Molecular Biology Reagents/Chemicals

MIDSCI™ gives you ultra-pure, cost-competitive molecular biology reagents and lab chemicals for life science research; often with significant cost savings over Sigma Aldrich and other lab reagent providers.

PR1MA™ Chemicals can help with a wide variety of research-based requirements.
 
Use our glycerol for polyacrylamide gel electrophoresis. Eliminate Agrobacterium after inoculation with the carbenicillin we carry. Or detect β-Galactosidase activity with X-GAL which, when combined with the IPTG we stock, can be used to detect lac gene expression in cloning applications.
 
We also carry Luria Broth (Lennox), for the propagation and maintenance of E. coli; DTT, in order to maintain –SH groups in a reduced state; and high gel strength agar for tissue culture and microbiological work.

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PR1MA Urea, UltraPure (USP Grade)

CAS Number: 57-13-6
Molecular Weight: 60.06
Chemical Formula: CH4N2O
Solubility: Water
Storage Temp: Room Temperature

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White Crystalline Powder
  • Assay: 99.0 - 100.5 %
  • Identification (A, B): Pass Test
  • Appearance of 20% Solution: Pass Test
  • Melting Point: 132.5 to 134.5 oC
  • Moisture (LOD):  1.0 %
  • Alcohol- Insoluble Matter:  0.04 %
  • Residue on Ignition:  0.1 %
  • Chloride:  5 ppm
  • Sulfate:  0.001 %
  • Alkalinity: To pass test
  • Ammonium:  0.05 %
  • Urea Related Compound A:  0.1 %
  • Individual Unspecified Impurities:  0.1 %
  • Total impurities:  2.0 %
  • Heavy Metals:  0.001 %
  • Iron:  0.001 %
  • Residual Solvents USP 467: Meets requirements
  • BSE/TSE Free: BSE/TSE Free
  • Grade: USP
  • Country of Origin : Report

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PR1MA PBS / Tween 20 10X Solution

CAS Number: 7558-79-4, 7778-77-0, 7647-14-5, 7447-40-7, 9005-64-5, 7732-18-5
Solubility: Water
Storage Temp: Room Temperature

Research or further manufacturing use only, not for food or drug use.

  • Appearance: Clear, yellow solution
  • pH: 7.2 - 7.6

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PR1MA
  Cefotaxime, Sodium Salt

CAS Number: 64485-93-4
Molecular Weight: 477.5
Chemical Formula: C16H16N5NaO7S 2
Solubility: Water
Storage Temp: 2-8°C

Inhibitor of bacterial cell wall synthesis. Inhibits the crosslinking of peptidoglycan by binding and inactivating transpeptidases. High activity against gram negative bacteria. Often used for elimination of Agrobacterium species after inoculation. Resistant to B-Lactamase activity.

Non toxic to plant cells
Plant cell culture tested

Research or further manufacturing use only, not for food or drug use.

  • Appearance: Off White to Pale Yellow Crystalline Powder
  • Assay (Potency dry basis) : 916 - 964 µg/mg
  • Bacterial Endotoxins : Meet the requirements
  • Clarity of Solution: Meet the requirements
  • Color of solution: Meet the requirements
  • Identification (A, B, C): Pass Test
  • Impurity - Individual:  1.0 %
  • Loss on Drying:  3.0 %
  • pH: 4.5 - 6.5
  • Specific Rotation: +58 - +64 °
  • Total impurities:  3.0 %

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PR1MA
  Gentamicin Sulfate

CAS Number: 1405-41-0
Molecular Weight: 575.67
Chemical Formula: C21H43N5O7  H2SO4
Solubility: Water
Storage Temp: Room Temperature

Cell culture tested. Aminoglycoside antibiotic with bactericidal activity against most gram negative bacteria.
 
Research or further manufacturing use only, not for food or drug use.
  • Appearance: White to off white powder
  • Composition (C1): 20.0 - 50.0 %
  • Composition (C1a): 10.0 - 35.0 %
  • Composition (C2a + C2): 25.0 - 55.0 %
  • Identification: Meet The Requirements
  • Loss on Drying:  18.0 %
  • Methanol:  1.0 %
  • pH: 3.5 - 5.5
  • Potency (Dried basis):  590 µg/mg
  • Residual Solvents: Meet Requirements
  • Residue on Ignition:  1.0 %
  • Specific Optical Rotation: +107 - +121 °
  • Country of Origin: Report

Proudly made in the USA! Click here for more made in America products!
 
