PR1MA™ Yeast Extract, Powder

CAS Number: 8013-01-2
Solubility: Water
Storage Temp: Room Temperature

Dried yeast autolysate with a high content of amino nitrogen and water soluble B complex vitamins. Due to its low NaCl content this quality is well suited for tissue culture.

Ingredients:

• Total Nitrogen (TN): ~9.8%
• Amino Nitrogen (AN): ~5.1%
• AN/TN Ratio: ~0.52
• Sodium Chloride: ~0.3%
• pH (1% Solution): ~7.0

• Appearance: Light Beige Powder
• Amino Nitrogen: 4.5 - 5.8 %
• Ash Content: 11.50 - 16.0 %
• Dry Matter: ≥ 95.0 %
• Loss on Drying: ≤ 5.0 %
• pH (2.0% Solution @ 25 ^oC): 6.8 - 7.2
• Sodium Chloride: ≤ 0.5 %
• Total Nitrogen: 10.0 - 11.8 %
• Clostridium Perfringens Spores : Report cfu/g
• E.Coli: Report cfu/g
• Molds and Yeasts: Report cfu/g
• Pathenogenic Staphylococcus: Report cfu/g
• Salmonella : Report cfu/25g
• Standard Plate Count: Report cfu/g

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PR1MA™ 2XYT Broth, Capsules

CAS Number: 91079-40-2; 8013-01-2; 7647-14-5
Solubility: Water
Storage Temp: Room Temperature

Nutritionally enriched medium for the growth of recombinant strains of E. Coli and in the preparation and transformation of competent cells.

2XYT Broth packaged in convenient ready to use capsules. Each capsule contains 15.5 g 2XYT Broth.

Simply add 2 capsules to one liter distilled water and heat until completely dissolved. Sterilize by autoclaving at 121°C for 15 minutes. Final pH at 25°C: 6.8-7.2.

Ingredients:

• Digest Peptone: 16 g/l
• Yeast Extract: 10 g/l
• Sodium Chloride:5 g/l

Research or further manufacturing use only, not for food or drug use.

• Appearance: Capsule (Cream to beige free flowing powder)
• DCM Appearance: Capsule (Homogeneous, free flowing, beige)
• PPM Appearance: Capsule (Clear, no to light ppt, yellow)
• pH (3.1 % Solution @25 ^oC): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent
• Casein Peptone: 16.0 g/L
• Yeast Extract: 10.0 g/L
• Sodium Chloride: 5.0 g/l
• Description: Add 2 capsules 2XYT broth in 1 liter purified water until evenly dispersed. Boil for 1 minute with stirring to completely dissolve. Distribute and autoclave at 121^oC for 15 minutes.

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PR1MA™ 2XYT Broth, Powder

CAS Number: 91079-40-2; 8013-01-2; 7647-14-5
Solubility: Water
Storage Temp: Room Temperature

Nutritionally enriched medium for the growth of recombinant strains of E. Coli and in the preparation and transformation of competent cells.

Dissolve 31g in one liter distilled water. Heat solution to boiling with continuous stirring. Sterilize by autoclaving at 121°C for 15 minutes. Final ph @ 25°C: 6.8-7.2.

Ingredients:

• Digest Peptone: 16 g/l
• Yeast Extract: 10 g/l
• Sodium Chloride: 5 g/l

Research or further manufacturing use only, not for food or drug use.

• Appearance: Cream to beige free flowing powder
• DCM Appearance: Homogeneous free flowing, beige
• PPM Appearance: Clear,no to light ppt,yellow
• pH (3.1 % Solution @25 ^oC): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

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PR1MA™ Luria Broth Granulated [Miller's LB Broth]

Common Name: Miller's LB Broth
CAS Number: 91079-40-2; 8013-01-2; 7647-14-5
Solubility: Water
Storage Temp: Room Temperature

For propagation and maintenance of E. Coli. High Salt Formula. Molecular Biology Grade.

Dissolve 25 grams in 1 liter distilled water and adjust the pH to 7.2. Heat and frequently agitate to completely dissolve. Sterilize by autoclaving for 15 minutes. Material specially granulated to reduce dust and increase solubility rate.

Ingredients:

• Tryptone:10 g/L
• Sodium Chloride: 10 g/L
• Yeast Extract: 5 g/L

Research or further manufacturing use only, not for food or drug use.

