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PCR/qPCR

MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more.  If you are looking for any of these things, we can match almost all needs to all cyclers.  If you don’t find it easily contact us at tech@midsci.com and we will find it for you. 

96 or 384 we have it.  Non-Skirted, semi-skirted or full skirted, we have it.  0.1, 0.2, or 0.5 mL we have it.  A1, A12, H12, A24, P24 cuts, we have them.  Sybr© Green or probe alternative, we have it.  

Pro Tip: Colored plastics do not have any impact on DNA amplification.  But when setting up qPCR it is often recommended to use white plastics.  This will limit or prevent fluorescence refraction.  Light refraction can minimize sensitivity and consistency. 

Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips.  This will help optimize and reduce variance in the reactions.  Simple test, see the residual liquid left in tips?  This means some of the taq, dNTPs, sample, etc is left in the tip.  PR1MA tips help reduce that significantly and improve your consistency. 

Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate?  Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler.  Call us we have solutions.

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Need great (q)PCR Regents at a great price? Try PR1MA!  Click here to order.

2x Master Mix Kit (1.5 mM MgCl2) 

Bullseye Taq DNA Polymerase Mix is a ready-to-use 2x reaction mix. Simply add primers, template, and water to successfully carry out primer extensions and other molecular biology applications.

Bullseye Taq polymerase, the NH4+ buffer system, dNTPs, and magnesium chloride are conveniently present in the Taq DNA Polymerase Mix. (Inert Red Dye is present in BE180303 only)

Bullseye Taq DNA Polymerase Mix offers several advantages. Set up time is significantly reduced. The chance of contaminating component stocks is eliminated. Reduction of reagent handling steps leads to better reproducibility. Standard tests can be set up with the confidence that results will be consistent every time.

Composition of 2x Taq Master Mix

150 mM Tris-HCl pH 8.5, 40 mM (NH4)2S04, 3 mM MgCl2, 0.2% Tween 20Ò

4 mM dNTPs

2 units/µL AS ONE Taq polymerase

Inert Red Dye & Stabilizer (BE180303 only)

Item#:
ASPCRREAG3
Need great (q)PCR Regents at a great price? Try PR1MA!  Click here to order.





R-Taq DNA Polymerase 5 units/µL

Cat. No.

Units

10X Ammonium Buffer (MgCl2 15mM)

MgCl2

25 mM

BE200303

500

1.5 mL

1.5 mL

BE200304

1,000

2x 1.5 mL

2x 1.5 mL

BE200306

2,500

4x 1.5 mL

4x 1.5 mL

Store at -20°C. For in-vitro laboratory use only

 

General Description

Bullseye R-Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. R-Taq contains a red dye which provides easy and quick identification of reactions to which enzyme was added and allows confirmation of complete mixing. The inert dye has no effect on downstream processes. R-Taq is added directly to the reaction mix and is used in the same manner as standard Taq DNA Polymerase.

Bullseye R-Taq DNA Polymerase has both a 5'®3' DNA polymerase and a 5'®3' exonuclease activity. The enzyme lacks a 3'®5' exonuclease activity. R-Taq DNA Polymerase leaves an A-overhang, which makes the enzyme ideal for TA cloning.

  • High performance thermostable DNA polymerase
  • Red dye identifies tubes which contain enzyme and confirms complete mixing of reagents
  • Leaves an A-overhang

Unit Definition

One unit is defined as the amount that incorporates 10 nmoles of dNTPs into acid-precipitable form in 30 minutes at 72°C under standard assay conditions.

Storage Buffer

Enzyme is supplied in 20 mM Tris-HCl pH 8.3, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, inert dye, 0.5 % Tweenà 20, 0.5% NP40, 50% glycerol.

Component

Vol./reaction

Final Conc.

10X Ammonium Buffer

5 µL

1X

dNTP mix (12.5 mM each)

0.8 µL

0.2 mM each dNTP

Primer A

Variable

0.1-0.5 µM

Primer B

Variable

0.1-0.5 µM

R-Taq DNA Pol

1 mL

5 units/reaction

Distilled Water

Variable

- - - -

Template DNA

Variable

0.1-0.5 µg/reaction

TOTAL volume

50 µL

- - - -

Table 2. MgCl2 concentration

3. Mix the master mix thoroughly and dispense appropriate volumes into reaction tubes. Mix gently (e.g., by pipetting) the master mix up and down a few times.

