Molecular Biology Reagents/Chemicals

MIDSCI™ gives you ultra-pure, cost-competitive molecular biology reagents and lab chemicals for life science research; often with significant cost savings over Sigma Aldrich and other lab reagent providers.

PR1MA™ Chemicals can help with a wide variety of research-based requirements.
 
Use our glycerol for polyacrylamide gel electrophoresis. Eliminate Agrobacterium after inoculation with the carbenicillin we carry. Or detect β-Galactosidase activity with X-GAL which, when combined with the IPTG we stock, can be used to detect lac gene expression in cloning applications.
 
We also carry Luria Broth (Lennox), for the propagation and maintenance of E. coli; DTT, in order to maintain –SH groups in a reduced state; and high gel strength agar for tissue culture and microbiological work.

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PR1MA
  Cefotaxime, Sodium Salt

CAS Number: 64485-93-4
Molecular Weight: 477.5
Chemical Formula: C16H16N5NaO7S 2
Solubility: Water
Storage Temp: 2-8°C

Inhibitor of bacterial cell wall synthesis. Inhibits the crosslinking of peptidoglycan by binding and inactivating transpeptidases. High activity against gram negative bacteria. Often used for elimination of Agrobacterium species after inoculation. Resistant to B-Lactamase activity.

Non toxic to plant cells
Plant cell culture tested

Research or further manufacturing use only, not for food or drug use.

  • Appearance: Off White to Pale Yellow Crystalline Powder
  • Assay (Potency dry basis) : 916 - 964 µg/mg
  • Bacterial Endotoxins : Meet the requirements
  • Clarity of Solution: Meet the requirements
  • Color of solution: Meet the requirements
  • Identification (A, B, C): Pass Test
  • Impurity - Individual:  1.0 %
  • Loss on Drying:  3.0 %
  • pH: 4.5 - 6.5
  • Specific Rotation: +58 - +64 °
  • Total impurities:  3.0 %

Proudly made in the USA! Click here for more made in America products!
 
 


PR1MA
  Gentamicin Sulfate

CAS Number: 1405-41-0
Molecular Weight: 575.67
Chemical Formula: C21H43N5O7  H2SO4
Solubility: Water
Storage Temp: Room Temperature

Cell culture tested. Aminoglycoside antibiotic with bactericidal activity against most gram negative bacteria.
 
Research or further manufacturing use only, not for food or drug use.
  • Appearance: White to off white powder
  • Composition (C1): 20.0 - 50.0 %
  • Composition (C1a): 10.0 - 35.0 %
  • Composition (C2a + C2): 25.0 - 55.0 %
  • Identification: Meet The Requirements
  • Loss on Drying:  18.0 %
  • Methanol:  1.0 %
  • pH: 3.5 - 5.5
  • Potency (Dried basis):  590 µg/mg
  • Residual Solvents: Meet Requirements
  • Residue on Ignition:  1.0 %
  • Specific Optical Rotation: +107 - +121 °
  • Country of Origin: Report

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PR1MA
  Carbenicillin, Disodium Salt

CAS Number: 4800-94-6
Molecular Weight: 422.4
Chemical Formula: C17H16N2Na2O 6S
Solubility: Water
Storage Temp: 2-8°C

USP grade

Prevents bacterial cell wall synthesis. Inhibits crosslinking of peptidoglycan by binding and inactivating transpeptidases. Highly active against gram negative bacteria. Commonly used for the elimination of Agrobacterium species after inoculation. Sensitive to B-Lactamase.

  • Non-toxic to plant cells
  • Plant cell culture tested
  • Hygroscopic

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White to Off White Crystalline Powder
  • Identification, Sodium Reaction: Pass Test
  • Water:  6.0 %
  • pH: 6.50 - 8.0
  • Clarity of Solution: Clear Solution
  • Benzyl penicillin sodium:  5.0 %
  • Chromatographic Purity (HPLC):  92.0 %
  • Assay (Anhydrous):  88.0 %
  • Bacterial Endotoxins :  0.05 EU/mg
  • Iodine Absorbing Substances:  8.0 %
  • Potency (Dried basis):  770 µg/mg
  • Grade: USP
  • Country of Origin : Report

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PR1MA  Kanamycin Monosulfate [Kanamycin A]

CAS Number: 25389-94-0
Molecular Weight: 582.6
Chemical Formula: C18H36N4O11  H2SO4
Solubility: Water
Storage Temp: Room Temperature

Aminoglycoside antibiotic with a bactericidal action against most gram negative bacteria. Incorporates into sensitive bacterial cells by an active transport process. Binds to the 30S and to some extent the 50S subunits of the bacterial ribosome, inhibiting protein synthesis and generating errors in the transcription of the genetic code. Widely used as a selective agent for the incorporation of the NPT II (APH3') gene in plant tissue.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White Powder
  • Chromatographic Purity: Pass Test
  • Identification: Pass Test
  • Loss on Drying:  1.5 %
  • pH (1% Solution): 6.5 - 8.5
  • Potency:  750 µg/mg
  • Residue on Ignition:  1.0 %
  • Specific Rotation: +116 - +123°

Proudly made in the USA! Click here for more made in America products!
 
