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Multi-Therm™ Incubated Vortexer

Item #: ASVORTEX5 

2-year Warranty

Bullseye

This brand is being discontinued, please contact us for other options.

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Bullseye PREMIUM GC Rich Optimized DNA Polymerases and Kits

Bullseye offers a product series specifically developed for the amplification of GC-rich DNA sequences. The Bullseye HS DNA Polymerase combined with GC Buffer I and GC Buffer II promote excellent amplification results with targets of varying degrees of GC content. Hot Start DNA Polymerase is a modified form of Bullseye Taq DNA Polymerase. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during setup and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. Once the reaction reaches optimal activating temperature, the chemical moiety is cleaved during a 10-15 minutes heat activation step, releasing the active Hot Start DNA Polymerase into the reaction. The GC Buffers in combination with Hot Start DNA Polymerase and the heat activation step result in a high success rate in amplification of DNA targets with high GC content.

Key Features

  • Amplification of multiple DNA targets with high GC content
  • High specificity, sensitivity and product yield
  • Diminished formation of non-specific product
  • Detection of low copy number targets

Kit Components

  • HS DNA Polymerase in Storage Buffer
  • 5 U/ml Hot Start DNA Polymerase, 20 mM Tris-HCl pH 8.9, 100
  • mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Tween 20,
  • 0.5% NP40, 50% glycerol.
  • 4x GC Buffer I
  • Optimized buffer components, 6 mM MgCl
  • 4x GC Buffer II
  • Optimized buffer components, 6 mM MgCl
  • MgCl
  • 25 mM MgCl in PCR grade water

PNPCRREAG

Magnesium
Mg
Dye
No Dye
Enzymes
Premium
Pkg
Indiv
Type
Hot-Start GC Rich
High Fidelity Polymerase
Item#:
ASACCREAG4
 
High Fidelity Hot Start M
Item#:
ASACCURISHIFIDHS
 
SKU Number Speed Capacity Price Quantity Add to Cart
MTH-1500 Adjustable Speed Shaker 8 x 11 in, Heating
2498.92
Each
MTHC-1500 Adjustable Speed Shaker 8 x 11 in, Heating & Cooling
3363.71
Each
MTB-500 Block 50 mL x 6 tubes
519.10
Each
MTB-5MT Block 5 mL x 15 conical tubes
519.10
Each
MTB-02 Block 0.2 mL x 96 tubes or 1 PCR plate
437.85
Each
MTB-05 Block 0.5 mL x 54 tubes
368.50
Each
MTB-12 Block 12 mm x 24 tubes
519.10
Each
MTB-15 Block 1.5 mL x 35 tubes
368.50
Each
MTB-20 Block 2 mL x 35 tubes
368.50
Each
MTB-1232 Block 2 mL x 24 AutoSample HPLC tubes
519.10
Each
MTB-150 Block 15 mL x 12 tubes
519.10
Each
MTB-MP Block 1 x microplate
519.10
Each
MTB-DWMP Block 1 x deepwell Plate
519.10
Each
MTB-CU Block Custom
1037.92
Each
MTB-CMB Combo Block 0.5 mL x 15 and 1.5 mL x 20 tubes
368.50
Each
MBT-WB Block Multi-purpose for water, beads, etc
519.10
Each
MBT-025 Block 250 uL vial insert x 48
407.83
Each
Multi-Therm Incubated Sha
Multi-Therm™ Incubated Vortexer
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