Molecular Biology Reagents/Chemicals

MIDSCI™ gives you ultra-pure, cost-competitive molecular biology reagents and lab chemicals for life science research; often with significant cost savings over Sigma Aldrich and other lab reagent providers.

PR1MA™ Chemicals can help with a wide variety of research-based requirements.
 
Use our glycerol for polyacrylamide gel electrophoresis. Eliminate Agrobacterium after inoculation with the carbenicillin we carry. Or detect β-Galactosidase activity with X-GAL which, when combined with the IPTG we stock, can be used to detect lac gene expression in cloning applications.
 
We also carry Luria Broth (Lennox), for the propagation and maintenance of E. coli; DTT, in order to maintain –SH groups in a reduced state; and high gel strength agar for tissue culture and microbiological work.

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PR1MA
 Agarose SS, Super High Strength, Molecular Biology Grade

CAS Number: 9012-36-6
Solubility: Water
Storage Temp: Room Temperature

For DNA/RNA fragments >1000 bp. Ideal for separating larger molecules. DNAse/RNase free.

Gel Strength, 1%: >1800 g/cm2
Gel Strength, 1.5%: >3200 g/cm2
Gelling Temperature: 35-37°C
Melting Temperature: 87-89°C
Sulfate: <0.12%
EEO: <0.13%

Research or further manufacturing use only, not for food or drug use.

  • Appearance: Fine white homogeneous powder
  • DNase/ RNase: Not Detected
  • Gelling Temperature:  10 oC
  • Melting Point: 80 - 100 oC

Proudly made in the USA! Click here for more made in America products!
 
 

Item#:
ASPRIMAAGASHS
[Benzyl Penicillin Sodium Salt]

PR1MA Penicillin G Sodium Salt [Benzyl Penicillin Sodium Salt]

Common Name: Benzyl Penicillin Sodium Salt
CAS Number: 69-57-8
Molecular Weight: 356.4
Chemical Formula: C16H17N2NaO4S
Solubility: Water
Storage Temp: 2-8°C

Inhibitor of bacterial cell wall synthesis. Active against gram positive and some gram negative bacteria. β-Lactamase sensitive.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White crystalline powder
  • Potency : 1500 - 1750 U/mg
  • Assay: 96.0 - 102.0 %
  • Identification: Pass Test
  • Loss on Drying:  1.0 %
  • pH: 5.5 - 7.5
  • Specific Optical Rotation: +285 - +310 °
  • Absorbance 264 nm: 0.80 - 0.88
  • Absorbance 280nm:  0.10
  • Absorbance 325nm:  0.10
  • Related Substances: Conforms to standard
  • Bacterial Endotoxins : Conforms to standard
  • Sterility: Conforms to standard
  • Country of Origin : Report

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PR1MA Novobiocin Sodium Salt

CAS Number: 1476-53-5

Molecular Weight: 634.61

Chemical Formula: C31H35N2NaO11

Solubility: Water

Storage Temp: 2-8°C

 

Novobiocin Sodium Salt is an antibiotic that is a member of the coumarin class of antibiotics and is mainly effective against Gram-positive bacteria. In microbiology and molecular biology research, Novobiocin Sodium Salt is used as a selective agent.

Research or further manufacturing use only, not for food or drug use. 

  • Appearance: White to yellow powder
  • Description - USP: Meets Test
  • Novobiocin (Dried Basis)-USP: ≥ 850 µg/mg
  • Identification - USP: Positive
  • Specific Rotation Dried Basis - USP: -58 to -50  o
  • Identification A - USP: Positive
  • Identification B - USP: Positive
  • pH(USP): 6.5 - 8.5
  • Loss on Drying - USP: ≤ 6.0 %
  • Residue on Ignition - USP: 10.5 - 12.0 %
  • Crystallinity - USP: Meets Test
  • Acetone - USP: ≤ 5000 ppm
  • Isooctane - USP: ≤ 5000 ppm
  • Assay Use Value: ≥ 845 ug/mg
  • Impurities Total HPLC Normal: ≤ 10.0 %
  • Impurities Unspecified: ≤ 0.2 %
  • Novobiocin-2,3-Carbonate: ≤ 0.6 %
  • A-Ring Amide: ≤ 0.3 %
  • Decarbamyl Novobiocin: ≤ 5.0 %
  • Isonovobiocin: ≤ 3.0 %
  • A-Ring Hydroxy: ≤ 1.0 %
  • Carbamyl Novobiocin: ≤ 3.0 %
  • DES-2-Isobutenyl: ≤ 0.5 %
  • DES-A-Ring Alpha-Keto-Carboxylic Acid: ≤ 0.5 %
  • O-Desmethylnovobiocin: ≤ 0.3 %
  • O-Desmethyl descarbamyl novobiocin: ≤ 0.3 %
  • A-Ring Carbamyl: ≤ 0.5 %
  • 8- Desmethyl novobiocin: ≤ 0.2 %
  • Carbamyl Novobiocin + Decarbamyl Novobiocin + Isonovobiocin: ≤ 11.0 %
  • BSE/TSE Free: BSE/TSE Free
  • Country of Origin: USA 

