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Azure Biosystems Cielo qPCR System
For a limited time, take advantage of this special promo price for
AIQ060 Azure Cielo 6 Real-Time PCR 96-well RT PCR instrument!
Promo valid through June 30, 2023
Not to be combined with any offers or discounts. No institutional pricing. All terms apply.
AIQ060 Azure Cielo 6 Real-Time PCR 96-well RT PCR instrument!
Promo valid through June 30, 2023
Not to be combined with any offers or discounts. No institutional pricing. All terms apply.
Azure Cielo 3 & 6
The Azure Cielo Real-Time PCR system provide accuracy and sensitivity needed for research, with intuitive touch screen software.
Product Features
- Flexibility: Engineered for a wide variety of qPCR applications, with high sensitivity and wide dynamic range.
- Intelligent workflow: The user interface is a simple touch screen assay setup. Protocols can be saved to make assay set up easy and reproducible.
- Reliability: Cielo's optics and thermal block are designed to deliver the same uniformity and reproducibility over at least 1,000 qPCR experiments, giving you confidence in the reliability of the instrument.
- Connectivity: An external PC is not required to run the instrument.
- Data Transfer: This can be performed via Ethernet, Wi-Fi or USB.
Operation - A standalone qPCR system with 10.3” touchscreen interface
Optics - 3 or 6 dye channel filters with LED based excitation for multiplexing
Speed - Fiber optic detection system scans 16 wells simultaneously
Durability - Tested reliability for over 1000 continuous runs
Connectivity - Easily export data via Wi-Fi, ethernet, USB or LAN
Capacity - 96-well format for semi-skirted or un-skirted plates and tubes
Thermal Performance - Marlow Peltier based block with 12 temperature gradient zones, ramp rates up to 6.0°C/sec
Chemistry - Supports reaction volumes between 10 µl–150 µl, open source chemistry, including FAST ~20 minute run-times
Sensitivity - Detect 2-fold differences in target quantities in singleplex reactions and up to 1 copy of amplified DNA, 10 logs of dynamic range
Competitor Q uses a single light source to excite all samples.
Differences in light path length lead to differences in excitation, which requires normalization. Though Competitor Q has said that they do not require normalization dyes, this is not reflected in the data as the data CV values for replicates is significantly higher.
Why doesn’t the Cielo require ROX dye?
The Cielo does not require ROX dye because it uses in-well detection technology. The light path for excitation and reading emission are collimated directly into the well. As such, the light path length is always the same for each well, so there is no need to normalize for varying light path length. The data from the Cielo is consistently uniform and reproducible.
Azure
Cielo 3 AIQ030 |
Azure
Cielo 6 AIQ060 |
|
Dyes/Probes |
||
SYBR™ Green, EvaGreen™, FAM™ |
x |
x |
VIC®, HEX™, JOE™, CAL Fluor® 540, CAL Fluor® Orange 560 |
x |
x |
TAMRA™ |
|
x |
ROX™, TEX®615, CAL Fluor® Red 610 |
|
x |
Cy®5, Quasar®670, Liz®, Mustang Purple® |
x |
x |
Cy®5.5 |
|
x |
Other
dyes/probes are possible depending on the system configuration. |