4. Add template DNA (0.1-0.5 mg/reaction) to the individual tubes containing the master mix.

5. Program the thermal cycler according to the manufacturer's instructions.

For maximum yield and specificity, temperatures and cycling times should be optimized for each new template target or primer pair.

6. Place the tubes in the thermal cycler and start the reaction.

7. After primer extension, load 5-10 mL of a 50 mL reaction directly on an agarose gel for analysis.


Tween 20 is a registered trademark of ICI Americas, Inc.


Final MgCl2 conc.

in reaction (mM)

1.5

2.0

2.5

3.0

3.5

4.0

4.5

Additional volume

of 25 mM MgCl2

per reaction (µL):

0

1

2

3

4

5

6

10X Ammonium Reaction Buffer

Tris-HCl pH 8.5, (NH4)2S04, 15 mM MgCl2, 1% Tween 20

Quality Control

Endonuclease, exonuclease and priming activities are not detected after 3 hours incubation of 1 mg of pUC19 plasmid DNA and 0.5 µg EcoR I digested lambda phage DNA at 72°C in the presence of 40 units of R-Taq DNA Polymerase.

Suggested Protocol using R-Taq Polymerase

This protocol serves as a guideline. Optimal reaction conditions such as incubation times, temperatures, and amount of template DNA may vary and must be individually determined.

1. Thaw 10X Ammonium Buffer, dNTP mix, and primer solutions. It is important to mix the solutions completely before use to avoid localized concentrations of salts.

2. Prepare a master mix according to Table 1. The master mix typically contains all the components needed for extension except the template DNA.

The optimal MgCl2 concentration should be determined empirically but, in most cases a concentration of 1.5 mM, as provided in the 1X Ammonium Buffer, produces satisfactory results. Table 2 provides the volume of 25mM MgCl2 to add to the master mix if a higher MgCl2 concentration is required.

Table 1. Reaction components (master mix & template DNA)

Item#:
ASPCRREAG4
Item#:
ASSEALRAYACR
10 Mats/Bag, 5 Bags/Case
This item has been discontinued and replaced by item 

PCR SILICONE SEALING MAT - 96

  • Made of research-grade silicone
  • Scored wells for sample access
  • Perforations re-seal after access
  • Resists coring and tearing
  • Eliminates cross-talk between wells
  • Also works as a compression mat
  • Suitable for standard PCR thermocycling
  • Mats can be reused
  • For use with 96-well PCR plates
  • Printed alpha numeric grid for easy reference
  • Certified to be free of RNase, DNase, DNA, PCR inhibitors, and Pyrogen contamination.

Works as a plate seal (to eliminate cross contamination) and compression mat all-in-one!

Our compression molded 96-round plug, silicone sealing mats for PCR plates minimize sample evaporation in a wide range of temperatures. These mats are chemically resistant and reusable; containing no adhesives or extractables to contaminate samples.  Made of research-grade silicone, they can be autoclaved, and following a 10% bleach wash and ethanol rinse protocol may be reused.

Wells are scored (cut 80% of the way through, but not all of the way) to allow for sample access via pipettor, syringe, or probe.  These scored perforations will seal themselves, after penetration, ensuring an airtight closure to the well.
Item#:
MID-PCMATR-9-PRT
Your Price:
208.50
Each
This item is discontinued
Strips, 1000/cs for

These Rotocycler strips are perfectly designed to perform on Rotor-Gene instruments. Tube strips are packaged separately from cap strips. The frosted extensions on caps not only make them more efficient and secure during handling but also offer a convenient area for labeling. For individual use, tube and cap strips can easily be separated and used as individual units.

Item#:
T319-4N
Your Price:
511.43
Each
Thick Adhesive, Leaves No Residue Behind


Click to shop new and improved eXTReme™ 2 Seal Extra Strong Sealing Film


eXTReme™ Seal Sealing Film with Extra Strong Adhesive Minimizing Evaporation Without Residue

eXTReme™ Seal features an extra-strong thick adhesive to minimize evaporation, yet removes easily without leaving residue on the plate.

Features chamfered corners to eliminate plate overhang for robotics applications and tight storage conditions.