Proudly made in the USA! Click here for more made in America products!
 
 

PR1MA Proteinase K

CAS Number: 39450-01-6
Solubility: Water
Storage Temp: -20°C

From Tritirachium album. Serine protease for proteolytic inactivation of nucleases during isolation of DNA and RNA.

Shipping Weight: 0 lbs
Shipping Dimensions: 1.25 x 1.75 x 1.25


Specifications

Appearance: White to Off White Lyophilized Solid
Solubility: 50mM Tris-HCl (pH 7.5), 3mM CaCl2, 50% Glycerol
Purity: ≥ 98.0 %
DNase/ RNase: Not Detected
Activity: ≥ 30.0 U/mg
Unit Definition: 1 unit is defined as the amount of enzyme that will liberate 1.0 µmol of tyrosine from casein/min @37°C, pH 7.5
Country of Origin: China

Research or further manufacturing use only, not for food or drug use.

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PR1MA Formamide, Super Pure

CAS Number: 75-12-7
Molecular Weight: 45.04
Chemical Formula: CH3NO
Storage Temperature: 2-8°C

For nucleic acid hybridization and sequencing

Shipping Weight: 0.1 lbs
Shipping Dimensions: 3.38 x 4.63 x 3.38

Specifications
  • Appearance: Clorless Liquid
  • Assay: ≥ 99.5 %
  • Freezing Point: 1.0 - 3.0 °C
  • Copper: ≤ 0.0001 %
  • Iron: ≤ 0.0005 %
  • Lead (Pb): ≤ 0.0001 %
  • Zinc: ≤ 0.0001 %
Research or further manufacturing use only, not for food or drug use.

Proudly made in the USA! Click here for more made in America products!
 
 

PR1MA Sucrose

CAS Number: 57-50-1
Molecular Weight: 342.3
Chemical Formula: C12H22O11
Storage Temp: Room Temperature

Ultra-high purity, double crystallized to remove any remaining inorganic and organic contamination. Produces a clear colorless solution. Used for cultivating protoplasts and "in vitro" multiplication of plant tissue.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: Fine White Crystals
  • Absorbance 260nm:  0.07 nm
  • Absorbance 280nm:  0.05
  • DNase, RNase, protease: Not Detected
  • Heavy Metals:  0.0005 %
  • Identification (IR): Pass
  • Purity:  99.0 %
  • Solubility (10% solution in H2O @ 25oC): Pass
  • Sulfate:  0.005 %

Proudly made in the USA! Click here for more made in America products!
 
 

PR1MA Sodium Acetate, Anhydrous

CAS Number: 127-09-3
Molecular Weight: 82.0
Chemical Formula: C2H3O2Na
Solubility: Water
Storage Temp: Room Temperature

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White Powder
  • Assay: ≥ 99.0 %
  • pH (5% Solution): 7.0 - 9.2
  • Solubility: Soluble in Water
  • Loss on Drying (120 oC):  1.0 %
  • Calcium (Ca):  0.005 %
  • Chloride (Cl):  0.002 %
  • Heavy Metals (as Pb):  0.001 %
  • Insoluble Matter:  0.01 %
  • Iron (Fe):  0.001 %
  • Magnesium (Mg):  0.002 %
  • Phosphate:  0.001 %
  • Sulfate (SO4):  0.003 %
  • Grade: Meet USP/ACS Specifications

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PR1MA Phleomycin 20 mg / mL Solution

CAS Number: 11006-33-0
Molecular Weight: 1526.5 g/mol
Chemical Formula: C55H85O21N20S 2Cu  HCl
Solubility: Water
Storage Temp: -20°C

Supplied at a concentration of 20 mg/ml.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: Clear Blue Solution
  • Concentration: = 20 mg/ml
  • Odor: None
  • Solubility (20 mg/ml in Water): Freely soluble
  • Sterile Filtered: Pass test
  • UV Absorbance 244nm (200 ug/ml): 0.75 - 1.05

