PR1MA™ MS2 Phage

PR1MA™ MS2 is an E. coli bacteriophage with a single-stranded RNA genome of 3569 nucleotides protected from nuclease degradation by a capsid of 180 coat protein monomers. This virus is a Biosafety Level 1 organism, not pathogenic to humans. These properties make MS2 phage useful as a process control in nucleic acid-based amplification techniques like RT-PCR, RT-LAMP, particularly those that involve viral RNA extraction.

• Biosafety level 1
• Lysis: 65°C for 20 minutes or by standard RNA extraction
• Storage temperature: 4°C 

Buffer composition

• 10 mM Tris-HCl
• 0.1  mM EDTA
• pH = 8.0 

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.
 

Proudly made in the USA! Click here for more made in America products!
  

PR1MA™ RNase Inhibitor

PR1MA™ RNase Inhibitor (RI) is a 50 kDa protein that specifically inhibits RNases A, B, and C by binding noncovalently in a 1:1 ratio at high affinity. It can be used in isothermal amplification and molecular diagnostic assays, cDNA synthesis, and other applications where RNA stability is important. It is ineffective against RNase1, T1, S1 Nuclease, or RNase H. It has no inhibition of polymerase activity when used with Taq DNA polymerase, AMV, M-MuLV, HIV reverse transcriptases, or phage RNA polymerases.

Storage Buffer

• 50% glycerol
• 10 mM Tris-HCl
• 50 mM KCl
• 8 mM DTT
• pH = 7.5 

*To prevent the release of ribonuclease from RNase Inhibitor, temperatures greater than 50°C and high concentrations of denaturing reagents such as urea should be avoided.

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.

Proudly made in the USA! Click here for more made in America products!
  

PR1MA™ β - Agarase

PR1MA™ β - Agarase has a high tolerance to inhibitors in electrophoretic buffers such as TBE and TAE, eliminating the need to perform a buffer exchange step before digestion. PR1MA™ β - Agarase has higher thermal stability than other commercially available β - agarases, with a broad range of activity between 42°C and 50°C. Higher temperature digestion reduces the chances of residual agarose gelling at the end of the reaction, resulting in higher recovery of DNA or RNA.

Benefits

• Complete digestion of agarose, with no agarose fragments, left after the digestion
• Obtain DNA or RNA faster - no buffer exchange needed, with direct digestion in TAE or TBE
• Concentration: 1000 u / mL
• Operating temperature: 42°C to 50°C
• Recommended temperature: 50°C
• Storage temperature: -20°C 

Storage Buffer

• 50% glycerol
• 50 mM Tris-HCI
• 50 mM KCI
• 1 mM DTT
• 0.1  mM EDTA
• pH = 7.5 

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.

Proudly made in the USA! Click here for more made in America products!
  

PrecisionPak Plant Matter DNA Kit

All-inclusive kit, from lysis to extraction, including protocol, lysis beads, all buffers and reagents, to ensure high quality DNA.

The PrecisionPak Plant Matter DNA extraction kit contains a combination of lysis beads preloaded in sample tubes and lysis buffer for thorough homogenization and high yield. The buffers' formulations are environmentally friendly and do not require ethanol, phenol, chloroform, and isopropanol. The extraction kit is innovatively designed to be faster and more efficient than spin-column methods.

Tough Plant Matter DNA Kit available for: Barley, Beans, Blueberry, Cannabis, Chickpeas, Cloves, Conifer Needles, Corn, Dried Soy Beans, Fungi (M.phaseolina), Ginger Rhizome, Horseradish Root, Leaves, Leek, Malanga (Tuber), Microsporidia, Moss, Mung Beans, Mycelia, N. benthamiana, Pine Nut, Potato, Rice, Soy Bean Plant, Spelt, Sugar Cane Pulp, Sunflower Seeds, Sorghum, Tobacco, Tomato Root, Tomato Seeds, Wheat Berries.

Soft Plant Matter DNA Kit available for: Algae, A thaliana, and Phytoplankton.   
 

PrecisionPak Animal Tissue RNA Kit

All-inclusive kit, from lysis to extraction, including protocol, lysis beads, all buffers and reagents, magnetic extraction beads, and additional reagents to ensure high-quality RNA.

The PrecisionPak Animal Tissue RNA extraction kit contains a combination of lysis beads preloaded in sample tubes and lysis buffer for thorough homogenization and high yield. Magnetic bead technology and additional reagents such as Proteinase K and DNase ensure very high-quality RNA. The magnetic bead technology is innovatively designed to be faster and more efficient than spin-column methods.

Tough Animal Tissue RNA Kits available for: Aorta, Bladder, Cartilage, Cecum, Colon, Diaphragm, Duodenum, Ear, Eye, Feces, Heart, Intestinal Mucosa, Intestine, Jejunum, Larynx, Meconium, Mouse Femur and Tibia, Muscle, Nasal, Olfactory Mucosa, Oropharynx, Placenta, Polyp, Skin, Spinal Cord, Stomach, Tail Snips, Testes, Thalamus, Toe Clip, Tongue, Trachea, Tumor, Umbilical Cord, and Uterus.

Soft Animal Tissue RNA Kit available for: Adipose, Adrenal Gland, Blood, Blood Vessels, Brain, Cerebral Fluid, Gall Bladder, Hypothalamus, Kidney, Liver, Lung, Lymph Node, Marrow, Nerve, Pancreas, Pharynx, Pituitary Gland, Salivary Gland, Spleen, Thymus, and Thyroid.   
 

PrecisionPak Animal Tissue DNA Kit

All-inclusive kit, from lysis to extraction, including protocol, lysis beads, all buffers and reagents, magnetic extraction beads, and additional reagents to ensure high-quality DNA.

The PrecisionPak Animal Tissue DNA extraction kit contains a combination of lysis beads preloaded in sample tubes and lysis buffer for thorough homogenization and high yield. Magnetic bead technology, coupled with additional reagents such as Proteinase K and RNase ensures very high-quality DNA. The magnetic bead technology is innovatively designed to be faster and more efficient than spin-column methods.

Tough Animal Tissue DNA Kit available for: Aorta, Bladder, Cartilage, Cecum, Colon, Diaphragm, Duodenum, Ear, Eye, Feces, Heart, Intestinal Mucosa, Intestine, Jejunum, Larsynx, Meconium, Mouse Femur and Tibia, Muscle, Nasal, Olfactory Mucosa, Oropharynx, Placenta, Polyp, Skin, Spinal Cord, Stomach, Tail Snips, Testes, Thalamus, Toe Clip, Tongue, Trachea, Tumor, Umbilical Cord, and Uterus.

Soft Animal Tissue DNA Kit available for: Adipose, Adrenal Gland, Blood, Blood Vessels, Brain, Cerebral Fluid, Gall Bladder, Hypothalamus, Kidney, Liver, Lung, Lymph Node, Marrow, Nerve, Pancreas, Pharynx, Pituitary Gland, Salivary Gland, Spleen, Thymus, and Thyroid.   
 

PrecisionPak Small Organism DNA Kit

All-inclusive kit, from lysis to extraction, including protocol, lysis beads, all buffers and reagents, magnetic extraction beads, and additional reagents to ensure high quality DNA.

The PrecisionPak Small Organism DNA extraction kit contains a combination of lysis beads preloaded in sample tubes and lysis buffer for thorough homogenization and high yield. Magnetic bead technology, coupled with additional reagents such as Proteinase K and RNase ensure very high-quality DNA. The magnetic bead technology is innovatively designed to be faster and more efficient than spin-column methods.

Tough Small Organism DNA Kit available for: Caterpillar, Crayfish, Crickets, Fruit Fly (Drosophila), Honeybee, Mosquito, Shrimp, Ticks, Zebrafish (D. rerio), Xenopus Tissue.

Soft Small Organism DNA Kits available for: Hookworm, Nematode (B. xylophilus), Roundworm (C. elegans), and Zooplankton.   
 

PrecisionPak Small Organism RNA Kit

All-inclusive kit, from lysis to extraction, including protocol, lysis beads, all buffers and reagents, magnetic extraction beads, and additional reagents to ensure high-quality RNA.

The PrecisionPak Small Organism RNA extraction kit contains a combination of lysis beads preloaded in sample tubes and lysis buffer for thorough homogenization and high yield. Magnetic bead technology, coupled with additional reagents such as Proteinase K and DNase ensures very high-quality RNA. The magnetic bead technology is innovatively designed to be faster and more efficient than spin-column methods.

Tough Small Organism RNA Kits available for: Caterpillar, Crayfish, Crickets, Fruit Fly (Drosophila), Honeybee, Mosquito, Shrimp, Ticks, Zebrafish (D. rerio), Xenopus Tissue.

Soft Small Organism RNA Kit available for: Hookworm, Nematode (B. xylophilus), Roundworm (C. elegans), and Zooplankton.   
 

Notice for U.S. Customers – RWD Consumables
Due to changes in U.S.–China tariffs, we will pause sales of these items after Tuesday, April 22, until further notice.
This is in anticipation of the expected De Minimis rule change on May 2.

For more information or alternative product recommendations, please contact us.

RWD Cell Separation Assembly Kit

Applications include immunology research, oncology research, neuroscience, stem cell research, and cell therapy.

RWD magnetic microbead cell separation products consist of Nano-Bead Cell Separation Platform and Nano-Beads (Column Based and Column free). Nano-sized magnetic beads do not need elution, and the separated cells can be directly used in downstream experiments such as flow cytometry, cell culture, single-cell sequencing, and more.

• High purity, high viability, and high recovery rate
• Obtained cells are compatible with downstream applications
• Mild, non-toxic, biodegradable

Kit Includes

• 1 box of LarSep Columns (25/pk)
• 1 LSC Separator (single channel)
• 1 Separation Stand

Work Flow

• Antibody incubation: Incubate the antibody with cell suspension at 4ºC for 10-15 mins.
• Microbead incubation: Incubate the magnetic microbeads with cell suspension at 4ºC for 10-15 mins.
• Magnetic separation: Put the separation column into the magnetic field and add the cell suspension into the column.
• Only cells labeled with magnetic beads will be captured in the column.
• Elution: Remove the column from the magnetic field and add a buffer to elute the positive cells.

