Hoefer® Air-Cooled Vertical Electrophoresis System

Compact, rugged, and economical units ideal for educational laboratories with multiple users.

• Maximum glass plate size 18 x 16 cm
• Rugged injection molded construction is durable and assembles easily
• Run two gels with the addition of a divider plate
• Built-in casting stand seals leak free
• Choose from a wide variety of accessories
• Plates, clamps, spacers, and combs are interchangeable with the SE600 Series 

Hoefer® Air-Cooled Complete Unit (SE400-15-15)

• Lower Buffer Chamber/Casting Stand on Leveling Base with High Voltage Leads, 1/ea
• Upper Buffer Chamber, 1/ea
• Safety Lid with Electrodes, 1/ea
• Glass Plates, 18 x 16 cm, 2/pk
• Clamp Assemblies, 16 cm, 2/pk
• Cams, 2/pk
• Slotted Gasket for Upper Buffer Chamber, 1/ea
• Bubble Level, 1/ea
• Laminated Gasket for Casting Stand, 1/ea
• Spacer-Mate-Alignment Template, 1/ea
• Wonder Wedge Plate Separation Tool, 1/ea
• Gel Seal, 1/ea
• Comb, 15-well, 1.5 mm thick, 1/ea
• Spacers, 1.5 mm thick, 2 cm wide, 2/pk 

Hoefer® Air-Cooled Basic Unit (SE400)

The same as above without the combs and spacers. Order one SE511 comb and one set of 16 cm long spacers separately.

Specifications
 

Plate Size (W x H)	7.09 x 6.30 In. | 18 x 16 cm
Spacers	0.75 mm, 1.0 mm, 1.5 mm
Max. Power	500 V, 60 mA, 20 W
Max. Temp.	113ºF | 45°C
Indoor Use	39.2 to 104ºF | 4 to 40°C
Humidity	Up to 80%
Dims (D x W x H)	5.91 x 9.45 x 11.02 In. | 15 x 24 x 28 cm
Weight	6.83 | 3.10 kg

Hoefer® Max Horizontal Agarose Electrophoresis System

Offers a variety of gel lengths and comb sizes to enable the design of the gel required for optimum resolution of DNA fragments. Facilitates high-resolution separation of nucleic acid fragments 100 bp to 20 kbp. Three different length trays are available for use with this unit. 

• Self-locating/centering guides support consistent running tray placement
• Gel anchor feet prevent the gel from floating or sliding during the run
• A variety of comb sizes enables customization of sample throughput to fit your laboratory's requirements
• Adjustable comb height—complete control over well depth
• Three casting kits available—enabling users to run 15 x 20, 15 x 15, or 15 x 10 cm gels in the same unit
• Gel Casting Kit Includes 15 x 10, 15 x 15, or 15 x 20 cm Running Tray, 15 x 10, 15 x 15, or 15 x 20 cm Casting Tray, and 4 Foam Gaskets

Complete Unit (HE99X-15-15)

• Buffer Chamber Assembly, 1/ea
• Safety Lid with High Voltage Leads, 1/ea
• Running Tray, 15 x 20 cm, 1/ea
• Casting Tray, 15 x 20 cm, 1/ea
• Foam Gaskets, 4/pk
• Bubble Level, 1/ea
• 1.5 mm thick, 15-well comb, 1/ea
• Comb Back, 1/ea

Basic Unit (HE99X)

The same as above, without the Running Tray, Casting Tray, Comb, and Comb Back. Order a casting kit, combs, and comb backs separately.

Specifications
 

Gel Dims (W x L)	5.91 x 7.87 | 5.91 x 5.91 | 5.91 x 3.94 In. 15 x 20 | 15 x 15 | 15 x 10 cm
Max. Buffer Vol.	1.2 L
Max. Power	200 V, 100 mA, 20 W
Max. Capacity	60 Samples
Max. Temp.	113ºF | 45°C
Indoor Use	39.2 to 104ºF | 4 to 40°C
Humidity	Up to 80%
Dims (D x W x H)	14.17 x 7.17 x 5.51 In. | 36 x 18.2 x 14 cm

Hoefer® Mighty Small Transfer Tank

The TE22 Mighty Small Transfer Tank quickly and efficiently transfers proteins and nucleic acids from small gels to nylon, nitrocellulose or PVDF membranes. Maximum Gel Size 9 x 10 cm.

