PR1MA™  Thiamine Hydrochloride [Vitamin B1]

Common Name: Vitamin B1
CAS Number: 67-03-8
Molecular Weight: 337.3
Chemical Formula: C₁₂H₁₇CIN₄OS • HCl
Solubility: Water
Storage Temp: 2-8°C

Research or further manufacturing use only, not for food or drug use.

• Appearance: White Crystalline Powder
• Appearance of Solution: Clear, no more color than reference solution Y7 or GY7
• Identification: Should be positive reaction
• Identification (IR): Consistent with the reference IR spectra
• pH: 2.7 - 3.3
• Sulfates: ≤ 300 ppm
• Limit of Nitrate: No brown ring is produced at the junction of the two layers
• Lead: ≤ 2 ppm
• Water: ≤ 5.0 %
• Residue on Ignition: ≤ 0.1 %
• Absorbance of Solution: ≤ 0.025
• Residual Solvents (Methanol): ≤ 0.3 %
• Residual Solvents (Ethanol): ≤ 0.5 %
• Related Substance (Impurity A): ≤ 0.15 %
• Related Substance (Impurity B): ≤ 0.3 %
• Related Substance (Impurity C): ≤ 0.15 %
• Related Substance (Any Other Impurity): ≤ 0.1 %
• Related Substance (Total impurities): ≤ 0.5 %
• Related Compounds: ≤ 1.0 %
• Chromatographic Purity: ≤ 1.0 %
• Assay: 98.5 - 101.0 %
• Staphylococcus Aureus: Negative
• Salmonella: Negative
• E.Coli: Negative
• Total Plate Count: ≤ 1000 cfu/g
• Yeast and Mold: ≤ 100 cfu/g
• Material Source: Acrylonitrate; Acetamidine HCl; Chlorine Ester
• Production Method: Chemical Synthesis
• BSE/TSE Free: BSE/TSE Free
• Non Animal Origin: Non Animal Origin
• Conclusion: This product complies with the specification of BP/USP/FCC.
• Country of Origin: Report

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PR1MA™ Yeast Extract, Powder

CAS Number: 8013-01-2
Solubility: Water
Storage Temp: Room Temperature

Dried yeast autolysate with a high content of amino nitrogen and water soluble B complex vitamins. Due to its low NaCl content this quality is well suited for tissue culture.

Ingredients:

• Total Nitrogen (TN): ~9.8%
• Amino Nitrogen (AN): ~5.1%
• AN/TN Ratio: ~0.52
• Sodium Chloride: ~0.3%
• pH (1% Solution): ~7.0

• Appearance: Light Beige Powder
• Amino Nitrogen: 4.5 - 5.8 %
• Ash Content: 11.50 - 16.0 %
• Dry Matter: ≥ 95.0 %
• Loss on Drying: ≤ 5.0 %
• pH (2.0% Solution @ 25 ^oC): 6.8 - 7.2
• Sodium Chloride: ≤ 0.5 %
• Total Nitrogen: 10.0 - 11.8 %
• Clostridium Perfringens Spores : Report cfu/g
• E.Coli: Report cfu/g
• Molds and Yeasts: Report cfu/g
• Pathenogenic Staphylococcus: Report cfu/g
• Salmonella : Report cfu/25g
• Standard Plate Count: Report cfu/g

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PR1MA™ 2XYT Broth, Capsules

CAS Number: 91079-40-2; 8013-01-2; 7647-14-5
Solubility: Water
Storage Temp: Room Temperature

Nutritionally enriched medium for the growth of recombinant strains of E. Coli and in the preparation and transformation of competent cells.

2XYT Broth packaged in convenient ready to use capsules. Each capsule contains 15.5 g 2XYT Broth.

Simply add 2 capsules to one liter distilled water and heat until completely dissolved. Sterilize by autoclaving at 121°C for 15 minutes. Final pH at 25°C: 6.8-7.2.

Ingredients:

• Digest Peptone: 16 g/l
• Yeast Extract: 10 g/l
• Sodium Chloride:5 g/l

Research or further manufacturing use only, not for food or drug use.