 

 

PR1MA Bicine Ultra-Pure

CAS Number: 150-25-4
Molecular Weight: 163.2
Chemical Formula: C6H13NO4
Solubility: Water
Storage Temp: Room Temperature

Zwitterionic buffer used in a variety of molecular biology applications such as protein resolution, protein crystallization and SDS-PAGE.

pKa @ 25°C: 8.3
pH Range: 7.6-9.0
Melting Range: 190-192°C

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White Powder
  • FTIR: Conforms to reference
  • Optical ABS @ 260 NM:  0.1
  • Optical ABS @ 280 NM:  0.1

 

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PR1MA PBS [Phosphate Buffered Saline]

 

Common Name: Phosphate Buffered Saline
CAS Number: 7647-14-5; 7447-40-7; 7558-79-4; 7778-77-0
Solubility: Water
Storage Temp: Room Temperature

 

PBS, 100 mL Tablets refer to pre-weighed tablets that are used to prepare a specific volume of PBS (Phosphate Buffered Saline) solution. 

PBS is a widely used buffer solution in biological and biochemical research. PBS serves as a versatile buffer for various applications, including cell culture, immunohistochemistry, protein and nucleic acid research, and diagnostic assays. It helps maintain a stable pH and osmolarity, providing optimal conditions for biological reactions and preserving the integrity of cells and biomolecules. 

Each tablet contains pre-determined amounts of sodium chloride, sodium phosphate dibasic, and potassium phosphate monobasic. When dissolved in distilled water or a suitable solvent, the tablet dissolves, releasing the components, and instantly forms a ready-to-use PBS solution with a total volume of 100 mL.

These tablets ensure accurate and consistent preparation of PBS, eliminating the need for weighing and measuring individual components. They are designed to simplify the process and save time in laboratory workflows.

  

Research or further manufacturing use only, not for food or drug use.

 

Appearance: White, Concave Tablets

Clarity and Color of Solution: Clear colorless solution (No Cloudy) 5 tablets in 500 ml H2O

Hardness Test: Non-Fragile, No Chips, No Broken Tablets

pH (1 Tablet/ 100 ml Water): 7.3 - 7.5

 

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PR1MA
  G-418 Disulfate
CAS Number: 108321-42-2

Molecular Weight
: 692.7
Chemical Formula: C20H40N4O10  2H2SO4
Solubility: Water
Storage Temp: 2-8°C
 
Aminoglycoside antibiotic used as a selective agent in transformation experiments. Alternative for Kanamycin in monocots eg: rice, lollum and graminea which are highly resistant to the latter. Optical rotation +121.2°. Cell culture tested on Bacillus subtilus.
 
Research or further manufacturing use only, not for food or drug use.
  • Appearance: White Powder
  • Absorbance @ 280nm (1mg/mL in H2O):  0.015 nm
  • Absorbance @ 570nm (100mg/mL in H2O):  0.10 nm
  • Ammonia:  1.0 %
  • HPLC (Purity) : Report %
  • Loss on Drying:  10.0 %
  • pH: 5.0 - 6.0
  • Potency (Dry basis):  700 µg/mg
  • Purity (TLC-Spot):  3 Spots
  • Solubility (100mg/ml in H2O): Colorless Solution
  • Specific Rotation: +104 - +121 °
  • Non Animal Origin: The product does not come in contact with materials of animal origin.

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PR1MA Potassium Phosphate, Dibasic

CAS Number: 7758-11-4
Molecular Weight: 174.2
Chemical Formula: K2HPO4
Solubility: Water
Storage Temp: Room Temperature

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White Powder
  • Aerobic Plate Count:  ≤  1000 CFU/g
  • Assay:  98.0 %
  • Chloride (Cl):  0.003 %
  • Heavy Metals :  5 ppm
  • Insoluble Matter:  0.01 %
  • Iron:  0.001 %
  • Loss on Drying:  1.0 %
  • Nitrogen Compound:  0.001 %
  • pH (5% Solution): 8.5 - 9.6
  • Sodium (Na):  0.05 %
  • Sulfate:  0.005 %
  • Total Coliforms: Negative
  • Yeast and Mold Count:  100 CFU/g

Proudly made in the USA! Click here for more made in America products!
 