• Transfer as many as four small gels, up to 9 x 10 cm in less than an hour
• Uniform and strong electric field—supports efficient and even transfers
• Color-coded, easy to assemble and load cassettes—ensures proper orientation during transfer
• Superior tank design allows the cassettes to apply equal pressure across the stack - prevents gel distortion
• Built-in ceramic heat exchanger—provides excellent temperature control when used with an external cooling water bath
• Built-in alumina-covered cooling channel provides excellent temperature control with no more than a 5°C temperature increase during a typical run

Hoefer® Mighty Small Transfer Tank Includes

• Lower Chamber with Heat Exchanger, 1/ea
• Safety Lid with High Voltage Leads, 1/ea
• Electrode Panels, 2/pk
• Cassettes, 4/pk
• Foam Sponges, 6 mm thick, 4/pk
• Foam Sponges, 3 mm thick, 8/pk
• Cassette Hook, 1/ea
• Blotter Paper (9 x 10.5 cm), 25 sheets/pk

Hoefer® Mini Electrophoresis and Western Blot Package

Specifications
 

Capacity	Up to four 9 x 10 cm gels
Max. Power	100 V, 500 mA, 50 W
Max. Temp.	113ºF | 45°C
Indoor Use	39.2 to 104ºF | 4 to 40°C
Humidity	Up to 80%
Dims (D x W x H)	6.50 x 5.51 x 9.45 In. | 16.5 x 14 x 24 cm
Weight	4.441 lb | 2.00 kg

Hoefer® Standard Transfer Tank

The TE42 Standard Transfer Tank quickly and evenly transfers proteins and nucleic acids from polyacrylamide or agarose gels onto nylon, nitrocellulose, or PVDF membranes. The four slot design of the TE42 enables either cooled or non-cooled protein or nucleic acid transfers. The optional heat exchange module is easily incorporated into the TE42 enabling transfer of up to 2 gels simultaneously. When used without the optional heat exchange module, up to four gels can be transferred at one time.

• Uniform and strong electric field—supports efficient and even transfers
• Color-coded, easy to assemble cassettes—ensures proper orientation during transfer
• Superior tank design allows the cassettes to apply equal pressure across the stack - prevents gel distortion
• Optional TE47 heat exchanger—provides excellent buffer temperature control when used with an external cooling water bath
• Heat exchanger from an SE600 series electrophoresis unit can be substituted for the TE47 heat exchanger. 

Hoefer® Standard Transfer Tank Includes

• Lower Chamber, 1/ea
• Safety Lid with High Voltage Leads, 1/ea
• Electrode Panels, 2/pk
• Cassettes, 2/pk
• Foam Sponges, 6 mm thick, 2/pk
• Foam Sponges, 3 mm thick, 4/pk
• Cassette Hook, 1/ea
• Blotter Paper (14.5 x 21.5 cm), 25 sheets/pk

Specifications
 

Capacity	Up to four 15 x 21 cm gels (w/out cooling) Up to two 15 x 21 cm gels (w/ cooling)
Max. Power	100 V, 2 A, 200 W
Max. Temp.	113ºF | 45°C
Indoor Use	39.2 to 104ºF | 4 to 40°C
Humidity	Up to 80%
Dims (D x W x H)	12.01 x 3.15 x 5.12 In. | 30.5 x 8 x 13 cm

Hoefer® Standard Transfer Tank with Cooling Chamber

TE62 Standard Transfer Tank with Cooling Chamber quickly and evenly transfers proteins and nucleic acids from polyacrylamide or agarose gels onto nylon, nitrocellulose, or PVDF membranes while providing excellent temperature control.

The TE62 is designed to accept up to four cassettes simultaneously with cooling. Each cassette holds a 15 x 21 cm gel, or as many as four 7 x 10 cm mini gels.

• Uniform and strong electric field—supports efficient and even transfers
• Color-coded, easy to assemble cassettes - ensures proper orientation during transfer
• Superior tank design allows the cassettes to apply equal pressure across the stack - prevents gel distortion
• Built-in ceramic heat exchanger - provides excellent temperature control when used with an external cooling water bath  

Hoefer® Standard Transfer Tank with Cooling Chamber Includes

• Lower Chamber with Heat Exchanger, 1/ea
• Safety Lid with High Voltage Leads, 1/ea
• Electrode Panels, 2/pk
• Cassettes, 4/pk
• Foam Sponges, 6 mm thick, 4/pk
• Foam Sponges, 3 mm thick, 8/pk
• Cassette Hook, 1/ea
• Blotter Paper (14.5 x 21.5 cm), 25 sheets/pk 

