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Pall® Acrodisc® One Premium Syringe Filters
Item #:
ASACRODISCONE

TPP's Packed Cell Vol (PCV) tube enables a quick and reproducible method of cell counting based on cell Vol. A quick centrifuge of the cell suspension pipetted into a PCV tube reveals a packed cell pellet in the PCV capillary, which can be easily read with the aid of an Easy read ruler. This non-subjective method is superior to old-fashioned and labor intensive hemacytometer based counting.
Functional Features:
- Cell suspension sample can be directly quantitated without dilution
- Multiple samples can be assayed simultaneously
- Load and spin as many PCV tubes as your centrifuge rotor holds
- Quick and reproducible
- Cells can be spun down in 1 minute at 2500xg
Ergonomic Features:
- Reproducible measurements
- Graduated capillary model offers immediate quantitation
- For finer quantitation: The Easy Read Ruler is used with non-graduated PCV tubes
- Eliminates expensive automated counters / fragile slides
- Avoids subjective microscope counting
Quality Assurance:
- Gamma-sterilized, DNAse/RNAse-Free, and pyrogen-Free
- Individually wrapped in peel-off plastic wrapper
Protocol Example:
- Uniformly mix cell suspension collected from TPP flask/dish/bioreactor
- Pipet 1000µL of cell suspension into a PCV tube
- Spin multiple samples for 1 minute at 2500xg in a standard microcentrifuge
- Record pellet Vol and compare to pre-generated standard curve of cell numbers to packed cell Vol.
- Standard curve needs to be set up only once per cell line
- Pipet pre-determined cell suspension Vols (dilutions) into a hemacytometer
- Calculate and record the number of live cells for each concentration
- Pipet the identical Vols into the PCV tube, centrifuge and record the numeric in the capillary that corresponds to pellet Vol
- Plot the hemacytometer readings vs. the PCV readings for each Vol
- This Standard Curve doesn't need to be redone for the same cell line and moving forward the cell suspension harvested from TPP flasks/dishes/plates only need to be centrifuged in PCV tubes and assayed for cell pellet measurements
High density of cells
- With a very high density of the cells, the samples must not be diluted. You might simply reduce the sample Vol. Samples from the same series should be treated accordingly.
- For bacteria measurement the parameters have to be adjusted, for example higher g-force, smaller Vol, etc.
- Micro centrifuges with swing-out rotors are preferable.
Order# |
Description |
Quantity |
TP87005 |
PCV Cell Counting Tubes (with capillary graduations only) |
50/pk |
TP87007 |
PCV Cell Counting Tubes (no graduations) |
50/pk |
TP87008 |
TPP Cap for PCV Tubes |
50/pk |
TP87010 |
Easy Read Ruler |
1 |
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SKU Number | Filter Media | Filter Size | Outlet Type | Pore size | Qty | Price | Quantity | Add to Cart |
AP-4911 | wwPTFE | 25 mm | N/A | 0.2 µm | 25/pkg, 200/cs |
820.00
Each
|
||
AP-4910 | wwPTFE | 25 mm | N/A | 0.2 µm | 50/pkg, 200/cs |
842.07
Each
|
||
AP-4912 | wwPTFE | 25 mm | N/A | 0.2 µm | 1000/pkg |
2763.53
Each
|
||
AP-4914 | wwPTFE | 25 mm | N/A | GxF/0.2 µm | 25/pkg, 200/cs |
1038.80
Each
|
||
AP-4913 | wwPTFE | 25 mm | N/A | GxF/0.2 µm | 50/pkg, 200/cs |
933.86
Each
|
||
AP-4915 | wwPTFE | 25 mm | N/A | GxF/0.2 µm | 1000/pkg |
3290.48
Each
|
||
AP-4917 | wwPTFE | 25 mm | N/A | 0.45 µm | 25/pkg, 200/cs |
789.43
Each
|
||
AP-4916 | wwPTFE | 25 mm | N/A | 0.45 µm | 50/pkg, 200/cs |
789.43
Each
|
||
AP-4918 | wwPTFE | 25 mm | N/A | 0.45 µm | 1000/pkg |
2631.80
Each
|
||
AP-4920 | wwPTFE | 25 mm | N/A | GxF/0.45 µm | 25/pkg, 200/cs |
933.86
Each
|
||
AP-4919 | wwPTFE | 25 mm | N/A | GxF/0.45 µm | 50/pkg, 200/cs |
933.86
Each
|
||
AP-4921 | wwPTFE | 25 mm | N/A | GxF/0.45 µm | 1000/pkg |
3290.48
Each
|

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