Master Mix w/ HS Bufffer I | 25 uL | 1X |
| Primer A | Variable | 0.11.0 uM |
| Primer B | Variable | 0.11.0 uM |
| Distilled Water | Variable | - - - - |
| Template DNA | Variable | Variable |
| TOTAL volume | 50 uL | - - - - |
2. Mix gently by pipetting the solution up and down a few times.
3. Program the thermal cycler according to the manufacturer's instructions.
4. Each program must start with an initial heat activation step at 95°C for 15 minutes.
For maximum yield and specificity, temperatures and cycling times should be optimized for each new template target or primer pair.
A typical thermal cycling program is shown below:
95°C for 15 min. Activate HS Hot Start Polymerase
30-40 cycles:
95°C 30 sec Denature template
45-65°C 30 sec Anneal primer
72°C 1-5 min Elongation
72°C for 5 min Elongation
5. Place the tubes in the thermal cycler and start the reaction.
This brand is being discontinued and will only be available while supplies last.
Need great (q)PCR Regents at a great price?
Try our PR1MA™ Taq and save!
Bullseye PREMIUM Std Taq
- Excellent all-purpose amplification enzyme
- Thermostable recombinant DNA polymerase from Thermus aquaticus
- Exhibits very high activity in primer extension
- Has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity
- Does not have 3' to 5' exonuclease activity-no proofreading ability
- Leaves an A-overhang, which makes the enzyme ideal for TA cloning
- Includes MgCl² in buffer
This brand is being discontinued, please contact us for other options.
Looking for other alternatives?
Visit our PR1MA Polymerase page for more options!
Bullseye PREMIUM Taq DNA Polymerases
Store at -20°C. For in-vitro laboratory use onlyBullseye Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa.
Bullseye Taq DNA Polymerase has both a 5'®3' DNA polymerase and a 5'®3' exonuclease activity. The enzyme lacks a 3'®5' exonuclease activity (no proofreading ability). Taq DNA Polymerase leaves an A¢ overhang, which makes the enzyme ideal for TA cloning.
The 10X Reaction Buffer provided does not contain Mg+2. 25 mM MgCl2 is supplied separately.
10X Mg++ Free Standard Buffer
100 mM Tris-HCl pH 8.3, 500 mM KCl.
Unit Definition
One unit is defined as the amount that incorporates 10 nmoles of dNTPs into acid-precipitable form in 30 minutes at 72°C under standard assay conditions.
Storage and Dilution Buffer
Enzyme is supplied in 20 mM Tris-HCl pH 8.3, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Tween 20, 0.5%NP40, 50% glycerol.
Quality Control
Each lot of Taq DNA Polymerase is tested for contaminating activities, with no trace of endonuclease activity, nicking activity, exonuclease activity or priming activity.
Suggested Protocol using Taq DNA Polymerase
This protocol serves as a guideline only. Optimal reaction conditions may vary and must be individually determined.
n In some applications, MgCl2 is needed for the best results. For this reason, 25 mM MgCl2 is included with the kit.
1. Thaw 10X Mg2+ Free Standard Buffer, dNTP mix, primer solutions. It is important to mix the solutions completely before use to avoid localized concentrations of salts.
2. Prepare a master mix according to Table 1. The master mix typically contains all the components needed for extension except the template DNA.
Table 1. Reaction components (master mix & template DNA)
| Component | Vol./reaction | Final Conc. |
| 10X Mg2+ Free Buffer | 5 uL | 1X |
| MgCl2, 25 mM | 1- 9 uL | 0.5 4.5 mM |
| dNTP mix (12.5 mM of each) | 0.8 uL | 0.2 mM of each dNTP |
| Primer A | Variable | 0.11.0 uM |
| Primer B | Variable | 0.11.0 uM |
| Taq DNA Polymerase | Variable | 1-5 units |
| Template DNA | Variable | Variable |
| Distilled Water | Variable | - - - - |
| TOTAL volume | 50 uL | - - - - |
Table 2. MgCl2 concentration in a 50 mL reaction
| Final MgCl2 conc. in reaction (mM) | 0.5 | 1 | 1.5 | 2 | 2.5 | 3 | 3.5 | 4 | 4.5 |
| Vol. of 25 mM MgCl2 per rxn (uL) | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 |
Table 2 provides the volume of 25 mM MgCl2 to add to the master mix if a certain MgCl2 concentration is required.