Specifications
 

Capacity	Up to four 15 x 21 cm gels Up to sixteen 7 x 10 cm gels
Max. Power	100 V, 2 A, 200 W
Max. Temp.	113ºF | 45°C
Indoor Use	39.2 to 104ºF | 4 to 40°C
Humidity	Up to 80%
Dims (D x W x H)	12.60 x 11.02 x 6.50 In. | 32 x 28 x 16.5 cm

Azure Aqua Transfer Cell

Perform a quick 1 hour transfer or transfer overnight at a lower voltage

Aqua Transfer Cell is used for transferring two mini gels to membranes for Western blotting experiments (wet transfer). The transfer cassettes and electrode core are colored for directionality which makes transferring easy and straightforward.

Includes:

• 2 transfer cassettes
• 6 sponges
• 2 Azure Aqua cooling units
• Tank and upper lid with power cables
• Transfer Electrode Cores

Features

The transfer cassettes and electrode core are colored for directionality, making transferring easy and straightforward.

Chemical reagent compatibility

Keep all components of the Azure Aqua Transfer Cell away from chloroform, arenes (such as methylbenzene benzene), and acetone. Any damage caused by these reagents is not covered by the warranty policy.

 
Cleaning Instructions

Before using, wash all components with a neutral detergent and rinse well with ultra pure water.

Wash the electrode assembly, transfer cassettes, and buffer tank with a neutral detergent and warm water. Be careful to avoid damaging the platinum electrodes. Use warm water to rinse the sponges, and then rinse all components with distilled or ultra-pure water.

Specifications
 

Azure Aqua Quad Mini-Cell

Vertical Gel Running System

Designed for running 1–4 precast or hand-cast gels (cassette size 10cm x 8cm) for electrophoresis. The locking side fasteners provide a tight seal that ensures rapid and easy electrophoresis. The unit comes equipped with 4 gel casting stands and casting frames, perfect for hand casting your own gels.

Simply align the spacer and short glass plates, seal between the hinges, and allow your gel to polymerize. Insert the polymerized gel into the electrophoresis module for an efficient gel-running experiment.

Includes:

• 4 gel casting stands and casting frames buffer dam
• 5 spacer plates (1mm)
• 10 short glass plates
• 5 15-well combs
• 5 10-well combs
• 5 sealing gaskets
• 5 gel shovels
• Tank and lid with power cables
• Primary and Secondary Electrode Cores

Features

• The Azure Aqua Quad Mini-Cell is designed for running 1–4 precast or hand-cast gels (cassette size 10cm x 8cm) for electrophoresis. The locking side fasteners provide a tight seal that ensures rapid and easy electrophoresis.
• The unit comes equipped with 4 gel casting stands and casting frames, perfect for hand casting your own gels: Simply align the spacer and short glass plates, seal between the hinges, and allow your gel to polymerize.
• Insert the polymerized gel into the electrophoresis module for an efficient gel-running experiment.

Specifications
 

Parameters	Azure Aqua Quad Mini-Cell Specs
Unit dimensions (W x L x H)	13.5 cm x 17.5 cm x 19 cm
Weight	2.2 lbs
Recommended power supply	Azure Aqua Power Supply 600V
Number of gels	1-4 precast (most major brands) or handcast gels
Tank buffer volume	2 gels: 540 mL | 4 gels: 1100 mL
Maximum gel size	6.7 cm x 8.6 cm
Typical run times	30-45 minutes at 200V
Gel cassette size (WxL)	10 cm x 8 cm

 

Azure Aqua Power Supply

Designed for powering electrophoresis and Western blotting transfer modules

This power supply can create and save protocols for all your gel and blotting experiments. Designed with you in mind, features a pause/resume function for flexibility.

Power supply includes output range of 5–600V, 1–1200mA, 1–500W, LCD readable voltage, current, power, and four power connections to run multiple experiments at once. Able to control constant power, current, or voltage.

Features

The Azure Aqua Power Supply is a universal power supply that is designed for powering electrophoresis and transfer modules. The power supply can create and save protocols for all your gel and blotting experiments and includes a pause/resume function for flexibility.

Azure Power Supply includes an output range of 5-600V, 1-1200mA, 1-500W, LCD readable voltage, current, power, and 4 connections for electrophoresis and transfer.

Specifications
 

Accuris SmartBlue™ Transilluminators

Accuris SmartBlue™ Transilluminators have been developed for viewing DNA gels stained with new, safe fluorescent dyes.  These new dyes, such as SmartGlow™, SYBR Green, Gel Green, have an excitation wavelength of 465-470 nm (visible blue light), and are safer to use than Ethidium Bromide.

