Molecular Biology Reagents/Chemicals

MIDSCI™ gives you ultra-pure, cost-competitive molecular biology reagents and lab chemicals for life science research; often with significant cost savings over Sigma Aldrich and other lab reagent providers.

PR1MA™ Chemicals can help with a wide variety of research-based requirements.
 
Use our glycerol for polyacrylamide gel electrophoresis. Eliminate Agrobacterium after inoculation with the carbenicillin we carry. Or detect β-Galactosidase activity with X-GAL which, when combined with the IPTG we stock, can be used to detect lac gene expression in cloning applications.
 
We also carry Luria Broth (Lennox), for the propagation and maintenance of E. coli; DTT, in order to maintain –SH groups in a reduced state; and high gel strength agar for tissue culture and microbiological work.

|◀ 1 - 12 of 179 ▶|
View:

PR1MA Agarose

 

Agarose is a low EEO, multi-purpose, standard melting point agarose that yields high resolution sharp DNA bands with high clarity and low background. Its optimized gel strength enhances ease of gel processing and handling. Manufactured using innovative organic solvent free manufacturing process that is more environment friendly.

 

Application: 

  • Ideal for routine DNA and RNA gel electrophoresis and blotting

Features:

  • High purity and Multi-purpose;
  • Low background and high resolution;
  • Optimized gel strength for ease of gel preparation

Specifications


AppearanceWhite, Fine, Homogeneous Powder
Water7% max.
SolubilityClear colorless solution at
1G plus 100 mL water
Gel strength(1% Gel)1,380 g/cm2
EEO(Electroendosmosis(-Mr)0.13 max.
Sulfate0.15 max.
Ash0.5 max.
Gelling point (1.5% Gel)36°C±1.5°C
GradeBiology grade
Item#:
ASMIDSCI500

PR1MA Tricine, Ultra Pure [N-[Tris (Hydroxymethyl) Methyl] Glycine]

Common Name: N-[Tris (Hydroxymethyl) Methyl] Glycine
CAS Number: 5704-04-1
Molecular Weight: 179.17
Chemical Formula: C6H13NO5
Solubility: Water
Storage Temp: Room Temperature

  • pKa @ 25°C: 8.1-8.2
  • Melting Range: 182-188°C
Research or further manufacturing use only, not for food or drug use.
  • Appearance: White Crystalline Powder
  • Assay (Titration, dried basis):  99.0 %
  • Heavy Metals :  5 ppm
  • pH (1% Solution): 4.6 - 5.3
  • Solubility (1M Solution in H2O): Clear
  • UV Absorbance 260nm (1 mol/L, 1cm ):  0.04
  • UV Absorbance 280nm (1 mol/L, 1cm ):  0.02
  • Water Content :  0.1 %

Proudly made in the USA! Click here for more made in America products!
 
 

PR1MA Ampicillin, Sodium Salt

CAS Number: 69-52-3
Molecular Weight: 371.4
Chemical Formula: C16H18N3O4SNa
Solubility: Water
Storage Temp: 2-8°C

Inhibitor of bacterial cell wall synthesis resulting in lysis or deformation of the bacterium. Inhibits crosslinking of peptidoglycan by binding and inactivating transpeptidases. Highly active against gram negative bacteria. B-Lactamase sensitive. Plant cell culture tested. Widely used as a selective agent for transformation of plasmid encoding for b-Lactamase production such as pBR322 or pUC (Ampr).

  • Appearance: White Crystalline Powder
  • Assay (Anhydrous Substance) : 91.0 - 102 %
  • Bacterial Endotoxins :  0.15 IU/mg
  • Dimethylaniline:  0.002 %
  • Identification: Conform
  • Methylene Chloride :  0.2 %
  • pH (1% Solution): 8.0 - 10.0
  • Solubility (50mg/ml in Water): Soluble
  • Specific Optical Rotation: +258 - +287 °
  • Sterility: Conform
  • Water:  2.0 %

Proudly made in the USA! Click here for more made in America products!
 
 

PR1MA TBE 10x Solution

Common Name: TRIS-borate-EDTA 10X Solution
CAS Number: 77-86-1; 10043-35-3; 6381-92-6; 7732-18-5
Solubility: Water
Storage Temp: Room Temperature

TBE is a common buffer used in gel electrophoresis.

