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Labcon SuperClear® 15 and 50 mL Centrifuge Tubes

Item #: ABLCSSCLR1550 
RT (reverse transcriptase


This brand is being discontinued and will only be available while supplies last.


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Bullseye Taq Plus Master Mix 

  • Pre-optimized ready-to-use PCR reagents
  • Less pipetting to avoid contamination
  • Quick and easy to set up
  • Direct loading for electrophoresis
  • Reproducible results

Users only need to add templates and primers, and water if needed. Extra 25 mM magnesium solution is also provided for additional fine adjustments. The Red Mixes contain a non-hazardous inert red dye. The purple mixes contain a non-hazardous inert purple dye. Completed PCR reactions utilizing the D124-R (red dye) or D124-P (purple dye) can be directly loaded into a well of agarose gel or other gels, for electrophoresis. No extra loading buffer is needed.

Storage: 4°C for up to one month, or -20°C for long term storage.

Magnesium Chloride: In general, 1.5 mM MgCl2 is recommended; this may vary with different conditions and primer sets. Some primers/templates may require adjustments for MgCl2 concentration, which can be achieved as shown below:

Final MgCl2 concentration1.5 mM2.0 mM2.5 mM
Additional 25 mM MgCl2 per 50 uL reaction0.0 uL1.0 uL2.0 uL

Directions for use: For a 50 uL reaction, use 25 uL of the Taq Master Mix, add template, primers, and water to a final volume of 50 uL. Cycling conditions vary for different templates and primers. To start with, try 30 cycles as follows: denature at 94°C for 30 seconds, anneal around 55°C for 30 seconds, and extend at 72°C for 1 minute / kb. After the PCR cycles, extend at 72°C for another five minutes to complete the PCR. Then store the reaction at 4°C.

1x Composition: 10 mM KCl, 20 mM Tris HCl (pH 9.0), 16 mM (NH4)2SO4, 0.1% Triton X-100, 1.5 mM MgCl2, 200 mM dNTPs, 2.5 units / 25 uL of Taq DNA polymerase, (optional: trace amount of red or purple dye) and enzyme stabilizers.
 

PNPCRREAG

Magnesium
Mg
Enzymes
Standard
Pkg
Mix
Type
Standard
Item#:
ASACCREAG1
 
 
SKU Number Bottom Style Cap Style Color Pkg Qty Temp Range Vol Price Quantity Add to Cart
3131-165-008-9 Conical Flat Caps Clear Individually Wrapped - Sterile 100 tubes -80C to 122C 15 mL
305.91
Each
3131-325-008-9 Conical Flat Caps Clear IntegraPack - Sterile 10 packs of 50 tubes -80C to 122C 15 mL
316.11
Each
3131-340-008-9 Conical Flat Caps Clear Bagged 10 bags of 50 tubes -80C to 122C 15 mL
176.07
Each
3131-345-008-9 Conical Flat Caps Clear Bagged - Sterile 10 bags of 50 tubes -80C to 122C 15 mL
206.95
Each
3132-340-008-9 Conical Plug-Style Caps Clear Bagged 10 bags of 50 tubes -80C to 122C 15 mL
213.27
Each
3132-345-008-9 Conical Plug-Style Caps Clear Bagged - Sterile 10 bags of 50 tubes -80C to 122C 15 mL
225.52
Each
3141-330-018-9 Conical Flat Caps Clear Cardboard Fiber Rack 20 racks of 25 tubes -80C to 122C 15 mL
214.86
Each
3141-335-018-9 Conical Flat Caps Clear Cardboard Fiber Rack - Sterile 20 racks of 25 tubes -80C to 122C 15 mL
287.32
Each
3141-335-028-9 Conical Flat Caps Clear Plastic Rack - Sterile 20 racks of 25 tubes -80C to 122C 15 mL
271.74
Each
3141-870-008-9 Conical Flat Caps Clear Bulk (Tubes & Caps Separate Bags) 500 tubes & 500 caps -80C to 122C 15 mL
191.65
Each
3142-330-018-9 Conical Plug-Style Caps Clear Cardboard Fiber Rack 20 racks of 25 tubes -80C to 122C 15 mL
209.96
Each
3142-335-018-9 Conical Plug-Style Caps Clear Cardboard Fiber Rack - Sterile 20 racks of 25 tubes -80C to 122C 15 mL
240.84
Each
3142-870-008-9 Conical Plug-Style Caps Clear Bulk (Tubes & Caps Separate Bags) 500 tubes & 500 caps -80C to 122C 15 mL
191.65
Each
3181-165-008-9 Conical Flat Caps Clear Individually Wrapped - Sterile 100 tubes -80C to 122C 50 mL
373.67
Each
3181-325-008-9 Conical Flat Caps Clear IntegraPack - Sterile 10 packs of 50 tubes -80C to 122C 50 mL
377.87
Each
3181-340-008-9 Conical Flat Caps Clear Bagged 10 bags of 50 tubes -80C to 122C 50 mL
195.78
Each
3181-345-008-9 Conical Flat Caps Clear Bagged - Sterile 10 bags of 50 tubes -80C to 122C 50 mL
228.53
Each
3182-340-008-9 Conical Plug-Style Caps Clear Bagged 10 bags of 50 tubes -80C to 122C 50 mL
222.53
Each
3182-345-008-9 Conical Plug-Style Caps Clear Bagged - Sterile 10 bags of 50 tubes -80C to 122C 50 mL
231.82
Each
3191-330-018-9 Conical Flat Caps Clear Cardboard Fiber Rack 20 racks of 25 tubes -80C to 122C 50 mL
261.12
Each
3191-335-018-9 Conical Flat Caps Clear Cardboard Fiber Rack - Sterile 20 racks of 25 tubes -80C to 122C 50 mL
342.77
Each
3191-335-028-9 Conical Flat Caps Clear Plastic Rack - Sterile 20 racks of 25 tubes -80C to 122C 50 mL
305.91
Each
3191-870-008-9 Conical Flat Caps Clear Bulk (Tubes & Caps Separate Bags) 500 tubes & 500 caps -80C to 122C 50 mL
191.65
Each
3192-330-018-9 Conical Plug-Style Caps Clear Cardboard Fiber Rack 20 racks of 25 tubes -80C to 122C 50 mL
278.02
Each
3192-335-018-9 Conical Plug-Style Caps Clear Cardboard Fiber Rack - Sterile 20 racks of 25 tubes -80C to 122C 50 mL
290.33
Each
3192-870-008-9 Conical Plug-Style Caps Clear Bulk (Tubes & Caps Separate Bags) 500 tubes & 500 caps -80C to 122C 50 mL
209.96
Each
LabCon SuperClear Centrif
Labcon SuperClear® 15 and 50 mL Centrifuge Tubes
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