 


PR1MA Glycine, Free Base

CAS Number: 56-40-6
Molecular Weight: 75.1
Chemical Formula: C2H5NO2
Solubility: Water
Storage Temp: Room Temperature

For use in preparing electrophoresis buffers. Cell culture tested.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White odorless crystalline powder with sweet taste.
  • Solubility (5% in water): Clear & colorless
  • Identification (IR): Test Passes
  • Loss on Drying (@105 oC for 2 hrs):  0.20 %
  • Residue on Ignition:  0.10 %
  • Chloride (Cl):  0.007 %
  • Sulfate:  0.0065 %
  • Heavy Metals:  0.002 %
  • Hydrolyzable Substances: Test Passes
  • Related Compound (Diglycine):  0.1 %
  • Related Compound (Triglycine):  0.1 %
  • Related Compound (Glycine Anhydride):  0.1 %
  • Related Compound (Monochloro Acetic Acid):  0.1 %
  • Related Compound (Iminodiacetic Acid):  0.1 %
  • Related Compound (Haxamathylenetetraamine):  0.1 %
  • Related Compound (Total Impurities):  1.0 %
  • Residual Solvent (Chloroform):  60 mcg/gm
  • Residual Solvent (1,4 Dioxane):  380 mcg/gm
  • Residual Solvent (Methylene Chloride):  600 mcg/gm
  • Residual Solvent (Trichloroethylene):  80 mcg/gm
  • Methanol:  0.3 %
  • Assay: 98.5 - 101.5 %
  • Arsenic:  1.0 ppm
  • Total Viable Bacterial Count:  1000 Cfu/gm
  • Coliform Count: Negative
  • Yeast and Mold Count:  100 CFU/g
  • Test for E-Coli: Absent
  • Test for Salmonella: Absent
  • Test for S-Aureus: Absent
  • Test for Pseudoomonas: Absent
  • BSE/TSE Free: BSE/TSE Free
  • Grade: USP
  • Country of Origin: Report

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[Dextrose Anhydrous]


PR1MA D-(+)-Glucose [Dextrose Anhydrous]

  • Common Name: Dextrose Anhydrous
  • CAS Number: 50-99-7
  • Molecular Weight: 180.16
  • Chemical Formula: C6H12O6
  • Solubility: Water
  • Storage Temp: Room Temperature

USP Grade material.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White Crystalline Powder
  • Assay: 97.5 - 102.0 %
  • Identification (FTIR Spectrum): Pass Test
  • Identification (HPLC): Matches the reference
  • Specific Rotation: +52.6 - +53.2 °
  • Moisture:  1.0 %
  • Residue on Ignition:  0.1 %
  • Color of solution: Qualified
  • Conductivity:  20.0 µs/cm
  • Dextrins: Qualified
  • Soluble starch and sulfite: Qualified
  • Related Substance ( Maltitol and Isomalt):  0.4 %
  • Related Substance (Maltotriose):  0.2 %
  • Related Substance (Fructose):  0.15 %
  • Related Substance ( Unspecified Impurities):  0.1 %
  • Related Substance (Total impurity):  0.5 %
  • Heavy Metals:  5 ppm
  • Residual Solvents: None
  • Total Bacterial Count:  1000 cfu/g
  • Mold and Yeast:  100 cfu/g
  • E.Coli: Negative
  • Endotoxin:  1.25 EU/g
  • Source: Corn
  • Grade: Meets the current USP Grade
  • BSE/TSE Free: BSE/TSE Free
  • Country of Origin: Report

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PR1MA
  Carbenicillin, Disodium Salt

CAS Number: 4800-94-6
Molecular Weight: 422.4
Chemical Formula: C17H16N2Na2O 6S
Solubility: Water
Storage Temp: 2-8°C

USP grade

Prevents bacterial cell wall synthesis. Inhibits crosslinking of peptidoglycan by binding and inactivating transpeptidases. Highly active against gram negative bacteria. Commonly used for the elimination of Agrobacterium species after inoculation. Sensitive to B-Lactamase.

  • Non-toxic to plant cells
  • Plant cell culture tested
  • Hygroscopic

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White to Off White Crystalline Powder
  • Identification, Sodium Reaction: Pass Test
  • Water:  6.0 %
  • pH: 6.50 - 8.0
  • Clarity of Solution: Clear Solution
  • Benzyl penicillin sodium:  5.0 %
  • Chromatographic Purity (HPLC):  92.0 %
  • Assay (Anhydrous):  88.0 %
  • Bacterial Endotoxins :  0.05 EU/mg
  • Iodine Absorbing Substances:  8.0 %
  • Potency (Dried basis):  770 µg/mg
  • Grade: USP
  • Country of Origin : Report

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PR1MA bis-Acrylamide

CAS Number: 110-26-9
Molecular Weight: 154.2
Chemical Formula: C7H10N2O2
Solubility: Water
Storage Temp: Room Temperature

