Home  >  Chemicals  >  Fluorescent, Dyes and Assays
|◀ ◀ 1 - 12 of 48 ▶ ▶|
View:

PR1MA™ Rapid Coomassie Stain

Chemical Formula: C5H10O5

Solubility: Water

Storage Temp: Room Temperature

This unique Coomassie Blue based formulation features a special dye that is absorbed by protein bands instead of the gel matrix. This eliminates the need for destaining and offers enhanced sensitivity and sharper resolution of bands. Protein bands can be detected within 10 minutes. Maximum intensification may require incubation up to 40 minutes. Offers greater convenience and sensitivity than conventional stains. Compatible with silver staining. Rapid Coomassie stain uses a simple two-step procedure. Stain is designed to detect as little as 7.9ng of protein within one hour of electrophoresis.

Supplied as 20X concentrated solution.

Shelf life is approximately 18 months at room temperature.

Shipping Weight: 0.3 lbs

Shipping Dimensions: 2.00 x 4.00 x 2.00
  • Appearance: Dark Blue Color
  • Concentration: 20X
  • Solubility: Suitable for use in staining protein in polyacrylamide gels
Research or further manufacturing use only, not for food or drug use.
 

Proudly made in the USA! Click here for more made in America products!
  

Item#:
ASRAPIDCOMMSTN
 
PR1MA Safety Data Sheet KCRCS-50_RCS-50


Radiance ECL Chemiluminescent Substrate

 

Chemiluminescent HRP substrate for chemiluminescent Western blots

Radiance ECL is an HRP substrate with long-lasting signal and sensitivity down to low picograms. Use up to 10 times less antibody than other substrates used in life science.

 

Size: 500 mL

 

Features

  • Performance — compare performance to SuperSignal West Pico and Amersham ECL
  • Sensitivity — high sensitivity in low picograms
  • Duration — Signal duration 6-8 hours
  • Chemiluminescent Western Blotting
  • The most common method for Western blot detection is chemiluminescence. Detection by chemiluminescence is relatively sensitive and works well for detecting individual or low abundance proteins.   

Item#:
ASRADIANCEECL
 
Radiance ECL Safety Data Sheet

Radiance Plus Chemiluminescent Substrate

 

The most sensitive HRP substrate available for chemiluminescent Western blotting.

With attomole sensitivity and a long-lasting signal, Radiance Plus allows you to detect bands not visualized with other substrates. High signal-to-noise and a large dynamic range make it ideal for quantifying low-intensity bands.

 

Size: 150 mL

 

Features

  • Low-abundance protein detection
  • Compare performance to competitor products, like SuperSignal West Femto and Amersham ECL Select
  • Sensitivity in low femtograms
  • Signal duration 6-8 hours   

Item#:
ASRADIANCEPLUS
 
Radiance Plus Safety Data Sheet

Azure Flash Western Chemiluminescence Kit

 

Kit includes all reagents necessary for performing ten chemiluminescent Western blots using the Flash Western protocol

 

Each kit includes:

  • 10x PVDF Membranes
  • 100mL Chemi Blot Blocking Buffer
  • 500μL Flash HRP Conjugate
  • 250mL Flash Wash Buffer
  • 100mL Radiance ECL Substrate

Features

 

Time savings using Azure Flash Western Chemiluminescent Kit. Flash Western graph includes use of Azure Transfer Buffer in light blue.

 

The Azure Flash Western Kit uses a proprietary set of reagents to achieve complete membrane blocking, rapid hybridization of antibodies, and enhancement of chemi signal in less than an hour of blotting time. The included transfer buffer cuts wet transfer time down to 15 to 30 minutes for a total assay time of two hours from gel to imaging.

  • Fast protocol with few hands-on steps
  • Antibody stabilization for enhanced chemi signal
  • Low background and increased signal-to-noise ratios
  • Increased detection sensitivity and linear dynamic range

 

 

Item#:
ASAZFLASHWESTKIT
 
Radiance Q - SDS
Radiance Peroxide - SDS
Flash HRP Conjugate - SDS
Flash Wash Buffer - SDS
Flash Buffer A - SDS


Radiance Q Chemiluminescent Substrate

 

Radiance Q chemiluminescent substrate is specially formulated for use with Azure Imaging Systems, or other CCD imagers. This novel horseradish peroxidase (HRP) substrate provides a strong, long-lasting signal, the broadest useful linear range, and high sensitivity for the most quantitative chemiluminescent Western assays.

 

Size: 150 mL

 

Features
 

Increase the sensitivity and quantitative accuracy of your chemiluminescent Western blots. Specially developed for CCD imaging, Radiance Q produces a strong, long-lasting signal with extremely low background, perfect for detecting low abundance proteins. Because the substrate is not depleted at high protein loads, Radiance provides the largest dynamic range of any chemiluminescent substrate, improving quantitative accuracy.