• Blue light, safe for nucleic acids
• Uniform illumination (UI Technology)
• Optimal wavelength (465nm) for common safe stains
• Adjustable position, amber filter covers
• Optional amber viewing glasses: E4000-VG1

Safer for samples, safer for you: Accuris SmartBlue™ Transilluminators are the perfect partner for the most common safe DNA stains. These instruments emit blue light at a wavelength of 465 nm, perfect for excitation of most green fluorophors, including our Accuris’ SmartGlow™, SYBR Green, SYBR Safe, SYBR Gold, GelStar, GelGreen, to name a few.  SmartBlue™ transilluminators can also work for excitation of EtBr (though fluorescence is not as bright as with UV), see our Technical Bulletin with sample images.   Exposing DNA to blue wavelength does not cause molecular damage, and results in a much higher downstream cloning efficiency as compared to even short UV light exposures.  In addition, the visible blue light is not damaging to the user’s skin and is less harmful to the eyes.*

Adjustable filter cover: Both the E4101 and SB4500 transilluminators come with emission filter covers. These amber covers, which rest above the viewing surface and gel, filter out the blue light, allowing the fluorescing DNA bands to be seen clearly.  The covers can be set to a flat position for general gel viewing or can be set at an angle to provide easy access to the gel for band excision.  The SB4500 has a two-position filter cover, and the E4101 features a hinged filter cover.

Optional amber viewing glasses: Our E4000-VG1 SmartBlue™ Plus Viewing Glasses offer efficient blocking of the blue light wavelength from the transilluminators, allowing clear visualization of the fluorescing DNA bands without the filter cover in place.  These convenient glasses are recommended for users that are frequently excising DNA bands from gels.  SmartBlue™ Plus glasses also block 100% of UV wavelength so they can also be used for viewing gels on a UV transilluminator.

UI technology, bright and clear images: The bright LED light plates and filter layer provides an extremely evenly lit viewing surface. Even in ambient light, bands of DNA can clearly be seen in stained gels. Images obtained using the SmartBlue™ Transilluminator are brighter and more balanced than those obtained using epi-illuminators which light from the sides.

Reliable, durable, and long-lasting: An array of super bright LEDs with a long, 30,000 hour service life provide the light source for the SmartBlue™ Transilluminators. Unlike those in a UV transilluminator, the filters in the SmartBlue™ systems will not solarize and degrade in performance over time. Gel bands can be excised directly on the scratch resistant, glass viewing surfaces. To save energy, the power switch includes an automatic 5 minute shutoff. SmartBlue™ Transilluminators are covered by a 2 year warranty.

* SmartBlue™ products use high intensity LED lights.  Although the visible blue wavelength is significantly safer than UV light, it is recommended to not stare directly at the viewing surface without the amber cover in place. 

Specifications 

Light Source	High Intensity Blue LEDs
Emission Filter Cover	Amber
Wavelength	Peak at 465 nm
E4000 Viewing Surface	17 x 12 cm
E4000 Ext. Dimensions	30.5 x 21.5 x 5 cm
E4101 Viewing Surface	10.5 x 10.5 cm
E4101 Ext. Dimensions	17 x 12 x 5 cm
Electrical	100 to  240V, 50-60Hz
E4000 Weight	1.2kg / 2.6lbs
E4101 Weight	0.34kg / 0.75lbs
Warranty	2 years

 
 Item # SB4500 is proudly made in the USA! Click here for more made in America products!
 

Hoefer® Semi-Dry Transfer Units

Hoefer® Semi-Dry Transfer Units are efficiently designed to use minimal buffer to transfer proteins from polyacrylamide gels onto membranes (either nitrocellulose or PVDF) in less than an hour. They support as many as two layers of gels being transferred simultaneously. The TE70XP has a 14 x 16 cm maximum transfer area, which can handle up to four mini gels using the stacked format. The TE77X and TE77XP have a 21 x 26 cm transfer area for a capacity of up to 12 mini gels at one time. 

