PR1MA™  SOB Broth, Capsules

CAS Number: 91079-40-2; 8013-01-2; 7647-14-5; 7487-88-9; 7447-40-7
Solubility: Water
Storage Temperature: Room Temperature

SOB Broth is recommended for use in cultivation of recombinant strains of E. Coli. It is a nutritionally rich growth medium for use in the preparation and transformation of competent cells.


Add two capsules to one liter of distilled water. Heat and stir gently to completely dissolve medium. Sterilize by autoclaving at 121°C for 15 minutes.


Final Solution contains:

• Casein Enzymic Hydrolysate: 20 g/L
• Yeast Extract: 5 g/L
• Sodium Chloride: 0.5 g/L
• Magnesium Sulfate: 2.4 g/L
• Potassium Chloride: 0.186 g/L

Research or further manufacturing use only, not for food or drug use.

• Appearance: Capsules
• Appearance of Solution: Prepared medium is light to medium amber and clear
• pH @25 °C: 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent