Ambient Universal Centrifuge from Hettich

Accuris qMAX™ One Step Kits allow for highly sensitive real time RT-qPCR assays to be performed directly from RNA templates.  Workflows are simplified with optimized formulations of ready-to-use 2X qPCR master mix and 20X reverse transcriptase.

Optimized buffer includes powerful RNase inhibitors and an extremely thermostable MMLV-derived reverse transcriptase enables robust first strand cDNA synthesis.  Accuris Hot Start Taq uses an antibody mediated hot start mechanism allowing for sample preparation at room temperature.  Only after an initial incubation at 95C will the Taq become active, so non-specific amplification is greatly reduced.  An inert blue dye is included in the Taq master mix to help simplify pipetting and reduce errors.

Three versions of our One Step qPCR kits are available:

qMAX™ Green One Step kits incorporate our proprietary intercalating dye which exhibits higher fluorescent and lower PCR inhibition than other popular dyes such as SYBR.

qMAX™ Probe One Step kits are optimized for use with popular TaqMan™, Scorpions, and molecular beacon probes.

All Accuris One-Step Kits are compatible with standard and fast cycling protocols and provide increased sensitivity, speed and reproducibility for a broad range of samples and targets.  The polymerase mix is available with different levels of ROX reference dye for compatibility with all qPCR instruments.

Bulk Packaging: 

To accommodate the requirements of high throughput users, Accuris is now offering their full line of qPCR and RT-qPCR reagents in bulk packaging at highly discounted prices. Bottle sizes of 25ml (2500 reactions) and 50ml (5000 reactions) are available for qMAX SYBR Green and qMAX Probe mixes. One Step RT-qPCR kits are also available in bulk format for 2500 and 5000 reactions and are ideal for labs performing pathogen detection.

All PCR reagents ship in protective coolers with frozen gel packs.