Supplied as a 1X concentrated solution containing:

89mM Tris Base
89mM Boric Acid
2mM EDTA disodium salt
pH (1X solution @ 25°:C): 8.3+0.1
Shipping Weight: 2.6 lbs

Shipping Dimensions: 3.50 x 9.00 x 3.50
  • Appearance: Clear, Colorless Solution
  • pH (1X Solution): 8.15 - 8.45
Research or further manufacturing use only, not for food or drug use.
 

Proudly made in the USA! Click here for more made in America products!
 
 



PR1MA
 Agarose SS, Super High Strength, Molecular Biology Grade

CAS Number: 9012-36-6
Solubility: Water
Storage Temp: Room Temperature

For DNA/RNA fragments >1000 bp. Ideal for separating larger molecules. DNAse/RNase free.

Gel Strength, 1%: >1800 g/cm2
Gel Strength, 1.5%: >3200 g/cm2
Gelling Temperature: 35-37°C
Melting Temperature: 87-89°C
Sulfate: <0.12%
EEO: <0.13%

Research or further manufacturing use only, not for food or drug use.

  • Appearance: Fine white homogeneous powder
  • DNase/ RNase: Not Detected
  • Gelling Temperature:  10 oC
  • Melting Point: 80 - 100 oC

Proudly made in the USA! Click here for more made in America products!
 
 

Item#:
ASPRIMAAGASHS
[Benzyl Penicillin Sodium Salt]

PR1MA Penicillin G Sodium Salt [Benzyl Penicillin Sodium Salt]

Common Name: Benzyl Penicillin Sodium Salt
CAS Number: 69-57-8
Molecular Weight: 356.4
Chemical Formula: C16H17N2NaO4S
Solubility: Water
Storage Temp: 2-8°C

Inhibitor of bacterial cell wall synthesis. Active against gram positive and some gram negative bacteria. β-Lactamase sensitive.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White crystalline powder
  • Potency : 1500 - 1750 U/mg
  • Assay: 96.0 - 102.0 %
  • Identification: Pass Test
  • Loss on Drying:  1.0 %
  • pH: 5.5 - 7.5
  • Specific Optical Rotation: +285 - +310 °
  • Absorbance 264 nm: 0.80 - 0.88
  • Absorbance 280nm:  0.10
  • Absorbance 325nm:  0.10
  • Related Substances: Conforms to standard
  • Bacterial Endotoxins : Conforms to standard
  • Sterility: Conforms to standard
  • Country of Origin : Report

Proudly made in the USA! Click here for more made in America products!
 
 

PR1MA Novobiocin Sodium Salt

CAS Number: 1476-53-5

Molecular Weight: 634.61

Chemical Formula: C31H35N2NaO11

Solubility: Water

Storage Temp: 2-8°C

 

Novobiocin Sodium Salt is an antibiotic that is a member of the coumarin class of antibiotics and is mainly effective against Gram-positive bacteria. In microbiology and molecular biology research, Novobiocin Sodium Salt is used as a selective agent.

Research or further manufacturing use only, not for food or drug use. 

  • Appearance: White to yellow powder
  • Description - USP: Meets Test
  • Novobiocin (Dried Basis)-USP: ≥ 850 µg/mg
  • Identification - USP: Positive
  • Specific Rotation Dried Basis - USP: -58 to -50  o
  • Identification A - USP: Positive
  • Identification B - USP: Positive
  • pH(USP): 6.5 - 8.5
  • Loss on Drying - USP: ≤ 6.0 %
  • Residue on Ignition - USP: 10.5 - 12.0 %
  • Crystallinity - USP: Meets Test
  • Acetone - USP: ≤ 5000 ppm
  • Isooctane - USP: ≤ 5000 ppm
  • Assay Use Value: ≥ 845 ug/mg
  • Impurities Total HPLC Normal: ≤ 10.0 %
  • Impurities Unspecified: ≤ 0.2 %
  • Novobiocin-2,3-Carbonate: ≤ 0.6 %
  • A-Ring Amide: ≤ 0.3 %
  • Decarbamyl Novobiocin: ≤ 5.0 %
  • Isonovobiocin: ≤ 3.0 %
  • A-Ring Hydroxy: ≤ 1.0 %
  • Carbamyl Novobiocin: ≤ 3.0 %
  • DES-2-Isobutenyl: ≤ 0.5 %
  • DES-A-Ring Alpha-Keto-Carboxylic Acid: ≤ 0.5 %
  • O-Desmethylnovobiocin: ≤ 0.3 %
  • O-Desmethyl descarbamyl novobiocin: ≤ 0.3 %
  • A-Ring Carbamyl: ≤ 0.5 %
  • 8- Desmethyl novobiocin: ≤ 0.2 %
  • Carbamyl Novobiocin + Decarbamyl Novobiocin + Isonovobiocin: ≤ 11.0 %
  • BSE/TSE Free: BSE/TSE Free
  • Country of Origin: USA 

