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Molecular Biology Reagents/Chemicals
MIDSCI™ gives you ultra-pure, cost-competitive molecular biology reagents and lab chemicals for life science research; often with significant cost savings over Sigma Aldrich and other lab reagent providers.
PR1MA™ Chemicals can help with a wide variety of research-based requirements.
Use our glycerol for polyacrylamide gel electrophoresis. Eliminate Agrobacterium after inoculation with the carbenicillin we carry. Or detect β-Galactosidase activity with X-GAL which, when combined with the IPTG we stock, can be used to detect lac gene expression in cloning applications.
We also carry Luria Broth (Lennox), for the propagation and maintenance of E. coli; DTT, in order to maintain –SH groups in a reduced state; and high gel strength agar for tissue culture and microbiological work.
This brand is being discontinued, please contact us for other options.
Looking for other alternatives?
Visit our PR1MA Polymerase page for more options!
Bullseye PREMIUM R-Taq DNA Polymerase
R-Taq DNA Polymerase 5 units/µL
| Item # | Units | 10X Ammonium Buffer (MgCl2 15mM) | MgCl2 25 mM |
| BE200303 | 500 | 1.5 mL | 1.5 mL |
| BE200304 | 1,000 | 2x 1.5 mL | 2x 1.5 mL |
| BE200306 | 2,500 | 4x 1.5 mL | 4x 1.5 mL |
Store at -20°C. For in-vitro laboratory use only
Bullseye R-Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. R-Taq contains a red dye which provides easy and quick identification of reactions to which enzyme was added and allows confirmation of complete mixing. The inert dye has no effect on downstream processes. R-Taq is added directly to the reaction mix and is used in the same manner as standard Taq DNA Polymerase.
Bullseye R-Taq DNA Polymerase has both a 5'®3' DNA polymerase and a 5'®3' exonuclease activity. The enzyme lacks a 3'®5' exonuclease activity. R-Taq DNA Polymerase leaves an A-overhang, which makes the enzyme ideal for TA cloning.
- High performance thermostable DNA polymerase
- Red dye identifies tubes which contain enzyme and confirms complete mixing of reagents
- Leaves an A-overhang
Unit Definition
One unit is defined as the amount that incorporates 10 nmoles of dNTPs into acid-precipitable form in 30 minutes at 72°C under standard assay conditions.
Storage Buffer
Enzyme is supplied in 20 mM Tris-HCl pH 8.3, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, inert dye, 0.5 % Tweenà 20, 0.5% NP40, 50% glycerol.
| Component | Vol./reaction | Final Conc. |
| 10X Ammonium Buffer | 5 uL | 1X |
| dNTP mix (12.5 mM each) | 0.8 uL | 0.2 mM each dNTP |
| Primer A | Variable | 0.1-0.5 uM |
| Primer B | Variable | 0.1-0.5 uM |
| R-Taq DNA Pol | 1 mL | 5 units/reaction |
| Distilled Water | Variable | - - - - |
| Template DNA | Variable | 0.1-0.5 uG/reaction |
| TOTAL volume | 50 uL | - - - - |
Table 2. MgCl2 concentration
3. Mix the master mix thoroughly and dispense appropriate volumes into reaction tubes. Mix gently (e.g., by pipetting) the master mix up and down a few times.
4. Add template DNA (0.1-0.5 mg/reaction) to the individual tubes containing the master mix.
5. Program the thermal cycler according to the manufacturer's instructions.
For maximum yield and specificity, temperatures and cycling times should be optimized for each new template target or primer pair.
6. Place the tubes in the thermal cycler and start the reaction.
7. After primer extension, load 5-10 mL of a 50 mL reaction directly on an agarose gel for analysis.
Tween 20 is a registered trademark of ICI Americas, Inc.
| Final MgCl2 conc. in reaction (mM) | 1.5 | 2 | 2.5 | 3 | 3.5 | 4 | 4.5 |
| Vol. of 25 mM MgCl2 / rxn (uL) | 0 | 1 | 2 | 3 | 4 | 5 | 6 |
10X Ammonium Reaction Buffer
Tris-HCl pH 8.5, (NH4)2S04, 15 mM MgCl2, 1% Tween 20
Quality Control
Endonuclease, exonuclease and priming activities are not detected after 3 hours incubation of 1 mg of pUC19 plasmid DNA and 0.5 uG EcoR I digested lambda phage DNA at 72°C in the presence of 40 units of R-Taq DNA Polymerase.
Suggested Protocol using R-Taq Polymerase
This protocol serves as a guideline. Optimal reaction conditions such as incubation times, temperatures, and amount of template DNA may vary and must be individually determined.