• Appearance: Capsule (Cream to beige free flowing powder)
• DCM Appearance: Capsule (Homogeneous, free flowing, beige)
• PPM Appearance: Capsule (Clear, no to light ppt, yellow)
• pH (3.1 % Solution @25 ^oC): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent
• Casein Peptone: 16.0 g/L
• Yeast Extract: 10.0 g/L
• Sodium Chloride: 5.0 g/l
• Description: Add 2 capsules 2XYT broth in 1 liter purified water until evenly dispersed. Boil for 1 minute with stirring to completely dissolve. Distribute and autoclave at 121^oC for 15 minutes.

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PR1MA™ 2XYT Broth, Powder

CAS Number: 91079-40-2; 8013-01-2; 7647-14-5
Solubility: Water
Storage Temp: Room Temperature

Nutritionally enriched medium for the growth of recombinant strains of E. Coli and in the preparation and transformation of competent cells.

Dissolve 31g in one liter distilled water. Heat solution to boiling with continuous stirring. Sterilize by autoclaving at 121°C for 15 minutes. Final ph @ 25°C: 6.8-7.2.

Ingredients:

• Digest Peptone: 16 g/l
• Yeast Extract: 10 g/l
• Sodium Chloride: 5 g/l

Research or further manufacturing use only, not for food or drug use.

• Appearance: Cream to beige free flowing powder
• DCM Appearance: Homogeneous free flowing, beige
• PPM Appearance: Clear,no to light ppt,yellow
• pH (3.1 % Solution @25 ^oC): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

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PR1MA™ Tryptone, Powder

CAS Number: 91079-40-2
Solubility: Water
Storage Temp: Room Temperature

Pancreatic digest of casein used to supplement media in cultivation of anaerobic bacteria.

pH (1% solution): 7.3.

Ingredients:

• Molecular Biology GradeTotal Nitrogen (TN): ~12.7%
• Amino Nitrogen (AN): ~3.7%
• AN/TN Ratio: 0.26
• Sodium Chloride: ~0.4%
• Tryptophan: ~1.0

• Appearance: Cream to beige free flowing powder
• Amino Nitrogen: ≥ 4.0 %
• Ash: ≤ 15 %
• Loss on Drying: ≤ 5.0 %
• Nitrites: Negative
• pH (2% water) @25°C: 6.5 - 7.5
• Total Nitrogen: ≥ 10 %
• Undigested Protein: Negative
• Broth Formulations (Growth Supporting Properties): Satisfactory
• Peptone Agar (Growth Supporting Properties): Satisfactory
• Proteoses: Positive
• Salmonella: Negative/25g
• Standard Plate Count : ≤ 1000 cfu/g
• Coliform: Negative
• E.Coli: Negative
• Molds and Yeasts: ≤ 100 cfu/g
• Raw Material Species: Bovine
• Enzyme Species: Porcine
• BSE/TSE Free: BSE/TSE Free
• Country of Origin : Report

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PR1MA™ TBE Buffer 10x Powder

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PR1MA™  HEPES, Sodium Salt [N-(2-Hydroxyethyl) piperazine-N'-(2-ethanesulfonic acid)]

Common Name: N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)
CAS Number: 75277-39-3
Molecular Weight: 260.3
Chemical Formula: C₈H₁₇N₂O₄SNa
Solubility: Water
Storage Temp: Room Temperature

• pKa (20°C): 7.45-7.65
• pH Range: 6.8-8.2

Research or further manufacturing use only, not for food or drug use.

• Appearance: White crystalline powder
• Assay (Titration, dried basis): ≥ 98.0 %
• Chloride (Cl): ≤ 0.1 %
• Heavy Metals (as Pb): ≤ 1 ppm
• Loss on Drying @ 105 ^oC, 3hrs: ≤ 2.0 %
• pH (1% aqueous @ 25 ^oC): 9.4 - 11.0
• pKa @ 20°C: 7.45 - 7.65
• Solubility (1M Solution in H₂O): Clear, complete and colorless
• UV Absorbance @ 250nm 1M: ≤ 1.0

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PR1MA™ Hygromycin B Powder  

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PR1MA™ Luria Broth Granulated [Miller's LB Broth]

Common Name: Miller's LB Broth
CAS Number: 91079-40-2; 8013-01-2; 7647-14-5
Solubility: Water
Storage Temp: Room Temperature

For propagation and maintenance of E. Coli. High Salt Formula. Molecular Biology Grade.

