PR1MA™ Malt Extract Agar, Powder

CAS Number: 8002-48-0
Solubility: Water with Heat
Storage Temp: Room Temperature

Malt Extract Agar is used for the isolation, cultivation, and maintenance of yeasts and molds. Formulation is similar to Malt Extract Broth with the addition of agar and glycerol. This broth medium was recommended by Thom and Church for the cultivation of fungi. Its low acidity inhibits the growth of most bacteria. The high carbohydrate content enhances the rapid growth of fungi and yeasts.

Dissolve 33.6 grams in 1 liter of distilled water. Sterilize by autoclaving for 15 minutes.

Ingredients:

• Maltose: 12.75 g/L
• Dextrin: 2.75 g/L
• Glycerol: 2.35 g/L
• Peptone: 0.78 g/L
• Agar: 15.0 g/L
• Final pH (25°C): 4.6 ± 0.2

Research or further manufacturing use only, not for food or drug use.

• DCM Appearance: Homogeneous with soft lumps, free flowing, light beige
• PPM Appearance: Trace to moderate haze, off white to pale yellow
• pH(3.36% Solution @ 25 ^oC: 4.5 - 4.9
• Microorganism (Aspergillus niger ATCC 16404): Growth
• Microorganism (Candida albicans ATCC 10231): Growth
• Microorganism (Penicillium Roquefortii ATCC 10110): Growth
• Microorganism (Saccharomyces cerevisiae ATCC 9763): Growth
• Microorganisms (Microsporum Audouinii ATCC 42558): Growth
• Microorganisms (Microsporum Canis ATCC 36299): Growth
• Microorganism (Trichophyton Mentagrophytes ATCC 9533): Growth

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PR1MA™ Yeast Extract, Powder

CAS Number: 8013-01-2
Solubility: Water
Storage Temp: Room Temperature

Dried yeast autolysate with a high content of amino nitrogen and water soluble B complex vitamins. Due to its low NaCl content this quality is well suited for tissue culture.

Ingredients:

• Total Nitrogen (TN): ~9.8%
• Amino Nitrogen (AN): ~5.1%
• AN/TN Ratio: ~0.52
• Sodium Chloride: ~0.3%
• pH (1% Solution): ~7.0

• Appearance: Light Beige Powder
• Amino Nitrogen: 4.5 - 5.8 %
• Ash Content: 11.50 - 16.0 %
• Dry Matter: ≥ 95.0 %
• Loss on Drying: ≤ 5.0 %
• pH (2.0% Solution @ 25 ^oC): 6.8 - 7.2
• Sodium Chloride: ≤ 0.5 %
• Total Nitrogen: 10.0 - 11.8 %
• Clostridium Perfringens Spores : Report cfu/g
• E.Coli: Report cfu/g
• Molds and Yeasts: Report cfu/g
• Pathenogenic Staphylococcus: Report cfu/g
• Salmonella : Report cfu/25g
• Standard Plate Count: Report cfu/g

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PR1MA™ 2XYT Broth, Powder

CAS Number: 91079-40-2; 8013-01-2; 7647-14-5
Solubility: Water
Storage Temp: Room Temperature

Nutritionally enriched medium for the growth of recombinant strains of E. Coli and in the preparation and transformation of competent cells.

Dissolve 31g in one liter distilled water. Heat solution to boiling with continuous stirring. Sterilize by autoclaving at 121°C for 15 minutes. Final ph @ 25°C: 6.8-7.2.

Ingredients:

• Digest Peptone: 16 g/l
• Yeast Extract: 10 g/l
• Sodium Chloride: 5 g/l

Research or further manufacturing use only, not for food or drug use.

• Appearance: Cream to beige free flowing powder
• DCM Appearance: Homogeneous free flowing, beige
• PPM Appearance: Clear,no to light ppt,yellow
• pH (3.1 % Solution @25 ^oC): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

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PR1MA™ Peptone, Powder

CAS Number: 73049-73-7
Solubility: Water
Storage Temp: Room Temperature

Mixture of peptides and free amino acids from pancreatic hydrolysis of animal tissue. Low NaCl content suitable for tissue culture.

Total Nitrogen (TN): 15.0-16.0%
Amino Nitrogen (AN): 3.0-3.5%
AN/TN Ratio: 0.21-0.23

Research or further manufacturing use only, not for food or drug use.

• Appearance: White to Light Beige Fine Powder
• Amino Nitrogen: ≥ 3.4 %
• Ash, Residue in Ignition: ≤ 15 %
• Loss on Drying: ≤ 5 %
• Nitrites: Negative
• pH (2.0% Solution @ 25 ^oC): 6.5 - 7.5
• Proteoses: Report
• Total Nitrogen: ≥ 10 %
• Undigested Protein: Negative
• Coliforms: Negative
• E.Coli: Negative
• Molds and Yeasts: Report cfu/g
• Salmonella : Negative cfu/25g
• Standard Plate Count : ≤ 1000 cfu/g
• Broth Formulations (Growth Supporting Properties): Satisfactory
• Peptone Agar (Growth Supporting Properties): Satisfactory
• Raw Material Species: Bovine
• Enzyme Species: Porcine

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PR1MA™ Luria Agar Powder [Miller's LB Agar]

Common Name: Miller's LB Agar
CAS Number: 91079-40-2; 8013-01-2; 7647-14-5; 9002-18-0
Solubility: Water with Heat
Storage Temperature: Room Temperature

For propagation and maintenance of E. Coli. High Salt Formula. Molecular Biology Grade.

• Dissolve 40 grams in 1 liter distilled water
• Adjust pH to 7.2
• Sterilize by autoclaving for 15 minutes

Ingredients:

• Peptone: 10g/L
• Yeast: 5 g/L
• Sodium Chloride: 10g/L
• Agar: 15g/L

Research or further manufacturing use only, not for food or drug use.

• DCM Appearance: Homogeneous Free Flowing, Light Beige
• PPM Appearance: Clear, no to light ppt, light yellow
• pH (4% solution at 25°C): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

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PR1MA™ Tryptone, Powder

CAS Number: 91079-40-2
Solubility: Water
Storage Temp: Room Temperature

Pancreatic digest of casein used to supplement media in cultivation of anaerobic bacteria.

pH (1% solution): 7.3.

Ingredients:

• Molecular Biology GradeTotal Nitrogen (TN): ~12.7%
• Amino Nitrogen (AN): ~3.7%
• AN/TN Ratio: 0.26
• Sodium Chloride: ~0.4%
• Tryptophan: ~1.0

Research or further manufacturing use only, not for food or drug use.

• Appearance: Cream to beige free flowing powder
• Amino Nitrogen: ≥ 4.0 %
• Ash: ≤ 15 %
• Loss on Drying: ≤ 5.0 %
• Nitrites: Negative
• pH (2% water) @25°C: 6.5 - 7.5
• Total Nitrogen: ≥ 10 %
• Undigested Protein: Negative
• Broth Formulations (Growth Supporting Properties): Satisfactory
• Peptone Agar (Growth Supporting Properties): Satisfactory
• Proteoses: Positive
• Salmonella: Negative/25g
• Standard Plate Count : ≤ 1000 cfu/g
• Coliform: Negative
• E.Coli: Negative
• Molds and Yeasts: ≤ 100 cfu/g
• Raw Material Species: Bovine
• Enzyme Species: Porcine
• BSE/TSE Free: BSE/TSE Free
• Country of Origin : Report

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PR1MA™ Sodium Chloride, Powder

CAS Number: 7647-14-5
Molecular Weight: 58.4
Chemical Formula: NaCl
Solubility: Water
Storage Temp: Room Temperature

Research or further manufacturing use only, not for food or drug use.