1. Thaw 10X Ammonium Buffer, dNTP mix, and primer solutions. It is important to mix the solutions completely before use to avoid localized concentrations of salts.
2. Prepare a master mix according to Table 1. The master mix typically contains all the components needed for extension except the template DNA.
The optimal MgCl2 concentration should be determined empirically but, in most cases a concentration of 1.5 mM, as provided in the 1X Ammonium Buffer, produces satisfactory results. Table 2 provides the volume of 25mM MgCl2 to add to the master mix if a higher MgCl2 concentration is required.
Table 1. Reaction components (master mix & template DNA)
PR1MA™ TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane HCl]
Common Name: Tris(hydroxymethyl) aminomethane hydrochloride
CAS Number: 1185-53-1
Molecular Weight: 157.6
Chemical Formula: C4H11NO3 • HCl
Solubility: Water
Storage Temp: Room Temperature
Highly purified for use in molecular biology applications.
Research or further manufacturing use only, not for food or drug use.
- Appearance: White crystalline
- Assay: 99.0 - 101.0 %
- pH (1% aqueous solution): 4.2 - 5.0
- Solubility (1M Solution in Water ) : Clear, colorless solution
- UV Absorbance @260nm, 1.0 M in H2O: ≤ 0.06
- UV Absorbance @280nm, 1.0 M in H2O: ≤ 0.05
- Identification: Conforms
- Heavy Metals : ≤ 5 ppm
- Grade: USP
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PR1MA™ Nystatin
CAS Number: 1400-61-9
Molecular Weight: 926.1
Chemical Formula: C47H75NO17
Solubility: DMSO
Storage Temp: 2-8°C
Polyene antifungal antibiotic produced by Streptomyces noursei.
Research or further manufacturing use only, not for food or drug use.
- Appearance: Yellow to light tan powder
- Crystallinity: Pass Test
- Identification: Pass Test
- Loss on Drying: ≤ 5.0 %
- pH @25 °C: 6.0 - 8.0
- Potency (Assay): ≥ 4400 U/mg
- Suspendability: Pass Test
- Residual Solvent (n-butanol): ≤ 1000 ppm
- Residual Solvent (Methanol): ≤ 3000 ppm
- Residual Solvent (Acetone): ≤ 5000 ppm
- Residual Solvents: The following Class 1,2, or 3 Solvents may be present in this product. All comply with the limits prescribed in the current USP.
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PR1MA™ MES Monohydrate [2-(N-Morpholino)-ethanesulfonic acid]
Common Name: 2-(N-Morpholino)ethanesulfonic acid
CAS Number: 145224-94-8
Molecular Weight: 213.24
Chemical Formula: C6H13NO4S • H 2O
Solubility: Water
Storage Temp: Room Temperature
Buffer for use in plant tissue culture.
- pKa (20°C): 6.15
- pH range: 5.5 - 6.7
Research or further manufacturing use only, not for food or drug use.
- Appearance: White crystalline powder
- Assay (Titration): ≥ 99.0 %
- Heavy Metals: ≤ 0.0010 %
- Loss on Drying: 7.0 - 10.0 %
- pH: 2.5 - 4.0
- pKa @20 oC: 6.02 - 6.25
- Solubility (10% solution in H2O): Clear
- Sulfate: ≤ 0.01 %
- UV Absorbance 250nm in 10% H2O: ≤ 0.05
- Country of Origin: Report
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PR1MA™ Potassium Acetate
CAS Number: 127-08-2
Molecular Weight: 98.14
Chemical Formula: C2H3O2K
Solubility: Water
Storage Temp: Room Temperature
Research or further manufacturing use only, not for food or drug use.
- Appearance: White crystalline powder
- Calcium (Ca): ≤ 0.005 %
- Chloride (Cl): ≤ 0.003 %
- Heavy Metals : ≤ 5 ppm
- Insolubles: ≤ 0.005 %
- Iron: ≤ 5 ppm
- Magnesium (Mg): ≤ 0.002 %
- pH (5% in water): 6.5 - 9.0
- Phosphate: ≤ 0.001 %
- Purity: ≥ 99.0 %
- Sodium (Na): ≤ 0.03 %
- Sulfate: ≤ 0.002 %
- Country of Origin: Report
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PR1MA™ Blasticidin S Hydrochloride 10 mg / mL Solution
CAS Number: 3513-03-9
Molecular Weight: 458.9
Chemical Formula: C17H26N8O5 • HCl
Solubility: Water
Storage Temp: -20°C
A peptidyl nucleoside antibiotic isolated from the culture broth of Streptomyces griseogenes, inhibits protein synthesis in both prokaryotes and eukaryates.
Supplied as a 10 mg / mL sterile solution in HEPES buffer.
Research or further manufacturing use only, not for food or drug use.