• Appearance: Cream to beige granular powder
• DCM Appearance: Homogeneous, free flowing beige to yellow-beige
• PPM Appearance: Clear, yellow to amber no precipitate (ppt)
• Appearance of Solution: Prepared medium is yellow to gold and clear to moderately hazy
• pH (2.5% H₂O @25 °C): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent
• Casein Peptone: 10 g/L
• Sodium Chloride: 10 g/L
• Yeast Extract: 5 g/L

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PR1MA™ Peptone, Powder

CAS Number: 73049-73-7
Solubility: Water
Storage Temp: Room Temperature

Mixture of peptides and free amino acids from pancreatic hydrolysis of animal tissue. Low NaCl content suitable for tissue culture.

Total Nitrogen (TN): 15.0-16.0%
Amino Nitrogen (AN): 3.0-3.5%
AN/TN Ratio: 0.21-0.23

Research or further manufacturing use only, not for food or drug use.

• Appearance: White to Light Beige Fine Powder
• Amino Nitrogen: ≥ 3.4 %
• Ash, Residue in Ignition: ≤ 15 %
• Loss on Drying: ≤ 5 %
• Nitrites: Negative
• pH (2.0% Solution @ 25 ^oC): 6.5 - 7.5
• Proteoses: Report
• Total Nitrogen: ≥ 10 %
• Undigested Protein: Negative
• Coliforms: Negative
• E.Coli: Negative
• Molds and Yeasts: Report cfu/g
• Salmonella : Negative cfu/25g
• Standard Plate Count : ≤ 1000 cfu/g
• Broth Formulations (Growth Supporting Properties): Satisfactory
• Peptone Agar (Growth Supporting Properties): Satisfactory
• Raw Material Species: Bovine
• Enzyme Species: Porcine

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PR1MA™ Yeast Extract, Granulated

CAS Number: 8013-01-2
Solubility: Water
Storage Temp: Room Temperature

Dried yeast autolysate with a high content of amino nitrogen and water soluble B complex vitamins. Material is specially granulated to reduce dust and increase solubility rate.

Suitable for Microbiology Applications.

Research or further manufacturing use only, not for food or drug use.

• Appearance: Light Beige Powder
• Amino Nitrogen Content: ≥ 3.5 %
• Dry Matter: ≥ 94 %
• Moisture : ≤ 6.0 %
• pH (2.0% Solution @ 25 ^oC): 6.5 - 7.5
• Sodium Chloride: ≤ 0.5 %
• Total Nitrogen Content: ≥ 8.0 %
• Non Animal Origin: The product does not come in contact with materials of animal origin.

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PR1MA™ Luria Agar Powder [Miller's LB Agar]

Common Name: Miller's LB Agar
CAS Number: 91079-40-2; 8013-01-2; 7647-14-5; 9002-18-0
Solubility: Water with Heat
Storage Temperature: Room Temperature

For propagation and maintenance of E. Coli. High Salt Formula. Molecular Biology Grade.

• Dissolve 40 grams in 1 liter distilled water
• Adjust pH to 7.2
• Sterilize by autoclaving for 15 minutes

Ingredients:

• Peptone: 10g/L
• Yeast: 5 g/L
• Sodium Chloride: 10g/L
• Agar: 15g/L

Research or further manufacturing use only, not for food or drug use.

• DCM Appearance: Homogeneous Free Flowing, Light Beige
• PPM Appearance: Clear, no to light ppt, light yellow
• pH (4% solution at 25°C): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

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PR1MA™ 2XYT Broth, Granulated

CAS Number: 91079-40-2; 8013-01-2; 7647-14-5
Solubility: Water
Storage Temp: Room Temperature

Nutritionally enriched medium for the growth of recombinant strains of E. Coli and in the preparation and transformation of competent cells.

Material is specially granulated to reduce dust and increase solubility rate.

Dissolve 31g in one liter distilled water. Heat solution to boiling with continuous stirring. Sterilize by autoclaving at 121°C for 15 minutes. Final pH@ 25°C: 6.8-7.2.

Ingredients:

• Digest Peptone: 16 g/l
• Yeast Extract: 10 g/l
• Sodium Chloride: 5 g/l

Research or further manufacturing use only, not for food or drug use.