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PR1MA  Thiamine Hydrochloride [Vitamin B1]

Common Name: Vitamin B1
CAS Number: 67-03-8
Molecular Weight: 337.3
Chemical Formula: C12H17CIN4OS  HCl
Solubility: Water
Storage Temp: 2-8°C

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White Crystalline Powder
  • Appearance of Solution: Clear, no more color than reference solution Y7 or GY7
  • Identification: Should be positive reaction
  • Identification (IR): Consistent with the reference IR spectra
  • pH: 2.7 - 3.3
  • Sulfates:  300 ppm
  • Limit of Nitrate: No brown ring is produced at the junction of the two layers
  • Lead:  2 ppm
  • Water:  5.0 %
  • Residue on Ignition:  0.1 %
  • Absorbance of Solution:  0.025
  • Residual Solvents (Methanol):  0.3 %
  • Residual Solvents (Ethanol):  0.5 %
  • Related Substance (Impurity A):  0.15 %
  • Related Substance (Impurity B):  0.3 %
  • Related Substance (Impurity C):  0.15 %
  • Related Substance (Any Other Impurity):  0.1 %
  • Related Substance (Total impurities):  0.5 %
  • Related Compounds:  1.0 %
  • Chromatographic Purity:  1.0 %
  • Assay: 98.5 - 101.0 %
  • Staphylococcus Aureus: Negative
  • Salmonella: Negative
  • E.Coli: Negative
  • Total Plate Count:  1000 cfu/g
  • Yeast and Mold:  100 cfu/g
  • Material Source: Acrylonitrate; Acetamidine HCl; Chlorine Ester
  • Production Method: Chemical Synthesis
  • BSE/TSE Free: BSE/TSE Free
  • Non Animal Origin: Non Animal Origin
  • Conclusion: This product complies with the specification of BP/USP/FCC.
  • Country of Origin: Report

Proudly made in the USA! Click here for more made in America products!
 
 

PR1MA TBE Buffer 10x Powder

Common Name: TRIS-Borate-EDTA
CAS Number: 77-86-1; 10043-35-3;6381-92-6/60-00-4
Solubility: Water
Storage Temp: Room Temperature

Dry homogeneous blend of molecular biology grade TRIS base, Boric Acid and EDTA.

For use in gel electrophoresis.

Shipping Weight: 0.5 lbs
Shipping Dimensions: 3.50 x 3.75 x 3.50
Appearance: White Powder
pH (1 X Solution): 8.20 - 8.40
Solubility: Clear and Colorless

Research or further manufacturing use only, not for food or drug use.
 

Proudly made in the USA! Click here for more made in America products!
 
 

[N-(2-Hydroxyethyl) piperazine-N'-(2-ethanesulfonic acid)]

PR1MA  HEPES, Sodium Salt [N-(2-Hydroxyethyl) piperazine-N'-(2-ethanesulfonic acid)]

Common Name: N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)
CAS Number: 75277-39-3
Molecular Weight: 260.3
Chemical Formula: C8H17N2O4SNa
Solubility: Water
Storage Temp: Room Temperature

  • pKa (20°C): 7.45-7.65
  • pH Range: 6.8-8.2

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White crystalline powder
  • Assay (Titration, dried basis):  98.0 %
  • Chloride (Cl):  0.1 %
  • Heavy Metals (as Pb):  1 ppm
  • Loss on Drying @ 105 oC, 3hrs:  2.0 %
  • pH (1% aqueous @ 25 oC): 9.4 - 11.0
  • pKa @ 20°C: 7.45 - 7.65
  • Solubility (1M Solution in H2O): Clear, complete and colorless
  • UV Absorbance @ 250nm 1M:  1.0

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PR1MA Hygromycin B Powder  

CAS Number: 31282-04-9
Molecular Weight: 527.5
Chemical Formula: C20H37N3O13
Solubility: Water
Storage Temp: 2-8°C

Aminoglycoside antibiotic inhibits protein synthesis in prokaryote and eukaryote cells. Allows selection of cells expressing the Hygromycin B resistant gene. Working concentrations vary from 30-1000 ug/mg, depending on cell lines and growth conditions.

Shipping Weight: 0 lbs

Shipping Dimensions: 1.25 x 1.75 x 1.25

Research or further manufacturing use only, not for food or drug use.
 

Proudly made in the USA! Click here for more made in America products!
 
 

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