  • Certified DNase- & RNase- free
  • Ideal for use with raised-rim and non-skirted PCR plates
  • Available in a larger cut for flat-top semi-skirted and full-skirted PCR plates: eXTReme™ Seal, Large

Application

  • PCR
  • Robotics
Temperature Range
  • -20°C to +120°C

Helpful Tips

  • Selection of the proper film size will minimize evaporation
  • The film should not overhang or ride up the wall of a raised-rim plate
  • More complete coverage of skirted flat-top plates will reduce evaporation in the outer wells
  • End-tab removal before thermal cycling is highly recommended


Item#:
ASPCRSEALFILM
 
eXTReme Seal Features
Specs eXTReme Seal
Sealing Film Selection Guide by Application
Sealing Film Selection Guide by Attributes
Optically Transparent, Highly Conformable Adhesive

ThermalSeal® RT2™ Optically Transparent, Highly Conformable Adhesive, Polyester Sealing Film

Designed for use with standard 96-well plates where rows must be selectively protected or accessed. Available in a smaller cut for raised-rim and non-skirted PCR plates: ThermalSeal® RT2RR 

  • Certified DNase- & RNase- free
  • Ultra-high optical clarity
  • Minimizes evaporation and cross-contamination
  • Ideal for use with flat-top and hard-shell dual component (e.g. polycarbonate / polypropylene) PCR plates
  • Not recommended for removal after thermal cycling

Application

  • qPCR
Temperature Range
  • -40°C to +120°C

Helpful Tips

  • Selection of the proper film size will minimize evaporation
  • The film should not overhang or ride up the wall of a raised-rim plate
  • More complete coverage of skirted flat-top plates will reduce evaporation in the outer wells
  • End-tab removal before thermal cycling is highly recommended

Item#:
ASQPCRFILM
 
ThermalSeal RT2 Features
Specs ThermalSeal RT2
Sealing film selection guide by application
Sealing film selection guide by attributes

myView™ Compact UV Transilluminator

  • Uniform Illumination | Four 302 nm Bulbs
  • Adjustable UV Safety Cover | Easy Access to Gels
  • Large Scratch Resistant Viewing | 16.5 x 13.5 cm

The E3100 Compact UV Transilluminator has a surprisingly small footprint (just over 12 x 8 inches) yet provides industry leading performance. It will accommodate one or two mini gels on the 16.5 x 13.5 cm scratch resistant viewing surface.

The integrated UV blocking cover is made from a polymer that will not fluoresce in UV light. This allows clear viewing of DNA bands through the cover yet offers full UV blocking protection. Two friction hinges on the front edge of the cover allow for full adjustment. Position the cover horizontally for general viewing, tilt it at an angle for gel access and band cutting, or open fully to use the SmartDoc™ Imaging Enclosure (sold separately) for gel imaging with a smartphone.

The transilluminator’s four 6W bulbs evenly illuminate the viewing surface, and the 302 nm wavelength is ideal for Ethidium Bromide, SmartGlow™, SYBR Green®, Gel Green™ and many other common gel stains.  Spacing of the UV bulbs, coupled with a reflector system and special diffusing filter glass provides exceptional uniformity across the viewing surface. This allows for quantification of DNA and higher quality imaging when used with a camera system.



Specifications

Light source 4 x 6W UV Light Bulbs

4 x 6W UV Light Bulbs

Safety cover UV blocking polycarbonate

UV blocking polycarbonate

Exterior dimensions

10 x 8 x 2.75 in
25.4 x 20.32 x 6.99 cm

Electrical

12VDC (AC power adapter included)

Output wavelength

Peak at 302 nm

Viewing surface

6.5 x 5.3 in. | 16.5 x 13.5 cm

Weight

0.9 kg | 2 lbs

Warranty

2 years

Item#:
ASUVBENCHTRANS

Double sided water cooled vertical system, 10cm x 10cm


Reflection double sided water cooled vertical system, 10cm x 10cm with or without casting base, two pack each of plain and notched glass, one acrylic blocker plate, (2) 10-well 0.8mm thickness combs, (4) 0.8 mm spacers. Four year warranty.

System Description
 
Run either one or two gels simultaneously on these versatile separation systems. The 10x10cm size of the system also accomodates most pre-cast gels.

Complete double sided vertical system for running one or two gels, in a 10 x 10 cm format. Unique casting bases allows both casting and running of gels without unclamping from the upper buffer chamber, providing for safer and more convenient gel handling. In addition the 1010 device will accommodate most precast gel cassettes on the market.

Water cooling feature is included for protocols that require it. Connections are easily made by attaching standard tubing over the 3/8" barbed in/out ports provided on the device. Water may be provided by tap or other temperature controlled water source. Optional alumina backer plates may be used to facilitate cooling efficiency and uniformity, and are available in the accessories list below.