3. Mix the master mix thoroughly and dispense appropriate volumes into reaction tubes. Mix gently, e.g., by pipetting the master mix up and down a few times.
4. Add template DNA to the individual tubes containing the master mix.
5. Program the thermal cycler according to the manufacturers instructions. For maximum yield and specificity, temperatures and cycling times should be optimized for each new template target or primer pair.
6. Place the tubes in the thermal cycler and start the reaction.
This brand is being discontinued, please contact us for other options.
Looking for other alternatives?
Visit our PR1MA Polymerase page for more options!
Bullseye PREMIUM R-Taq DNA Polymerase
R-Taq DNA Polymerase 5 units/µL
| Item # | Units | 10X Ammonium Buffer (MgCl2 15mM) | MgCl2 25 mM |
| BE200303 | 500 | 1.5 mL | 1.5 mL |
| BE200304 | 1,000 | 2x 1.5 mL | 2x 1.5 mL |
| BE200306 | 2,500 | 4x 1.5 mL | 4x 1.5 mL |
Store at -20°C. For in-vitro laboratory use only
Bullseye R-Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. R-Taq contains a red dye which provides easy and quick identification of reactions to which enzyme was added and allows confirmation of complete mixing. The inert dye has no effect on downstream processes. R-Taq is added directly to the reaction mix and is used in the same manner as standard Taq DNA Polymerase.
Bullseye R-Taq DNA Polymerase has both a 5'®3' DNA polymerase and a 5'®3' exonuclease activity. The enzyme lacks a 3'®5' exonuclease activity. R-Taq DNA Polymerase leaves an A-overhang, which makes the enzyme ideal for TA cloning.
- High performance thermostable DNA polymerase
- Red dye identifies tubes which contain enzyme and confirms complete mixing of reagents
- Leaves an A-overhang
Unit Definition
One unit is defined as the amount that incorporates 10 nmoles of dNTPs into acid-precipitable form in 30 minutes at 72°C under standard assay conditions.
Storage Buffer
Enzyme is supplied in 20 mM Tris-HCl pH 8.3, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, inert dye, 0.5 % Tweenà 20, 0.5% NP40, 50% glycerol.
| Component | Vol./reaction | Final Conc. |
| 10X Ammonium Buffer | 5 uL | 1X |
| dNTP mix (12.5 mM each) | 0.8 uL | 0.2 mM each dNTP |
| Primer A | Variable | 0.1-0.5 uM |
| Primer B | Variable | 0.1-0.5 uM |
| R-Taq DNA Pol | 1 mL | 5 units/reaction |
| Distilled Water | Variable | - - - - |
| Template DNA | Variable | 0.1-0.5 uG/reaction |
| TOTAL volume | 50 uL | - - - - |
Table 2. MgCl2 concentration
3. Mix the master mix thoroughly and dispense appropriate volumes into reaction tubes. Mix gently (e.g., by pipetting) the master mix up and down a few times.
4. Add template DNA (0.1-0.5 mg/reaction) to the individual tubes containing the master mix.
5. Program the thermal cycler according to the manufacturer's instructions.
For maximum yield and specificity, temperatures and cycling times should be optimized for each new template target or primer pair.
6. Place the tubes in the thermal cycler and start the reaction.
7. After primer extension, load 5-10 mL of a 50 mL reaction directly on an agarose gel for analysis.
Tween 20 is a registered trademark of ICI Americas, Inc.
| Final MgCl2 conc. in reaction (mM) | 1.5 | 2 | 2.5 | 3 | 3.5 | 4 | 4.5 |
| Vol. of 25 mM MgCl2 / rxn (uL) | 0 | 1 | 2 | 3 | 4 | 5 | 6 |
10X Ammonium Reaction Buffer
Tris-HCl pH 8.5, (NH4)2S04, 15 mM MgCl2, 1% Tween 20
Quality Control
Endonuclease, exonuclease and priming activities are not detected after 3 hours incubation of 1 mg of pUC19 plasmid DNA and 0.5 uG EcoR I digested lambda phage DNA at 72°C in the presence of 40 units of R-Taq DNA Polymerase.