  • Extended dynamic range for detecting low and high abundance proteins within the same blot
  • Compare performance to SuperSignal West Dura and Amersham ECL Prime
  • Sensitivity in mid femtograms
  • Signal duration 10-24 hours   

Item#:
ASRADIANCEQ
 
Radiance Q Safety Brochure
Radiance Q Safety Data Sheet
ION Biosciences IPG-4 AM

Yellow-green fluorescent, potassium (K+) indicator, membrane permeable.
 
Higher K+ affinity than IPG-1 and IPG-2
 
ION Potassium Green – 4 (IPG-4) is a yellow-green fluorescent, intracellular potassium (K+) indicator with Ex/Em: 525 nm/545 nm and a high-sensitivity to detect small changes in K+ concentration. IPG-4 has the highest affinity (Kd = 7 mM) among IPG analogues – IPG-2 (Kd = 18 mM) and IPG-1 (Kd = 50 mM).
Item#:
ASIPG4AM
 
IPG-4 AM Product Specifications
IPG-4 AM Safety Data Sheet
ION Biosciences BAPTA AM
 
Non-fluorescent calcium (Ca²+) chelator, membrane permeable.
 
BAPTA AM is a highly selective, intracellular calcium (Ca²+) chelator that is less sensitive to pH fluctuations than EGTA and EDTA. Can be used to modulate the level of intracellular Ca²+.
Item#:
ASBAPTAAM
 
BAPTA AM Safety Data Sheet
BAPTA AM Product Specifications

Reporter gene based Luminescent assay systems from Perkin Elmer provide a homogeneous assay with 96, 384, and 1536 well formats, no DTT (no smell formulation), Ease of storage (2 to 8°C) and shipping (at RT). Different assay formats allow for varying levels of intensity and half-life depending on your application.

  • Choose britelite plus:
    • Half-life = 30 min
    • Brightest signal = when looking for maximum sensitivity
    • Shortest half-life = for continuous processing of samples
  • Choose neolite:
    • Half-life = 150 min
    • High signal intensity, longer lasting signal = good balance
    • For both continuous and batch processing of samples
    • Best robustness (less dependent on mixing quality)
    • Less sensitive to phenol red
    • Can be stored 1 month at 22°C
  • Choose steadylite plus:
    • Half-life = 240 min
    • Longest signal half-life = for batch processing of samples
    • Super-sensitivity not needed
Item#:
ASLUMASSAY
SBFI K+ SALT
 
Ratiometric sodium (Na+) indicator, membrane impermeable.
 
SBFI is a UV-excitable, ratiometric green indicator for intracellular sodium (Na+) measurements. Ex/Em: 340/505 nm can be used to measure Na+-bound SBFI, and Ex/Em: 380/505 nm can be used to detect Na+-free SBFI. It is ~18X more selective for Na+ over K+. Ratiometry is optimal for imaging applications where quantification of intracellular Na+ concentrations is desired, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.
SBFI K+ salt is a membrane impermeable form of SBFI that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.
Item#:
ASSBFIKST
 
SBFI K+ Salt Safety Data Sheet
SBFI K+ Salt Product Specifications
ION Biosciences Fluo-4 K+ Salt
 
Green fluorescent, calcium (Ca²+) indicator, membrane impermeable.
 
Fluo-4 is the most popular green fluorescent, calcium (Ca²+) indicator. Proven and trusted for decades to measure intracellular Ca²+ flux in high throughput screening and fluorescence microscopy applications.

Fluo-4 K+ Salt is a membrane impermeable form of Fluo-4 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.
Item#:
ASFLUO4AMST
 
Safety Data Sheet Fluo-4 K+ Salt
Product Specifications Fluo-4 K+ Salt

ION Vital - Viability Kit

Two-color, fluorescence-based assay to discriminate between live and dead cells.
 
ION Vital – Viability kit can be used to quantify live and dead cells within eukaryotic cell suspensions or adherent cultures, 3D cultures, organoids, and some non-fixed tissues. Cannot be used for yeast or bacteria. When following our protocol, ION Vital—Viability kit provides enough reagents to make 150 mL of working solution, enough for fifteen 96-well plates or 1,500 flow cytometry samples. The actual number of assays will vary according to optimal dye concentrations for your application.
Item#:
ASAIONVIT
 
ION VITAL VIABILITY PROTOCOL
ION VITAL VIABILITY Safety Data Sheet
ION Biosciences PBFI K+ Salt
 
PBFI is a UV-excitable, ratiometric green indicator for intracellular potassium (K+) measurements. Ex/Em: 340/505 nm can be used to measure K+-bound PBFI, and Ex/Em: 380/505 nm can be used to detect K+-free PBFI. It is 1.5X more selective for K+ over Naand works well in cytosolic conditions where [K+]/[Na] is ~10. Ratiometry is optimal for imaging applications where quantification of intracellular K+ concentrations is desired and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.

PBFI K+ salt is a membrane impermeable form of PBFI that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection, or other methods.
Item#:
ASPBFIKST
 
PBFI K+ Salt Safety Data Sheet
PBFI K+ Salt Product Specifications
|◀ ◀ 1 - 12 of 48 ▶ ▶|
View: