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Accuris™ MR9600 SmartReader™ 96 Microplate Absorbance Reader

Item #: ASACURISPLTREAD 

IBI Total RNA Purificatio

IBI Tri-Isolate is a phenol and guanidine isothiocyanate plus spin column system for convenient purification of high-quality total RNA from a variety of samples. Initially, samples are homogenized in IBI Isolate without chloroform phase separation or isopropanol RNA precipitation. Following sample homogenization, simply bind, wash and elute the high-quality, total RNA in RNase-free Water and use in a variety of sensitive downstream applications.

Quality Control
IBI Tri-Isolate is tested on a lot-to-lot basis. An Escherichia coli (1 x 109) culture (OD600=1.3, 1 mL) is harvested by centrifugation at 16,000 x g for 2 minutes, followed by IBI Isolate homogenization. RNA is then purified using a spin column procedure. 10 µL from a 50 µL eluate of purified RNA is analyzed by electrophoresis on a 0.8% agarose gel.

Advantages

  • Purify total RNA within 15 minutes without chloroform phase separation or isopropanol RNA precipitation
  • Up to: 200 µL (blood, buffy coat, serum, plasma), 5 x 106 (cultured cells), 10-50 mg (tissue), 1 x 109 (bacteria cells)
  • A cost effective phenol, guanidine isothiocyanate solution plus spin column system
  • High quality RNA: A260/A280 >1.8, A260/A230 >1.8
  • Applications: cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays,
  • Northern Blotting

Caution
IBI Isolate contains phenol and guanidine isothiocyanate. During operation, always work in a fume hood, always wear a lab coat, disposable gloves, protective goggles and (anti-fog) procedure mask. Disposable/non-disposable glassware, plasticware and automatic pipettes should be sterile (RNase-free) and used only for RNA procedures.

Components and Storage

Item

Volume

Product

Shipping

Storage

IBI Isolate

4 mL

IB47630

Room Temperature

Dry at 2°C to 25°C for up to 9 months

80 mL

IB47631

160 mL

IB47632

W1 Buffer

2 mL

IB47630

Room Temperature

Dry at room temperature 
(15-25°C) for up to 9 months

50 mL

IB47631

130 mL

IB47632

Wash Buffer*
(Add Ethanol)

2 mL (8 mL)

IB47630

Room Temperature

Dry at room temperature 
(15-25°C) for up to 9 months

50 mL (200 mL)

IB47631

25 mL + 50 mL (50 mL + 200 mL)

IB47632

RNase-free Water

1 mL

IB47630

Room Temperature

Dry at room temperature 
(15-25°C) for up to 9 months

6 mL

IB47631

15 mL

IB47632

RB Columns

4 pcs

IB47630

Room Temperature

Dry at room temperature 
(15-25°C) for up to 9 months

100 pcs

IB47631

200 pcs

IB47632

2 mL Collection Tubes

8 pcs

IB47630

Room Temperature

Dry at room temperature 
(15-25°C) for up to 9 months

200 pcs

IB47631

400 pcs

IB47632

*Add absolute ethanol (see the bottle label for volume) to Wash Buffer prior to initial use.

IB47630

IBI Tri-Isolate Total RNA Kit, 4 Preps

IB47631

IBI Tri-Isolate Total RNA Kit, 100 Preps

IB47632

IBI Tri-Isolate Total RNA Kit, 200 Preps

SKU Number Type # of filters Price Quantity Add to Cart
MR9600-MID Microplate Absorbance Reader, 115V Includes 4 filters: 405, 450, 492, and 630nm
7103.20
Each
MR9600-E Microplate Absorbance Reader, 230V Includes 4 filters: 405, 450, 492, and 630nm
7103.20
Each
MR9600-PA Extra paper for printer, pk of 4 N/A
35.54
Each
MR9600-T Microplate Absorbance Reader w/ Incubation Includes 4 filters: 405, 450, 492, and 630nm
7561.64
Each
MR9600-TP Thermal Printer for MR9600 N/A
372.62
Each
MR9600-340 Filter 340 nm
312.61
Each
MR9600-380 Filter 380 nm
312.61
Each
MR9600-405 Filter 405 nm
235.00
Each
MR9600-415 Filter 415 nm
235.00
Each
MR9600-420 Filter 420 nm
235.00
Each
MR9600-450 Filter 450 nm
235.00
Each
MR9600-470 Filter 470 nm
235.00
Each
MR9600-492 Filter 492 nm
235.00
Each
MR9600-510 Filter 510 nm
235.00
Each
MR9600-520 Filter 520 nm
235.00
Each
MR9600-532 Filter 532 nm
235.00
Each
MR9600-540 Filter 540 nm
235.00
Each
MR9600-546 Filter 546 nm
235.00
Each
MR9600-560 Filter 560 nm
235.00
Each
MR9600-570 Filter 570 nm
235.00
Each
MR9600-578 Filter 578 nm
235.00
Each
MR9600-590 Filter 590 nm
235.00
Each
MR9600-595 Filter 595 nm
235.00
Each
MR9600-600 Filter 600 nm
235.00
Each
MR9600-620 Filter 620 nm
235.00
Each
MR9600-630 Filter 630 nm
235.00
Each
MR9600-650 Filter 650 nm
235.00
Each
MR9600-680 Filter 680 nm
235.00
Each
MR9600-690 Filter 690 nm
235.00
Each
MR9600-700 Filter 700 nm
235.00
Each
MR9600-750 Filter 750 nm
235.00
Each
Microplate Absorbance Rea
Accuris™ MR9600 SmartReader™ 96 Microplate Absorbance Reader
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