Suggested Protocol using R-Taq Polymerase
This protocol serves as a guideline. Optimal reaction conditions such as incubation times, temperatures, and amount of template DNA may vary and must be individually determined.
1. Thaw 10X Ammonium Buffer, dNTP mix, and primer solutions. It is important to mix the solutions completely before use to avoid localized concentrations of salts.
2. Prepare a master mix according to Table 1. The master mix typically contains all the components needed for extension except the template DNA.
The optimal MgCl2 concentration should be determined empirically but, in most cases a concentration of 1.5 mM, as provided in the 1X Ammonium Buffer, produces satisfactory results. Table 2 provides the volume of 25mM MgCl2 to add to the master mix if a higher MgCl2 concentration is required.
Table 1. Reaction components (master mix & template DNA)
PR1MA™ Benzamidine Hydrochloride
CAS Number: 1670-14-0
Molecular Weight: 156.6
Chemical Formula: C7H8N2 • HCl
Solubility: Water
Storage Temp: 2-8°C
Competitive serine protease inhibitor which forms stable complexes with and blocks the active sites of enzymes. Target enzymes include trypsin, hymotrypsin, kallikzein and plasmin.
Shipping Weight: 0.3 lbs
Shipping Dimensions: 3.50 x 3.75 x 3.50
Research or further manufacturing use only, not for food or drug use.
Specifications
- Appearance: White crystalline powder
- Assay (HPLC): ≥ 98.5 %
- Loss on Drying: ≤ 2 %
- Residue on Ignition: ≤ 0.5 %
- Material Source: Chemical Synthesis
- BSE/TSE Free: BSE/TSE Free
- Country of Origin: Report
MagBio HighPrep™ Viral/Pathogen DNA/RNA Kit
Magnetic beads based kit for rapid isolation of viral, bacterial and fungal nucleic acids from whole blood, serum, plasma, saliva and other body fluids.
- RT-qPCR; RT-PCR, PCR
- One-Step RT-qPCR
- Virus detection, genotyping
- Viral load monitoring,
- OPTIMIZED FOR ISOLATION from FUNGAL, BACTERIAL and VIRAL samples.
- Rapid and reliable purification of nucleic acids
- Adaptable to various automated liquid handling workstations
- No toxic organic solvents
The HighPrep™ Viral/Pathogen DNA/RNA kit is designed for rapid and reliable isolation of viral, bacterial and fungal nucleic acids from whole blood, serum, plasma, saliva and other body fluids as well as nasopharyngeal swabs soaked in virus transport media or other buffers. This kit is highly efficient in vIral nucleic acid isolation and the extracted RNA (and DNA) is suitable for direct use in most downstream applications such as one-step RT-qPCR, RT-PCR, PCR, nucleic acid amplification, cloning, sequencing, and enzymatic reactions. The kit can be used in low throughput manual workflows and is also adaptable to majority of the liquid handling workstations in the market.