System Features
 
The Galileo Reflection™ 85-1010 complete system includes these features:

  • Heavy duty upper buffer chamber with cooling port connections.
  • Lower buffer chamber.
  • Interlock safety lid with attached leads.
  • Casting base.
  • Two-pack each of plain & notched glass plates.
  • One acrylic blocker plate (used when running only one gel).
  • Ttwo 10-well, 0.8mm thick combs
  • Four 0.8mm thick side spacers.
  • System Accessories
  • Choose amoung these accessories to customize each system for your specific needs.
  • Alumina cooling plate.
  • For use with cooling systems.
  • This plate can be sandwiched between the gel and the inner buffer chamber to improve overall temperature uniformity.
  • Casting base.
  • For quick, efficient and leak-proof casting.
  • Multiple gradient caster.
Item#:
ASDOUBSIDEWAT10C

Extra Wide Large Format Gel System, 23.5cmW x 40cm

 
Extra wide large format gel system, 23.5cmW x 40cmL gel size with buffer exchange ports, UVT gel tray with fluorescent ruler and 12 tray slots, (2) 25 teeth combs, 1.5 mm thickness and (2) 50 teeth combs, 1.5 mm thickness.
System Description

For long run lengths, maximum resolution, and really large sample capacity, look no further than the Galileo 2340 device. This unit also features the Galileo ExpressCast system for quick tape-less gel pouring.

The 2340 is our maximum capacity system. With a maximum run length of 25cm, the unit can provide very high resolution of complex analytes. Or, fitted with maximum capacity combs in all of the tray's 12 comb slots, the unit can handle up to 600 samples. The 2340 features the Galileo ExpressCast gel casting system for quick, tape-free gel casting and includes a built-in bubble level and leveling screws for convenient level adjustment. You may choose from a wide variety of comb options to customize your order.
System Features

The Galileo 2340 complete system includes these features:

  •     Heavy duty lower buffer chamber
  •     Interlock safety lid with attached leads
  •     End gated 23cm wide x 40cm long ExpressCast™ UV-transparent gel tray with built-in fluorescent ruler and 12 comb slots.
  •     Two 1.5mm x 25 tooth combs
  •     Two 1.5mm x 50 tooth combs
Item#:
ASEXTRAWIDELGFOR23
White Light, 21x26cm, Single

Uniform white light diffusion for viewing negative and film positives. X-ray film, autoradiographs, microtiter plates, and stained gels can be viewed on the white light transmitting glass surface. This surface, permanently double-safety sealed into the unit for durability, provides a scratch and chemical resistant work area which can be cleaned easily. Durable transilluminator housing is scratch resistant, epoxy powder paint finish for easy cleaning. 2 year warranty

ModelOrder NoDescriptionFilter SizeDimensions
TW-26 95-0208-01White Light Transilluminator, 115V 21 x 26cm9.5"D x 13.25"W x 4.25"H
TW-4395-0214-01White Light Transilluminator, 115V 36 x 43cm19.13"D x 16"W x 4.25"H

Safety Certifications: ETL, ETL/c on all 115V models.
Item#:
95-0208-01
Your Price:
1149.86
Each

Extra Wide Format Gel System, 23.5cmW x 25cmL


 ExpressCast extra wide format gel gystem for 23.5cmW x 25cmL gel size. Includes UVT gel tray with fluorescent rulers and 14 comb slots, (2) 25 tooth combs, 1.5 mm thickness, and (2) 50 tooth combs, 1.5 mm thickness and gasketed end-gates.
System Description

Need long run lengths for maximum resolution, and large sample capacity too? Look at the features of the Galileo 2325 unit and we think you will find just what you need.

The 2325 is a full sized large capacity system. With a maximum run length of 25cm, the unit can provide high resolution of complex analytes. Or, fitted for maximum capacity, the unit can handle up to 500 samples. The 2325 features the Galileo ExpressCast gel casting system for quick, tape-free gel casting and includes a built-in bubble level and leveling screws for convenient level adjustment. You may choose from a wide variety of comb options to customize your order.
System Features

The Galileo 2325 complete system includes these features:

  •     Heavy duty lower buffer chamber
  •     Interlock safety lid with attached leads
  •     End gated 23cm wide x 25cm long ExpressCast™ UV-transparent gel tray with built-in fluorescent ruler and 14 comb slots.
  •     Two 1.5mm x 25 tooth combs
  •     Two 1.5mm x 50 tooth combs
Item#:
ASEXTRAWIDEFOR23
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