PR1MA™ CRISPR/Cas9 Nucleases

The RNA-guided endonuclease Cas9, associated with Type II CRISPR/Cas systems, site-specifically digests DNA using a single guide RNA (sgRNA) which it binds to direct it to the complementary sequence. MIDSCI™ offers two traditional versions of Streptococcus pyogenes Cas9 nuclease: CRISPR/Cas9, ideal for in vitro DNA digestion, and CRISPR/Cas9 NLS, for in vivo nuclear localization.

• Optimal temperature: 37°C
• Heat inactivation: 65°C for 20 minutes
• Storage temperature: -20°C

Buffer composition

• 50% glycerol
• 50 mM Tris-HCl
• 50 mM KCl
• 1 mM DTT
• 0.1  mM EDTA
• pH = 7.5 

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.