DEPC Treated Water

The Fast Ion Plasmid Maxi Kit (Endotoxin Free) uses pre-packed anion exchange resin columns to purify plasmid DNA from 100-250 ml of bacterial cultures. Modified alkaline lysis method (1) and RNase treatment are used for obtaining clear cell lysate with minimal genomic DNA and RNA contaminants. Once the plasmid DNA has been bound to the column, the contaminants can be washed off using the wash buffer. Finally, the purified plasmid DNA is eluted by a high salt buffer and precipitated with isopropanol for desalting. The entire procedure can be completed in 120 minutes and the obtained high purity plasmid DNA is suitable for transfection, sequencing reactions, PCR and in-vitro transcription.