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Home > Supplies > Bottles > Culture Bottles > Hybridization Bottles

Hybridization Bottles

Item #: ASHYRBIDBOTT

Sample: up to 25 mg of tissue, up to 25 mg of paraffin-embedded tissue
Yield: 5-30 µg
Format: Spin column
Operation time: Within 20 minutes

Introduction
The Total RNA Mini Kit (Tissue) was designed specifically for purifying total RNA from a variety of animal and paraffin-embedded tissue. Tissue samples can be efficiently homogenized in a microcentrifuge tube using the provided micropestle. Detergents and chaotropic salt are used to lyse cells and inactivate RNase and optional DNase treatments can be followed to remove unwanted DNA residue. RNA in the chaotropic salt is bound by the glass fiber matrix of the spin column (1). Once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-Free Water, and is ready for use in RT-PCR, Northern Blotting, Primer Extension and cDNA Library Construction. Phenol extraction or alcohol precipitation is not required.

Quality Control
The quality of the Total RNA Mini Kit (Tissue) is tested on a lot-to-lot basis by isolating total RNA from a 25 mg animal tissue sample. The purified RNA is quantified with a spectrophotometer and checked by electrophoresis.

Kit	IB47300	IB47301	IB47302	IB47303
RB Buffer	2 mL	30 mL	60 mL	130 mL
W1 Buffer	2 mL	30 mL	50 mL	130 mL
Wash Buffer* (Add EtOH)	1 mL (4 mL)	12.5 mL (50 mL)	25 mL (100 mL)	50 mL x 2 (200 mL x 2)
RNase-free H2O	1 mL	6 mL	15 mL	30 mL
RB Column	4 pcs	50 pcs	100 pcs	300 pcs
Filter Column	4 pcs	50 pcs	100 pcs	300 pcs
2 mL Collection Tubes	8 pcs	100 pcs	200 pcs	600 pcs
Micropestle	4 pcs	50 pcs	100 pcs	300 pcs

*Add absolute ethanol (see the bottle label for volume) to the Wash Buffer prior to initial use.

Caution
RB Buffer contains chaotropic salt which is a harmful irritant. During operation, always wear a lab coat, disposable gloves, protective goggles, and (anti-fog) procedure mask.

PNRNADNA

Isolation type

RNA

Source Cell

Lyse Cell

AlphaPette™ by PR1MA™ Pipettors

PR1MA™ Low Retention Graduated Barrier Pipette Tips

SKU Number	Type	Operating Temp	Vol	Price	Quantity	Add to Cart
H-3315	Bottle	N/A	33 x 150mm	64.5700 64.57 Each
H-3510	Bottle	N/A	35 x100m	62.3900 62.39 Each
H-3510H	Bottle	N/A	35 x100m	49.4700 49.47 Each
H-3525	Bottle	N/A	35 x 250mm	85.3800 85.38 Each
H-3525H	Bottle	N/A	35 x 250mm	64.2800 64.28 Each
H-3575	Bottle	N/A	35 x 75mm	60.7000 60.70 Each
H-3575H	Bottle	N/A	35 x 75mm	48.7300 48.73 Each
H-9082	Bottle	N/A	35 x 300mm	89.2300 89.23 Each
H-9082H	Bottle	N/A	35 x 300mm	66.8900 66.89 Each
H-9084	Bottle	N/A	35 x 150mm	71.7000 71.70 Each
H-9084H	Bottle	N/A	35 x 150mm	52.2200 52.22 Each
H-9084-NC	Bottle	N/A	35 x 150mm	43.7800 43.78 Each
QHB-BOOR	Blue Cap	N/A	N/A	11.7300 11.73 Each
QHB-RO	Red Cap w/Line	N/A	N/A	18.9200 18.92 Each
QHB-ROOR	Red Cap	N/A	N/A	20.5600 20.56 Each