 

[3-[(3-Cholamidopropyl)-dimethylammonio]-1-propanesulfonate]


PR1MA
 CHAPS [3-[(3-Cholamidopropyl)-dimethylammonio]-1-propanesulfonate]

Common Name: 3-[(Cholamidopropyl) dimethylammonio]-1-propanesulfonate
CAS Number: 75621-03-3
Molecular Weight: 614.9
Chemical Formula: C32H58N2SO7
Solubility: Water
Storage Temp: Room Temperature

Zwitterionic, non-denaturing detergent for solubilizing membrane proteins.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White Powder
  • Identification: Pass Test
  • Moisture :  2.0 %
  • Purity:  99 %
  • RNase/DNase/Protease: None Detected
  • Solubility (20%, water): Complete / Colorless
  • TLC (silica gel: methanol/NH4OH = 95/5): One Spot

Proudly made in the USA! Click here for more made in America products!

PR1MA  Ticarcillin Disodium Salt

CAS Number: 4697-14-7
Molecular Weight: 428.39
Chemical Formula: C15H14N2Na2O 6S2
Solubility: Water
Storage Temp: 2-8°C

Inhibits bacterial cell wall synthesis. Highly active against gram negative bacteria such as Agrobacterium strains. -Lactamase inhibitor. Hygroscopic.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White to yellow crystalline powder
  • Assay (Ticarcillin Content, dried basis):  80.0 %
  • Assay (Ticarcillin, dried basis):  800 µg/mg
  • Heavy Metals :  10 ppm
  • Identification: Pass Test
  • pH: 6.0 - 8.0
  • Related Substances (Largest Impurity):  4.0 %
  • Related Substances (Second Major Impurity) :  2.5 %
  • Residual Solvents: Meets the requirements
  • Specific Rotation: +172 - +187 °
  • Sterility: Meet the requirements
  • Water:  6.0 %
  • Bacterial Endotoxins :  0.05 EU/mg
  • Grade: Conforms to USP Specifications

Proudly made in the USA! Click here for more made in America products!
 
 

PR1MA Malt Extract Agar, Powder

CAS Number: 8002-48-0
Solubility: Water with Heat
Storage Temp: Room Temperature

Malt Extract Agar is used for the isolation, cultivation, and maintenance of yeasts and molds. Formulation is similar to Malt Extract Broth with the addition of agar and glycerol. This broth medium was recommended by Thom and Church for the cultivation of fungi. Its low acidity inhibits the growth of most bacteria. The high carbohydrate content enhances the rapid growth of fungi and yeasts.

Dissolve 33.6 grams in 1 liter of distilled water. Sterilize by autoclaving for 15 minutes.

Ingredients:

  • Maltose: 12.75 g/L
  • Dextrin: 2.75 g/L
  • Glycerol: 2.35 g/L
  • Peptone: 0.78 g/L
  • Agar: 15.0 g/L
  • Final pH (25°C): 4.6 ± 0.2

Research or further manufacturing use only, not for food or drug use.

  • DCM Appearance: Homogeneous with soft lumps, free flowing, light beige
  • PPM Appearance: Trace to moderate haze, off white to pale yellow
  • pH(3.36% Solution @ 25 oC: 4.5 - 4.9
  • Microorganism (Aspergillus niger ATCC 16404): Growth
  • Microorganism (Candida albicans ATCC 10231): Growth
  • Microorganism (Penicillium Roquefortii ATCC 10110): Growth
  • Microorganism (Saccharomyces cerevisiae ATCC 9763): Growth
  • Microorganisms (Microsporum Audouinii ATCC 42558): Growth
  • Microorganisms (Microsporum Canis ATCC 36299): Growth
  • Microorganism (Trichophyton Mentagrophytes ATCC 9533): Growth

Proudly made in the USA! Click here for more made in America products!
 
 

PR1MA Tryptone, Granulated

CAS Number: 91079-40-2
Solubility: Water
Storage Temp: Room Temperature

Pancreatic digest of casein. Material is specially granulated to reduce dust and increase solubility rate.

Ingredients:

  • Total Nitrogen (TN): ~12.7%
  • Amino Nitrogen (AN): ~3.7%
  • AN/TN Ratio: 0.26
  • Sodium Chloride: ~0.4%
  • Tryptophan: 1.0
  • pH (1% solution): ~7.3%
Research or further manufacturing use only, not for food or drug use.
  • Appearance: Light Beige
  • Amino Nitrogen Content:  3.5 %
  • Ash:  15.0 %
  • Loss on Drying:  5.0 %
  • pH (2.0% Solution @ 25 oC): 6.5 - 7.5
  • Total Nitrogen Content:  10 %
  • Enzyme Species: Porcine
  • Raw Material Species: Bovine

Proudly made in the USA! Click here for more made in America products!
 
 

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