• Appearance: Cream to beige free flowing granulated
• DCM Appearance: Homogeneous free flowing, beige
• PPM Appearance: Clear,no to light ppt,yellow
• pH (3.1 % Solution @25 ^oC): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

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PR1MA™ Tryptone, Powder

CAS Number: 91079-40-2
Solubility: Water
Storage Temp: Room Temperature

Pancreatic digest of casein used to supplement media in cultivation of anaerobic bacteria.

pH (1% solution): 7.3.

Ingredients:

• Molecular Biology GradeTotal Nitrogen (TN): ~12.7%
• Amino Nitrogen (AN): ~3.7%
• AN/TN Ratio: 0.26
• Sodium Chloride: ~0.4%
• Tryptophan: ~1.0

Research or further manufacturing use only, not for food or drug use.

• Appearance: Cream to beige free flowing powder
• Amino Nitrogen: ≥ 4.0 %
• Ash: ≤ 15 %
• Loss on Drying: ≤ 5.0 %
• Nitrites: Negative
• pH (2% water) @25°C: 6.5 - 7.5
• Total Nitrogen: ≥ 10 %
• Undigested Protein: Negative
• Broth Formulations (Growth Supporting Properties): Satisfactory
• Peptone Agar (Growth Supporting Properties): Satisfactory
• Proteoses: Positive
• Salmonella: Negative/25g
• Standard Plate Count : ≤ 1000 cfu/g
• Coliform: Negative
• E.Coli: Negative
• Molds and Yeasts: ≤ 100 cfu/g
• Raw Material Species: Bovine
• Enzyme Species: Porcine
• BSE/TSE Free: BSE/TSE Free
• Country of Origin : Report

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PR1MA™ Peptone, Granulated

CAS Number: 73049-73-7
Solubility: Water
Storage Temp: Room Temperature

Mixtures of peptides and free amino acids from peptic digest of animal tissue.

• Amino Nitrogen (AN): 2.5-3.5%
• Loss on Drying: <6.0%

Research or further manufacturing use only, not for food or drug use.

• Appearance: Light Beige Granular Powder
• Amino Nitrogen Content: ≥ 3.4 %
• Appearance of Solution: Clear and yellow-beige
• Ash: ≤ 15.0 %
• Loss on Drying: ≤ 5.0 %
• pH (2.0% Solution @ 25 ^oC): 6.5 - 7.5
• Total Nitrogen Content: ≥ 10.0 %

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PR1MA™ qMAX Green qPCR Mix with Blue Tracking Dye

• Superior sensitivity and fast cycling with exceptional results
• Ideal for low copy number templates
• Early Ct values and detection across a broad dynamic range
• Ready to use 2X Master Mix
• Includes PR1MA™ Hot Start Taq Polymerase for greater specificity and accuracy 

Supplied as a ready-to-use 2X master mix, PR1MA™ qMax Green has been engineered for high sensitivity, fast cycling, and excellent reproducibility. PR1MA™ Hot Start Polymerase provides accurate PCR of a variety of templates including low copy number and difficult sequences, while the proprietary qMax Green intercalating dye exhibits higher fluorescence and lower PCR inhibition than other popular green dyes.

These two components are supported by a specially formulated buffer with an exacting combination of salts, PCR enhancers, stabilizers, and pH that results in earlier Ct values and a high specificity across a broad dynamic range.

*Please note these products ship on dry ice. Appropriate shipping charges apply unless otherwise noted on a quote.

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PR1MA™ Sodium Chloride, Powder

CAS Number: 7647-14-5
Molecular Weight: 58.4
Chemical Formula: NaCl
Solubility: Water
Storage Temp: Room Temperature

Research or further manufacturing use only, not for food or drug use.

• Appearance: Fine White Granular Powder
• Acidity or Alkalinity: Pass Test
• Appearance of Solution: Clear, Colorless Solution
• Arsenic : ≤ 1 µg/g
• Assay: 99.0 - 100.5 %
• Barium: Pass Test
• Bromides: ≤ 0.010 %
• Ferrocyanide: Pass Test
• Heavy Metals : ≤ 5 ppm
• Identification (Chloride): Pass Test
• Identification (Sodium): Pass Test
• Loss on Drying: ≤ 0.5 %
• Magnesium & Alkaline Earth Metals:  0.01 %
• Iron(Fe): ≤ 2 µg/g
• Nitrites: ≤ 0.01
• Phosphates : ≤ 0.0025 %
• Sulfate: ≤ 0.020 %

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