For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

Cyto3D™ Live-Dead Assay Kit
 
Live dead cell viability analysis for 3D and 2D cell culture.

The Cyto3D™ Live-Dead Assay Kit is used to determine the live/dead nucleated cells by using a fast one-step staining procedure for analysis on a dual-fluorescence system. This kit is recommended for viability analysis of cells cultured in 3D, 2D coating and on monolayer.

• Ready-to-use
• Fast
• Sensitive
• Excellent for 3D cell cultures
• Cost-effective

Acridine orange (AO) and propidium iodide (PI), both nuclear staining (nucleic acid binding) dyes, are used in this kit. AO is permeable to both live and dead cells and stains all nucleated cells to generate green fluorescence. PI only penetrates the membranes of nucleated cells with compromised membranes and stains the dead cells to generate red fluorescence. Due to the quenching, when cells are stained with both AO and PI, all live nucleated cells fluoresce green and all dead nucleated cells fluoresce red (the PI reduces the fluorescence intensity of the AO by fluorescence resonance energy transfer (FRET)). Non-nucleated materials such as red blood cells, platelets and debris do not fluorescence and are ignored by fluorescence microscopes.

Dual-Fluorescence Viability, using AO and PI, is the recommended viability analysis method for cell lines, primary cells, and stem cells.

Specifications

TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.   

TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.

To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroGel® Angiogenesis Assay Kit
 
ready-to-use, hydrogel system for 2D gel coating and 3D culture of angiogenesis tube formation, invasion, and animal injection.

• Hydrogel - Hydrogel Only: VitroGel® AAK (2 mL)
• Type 1 - Full Kit (Hydrogel+Supplements): VitroGel AAK (2 mL), AAK Supplement 1 (500 µL), AAK Supplement 2 (500 µL)
• Type 2 - Full Kit (Hydrogel+Supplements): VitroGel AAK (2 mL), AAK Supplement 1 (2 x 500 µL)
• Type 3 - Full Kit (Hydrogel+Supplements): VitroGel AAK (2 mL), AAK Supplement 2 (2 x 500 µL)

• Xeno-free hydrogel system supporting angiogenesis process
• Multiple applications in one kit:  Tube formation, invasion, and animal injection
• Control the hydrogel properties:  Add your own growth factors and compare with positive control
• Easy, efficient, and enzyme-free cell harvesting in 20 minutes

VitroGel® Angiogenesis Assay Kit is a revolutionary tool for researchers to study the effect of both hydrogel properties and culture medium on angiogenesis process. The kit can be used to study the angiogenesis tube formation and invasion on both 2D hydrogel coating method and 3D cell culture method. The VitroGel® system is also good for animal injection for in vivo study.

Angiogenesis is a highly regulated process that involves the growth of new blood vessels from the existing vasculature. This process plays an important role in both normal developmental processes and numerous pathologies, including wound healing, tumor growth, and metastasis to inflammation and ocular disease.

Traditional angiogenesis assay highly relies on natural extracellular matrix (natural ECM), which has non-adjustable hydrogel compositions and properties.  Therefore, our understanding of the angiogenesis process is limited by studying the molecular cues such as growth factors and inhibitors in culture medium only. There is a lack of knowledge on how the properties of hydrogel affect the angiogenesis process.

There are two versions of VitroGel® Angiogenesis Assay Kits:

• VitroGel® Angiogenesis Assay Kit (Ready-To-Use):  Ready-to-use with a fixed hydrogel mechanical strength to support the angiogenesis assay with adjustable supplements
• VitroGel® Angiogenesis Assay HC Kit (Cat No. TWG011-K):  Assay kit with a tunable high concentration hydrogel to allow full control of the hydrogel’s mechanical strength with adjustable supplements.

Depending on the kit type, the ready-to-use VitroGel® Angiogenesis Assay Kits can contain the following components:

• VitroGel® AAK, a xeno-free ready-to-use hydrogel.
• AAK Supplement 1, a hydrogel supplement without vascular endothelial growth factors (VEGFs) for cell attachment and growth.
• AAK Supplement 2, a hydrogel supplement with VEGFs to promote tube formation and as a positive control.

The VitroGel® AAK hydrogel is room temperature stable and can be directly mixed with each supplement at 2:1 (v/v) ratio for hydrogel formation.  Researchers can adjust the hydrogel’s molecular cues by adding the growth factors/inhibitors directly to the supplements before mixing with the VitroGel® AAK hydrogel.  Cells cultured in this system can be harvested further easily with the VitroGel® Cell Recovery Solution.

Contents and Specifications

TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.   

TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.

To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.