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Accuris™ MR9600 SmartReader™ 96 Microplate Absorbance Reader

IBI Tri-Isolate is a phenol and guanidine isothiocyanate plus spin column system for convenient purification of high-quality total RNA from a variety of samples. Initially, samples are homogenized in IBI Isolate without chloroform phase separation or isopropanol RNA precipitation. Following sample homogenization, simply bind, wash and elute the high-quality, total RNA in RNase-free Water and use in a variety of sensitive downstream applications.
Quality Control
IBI Tri-Isolate is tested on a lot-to-lot basis. An Escherichia coli (1 x 109) culture (OD600=1.3, 1 mL) is harvested by centrifugation at 16,000 x g for 2 minutes, followed by IBI Isolate homogenization. RNA is then purified using a spin column procedure. 10 µL from a 50 µL eluate of purified RNA is analyzed by electrophoresis on a 0.8% agarose gel.
Advantages
- Purify total RNA within 15 minutes without chloroform phase separation or isopropanol RNA precipitation
- Up to: 200 µL (blood, buffy coat, serum, plasma), 5 x 106 (cultured cells), 10-50 mg (tissue), 1 x 109 (bacteria cells)
- A cost effective phenol, guanidine isothiocyanate solution plus spin column system
- High quality RNA: A260/A280 >1.8, A260/A230 >1.8
- Applications: cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays,
- Northern Blotting
Caution
IBI Isolate contains phenol and guanidine isothiocyanate. During operation, always work in a fume hood, always wear a lab coat, disposable gloves, protective goggles and (anti-fog) procedure mask. Disposable/non-disposable glassware, plasticware and automatic pipettes should be sterile (RNase-free) and used only for RNA procedures.
Components and Storage
Item |
Volume |
Product |
Shipping |
Storage |
IBI Isolate |
4 mL |
IB47630 |
Room Temperature |
Dry at 2°C to 25°C for up to 9 months |
80 mL |
IB47631 |
|||
160 mL |
IB47632 |
|||
W1 Buffer |
2 mL |
IB47630 |
Room Temperature |
Dry at room temperature |
50 mL |
IB47631 |
|||
130 mL |
IB47632 |
|||
Wash Buffer* |
2 mL (8 mL) |
IB47630 |
Room Temperature |
Dry at room temperature |
50 mL (200 mL) |
IB47631 |
|||
25 mL + 50 mL (50 mL + 200 mL) |
IB47632 |
|||
RNase-free Water |
1 mL |
IB47630 |
Room Temperature |
Dry at room temperature |
6 mL |
IB47631 |
|||
15 mL |
IB47632 |
|||
RB Columns |
4 pcs |
IB47630 |
Room Temperature |
Dry at room temperature |
100 pcs |
IB47631 |
|||
200 pcs |
IB47632 |
|||
2 mL Collection Tubes |
8 pcs |
IB47630 |
Room Temperature |
Dry at room temperature |
200 pcs |
IB47631 |
|||
400 pcs |
IB47632 |
*Add absolute ethanol (see the bottle label for volume) to Wash Buffer prior to initial use.
IB47630 |
IBI Tri-Isolate Total RNA Kit, 4 Preps |
IB47631 |
IBI Tri-Isolate Total RNA Kit, 100 Preps |
IB47632 |
IBI Tri-Isolate Total RNA Kit, 200 Preps |

SKU Number | Type | # of filters | Price | Quantity | Add to Cart |
MR9600-MID | Microplate Absorbance Reader, 115V | Includes 4 filters: 405, 450, 492, and 630nm |
7103.20
Each
|
||
MR9600-E | Microplate Absorbance Reader, 230V | Includes 4 filters: 405, 450, 492, and 630nm |
7103.20
Each
|
||
MR9600-PA | Extra paper for printer, pk of 4 | N/A |
35.54
Each
|
||
MR9600-T | Microplate Absorbance Reader w/ Incubation | Includes 4 filters: 405, 450, 492, and 630nm |
7561.64
Each
|
||
MR9600-TP | Thermal Printer for MR9600 | N/A |
372.62
Each
|
||
MR9600-340 | Filter | 340 nm |
312.61
Each
|
||
MR9600-380 | Filter | 380 nm |
312.61
Each
|
||
MR9600-405 | Filter | 405 nm |
235.00
Each
|
||
MR9600-415 | Filter | 415 nm |
235.00
Each
|
||
MR9600-420 | Filter | 420 nm |
235.00
Each
|
||
MR9600-450 | Filter | 450 nm |
235.00
Each
|
||
MR9600-470 | Filter | 470 nm |
235.00
Each
|
||
MR9600-492 | Filter | 492 nm |
235.00
Each
|
||
MR9600-510 | Filter | 510 nm |
235.00
Each
|
||
MR9600-520 | Filter | 520 nm |
235.00
Each
|
||
MR9600-532 | Filter | 532 nm |
235.00
Each
|
||
MR9600-540 | Filter | 540 nm |
235.00
Each
|
||
MR9600-546 | Filter | 546 nm |
235.00
Each
|
||
MR9600-560 | Filter | 560 nm |
235.00
Each
|
||
MR9600-570 | Filter | 570 nm |
235.00
Each
|
||
MR9600-578 | Filter | 578 nm |
235.00
Each
|
||
MR9600-590 | Filter | 590 nm |
235.00
Each
|
||
MR9600-595 | Filter | 595 nm |
235.00
Each
|
||
MR9600-600 | Filter | 600 nm |
235.00
Each
|
||
MR9600-620 | Filter | 620 nm |
235.00
Each
|
||
MR9600-630 | Filter | 630 nm |
235.00
Each
|
||
MR9600-650 | Filter | 650 nm |
235.00
Each
|
||
MR9600-680 | Filter | 680 nm |
235.00
Each
|
||
MR9600-690 | Filter | 690 nm |
235.00
Each
|
||
MR9600-700 | Filter | 700 nm |
235.00
Each
|
||
MR9600-750 | Filter | 750 nm |
235.00
Each
|