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White Crystalline Powder
  • Absorbance 290 nm (1% water ):  0.2
  • Acrylic Acid:  0.001 %
  • Conductivity (2% Water):  5 umhos
  • DNase: Not Detected
  • Identification: Pass
  • pH (1%, 0.1M NaCl) 25oC:  5.0
  • Protease: Not Detected
  • Purity:  99.0 %
  • RNase: Not Detected

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PR1MA  Kanamycin Monosulfate [Kanamycin A]

CAS Number: 25389-94-0
Molecular Weight: 582.6
Chemical Formula: C18H36N4O11  H2SO4
Solubility: Water
Storage Temp: Room Temperature

Aminoglycoside antibiotic with a bactericidal action against most gram negative bacteria. Incorporates into sensitive bacterial cells by an active transport process. Binds to the 30S and to some extent the 50S subunits of the bacterial ribosome, inhibiting protein synthesis and generating errors in the transcription of the genetic code. Widely used as a selective agent for the incorporation of the NPT II (APH3') gene in plant tissue.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White Powder
  • Chromatographic Purity: Pass Test
  • Identification: Pass Test
  • Loss on Drying:  1.5 %
  • pH (1% Solution): 6.5 - 8.5
  • Potency:  750 µg/mg
  • Residue on Ignition:  1.0 %
  • Specific Rotation: +116 - +123°

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PR1MA Bicine Ultra-Pure

CAS Number: 150-25-4
Molecular Weight: 163.2
Chemical Formula: C6H13NO4
Solubility: Water
Storage Temp: Room Temperature

Zwitterionic buffer used in a variety of molecular biology applications such as protein resolution, protein crystallization and SDS-PAGE.

pKa @ 25°C: 8.3
pH Range: 7.6-9.0
Melting Range: 190-192°C

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White Powder
  • FTIR: Conforms to reference
  • Optical ABS @ 260 NM:  0.1
  • Optical ABS @ 280 NM:  0.1

 

Proudly made in the USA! Click here for more made in America products!
 
 


PR1MA PBS [Phosphate Buffered Saline]

 

Common Name: Phosphate Buffered Saline
CAS Number: 7647-14-5; 7447-40-7; 7558-79-4; 7778-77-0
Solubility: Water
Storage Temp: Room Temperature

 

PBS, 100 mL Tablets refer to pre-weighed tablets that are used to prepare a specific volume of PBS (Phosphate Buffered Saline) solution. 

PBS is a widely used buffer solution in biological and biochemical research. PBS serves as a versatile buffer for various applications, including cell culture, immunohistochemistry, protein and nucleic acid research, and diagnostic assays. It helps maintain a stable pH and osmolarity, providing optimal conditions for biological reactions and preserving the integrity of cells and biomolecules. 

Each tablet contains pre-determined amounts of sodium chloride, sodium phosphate dibasic, and potassium phosphate monobasic. When dissolved in distilled water or a suitable solvent, the tablet dissolves, releasing the components, and instantly forms a ready-to-use PBS solution with a total volume of 100 mL.

These tablets ensure accurate and consistent preparation of PBS, eliminating the need for weighing and measuring individual components. They are designed to simplify the process and save time in laboratory workflows.

  

Research or further manufacturing use only, not for food or drug use.

 

Appearance: White, Concave Tablets

Clarity and Color of Solution: Clear colorless solution (No Cloudy) 5 tablets in 500 ml H2O

Hardness Test: Non-Fragile, No Chips, No Broken Tablets

pH (1 Tablet/ 100 ml Water): 7.3 - 7.5

 

Proudly made in the USA! Click here for more made in America products!

[1,3-Diaza-2-,4-cyclopentadiene]

PR1MA Imidazole [Glyoxaline] [1,3-Diaza-2-,4-cyclopentadiene]

Common Name: [Glyoxaline] [1,3-Diaza-2,4-cyclopentadiene]
CAS Number: 288-32-4
Molecular Weight: 68.1
Chemical Formula: C3H4N2
Solubility: Water
Storage Temp: Room Temperature

pKa (25°C): 6.9-7.0
pH range: 6.2-7.8

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White to off white crystals
  • Assay:  99.0 %
  • DNase: Not Detected
  • Identification: Pass Test
  • Loss on Drying:  0.5 %
  • Melting Point: 88 - 92 oC
  • OPTICAL @ 260 NM:  0.2
  • PKa @ 25°C: 6.79 - 7.19
  • Protease: Not Detected
  • RNase: Not Detected

Proudly made in the USA! Click here for more made in America products!
 
 

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