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Low Retention Filter Pipette Tips

Item #: ASCELFILTIPS 
RT (reverse transcriptase


This brand is being discontinued and will only be available while supplies last.


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Bullseye Taq Plus Master Mix 

  • Pre-optimized ready-to-use PCR reagents
  • Less pipetting to avoid contamination
  • Quick and easy to set up
  • Direct loading for electrophoresis
  • Reproducible results

Users only need to add templates and primers, and water if needed. Extra 25 mM magnesium solution is also provided for additional fine adjustments. The Red Mixes contain a non-hazardous inert red dye. The purple mixes contain a non-hazardous inert purple dye. Completed PCR reactions utilizing the D124-R (red dye) or D124-P (purple dye) can be directly loaded into a well of agarose gel or other gels, for electrophoresis. No extra loading buffer is needed.

Storage: 4°C for up to one month, or -20°C for long term storage.

Magnesium Chloride: In general, 1.5 mM MgCl2 is recommended; this may vary with different conditions and primer sets. Some primers/templates may require adjustments for MgCl2 concentration, which can be achieved as shown below:

Final MgCl2 concentration1.5 mM2.0 mM2.5 mM
Additional 25 mM MgCl2 per 50 uL reaction0.0 uL1.0 uL2.0 uL

Directions for use: For a 50 uL reaction, use 25 uL of the Taq Master Mix, add template, primers, and water to a final volume of 50 uL. Cycling conditions vary for different templates and primers. To start with, try 30 cycles as follows: denature at 94°C for 30 seconds, anneal around 55°C for 30 seconds, and extend at 72°C for 1 minute / kb. After the PCR cycles, extend at 72°C for another five minutes to complete the PCR. Then store the reaction at 4°C.

1x Composition: 10 mM KCl, 20 mM Tris HCl (pH 9.0), 16 mM (NH4)2SO4, 0.1% Triton X-100, 1.5 mM MgCl2, 200 mM dNTPs, 2.5 units / 25 uL of Taq DNA polymerase, (optional: trace amount of red or purple dye) and enzyme stabilizers.
 

PNPCRREAG

Magnesium
Mg
Enzymes
Standard
Pkg
Mix
Type
Standard
Item#:
ASACCREAG1
 
 
SKU Number Tip Fit Lid Type Vol Sterile Qty Price Quantity Add to Cart
229015 Universal Racked w/ Hinged Lids 10 uL Sterile 10 racks of 96 tips
96.00
Each
229016 Universal Racked w/ Hinged Lids 10 uL XL Sterile 10 racks of 96 tips
97.00
Each
229017 Universal Racked w/ Hinged Lids 20 uL Sterile 10 racks of 96 tips
102.00
Each
229018 Universal Racked w/ Hinged Lids 100 uL Sterile 10 racks of 96 tips
102.00
Each
229019 Universal Racked w/ Hinged Lids 200 uL Sterile 10 racks of 96 tips
102.00
Each
229020 Universal Racked w/ Hinged Lids 300 uL Sterile 10 racks of 96 tips
106.00
Each
229021 Universal Racked w/ Hinged Lids 1,000 uL Sterile 10 racks of 96 tips
120.00
Each
229022 Universal Racked w/ Hinged Lids 1,000 u XL Sterile 10 racks of 96 tips
130.00
Each
Low Retention Filter Tips
Low Retention Filter Pipette Tips
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