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				Molecular Biology Reagents/Chemicals
MIDSCI™ gives you ultra-pure, cost-competitive molecular biology reagents and lab chemicals for life science research; often with significant cost savings over Sigma Aldrich and other lab reagent providers.
PR1MA™ Chemicals can help with a wide variety of research-based requirements.
 
Use our glycerol for polyacrylamide gel electrophoresis. Eliminate Agrobacterium after inoculation with the carbenicillin we carry. Or detect β-Galactosidase activity with X-GAL which, when combined with the IPTG we stock, can be used to detect lac gene expression in cloning applications.
 
We also carry Luria Broth (Lennox), for the propagation and maintenance of E. coli; DTT, in order to maintain –SH groups in a reduced state; and high gel strength agar for tissue culture and microbiological work.
PR1MA™ Agarose
Agarose is a low EEO, multi-purpose, standard melting point agarose that yields high resolution sharp DNA bands with high clarity and low background. Its optimized gel strength enhances ease of gel processing and handling. Manufactured using innovative organic solvent free manufacturing process that is more environment friendly.
Application: 
- Ideal for routine DNA and RNA gel electrophoresis and blotting
Features:
- High purity and Multi-purpose;
- Low background and high resolution;
- Optimized gel strength for ease of gel preparation
Specifications
| Appearance | White, Fine, Homogeneous Powder | 
| Water | 7% max. | 
| Solubility | Clear colorless solution at 1G plus 100 mL water | 
| Gel strength(1% Gel) | 1,380 g/cm2 | 
| EEO(Electroendosmosis(-Mr) | 0.13 max. | 
| Sulfate | 0.15 max. | 
| Ash | 0.5 max. | 
| Gelling point (1.5% Gel) | 36°C±1.5°C | 
| Grade | Biology grade | 
PR1MA™ Tricine, Ultra Pure [N-[Tris (Hydroxymethyl) Methyl] Glycine]
    Common Name: N-[Tris (Hydroxymethyl) Methyl] Glycine
CAS Number: 5704-04-1
Molecular Weight: 179.17
Chemical Formula: C6H13NO5
Solubility: Water
Storage Temp: Room Temperature
- pKa @ 25°C: 8.1-8.2
- Melting Range: 182-188°C
- Appearance: White Crystalline Powder
- Assay (Titration, dried basis): ≥ 99.0 %
- Heavy Metals : ≤ 5 ppm
- pH (1% Solution): 4.6 - 5.3
- Solubility (1M Solution in H2O): Clear
- UV Absorbance 260nm (1 mol/L, 1cm ): ≤ 0.04
- UV Absorbance 280nm (1 mol/L, 1cm ): ≤ 0.02
- Water Content : ≤ 0.1 %
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Tricine, Ultra, Pure, [N-[Tris, (Hydroxymethyl), Methyl], Glycine], PR1MA, MIDSCI, Laboratory Supplies, High-Quality, Research, Consumables, Reagents, Equipment, Customer Service, Quick Shipping, Satisfaction Guarantee, Personalized Solutions, Universities
PR1MA™ Ampicillin, Sodium Salt
    CAS Number: 69-52-3
Molecular Weight: 371.4
Chemical Formula: C16H18N3O4SNa
Solubility: Water
Storage Temp: 2-8°C
    Inhibitor of bacterial cell wall synthesis resulting in lysis or
    deformation of the bacterium. Inhibits crosslinking of peptidoglycan by
    binding and inactivating transpeptidases. Highly active against gram
    negative bacteria. B-Lactamase sensitive. Plant cell culture tested. Widely
    used as a selective agent for transformation of plasmid encoding for
    b-Lactamase production such as pBR322 or pUC (Ampr).
- Appearance: White Crystalline Powder
- Assay (Anhydrous Substance) : 91.0 - 102 %
- Bacterial Endotoxins : ≤ 0.15 IU/mg
- Dimethylaniline: ≤ 0.002 %
- Identification: Conform
- Methylene Chloride : ≤ 0.2 %
- pH (1% Solution): 8.0 - 10.0
- Solubility (50mg/ml in Water): Soluble
- Specific Optical Rotation: +258 - +287 °
- Sterility: Conform
- Water: ≤ 2.0 %
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Ampicillin, Sodium, Salt, PR1MA, MIDSCI, Laboratory Supplies, High-Quality, Research, Consumables, Reagents, Equipment, Customer Service, Quick Shipping, Satisfaction Guarantee, Personalized Solutions, Universities
PR1MA™ TBE 10x Solution
CAS Number: 77-86-1; 10043-35-3; 6381-92-6; 7732-18-5
Solubility: Water
Storage Temp: Room Temperature
- Appearance: Clear, Colorless Solution
- pH (1X Solution): 8.15 - 8.45
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TBE, 10x, Solution, PR1MA, MIDSCI, Laboratory Supplies, High-Quality, Research, Consumables, Reagents, Equipment, Customer Service, Quick Shipping, Satisfaction Guarantee, Personalized Solutions, Universities
PR1MA™ Agarose SS, Super High Strength, Molecular Biology Grade
    CAS Number: 9012-36-6
Solubility: Water
Storage Temp: Room Temperature
    For DNA/RNA fragments >1000 bp. Ideal for separating larger molecules.
    DNAse/RNase free.
    Gel Strength, 1%:
    >1800 g/cm2
    
    Gel Strength, 1.5%:
    >3200 g/cm2
    
    Gelling Temperature:
    35-37°C
    
    Melting Temperature:
    87-89°C
    
    Sulfate:
    <0.12%
    
    EEO:
    <0.13%
    Research or further manufacturing use only, not for food or drug use.
- Appearance: Fine white homogeneous powder
- DNase/ RNase: Not Detected
- Gelling Temperature: ≥ 10 oC
- Melting Point: 80 - 100 oC
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Agarose, SS, Super, High, Strength, PR1MA, MIDSCI, Laboratory Supplies, High-Quality, Research, Consumables, Reagents, Equipment, Customer Service, Quick Shipping, Satisfaction Guarantee, Personalized Solutions, Universities
PR1MA™ Penicillin G Sodium Salt [Benzyl Penicillin Sodium Salt]
    Common Name: Benzyl Penicillin Sodium Salt
CAS Number: 69-57-8
Molecular Weight: 356.4
Chemical Formula: C16H17N2NaO4S
Solubility: Water
Storage Temp: 2-8°C
    Inhibitor of bacterial cell wall synthesis. Active against gram positive
    and some gram negative bacteria. β-Lactamase sensitive.
    Research or further manufacturing use only, not for food or drug use.
- Appearance: White crystalline powder
- Potency : 1500 - 1750 U/mg
- Assay: 96.0 - 102.0 %
- Identification: Pass Test
- Loss on Drying: ≤ 1.0 %
- pH: 5.5 - 7.5
- Specific Optical Rotation: +285 - +310 °
- Absorbance 264 nm: 0.80 - 0.88
- Absorbance 280nm: ≤ 0.10
- Absorbance 325nm: ≤ 0.10
- Related Substances: Conforms to standard
- Bacterial Endotoxins : Conforms to standard
- Sterility: Conforms to standard
- Country of Origin : Report
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PR1MA™ Novobiocin Sodium Salt
CAS Number: 1476-53-5
Molecular Weight: 634.61
Chemical Formula: C31H35N2NaO11
Solubility: Water
Storage Temp: 2-8°C
Novobiocin Sodium Salt is an antibiotic that is a member of the coumarin class of antibiotics and is mainly effective against Gram-positive bacteria. In microbiology and molecular biology research, Novobiocin Sodium Salt is used as a selective agent.
Research or further manufacturing use only, not for food or drug use.
- Appearance: White to yellow powder
- Description - USP: Meets Test
- Novobiocin (Dried Basis)-USP: ≥ 850 µg/mg
- Identification - USP: Positive
- Specific Rotation Dried Basis - USP: -58 to -50 o
- Identification A - USP: Positive
- Identification B - USP: Positive
- pH(USP): 6.5 - 8.5
- Loss on Drying - USP: ≤ 6.0 %
- Residue on Ignition - USP: 10.5 - 12.0 %
- Crystallinity - USP: Meets Test
- Acetone - USP: ≤ 5000 ppm
- Isooctane - USP: ≤ 5000 ppm
- Assay Use Value: ≥ 845 ug/mg
- Impurities Total HPLC Normal: ≤ 10.0 %
- Impurities Unspecified: ≤ 0.2 %
- Novobiocin-2,3-Carbonate: ≤ 0.6 %
- A-Ring Amide: ≤ 0.3 %
- Decarbamyl Novobiocin: ≤ 5.0 %
- Isonovobiocin: ≤ 3.0 %
- A-Ring Hydroxy: ≤ 1.0 %
- Carbamyl Novobiocin: ≤ 3.0 %
- DES-2-Isobutenyl: ≤ 0.5 %
- DES-A-Ring Alpha-Keto-Carboxylic Acid: ≤ 0.5 %
- O-Desmethylnovobiocin: ≤ 0.3 %
- O-Desmethyl descarbamyl novobiocin: ≤ 0.3 %
- A-Ring Carbamyl: ≤ 0.5 %
- 8- Desmethyl novobiocin: ≤ 0.2 %
- Carbamyl Novobiocin + Decarbamyl Novobiocin + Isonovobiocin: ≤ 11.0 %
- BSE/TSE Free: BSE/TSE Free
- Country of Origin: USA
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PR1MA™ Phleomycin 20 mg / mL Solution
    CAS Number: 11006-33-0
Molecular Weight: 1526.5 g/mol
Chemical Formula: C55H85O21N20S    2Cu • HCl
Solubility: Water
Storage Temp: -20°C
Supplied at a concentration of 20 mg/ml.
    Research or further manufacturing use only, not for food or drug use.
- Appearance: Clear Blue Solution
- Concentration: = 20 mg/ml
- Odor: None
- Solubility (20 mg/ml in Water): Freely soluble
- Sterile Filtered: Pass test
- UV Absorbance 244nm (200 ug/ml): 0.75 - 1.05
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Phleomycin, 20mg/ml, Solution, PR1MA, MIDSCI, Laboratory Supplies, High-Quality, Research, Consumables, Reagents, Equipment, Customer Service, Quick Shipping, Satisfaction Guarantee, Personalized Solutions, Universities
PR1MA™ TBE Buffer 10x Powder
CAS Number: 77-86-1; 10043-35-3;6381-92-6/60-00-4
Solubility: Water
Storage Temp: Room Temperature
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PR1MA™ HEPES, Sodium Salt [N-(2-Hydroxyethyl) piperazine-N'-(2-ethanesulfonic acid)]
    Common Name: N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)
CAS Number: 75277-39-3
Molecular Weight: 260.3
Chemical Formula: C8H17N2O4SNa
Solubility: Water
Storage Temp: Room Temperature
- pKa (20°C): 7.45-7.65
- pH Range: 6.8-8.2
Research or further manufacturing use only, not for food or drug use.
- Appearance: White crystalline powder
- Assay (Titration, dried basis): ≥ 98.0 %
- Chloride (Cl): ≤ 0.1 %
- Heavy Metals (as Pb): ≤ 1 ppm
- Loss on Drying @ 105 oC, 3hrs: ≤ 2.0 %
- pH (1% aqueous @ 25 oC): 9.4 - 11.0
- pKa @ 20°C: 7.45 - 7.65
- Solubility (1M Solution in H2O): Clear, complete and colorless
- UV Absorbance @ 250nm 1M: ≤ 1.0
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PR1MA™ Hygromycin B Powder
Molecular Weight: 527.5
Chemical Formula: C20H37N3O13
Solubility: Water
Storage Temp: 2-8°C
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Hygromycin, B, Powder,PR1MA, MIDSCI, Laboratory Supplies, High-Quality, Research, Consumables, Reagents, Equipment, Customer Service, Quick Shipping, Satisfaction Guarantee, Personalized Solutions, Universities
PR1MA™ PBS / Tween 20 10X Solution
    CAS Number: 7558-79-4, 7778-77-0, 7647-14-5, 7447-40-7, 9005-64-5,
    7732-18-5
Solubility: Water
Storage Temp: Room Temperature
    Research or further manufacturing use only, not for food or drug use.
- Appearance: Clear, yellow solution
- pH: 7.2 - 7.6
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PBS/Tween, 20, 10X, Solution, PR1MA, MIDSCI, Laboratory Supplies, High-Quality, Research, Consumables, Reagents, Equipment, Customer Service, Quick Shipping, Satisfaction Guarantee, Personalized Solutions, Universities
 
					
				