Dissolve 25 grams in 1 liter distilled water and adjust the pH to 7.2. Heat and frequently agitate to completely dissolve. Sterilize by autoclaving for 15 minutes. Material specially granulated to reduce dust and increase solubility rate.

Ingredients:

• Tryptone:10 g/L
• Sodium Chloride: 10 g/L
• Yeast Extract: 5 g/L

Research or further manufacturing use only, not for food or drug use.

• Appearance: Cream to beige granular powder
• DCM Appearance: Homogeneous, free flowing beige to yellow-beige
• PPM Appearance: Clear, yellow to amber no precipitate (ppt)
• Appearance of Solution: Prepared medium is yellow to gold and clear to moderately hazy
• pH (2.5% H₂O @25 °C): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent
• Casein Peptone: 10 g/L
• Sodium Chloride: 10 g/L
• Yeast Extract: 5 g/L

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PR1MA™ Urea, UltraPure (USP Grade)

CAS Number: 57-13-6
Molecular Weight: 60.06
Chemical Formula: CH₄N₂O
Solubility: Water
Storage Temp: Room Temperature

Research or further manufacturing use only, not for food or drug use.

• Appearance: White Crystalline Powder
• Assay: 99.0 - 100.5 %
• Identification (A, B): Pass Test
• Appearance of 20% Solution: Pass Test
• Melting Point: 132.5 to 134.5 ^oC
• Moisture (LOD): ≤ 1.0 %
• Alcohol- Insoluble Matter: ≤ 0.04 %
• Residue on Ignition: ≤ 0.1 %
• Chloride: ≤ 5 ppm
• Sulfate: ≤ 0.001 %
• Alkalinity: To pass test
• Ammonium: ≤ 0.05 %
• Urea Related Compound A: ≤ 0.1 %
• Individual Unspecified Impurities: ≤ 0.1 %
• Total impurities: ≤ 2.0 %
• Heavy Metals: ≤ 0.001 %
• Iron: ≤ 0.001 %
• Residual Solvents USP 467: Meets requirements
• BSE/TSE Free: BSE/TSE Free
• Grade: USP
• Country of Origin : Report

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PR1MA™ PBS / Tween 20 10X Solution

CAS Number: 7558-79-4, 7778-77-0, 7647-14-5, 7447-40-7, 9005-64-5, 7732-18-5
Solubility: Water
Storage Temp: Room Temperature

Research or further manufacturing use only, not for food or drug use.

• Appearance: Clear, yellow solution
• pH: 7.2 - 7.6

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CAS Number: 64485-93-4
Molecular Weight: 477.5
Chemical Formula: C₁₆H₁₆N₅NaO₇S ₂
Solubility: Water
Storage Temp: 2-8°C

Inhibitor of bacterial cell wall synthesis. Inhibits the crosslinking of peptidoglycan by binding and inactivating transpeptidases. High activity against gram negative bacteria. Often used for elimination of Agrobacterium species after inoculation. Resistant to B-Lactamase activity.

Non toxic to plant cells
Plant cell culture tested

Research or further manufacturing use only, not for food or drug use.

• Appearance: Off White to Pale Yellow Crystalline Powder
• Assay (Potency dry basis) : 916 - 964 µg/mg
• Bacterial Endotoxins : Meet the requirements
• Clarity of Solution: Meet the requirements
• Color of solution: Meet the requirements
• Identification (A, B, C): Pass Test
• Impurity - Individual: ≤ 1.0 %
• Loss on Drying: ≤ 3.0 %
• pH: 4.5 - 6.5
• Specific Rotation: +58 - +64 °
• Total impurities: ≤ 3.0 %

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