Proudly made in the USA! Click here for more made in America products!
 
 

PR1MA Phleomycin 20 mg / mL Solution

CAS Number: 11006-33-0
Molecular Weight: 1526.5 g/mol
Chemical Formula: C55H85O21N20S 2Cu  HCl
Solubility: Water
Storage Temp: -20°C

Supplied at a concentration of 20 mg/ml.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: Clear Blue Solution
  • Concentration: = 20 mg/ml
  • Odor: None
  • Solubility (20 mg/ml in Water): Freely soluble
  • Sterile Filtered: Pass test
  • UV Absorbance 244nm (200 ug/ml): 0.75 - 1.05

Proudly made in the USA! Click here for more made in America products!
 
 

PR1MA TBE Buffer 10x Powder

Common Name: TRIS-Borate-EDTA
CAS Number: 77-86-1; 10043-35-3;6381-92-6/60-00-4
Solubility: Water
Storage Temp: Room Temperature

Dry homogeneous blend of molecular biology grade TRIS base, Boric Acid and EDTA.

For use in gel electrophoresis.

Shipping Weight: 0.5 lbs
Shipping Dimensions: 3.50 x 3.75 x 3.50
Appearance: White Powder
pH (1 X Solution): 8.20 - 8.40
Solubility: Clear and Colorless

Research or further manufacturing use only, not for food or drug use.
 

Proudly made in the USA! Click here for more made in America products!
 
 

[N-(2-Hydroxyethyl) piperazine-N'-(2-ethanesulfonic acid)]

PR1MA  HEPES, Sodium Salt [N-(2-Hydroxyethyl) piperazine-N'-(2-ethanesulfonic acid)]

Common Name: N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)
CAS Number: 75277-39-3
Molecular Weight: 260.3
Chemical Formula: C8H17N2O4SNa
Solubility: Water
Storage Temp: Room Temperature

  • pKa (20°C): 7.45-7.65
  • pH Range: 6.8-8.2

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White crystalline powder
  • Assay (Titration, dried basis):  98.0 %
  • Chloride (Cl):  0.1 %
  • Heavy Metals (as Pb):  1 ppm
  • Loss on Drying @ 105 oC, 3hrs:  2.0 %
  • pH (1% aqueous @ 25 oC): 9.4 - 11.0
  • pKa @ 20°C: 7.45 - 7.65
  • Solubility (1M Solution in H2O): Clear, complete and colorless
  • UV Absorbance @ 250nm 1M:  1.0

Proudly made in the USA! Click here for more made in America products!
 
 

PR1MA Hygromycin B Powder  

CAS Number: 31282-04-9
Molecular Weight: 527.5
Chemical Formula: C20H37N3O13
Solubility: Water
Storage Temp: 2-8°C

Aminoglycoside antibiotic inhibits protein synthesis in prokaryote and eukaryote cells. Allows selection of cells expressing the Hygromycin B resistant gene. Working concentrations vary from 30-1000 ug/mg, depending on cell lines and growth conditions.

Shipping Weight: 0 lbs

Shipping Dimensions: 1.25 x 1.75 x 1.25

Research or further manufacturing use only, not for food or drug use.
 

Proudly made in the USA! Click here for more made in America products!
 
 

PR1MA PBS / Tween 20 10X Solution

CAS Number: 7558-79-4, 7778-77-0, 7647-14-5, 7447-40-7, 9005-64-5, 7732-18-5
Solubility: Water
Storage Temp: Room Temperature

Research or further manufacturing use only, not for food or drug use.

  • Appearance: Clear, yellow solution
  • pH: 7.2 - 7.6

Proudly made in the USA! Click here for more made in America products!
 
 

|◀ 1 - 12 of 179 ▶|
View: