TheWell Bioscience VitroGel® ORGANOID

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VitroGel® ORGANOID
 
ready-to-use, xeno-free (animal origin-free) hydrogel system for organoid culture

• Xeno-free hydrogel for organoid formation
• Supports a wide range of organoids from patient-derived samples, stem cells, tissues, co-culture and PDX resources
• Scalable protocol for lab automation
• Easy 20-minute cell harvesting (enzyme-free)
• VitroGel® ORGANOID (1-4) are xeno-free (animal origin-free) hydrogels that support the growth of patient-derived organoids or organoids developed from pluripotent stem cells (PSCs), co-culture, and PDX model.

VitroGel® ORGANOID hydrogels are ready to use at room temperature and have a neutral pH, transparent, permeable, and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. VitroGel® ORGANOID hydrogels are good for both 3D cell culture and 2D hydrogel coating applications.

The VitroGel® ORGANOID hydrogels can work in conjunction with VitroGel® STEM (a xeno-free hydrogel for 3D static suspension cultures and scale-up of human pluripotent stem cells) by transferring stem cell spheroids cultured in VitroGel® STEM to VitroGel® ORGANOID hydrogels for organoid differentiation. See Xeno-free 3D Organoid Workflow B.  Key growth factors and molecules can be directly mixed with the hydrogel matrix or by adding on the top of the hydrogel. Organoids cultured in this system can be easily harvested out with our VitroGel® Cell Recovery Solution.  VitroGel® ORGANOID hydrogels provide a well-defined 3D microenvironment for the future of personalized medicine.

Which organoid hydrogel to choose

We still strongly suggest starting with the VitroGel® ORGANOID Discovery Kit (Item # VHM04-K) to make a quick screening to find the hydrogel version that is optimal for the organoid conditions.

The kit includes all four types of organoid hydrogels:

VitroGel® ORGANOID-1, VitroGel® ORGANOID-2, VitroGel® ORGANOID-3, VitroGel® ORGANOID-4)

Each of the four types has various bio-functional ligands, mechanical strengths, and degradability to fulfill the needs of different organoid culture conditions.

Because there are a wide range of organoid cell resources (from stem cells, patient-derived tissue, co-culture, to PDX), it is hard to tell which organoid hydrogel would best perform the researcher’s experiment. Therefore, the Discovery Kit helps researchers to screen the four different formulations to determine the best organoid hydrogel version for the organoid conditions.

From our findings, versions 1, 2, or 3 are suitable for gastric organoids. Versions 1 or 3 are suitable for lung organoids. Versions 2 or 3 are suitable for brain organoids, and versions 3 or 4 are suitable for cancer organoids.

Specifications

TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.   

TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.

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