• Included safety circuit breaker—limits voltage and current from the user's power supply, preventing electrical damage to the transfer unit
• Requires minimal current—does not generate the excessive heat that can dry out the transfer stack and halt transfer or damage transfer units 
• Durable iridium oxide and stainless steel electrodes—allow for contamination free, consistent transfer
• Vented electrodes—prevent buildup of bubbles which may impair transfer
• Minimal buffer requirements—reagent cost and preparation time are reduced
• The TE70XP and TE77XP Semi-Dry Transfer Units have an intelligent built-in power supply that prevents the stack from overheating by monitoring the transfer status 

All units include

• Molded Base with Platinum-Coated Titanium Anode and High Voltage Leads
• Hinged Lid with Stainless Steel Cathode  

TE77X includes

• Blotting Paper (21 x 26 cm), 25/pk
• Porous Cellophane (20 x 35.5 cm), 50/pk
• Mylar® Masks (23 x 27.5 cm), 2/pk
• Hinged Lid with Stainless Steel Cathode, 1/ea

TE70XP includes

• Blotting Paper (14 x 16 cm), 25/pk
• Porous Cellophane (20 x 35.5 cm), 50/pk
• Mylar® Masks (16.5 x 18.5 cm), 2/pk 

TE77XP includes

• Blotting Paper (21 x 26 cm), 25/pk
• Porous Cellophane (20 x 35.5 cm), 50/pk
• Mylar® Masks (23 x 27.5 cm), 2/pk

Specifications
 

Item #	TE77X	TE70XP	TE77XP
Transfer Area	Up to 21 x 26 cm Up to 8.27 x 10.24 In.	Up to 14 x 16 cm Up to 5.51 x 3.60 In.	Up to 21 x 26 cm Up to 8.27 x 10.24 In.
Max. Power	30 V, 500 mA, 15 W
Max. Temp.	113ºF | 45°C
Indoor Use	39.2 to 104ºF | 4 to 40°C
Humidity	Up to 80%
Dims (D x W x H)	3.54 x 14.96 x 18.11 In. | 9 x 38 x 46 cm
Weight	16.31 lb | 7.40 kg

Hoefer® Gradient Makers

Ideal for generating gradients of polyacrylamide, sucrose, and cesium chloride.

• Made of heavy acrylic plastic with high-quality, leak-free Teflon® valves
• Flat base provides stability on the magnetic stir. Comes with a support rod for attaching to a ring stand
• Takes Luer or 4 mm ID tubing. With included adapters, it can also take 2 mm ID or 22-gauge tubing

SG15, SG30, SG50 and SG100 include

• Gradient Maker with Teflon® Valves, 1/ea
• Adapter Barbed Fitting, 1/ea
• Luer Outlet Fitting, 1/ea
• 22-gauge Needle, 1/ea
• Support Rod, 1/ea 

The 500 mL SG500 Gradient Maker is made of two cylindrical acrylic chambers joined and mounted on a flat acrylic base.

• An easy-to-use push-pull valve opens and closes the passage between the reservoir and mixing chambers
• Suitable for forming polyacrylamide gradients or buffer gradients used with medium to small chromatography columns
• Ideal for use with the SE615 Multiple Gel Caster  

SG500 includes

• Gradient Maker with Push-Pull Valve, 1/ea
• Hose Clamps, 4/pk

Proudly made in the USA! Click here for more made in America products!
 

Hoefer® Gel Casters

Enable several gradient or homogeneous gels to be cast simultaneously. Choice of multiple-gel casters or dual-gel casters.

Gel Casters for Small Format Vertical Electrophoresis Systems (SE250, SE260, SE300)

Multiple-Gel Casters

• Pour uniform gels from the top; pump gradient gels into an inlet port at the bottom of the unit
• Sandwiches fill at the same time and at the same rate from the same solution
• The result: identical gels
• Glass plate sandwiches seal leak-free without the use of messy grease or tape
• Once cast, gels can be wrapped in plastic and refrigerated for several weeks

Dual-Gel Caster (SE245)

• Cast one or two gels
• Accommodates both 10 x 8 cm and 10 x 10.5 cm plates

*For use with the SE250 and SE260 Series only.
 

Gel Casters for SE600 and SE400 Series Vertical Units   

Multiple-Gel Casters

• SE675 casts up to four 16 cm long gels
• SE615 casts up to ten 16 cm long gels
• Easy to use and leak-free
• Cast homogeneous or gradient gels. Pour homogeneous gels directly into the top of the casting chamber: pump gradient gels through an inlet port at the bottom

Dual-Gel Caster (SE6015)

SE6015 Dual Gel Caster is included when you order an SE600 series unit. It can also be ordered separately.

• Casts two 8, 16, or 24 cm long gels at a time
• Double the capacity with optional divider plates
• Easy to use and leak-free
• Glass Sandwichers seal into the casting 

*For use with the SE600 Series only.

Proudly made in the USA! Click here for more made in America products!