- Appearance: Clear Solution
- Concentration: 10mg/ml sterile solution
- Description: Colorless solution in HEPES buffer
- pH: 7.2 - 7.5
- Purity: ≥ 98.0 %
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PR1MA™ Calcium Chloride Anhydrous
CAS Number: 10043-52-4
Molecular Weight: 110.98
Chemical Formula: CaCl2
Solubility: Water
Storage Temp: Room Temperature
Research or further manufacturing use only, not for food or drug use.
- Appearance: White crystalline powder
- Assay (Titration): ≥ 96.0 %
- Titratable Base: ≤ 0.006 meq/g
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PR1MA™ Rifampicin
CAS Number: 13292-46-1
Molecular Weight: 823.0
Chemical Formula: C43H58N4O12
Solubility: Dilute acid solutions
Storage Temp: 2-8°C
Interferes with synthesis of nucleic acids by inhibiting DNA dependent RNA
polymerase. Active against gram positive bacteria. Less active against gram
negative bacteria. Plant cell culture tested.
Research or further manufacturing use only, not for food or drug use.
- Appearance: Brownish-Red Crystalline Powder
- Crystallinity: Pass Test
- Heavy Metals : ≤ 20 ppm
- Identification: Pass Test
- Loss on Drying: ≤ 1.0 %
- pH: 4.5 - 6.5
- Purity: 97.0 - 102.0 %
- Rifampicine Quinone: ≤ 1.5 %
- Solubility: Pass
- Sulfated Ash: ≤ 0.1 %
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PR1MA™ Hygromycin B Liquid
CAS Number: 31282-04-9Molecular Weight: 527.53
Chemical Formula: C20H37N3O13
Solubility: Water
Storage Temp: 2-8°C
Aminoglycoside antibiotic inhibits growth of prokaryotic (bacteria), eukaryotic microorganism (yeasts) and mammalian cells. Particularly useful as a selective agent for the incorporation of the APH 4 gene in plant tissue. Inhibits peptide chain elongation by preventing elongation-factor EF-2 dependent translocation. Supplied as 1 million units in solution per vial. 1 Million Units of Hygromycin
Research or further manufacturing use only, not for food or drug use.
- Appearance: Light amber solution
- Purity: ≥ 80 %
- Potency: Report U/mg
- Concentration: Report (500 mg Hygromycin B/ml) units/ml
- Density: Report mg / mL
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PR1MA™ PBS [Phosphate Buffered Saline], 10X Powder Concentrate, White Granular Powder
CAS Number: 7778-77-0; 7447-40-7; 7558-79-4; 7647-14-5
Storage Temp: Room Temperature
Research or further manufacturing use only, not for food or drug use.
- Appearance: White Uniform Powder (No Granular)
- Chloride: 0.136 - 0.144 moles/liter
- Conductivity @ 25 oC: 14000 - 17800 µomhos/cm
- DNase, RNase, protease: Not Detected
- pH (10X Solution): 6.7 - 6.9
- pH (1 X Solution): 7.3 - 7.5
- Solubility: Pass Test (9.89%)
- Seal Integrity Testing: Pass Test
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PR1MA™ Sodium Phosphate Dibasic, Anhydrous, ACS Grade
CAS Number: 7558-79-4
Molecular Weight: 142.0
Chemical Formula: Na2HPO4
Solubility: Water
Storage Temp: Room Temperature
Research or further manufacturing use only, not for food or drug use.
- Appearance: White crystals
- Assay: ≥ 99.0 %
- Chloride: ≤ 0.002 %
- Heavy Metals : ≤ 10 ppm
- Insoluble Matter: ≤ 0.01 %
- Iron: ≤ 0.002 %
- Loss on Drying: ≤ 0.2 %
- pH (5% Solution): 8.7 - 9.3
- Sulfate: ≤ 0.005 %
- Yeast and Mold Count: ≤ 100 CFU/g
- Aerobic Plate Count: ≤ 1000 CFU/g
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PR1MA™ Paromomycin Sulfate
CAS Number: 1263-89-4
Molecular Weight: 615.6
Chemical Formula: C23H45N5O14 • H2SO4
Solubility: Water
Storage Temp: Room Temperature
Aminoglycoside antibiotic with mode of action similar to Kanamycin and
Neomycin. Used as a selective agent for the incorporation of the NPT II
(APH3') gene in plant tissue. Plant culture tested.
Research or further manufacturing use only, not for food or drug use.
- Appearance: White to Off White Powder
- Assay: ≥ 675 U/mg
- Identification: Conforms
- Loss on Drying: ≤ 5.0 %
- pH 3.0% Solution @25oC: 5.0 -7.5
- Specific Optical Rotation: +50.0 - +55.0°
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