Notice for U.S. Customers – RWD Consumables
Due to changes in U.S.–China tariffs, we will pause sales of these items after Tuesday, April 22, until further notice.
This is in anticipation of the expected De Minimis rule change on May 2.

For more information or alternative product recommendations, please contact us.

RWD Human Cell Separation Kits

CD3+ Cell Separation Kit - K1201-10

For positive selection or depletion of CD3+ T cells from peripheral blood, thymus, lymphoid, and tumor tissue.

CD4+ Cell Separation Kit - K1202-10

For positive selection or depletion of T helper cells (Th) from human peripheral blood, umbilical cord blood, lymph nodes, thymus, spleen, skin, and other samples.

CD8+ Cell Separation Kit - K1203-10

For positive selection or depletion of cytotoxic T cells (Tc) from human peripheral blood, umbilical cord blood, lymph nodes, thymus, spleen, skin, and other samples.

CD14+ Cell Separation Kit - K1204-10

For positive selection or depletion of monocytes from human peripheral blood, umbilical cord blood, spleen, lymph nodes, and other samples.

CD45+ Cell Separation Kit - K1205-10

For positive selection or depletion of white blood cells from human peripheral blood and lymphoid tissue samples.

CD19+ Cell Separation Kit - K1206-10

For positive selection or depletion of B cells from human peripheral blood, lymph nodes, bone marrow, and other single-cell suspensions.

Human NK Cell Isolation Kit - K1207-10

For negative selection or depletion of NK cells from single cell suspension of human peripheral blood. Non-NK cells, i.e., T cells, B cells, stem cells, dendritic cells, monocytes, granulocytes, and red blood cells are labeled by biotin antibodies coupled to magnetic beads. High purity NK cells are obtained by isolation of magnetic bead labeled cells.

*All kits are for research use only.

Specifications
 

Item #	Cell Type	Size	Capacity
K1201-10	T Cells	2 x 1 mL	For 1 x 10^9 Total Cells
K1202-10	T Helper Cells	2 x 1 mL
K1203-10	Cytotoxic T Cells	2 x 1 mL
K1204-10	Monocytes	2 x 1 mL
K1205-10	Leukocytes	2 x 1 mL
K1206-10	B Cells	2 x 1 mL
K1207-10	NK Cells	2 x 2 mL

 

Notice for U.S. Customers – RWD Consumables
Due to changes in U.S.–China tariffs, we will pause sales of these items after Tuesday, April 22, until further notice.
This is in anticipation of the expected De Minimis rule change on May 2.

For more information or alternative product recommendations, please contact us.

RWD High Activity Enzymatic Digestion Kits

Optimized enzymatic hydrolysis formulation for efficient and gentle digestion. Preserves the integrity of surface antigenic epitopes. Store in refrigerators at 4°C or -20°C.

• Targeted optimization of enzymatic hydrolysis formula can ensure high cell viability.
• Preserving cell surface epitopes leads to no effect on downstream experiments.

Notice for U.S. Customers – RWD Consumables
Due to changes in U.S.–China tariffs, we will pause sales of these items after Tuesday, April 22, until further notice.
This is in anticipation of the expected De Minimis rule change on May 2.

For more information or alternative product recommendations, please contact us.   

RWD Mouse and Microglia Cell Separation Kits

Mouse CD3+ Cell Separation Kit - K1301-10

For positive selection or depletion of mature T lymphocytes from mouse lymph nodes, thymus, tumors, and other samples.

Mouse CD4+ Cell Separation Kit - K1302-10

For positive selection or depletion of T helper cells (Th) from mouse lymph nodes, thymus, spleen, skin, and other samples.

Mouse CD8+ Cell Separation Kit - K1303-10

For positive selection or depletion of cytotoxic T cells (Tc) from mouse thymus, lymph nodes, spleen, and other samples.

Mouse CD45+ Cell Separation Kit - K1304-10

For positive selection or depletion of white blood cells from mouse lymphoid tissue or non-lymphoid tissue.

Mouse CD19+ Cell Separation Kit - K1305-10

For positive selection or depletion of B cells from mouse spleen and other samples.

CD11b+ (Microglia) cell separation Kit - K1306-10

For positive selection or depletion of microglia from human, adult mice, or rats brain tissue.

Specifications
 

Item #	Cell Type	Size	Capacity
K1301-10	T Lymphocytes	2 x 1 mL	For 1*10^9 Total Cells
K1302-10	T Helper Cells		For 1*10^9 Total Cells
K1303-10	Cytotoxic T Cells		For 1*10^9 Total Cells
K1304-10	Leukocytes		For 1*10^9 Total Cells
K1305-20	B Cells		For 2*10^9 Total Cells
K1306-10	Myeloid Cells		For 1*10^9 Total Cells