• Appearance: Fine White Granular Powder
• Acidity or Alkalinity: Pass Test
• Appearance of Solution: Clear, Colorless Solution
• Arsenic : ≤ 1 µg/g
• Assay: 99.0 - 100.5 %
• Barium: Pass Test
• Bromides: ≤ 0.010 %
• Ferrocyanide: Pass Test
• Heavy Metals : ≤ 5 ppm
• Identification (Chloride): Pass Test
• Identification (Sodium): Pass Test
• Loss on Drying: ≤ 0.5 %
• Magnesium & Alkaline Earth Metals:  0.01 %
• Iron(Fe): ≤ 2 µg/g
• Nitrites: ≤ 0.01
• Phosphates : ≤ 0.0025 %
• Sulfate: ≤ 0.020 %

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PR1MA™  BHI Broth [Brain Heart Infusion Broth], Powder

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PR1MA™ Gelrite™

CAS Number: 71010-52-1
Solubility: Water
Storage Temp: Room Temperature

Naturally derived gelling polymer that can be used in place of agar. Gelrite forms clear, rigid gels at approximately half the use level of agar in presence of soluble salts like Mg2+ and Ca2+.

Research or further manufacturing use only, not for food or drug use.

• Appearance: Solid
• Gel Strength: 400 - 700 g/cm²
• Loss on Drying: ≤ 15.0 %
• Particle Size 42 Mesh (355 µm): ≥ 95 %
• Transmittance: ≥ 80.0 %

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PR1MA™ Agar, Phyto, Powder

CAS Number: 9002-18-0
Solubility: Water
Storage Temp: Room Temperature

Specially selected and tested agar for use in plant tissue culture. High gel strength.

Concentration of 5.0 g/l obtains a solid gel strength: (1600 g/cm2).

Research or further manufacturing use only, not for food or drug use.

• Appearance: Pale Yellow Powder
• Gelling Point: 34 - 36 °C
• Gel Strength : 950 - 1050 g/cm²
• Identification: Conforms
• Loss on Drying: ≤ 18 %
• Melting Point: 85 - 95 ^oC
• Nephelos: ≤ 40 NTU
• pH: 6.0 - 7.5
• Total Ash: ≤ 3.5 %
• Gelatin: Absent
• Starch: Absent
• Total Plate Count: ≤ 1000 cfu/g

PR1MA™ Casamino Acids [Casein acid hydrolysate]

Common Name: Casein acid hydrolysate
CAS Number: 91079-40-2
Solubility: Water
Storage Temp: Room Temperature

Casein Acid Hydrolysate is a hydrochloric acid digest of casein that contains small amounts of cystine and Tryptophan. All vitamins have been eliminated by the acidification treatment. Provides a source of amino acids as nutrients for growing various organisms tolerant of high salt conditions.

Research or further manufacturing use only, not for food or drug use.

• Appearance: Homogeneous, Free Flowing Powder
• Color: Light Tan Yellow
• Amino Nitrogen: ≥ 4.0 %
• Moisture : ≤ 6.0 %
• pH: 6.0 - 7.5
• Protein: Report %
• Solution (2%): Clear Light Yellow
• Total Nitrogen: > 7.5 %
• Coliforms: < 10 cfu/g
• Standard Plate Count : < 5000 cfu/g
• Thermophile Count: Report cfu/g
• Source: Bovine

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PR1MA™ Agar, High Gel Strength, Powder

CAS Number: 9002-18-0
Solubility: Water
Storage Temp: Room Temperature

Purified high gel strength agar with excellent properties for tissue culture and microbiological work.

Molecular Biology Grade

Research or further manufacturing use only, not for food or drug use.

• Appearance: White to Cream Powder
• Ash: ≤ 4.5 %
• Gel Point (1.5% Solution): 33 - 38 °C
• Gel Strength (1.5% Gel): ≥ 800 g/cm²
• Loss on Drying: ≤ 12.0 %
• Melting Point (1.5 %): 88.0 - 96.0
• pH (1.5% solution): 6.0 - 8.0