, Beads
PCR DNA Clean-up Kit
The 96-well PCR Clean-Up/DNA Extraction Kits provide a high-throughput, rapid and economical method to purify DNA fragments. Chaotropic salt is used to denature enzymes and in this condition, DNA fragments are bound by the glass fiber matrix in each well of the plate. Once the contaminants have been removed, the purified DNA is eluted by a low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides are effectively removed from reaction mixtures without toxic phenol extraction or alcohol precipitation. This entire protocol can be completed in 30-40 minutes, and the eluted DNA is ready to use in restricted digestion, ligation, PCR, and sequencing reactions.Specifications
| Sample Size | Up to 50 uL of PCR product Up to 50 mg of agarose slice |
| Format | 96-Well Plates |
| Operation | Centrifuge/Vacuum manifold |
| Binding Capacity | 10 uG per well |
| DNA Size | 50 bp - 10 kb |
| Recovery | 80-90% for Gel Extraction 90-95% for PCR Clean-Up |
| Operation Time | 30 min for PCR Clean-Up 40 min for Gel Extraction |
PCR DNA Clean-up Kit
The 96-well PCR Clean-Up/DNA Extraction Kits provide a high-throughput, rapid and economical method to purify DNA fragments. Chaotropic salt is used to denature enzymes and in this condition, DNA fragments are bound by the glass fiber matrix in each well of the plate. Once the contaminants have been removed, the purified DNA is eluted by a low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides are effectively removed from reaction mixtures without toxic phenol extraction or alcohol precipitation. This entire protocol can be completed in 30-40 minutes, and the eluted DNA is ready to use in restricted digestion, ligation, PCR, and sequencing reactions.Specifications
| Sample Size | Up to 50 uL of PCR product Up to 50 mg of agarose slice |
| Format | 96-Well Plates |
| Operation | Centrifuge/Vacuum manifold |
| Binding Capacity | 10 uG per well |
| DNA Size | 50 bp - 10 kb |
| Recovery | 80-90% for Gel Extraction 90-95% for PCR Clean-Up |
| Operation Time | 30 min for PCR Clean-Up 40 min for Gel Extraction |
MagBio HighPrep™ Insect DNA Kit
Magnetic beads based kit designed to extract high-quality genomic DNA from insects, and arthropods (from one leg or wing to the whole body).
- PCR, Real-time PCR
- Cloning, genotyping
- Sequencing
- Insecticide resistance studies
- Efficient isolation of PCR-quality DNA from insects, and arthropods
- No organic solvents
- Allows field epidemiologic research
- Magnetic beads based chemistry adaptable to automation
The HighPrep™ Insect DNA Kit is a high quality paramagnetic bead-based genomic DNA extraction kit used to isolate gDNA from a variety of insect-derived materials (wings, legs, whole body), and from different insect species, such as mosquitoes, flies, ants, and others. The HighPrep™ Insect DNA Kit is based on a fast and simple procedure which can either be performed manually or automated - up to 96 samples of tissues including legs and wings, can be processed in less than an hour.
, Beads
MagBio HighPrep™ RNA Elite Clean-up System
Magnetic beads based reagent for manual and automated clean-up or concentration of RNA (including miRNA, siRNA, aRNA) and single-stranded cDNA after enzymatic reactions or prior sequencing.
- cDNA synthesis
- RT-PCR
- Sequencing
- In vitro transcription
- RNA probe synthesis
- miRNA and siRNA preparation
- Rapid and reliable clean-up of ss cDNA and RNA, including miRNA, siRNA, and aRNA
- Efficient purification of both large and small RNAs, and cDNA
- Complete removal of salts, unincorporated primers, and nucleotides
- No centrifugation step, no filtration step
- Adaptable to your current liquid handling workstation
, Beads
MagBio cfKapture™ Kit
Magnetic bead-based DNA extraction kit designed to isolate circulating cell free DNA (cfDNA) from human plasma and serum.
- Liquid biopsy research
- NIPT genomic research
- Cancer genomic research
- Transplant monitoring
- Efficient Isolation of nucleic acids, with high input and low elution volumes
- No carrier RNA
- Low sample volume processing
- Efficient recovery of small sized fragmented DNA
- No organic extraction or ethanol precipitation
, Beads
MagBio HighPrep™ Plant RNA Plus Kit
Magnetic beads based kit designed to extract high-quality RNA from plants (leaves, roots, seeds).
- PCR, Real-time PCR
- Cloning, genotyping
- Sequencing
- Plant breeding
- Purified PCR grade RNA with no inhibiting substances (polysaccharides and phenols)
- Includes beads to homogenize sample
- Eliminate need of grinding
- Adaptable to automation
, Beads
Web Content Viewer
Actions