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PREMIUM Bullseye GC Rich Optimized DNA Polymerases and Kits

Discontinued, Contact us for more options!
Item #: ASPCRREAG7 
ASPCRREAG7

This brand is being discontinued, please contact us for other options.

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Visit our PR1MA Polymerase page for more options! 


General Description
Bullseye offers a product series specifically developed for the amplification of GC-rich DNA sequences. The Bullseye HS DNA Polymerase combined with GC Buffer I and GC Buffer II promote excellent amplification results with targets of varying degrees of GC content. Hot Start DNA Polymerase is a modified form of Bullseye Taq DNA Polymerase. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during setup and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. Once the reaction reaches optimal activating temperature, the chemical moiety is cleaved during a 10-15 minutes heat activation step, releasing the active Hot Start DNA Polymerase into the reaction. The GC Buffers in combination with Hot Start DNA Polymerase and the heat activation step result in a high success rate in amplification of DNA targets with high GC content.

Key Features

  • Amplification of multiple DNA targets with high GC content
  • High specificity, sensitivity and product yield
  • Diminished formation of non-specific product
  • Detection of low copy number targets

    Kit Components:
    HS DNA Polymerase in Storage Buffer
    5 U/ml Hot Start DNA Polymerase, 20 mM Tris-HCl pH 8.9, 100
    mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% TweenÒ 20,
    0.5% NP40, 50% glycerol.
    4x GC Buffer I
    Optimized buffer components, 6 mM MgCl
    4x GC Buffer II
    Optimized buffer components, 6 mM MgCl
    MgCl
    25 mM MgCl in PCR grade water

PNPCRREAG

Magnesium
Mg
Dye
No Dye
Enzymes
Premium
Pkg
Indiv
Type
Hot-Start GC Rich
 
 
SKU Number Vol Magnesium Dye Enzymes Pkg Type Price Quantity Add to Cart
BE227103 500 Units Mg No Dye Premium Indiv Hot-Start GC Rich
168.89
Each
  Item is no longer available.  
BE227104 1,000 Units Mg No Dye Premium Indiv Hot-Start GC Rich
307.63
Each
  Item is no longer available.  
BE227106 2500 units Mg No Dye Premium Indiv Hot-Start GC Rich
746.02
Each
  Item is no longer available.  
ASPCRREAG7
PREMIUM Bullseye GC Rich Optimized DNA Polymerases and Kits
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