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ActionsTheWell Bioscience VitroGel® Angiogenesis Assay Kit
For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.
VitroGel® Angiogenesis Assay Kit
ready-to-use, hydrogel system for 2D gel coating and 3D
culture of angiogenesis tube formation, invasion, and animal injection.
- Hydrogel - Hydrogel Only: VitroGel® AAK (2 mL)
- Type 1 - Full Kit (Hydrogel+Supplements): VitroGel AAK (2 mL), AAK Supplement 1 (500 µL), AAK Supplement 2 (500 µL)
- Type 2 - Full Kit (Hydrogel+Supplements): VitroGel AAK (2 mL), AAK Supplement 1 (2 x 500 µL)
- Type 3 - Full Kit (Hydrogel+Supplements): VitroGel AAK (2 mL), AAK Supplement 2 (2 x 500 µL)
VitroGel Angiogenesis Assay Kit
- Xeno-free hydrogel system supporting angiogenesis process
- Multiple applications in one kit: Tube formation, invasion, and animal injection
- Control the hydrogel properties: Add your own growth factors and compare with positive control
- Easy, efficient, and enzyme-free cell harvesting in 20 minutes
VitroGel® Angiogenesis Assay Kit is a revolutionary tool for researchers to study the effect of both hydrogel properties and culture medium on angiogenesis process. The kit can be used to study the angiogenesis tube formation and invasion on both 2D hydrogel coating method and 3D cell culture method. The VitroGel® system is also good for animal injection for in vivo study.
Angiogenesis is a highly regulated process that involves the growth of new blood vessels from the existing vasculature. This process plays an important role in both normal developmental processes and numerous pathologies, including wound healing, tumor growth, and metastasis to inflammation and ocular disease.
Traditional angiogenesis assay highly relies on natural extracellular matrix (natural ECM), which has non-adjustable hydrogel compositions and properties. Therefore, our understanding of the angiogenesis process is limited by studying the molecular cues such as growth factors and inhibitors in culture medium only. There is a lack of knowledge on how the properties of hydrogel affect the angiogenesis process.
There are two versions of VitroGel® Angiogenesis Assay Kits:
- VitroGel® Angiogenesis Assay Kit (Ready-To-Use): Ready-to-use with a fixed hydrogel mechanical strength to support the angiogenesis assay with adjustable supplements
- VitroGel® Angiogenesis Assay HC Kit (Cat No. TWG011-K): Assay kit with a tunable high concentration hydrogel to allow full control of the hydrogel’s mechanical strength with adjustable supplements.
Depending on the kit type, the ready-to-use VitroGel®
Angiogenesis Assay Kits can contain the following components:
- VitroGel® AAK, a xeno-free ready-to-use hydrogel.
- AAK Supplement 1, a hydrogel supplement without vascular endothelial growth factors (VEGFs) for cell attachment and growth.
- AAK Supplement 2, a hydrogel supplement with VEGFs to promote tube formation and as a positive control.
The VitroGel® AAK hydrogel is room temperature stable and
can be directly mixed with each supplement at 2:1 (v/v) ratio for hydrogel
formation. Researchers can adjust the
hydrogel’s molecular cues by adding the growth factors/inhibitors directly to
the supplements before mixing with the VitroGel® AAK hydrogel. Cells cultured in this system can be
harvested further easily with the VitroGel® Cell Recovery Solution.
Contents and Specifications
TYPE
1 Kit Contents |
VitroGel® AAK
(2 mL) |
AAK
Supplement 1 (1 x 500 µL) |
|
AAK
Supplement 2 (1 x 500 µL) |
|
TYPE
2 Kit Contents |
VitroGel® AAK
(2 mL) |
AAK
Supplement 1 (2 x 500 µL) |
|
TYPE
3 Kit Contents |
VitroGel® AAK
(2 mL) |
AAK
Supplement 2 (2 x 500 µL) |
|
Formulation |
Ready-to-use,
xeno-free functional hydrogel |
AAK
Supplement 1: Without VEGFs |
|
AAK
Supplement 2: With VEGFs |
|
Use |
Angiogenesis
Assay, tube formation, invasion, animal injection |
Biocompatibility |
Biocompatible,
safe for animal studies |
Injection |
Injectable
hydrogel for in vivo studies and lab automation |
Cell
Harvesting |
Easy
cell recovery using VitroGel® Cell Recovery Solution |
pH |
Neutral |
Shipping |
Supplements
require dry ice shipment. |
Storage |
VitroGel® AAK
hydrogel: store at 2-8°C |
AAK
Supplement 1 and AAK Supplement 2: store at -20°C |
|
Number
of Uses |
60
uses at 50 µL per well |
TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.
TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.